scholarly journals Changes of Polyamines Content and Polyamine Oxidase Activity of Roots and Leaves During Drought Stress in Wheat Seedlings

2003 ◽  
Vol 27 (5) ◽  
pp. 655-660
Author(s):  
GUAN Jun-Feng ◽  
LIU Hai-Long ◽  
LI Guang-Min
Keyword(s):  
Drought Stress ◽  
Oxidase Activity ◽  
Polyamine Oxidase ◽  
Wheat Seedlings

Effects of Betaine on Chloroplast Protective Enzymes andpsbAGene Expression in Wheat Seedlings under Drought Stress

2013 ◽  
Vol 39 (7) ◽  
pp. 1319 ◽  
Author(s):  
Peng-Fei HOU ◽  
Jun-Qing MA ◽  
Peng-Fei ZHAO ◽  
Huan-Ling ZHANG ◽  
Hui-Jie ZHAO ◽  
...  
Keyword(s):  
Drought Stress ◽  
Wheat Seedlings ◽  
Protective Enzymes

scholarly journals Enhancement of drought tolerance in Triticum aestivum L. seedlings using Azospirillum brasilense NO40 and Stenotrophomonas maltophilia B11

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Wedad A. Kasim ◽  
Mohamed E. H. Osman ◽  
Mohamed N. Omar ◽  
Samar Salama
Keyword(s):  
Triticum Aestivum ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Azospirillum Brasilense ◽  
Stenotrophomonas Maltophilia ◽  
Protein Pattern ◽  
Triticum Aestivum L ◽  
Protein Patterns ◽  
Wheat Seedlings ◽  
Enhanced Production

Abstract Background The effectiveness of two PGPB; Azospirillum brasilense NO40 and Stenotrophomonas maltophilia B11 was investigated in enhancing the drought tolerance of wheat (Triticum aestivum L.) seedlings cultivar Gemiza9. The inoculated or uninoculated grains were sown in unsterilized sandy soil and watered normally untill the 8th day. Drought stress was initiated by completely withholding water for 7 days (until wilting). Samples were collected after 15 days from sowing to evaluate some growth criteria, damage and defense indicators and to analyze the roots’ protein pattern. Results The results showed that inoculating wheat seedlings with these strains significantly diminished the inhibitory effects of drought stress on the relative water content of roots, shoots and leaves; area of leaves; contents of pigments (chlorophyll a and b) and ascorbic acid; and on the protein patterns of roots. Moreover, the bacterial inoculation notably reduced the drought-induced damage indicated by lower leakage of electrolytes and less accumulation of Malondialdehyde and hydrogen peroxide, surprisingly with less enhanced production of proline and activities of catalase and peroxidase than their uninoculated counterparts. Under normal conditions, inoculating wheat plants with these PGPB resulted in significantly promoted growth and elevated contents of pigments and altered protein patterns of roots. Conclusion Overall, we can say that both Azospirillum brasilense NO40 and Stenotrophomonas maltophilia B11 were able to deactivate the growth inhibition in wheat seedlings to some extent, while maintaining a certain level of efficient protection against damage under drought stress.

Characterization of miRNAs and their target genes in He-Ne laser pretreated wheat seedlings exposed to drought stress

2018 ◽  
Vol 164 ◽  
pp. 611-617 ◽  
Author(s):  
Zongbo Qiu ◽  
Yanyan He ◽  
Yimeng Zhang ◽  
Junli Guo ◽  
Li Wang
Keyword(s):  
Drought Stress ◽  
Target Genes ◽  
Wheat Seedlings

Function Analysis of Drought Resistance Related Gene TaGAPCs and TaWRKYs in Wheat

2021 ◽  
Author(s):  
Qiuzhu Wang ◽  
Lin Zhang ◽  
Shushen Yang
Keyword(s):  
Transcription Factors ◽  
Drought Stress ◽  
Function Analysis ◽  
Severe Drought ◽  
Primary Metabolism ◽  
Wild Type ◽  
Drought Treatment ◽  
Biological Regulation ◽  
Wheat Seedlings ◽  
Differential Genes

