scholarly journals CHAPERONE HSP70 MODULES THE PATHWAY OF RAW264.7 CELL ENDOCYTOSIS OF AT LPS-INDUCED ACTIVATION

2021 ◽  
pp. 22-26
Author(s):  
M.M. Yurinskaya ◽  
M.B. Evgenev ◽  
V.N. Afanasev ◽  
M.G. Vinokurov
Keyword(s):  
Raw264.7 Cell ◽  
Chaperone Hsp70

scholarly journals The Effects of Endocrine Disrupting Chemicals on Biomarkers of Inflammation Produced by Lipopolysaccharide Stimulated RAW264.7 Macrophages

2019 ◽  
Vol 16 (16) ◽  
pp. 2914
Author(s):  
Vedastus W. Makene ◽  
Edmund J. Pool
Keyword(s):  
Estrogen Receptor ◽  
Receptor Antagonist ◽  
Cell Cultures ◽  
Endocrine Disrupting Chemicals ◽  
Raw264.7 Cells ◽  
Inhibitory Effects ◽  
Receptor Antagonists ◽  
Estrogen Receptor Antagonist ◽  
Endocrine Disrupting ◽  
Raw264.7 Cell

Endocrine disrupting chemicals (EDCs) are common pollutants in the environment and can induce disruption of the endocrine and immune systems. The present study evaluated the effects of selected common environmental EDCs on secretion of inflammatory biomarkers by RAW264.7 cells. The EDCs investigated were Estradiol (E2), 5α-dihydrotestosterone (DHT), and Bisphenol A (BPA). To evaluate if the effects caused by EDCs were modulated by steroid hormone receptors, antagonists of estrogen and androgen receptors were used. The steroid receptor antagonists used were Tamoxifen, an estrogen receptor antagonist, and Flutamide, an androgen receptor antagonist. Secretion of biomarkers of inflammation, namely nitric oxide (NO) and interleukin 6 (IL-6), were monitored. The NO was determined using Griess reaction and IL-6 was measured by enzyme linked immunosorbent assay (ELISA). Although 5 μg/mL E2, DHT, and BPA were not toxic to RAW264.7 cell cultures, the same treatments significantly (p < 0.001) reduced both NO and IL-6 secretion by lipopolysaccharide (LPS)-stimulated RAW264.7 cell cultures. The suppression of NO and IL-6 secretion indicate inhibition of inflammation by DHT, E2, and BPA. The inhibitory effects of DHT, E2 and BPA are partially mediated via their cellular receptors, because the effects were reversed by their respective receptor antagonists. Flutamide reversed the effects of DHT, while Tamoxifen reversed the effects of E2 and BPA. In conclusion, E2, BPA, and DHT inhibit the synthesis of inflammation biomarkers by LPS-stimulated RAW264.7 cells. The inhibitory effects of EDCs can be partially reversed by the addition of an estrogen receptor antagonist for E2 and BPA, and an androgenic receptor antagonist for DHT. The inhibition of inflammatory response in stimulated RAW264.7 cells may be a useful bioassay model for monitoring estrogenic and androgenic pollutants.

HSP70 and lysosomal storage disorders: novel therapeutic opportunities

2010 ◽  
Vol 38 (6) ◽  
pp. 1479-1483 ◽  
Author(s):  
Nikolaj H.T. Petersen ◽  
Thomas Kirkegaard
Keyword(s):  
Heat Shock ◽  
Stress Responses ◽  
Molecular Chaperone ◽  
Heat Shock Protein 70 ◽  
Physiological Stress ◽  
Lysosomal Storage ◽  
Catabolic Enzymes ◽  
Chaperone Hsp70 ◽  
Storage Disorders ◽  
Novel Therapeutic

Lysosomes, with their arsenal of catabolic enzymes and crucial metabolic housekeeping functions are experiencing a revived research interest after having lived a rather quiet life for the last few decades. With the discovery of the interaction of the lysosomes with another ancient component of cellular homoeostasis, the molecular chaperone HSP70 (heat-shock protein 70), the stage seems set for further discoveries of the mechanisms regulating cellular and physiological stress responses to otherwise detrimental challenges.

scholarly journals MiR-155/GSK-3β mediates anti-inflammatory effect of Chikusetsusaponin IVa by inhibiting NF-κB signaling pathway in LPS-induced RAW264.7 cell

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yi Xin ◽  
Qin Yuan ◽  
Chaoqi Liu ◽  
Changcheng Zhang ◽  
Ding Yuan
Keyword(s):  
Signaling Pathway ◽  
Mouse Liver ◽  
Inflammatory Responses ◽  
Biological Activities ◽  
Raw264.7 Cells ◽  
Inflammation Model ◽  
Raw264.7 Cell ◽  
Anti Inflammatory ◽  
Gsk 3Β ◽  
Inflammatory Effect

Abstract It has been demonstrated that Chikusetsusaponin IVa (CsIVa) possesses abundant biological activities. Herein, using LPS to establish acute inflammation model of mouse liver and cell line inflammation model, we investigated whether miR-155/GSK-3β regulated NF-κB signaling pathway, and CsIVa exerted anti-inflammatory effects by regulating miR-155/GSK-3β signaling pathway. Our results showed that LPS induced high expression of miR-155 and miR-155 promoted macrophage activation through GSK-3β. In addition, CsIVa inhibited inflammatory responses in LPS-induced mouse liver and RAW264.7 cells. Furthermore, we demonstrated that CsIVa improved the inflammatory response in LPS-induced RAW264.7 cells by inhibiting miR-155, increasing GSK-3β expression, and inhibiting NF-κB signaling pathway. In conclusion, our study reveals that CsIVa suppresses LPS-triggered immune response by miR-155/GSK-3β-NF-κB signaling pathway.

scholarly journals A role for the chaperone Hsp70 in the regulation of border cell migration in the Drosophila ovary

2008 ◽  
Vol 125 (11-12) ◽  
pp. 1048-1058 ◽  
Author(s):  
Laura Cobreros ◽  
Ana Fernández-Miñán ◽  
Carlos M. Luque ◽  
Acaimo González-Reyes ◽  
María D. Martín-Bermudo
Keyword(s):  
Cell Migration ◽  
Border Cell ◽  
Border Cell Migration ◽  
Drosophila Ovary ◽  
Chaperone Hsp70

Co-expression of human chaperone Hsp70 and Hsdj or Hsp40 co-factor increases solubility of overexpressed target proteins in insect cells

2000 ◽  
Vol 1493 (1-2) ◽  
pp. 119-124 ◽  
Author(s):  
Naoaki Yokoyama ◽  
Mineo Hirata ◽  
Kenzo Ohtsuka ◽  
Yukihiro Nishiyama ◽  
Ken Fujii ◽  
...  
Keyword(s):  
Insect Cells ◽  
Target Proteins ◽  
Chaperone Hsp70

scholarly journals The molecular chaperone Hsp70 promotes the proteolytic removal of oxidatively damaged proteins by the proteasome

2016 ◽  
Vol 99 ◽  
pp. 153-166 ◽  
Author(s):  
Sandra Reeg ◽  
Tobias Jung ◽  
José P. Castro ◽  
Kelvin J.A. Davies ◽  
Andrea Henze ◽  
...  
Keyword(s):  
Molecular Chaperone ◽  
Chaperone Hsp70
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