Abstract Backgrounds: Wheat (Triticum aestivum L.) is one of the most important food crops in the world. It faces various abiotic stresses during its growth. Drought is one of the main factors limiting the growth and development of wheat. Severe drought stress will Lead to a decline in wheat production. Cytoplasmic glyceraldehyde-3-phosphate dehydrogenase (GAPC) is an important member of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) family, which is widely present in plant cytoplasm. Plants play an important role in the process of primary metabolism and stress resistance.Result: In this study, a comparative transcriptomic analysis of the TaGAPCs-RNAi strain of Changwu 134 and the wild-type wheat seedlings of Changwu 134 under natural drought conditions was carried out. A total of 30067 differentially expressed genes were screened in RNAi strains and wild-type strains, of which 19,959 genes were up-regulated in RNAi strains and 10,108 genes were down-regulated in transcription. GO analysis shows that differential genes are mainly enriched in biological regulation, cellular processes, metabolic processes, and responses to stimuli. KEGG analysis showed that the differential genes were mainly concentrated in the biosynthesis of phenylpropane, plant hormone signal transduction and flavonoid biosynthesis pathways. By analyzing the expression levels of differential transcription factors, the significantly down-regulated transcription factor WRKY family member TaWRKY2 / 22/28/29/33/40/47/52 in wheat was screened out. The TaWRKY28/33/40/47 gene silencing line was successfully obtained using the barley stripe mosaic virus (BSMV-VIGS) technology. The plants with TaWRKY28/33/40/47 gene silenced were subjected to natural drought treatment, and physiological and biochemical index tests were carried out. The results showed that the growth status of gene-silenced plants was worse than that of wild-type plants, and the relative water content and chlorophyll content decreased. The content of MDA, H2O2 and superoxide anion increases, the activity of antioxidant enzymes (SOD, POD, CAT) decreases, and the content of proline decreases. Conclusion: The results showed that TaGAPCs regulates the expression of some TaWRKYs transcription factors, activates antioxidant pathways, enhances tolerance of wheat to drought stress.

scholarly journals Drought stress response in winter wheat varieties – changes in leaf proteins and proteolytic activities

2020 ◽  
Vol 79 (2) ◽  
pp. 121-130
Author(s):  
Lyudmila Simova-Stoilova ◽  
Elisaveta Kirova ◽  
Dobrina Pecheva
Keyword(s):  
Drought Stress ◽  
Winter Wheat ◽  
Large Subunit ◽  
Rubisco Activase ◽  
Plant Performance ◽  
Strong Increase ◽  
Severe Drought ◽  
Wheat Varieties ◽  
Wheat Seedlings ◽  
Stress Coping Strategies

Radiation mutagenesis has been used in sustainable agriculture as a tool for increasing plant variability and providing new lines for selection. This necessitates a comparison, by using suitable stress markers, of the newly created lines with some well-established varieties, which are stress tolerant or susceptible. Drought is one of the most frequently encountered stresses with deleterious effects on plant performance and crop yield. Winter wheat seedlings (soil cultures at 3–4th leaf stage) from one mutant line (M181/1338K), one drought-tolerant (Guinness) and one sensitive variety (Farmer) were subjected to severe drought stress by water withholding, followed by recovery. Changes in leaf protein profiles, the amount of Rubisco large subunit (RLS), some specific chloroplast proteins such as Rubisco binding protein (RPB), Rubisco activase (RA), the chaperone subunit clpA/C of clp protease, as well as the activities of exo- and endo-proteases were analyzed. At the protein level, some differences were found in the drought response of genotypes – stability of RLS and RBP in M181/1338K and Guinness, diminution of RLS and increase in RBP in Farmer. RA presented strong up-regulation at recovery in Guinness but decreased in content under drought in M181/1338K and Farmer. Increase in ClpA/C level was found in all compared varieties under stress. Strong increase in total proteolytic activity was detected under drought only in Farmer. Inhibitory analysis revealed a predominance of cysteine and serine protease types. Aminopeptidase activities remained higher at recovery in M181/1338K and Farmer. Results are discussed in terms of genotype-linked different stress coping strategies.

A high level of polyamine oxidase activity in ascitic fluid of pancreatic origin

2000 ◽  
Vol 32 ◽  
pp. 226
Author(s):  
G.B. Bjelaković ◽  
M. Bjelaković ◽  
A. Nagorni ◽  
G. Bjelaković
Keyword(s):  
Ascitic Fluid ◽  
Oxidase Activity ◽  
Polyamine Oxidase ◽  
High Level ◽  
Pancreatic Origin

Does polyamine oxidase activity influence the oxidative metabolism of children who suffer of diabetes mellitus?

2010 ◽  
Vol 341 (1-2) ◽  
pp. 79-85 ◽  
Author(s):  
G. Bjelakovic ◽  
S. Beninati ◽  
B. Bjelakovic ◽  
D. Sokolovic ◽  
T. Jevtovic ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Oxidative Metabolism ◽  
Oxidase Activity ◽  
Polyamine Oxidase
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