scholarly journals COMPARATIVE ANALYSIS OF THE CONTENT OF PROTEIN COMPONENTS OF THE CYTOPLASMIC MEMBRANE OF RED BLOOD CELLS IN HEALTHY INDIVIDUALS AND PATIENTS WITH ULCERATIVE COLITIS

2019 ◽  
pp. 115-120 ◽  
Author(s):  
A.S. Sergeeva ◽  
T.S. Yankova ◽  
O.V. Say
Keyword(s):  
Ulcerative Colitis ◽  
Comparative Analysis ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Cytoplasmic Membrane ◽  
Healthy Individuals ◽  
Protein Components

scholarly journals The ontogeny of key endoplasmic reticulum proteins in human embryonic and fetal red blood cells

Blood ◽  
1996 ◽  
Vol 87 (2) ◽  
pp. 762-770 ◽  
Author(s):  
R Hume ◽  
A Burchell ◽  
BB Allan ◽  
CR Wolf ◽  
RW Kelly ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Cytochrome P450 ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Glucose Production ◽  
Uridine Diphosphate ◽  
Protein Components ◽  
Cytochrome P450 Isozymes ◽  
Endoplasmic Reticulum Proteins

Recently, using immunohistochemical methods, we surprisingly found that endoplasmic reticulum glucose-6-phosphatase is present in human embryonic and fetal red blood cells (RBCs) but not in adult RBCs. The fact that an endoplasmic reticulum enzyme, whose major site of expression in adults is the liver, is present in human embryonic and fetal RBCs, particularly nucleated cells, indicated that it would be sensible to determine whether these cells also contain other endoplasmic reticulum enzyme systems normally found in adult liver. Therefore, we have studied the expression of other endoplasmic reticulum proteins and found that human embryonic and fetal RBC precursors contain other protein components of the glucose-6- phosphatase system, ie, the phosphate and glucose transport proteins as well as other enzymes (eg, uridine diphosphate- glucuronosyltransferases, cytochrome P450 isozymes, nicotinamide adenine dinucleotide phosphate cytochrome P450 oxidoreductase, and prostaglandin H synthase). In addition, we also found the predominantly cytosolic markers 15-hydroxyprostaglandin dehydrogenase, prostaglandins PGE2 and 13,14-dihydro-15-keto-PGE2. The expression of key enzymes that control glucose production, detoxification of endobiotics and xenobiotics, and the regulation of prostaglandin levels in embryonic and early fetal RBCs means that these cells may have an important role in protecting the developing conceptus before it establishes an efficient circulation and before all tissues fully express their normal complement of these enzymes.

Association of ulcerative colitis and red blood cells coated with autoimmune antibody

1963 ◽  
Vol 8 (6) ◽  
pp. 537-544 ◽  
Author(s):  
J. A. Balint ◽  
W. J. Hammack ◽  
T. B. Patton
Keyword(s):  
Ulcerative Colitis ◽  
Red Blood Cells ◽  
Blood Cells

scholarly journals Blood bank storage of red blood cells increases RBC cytoplasmic membrane order and bending rigidity

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259267
Author(s):  
Sebastian Himbert ◽  
Syed M. Qadri ◽  
William P. Sheffield ◽  
Peter Schubert ◽  
Angelo D’Alessandro ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Storage Time ◽  
Cytoplasmic Membrane ◽  
Md Simulations ◽  
Coarse Grained ◽  
Small Contribution ◽  
Bending Rigidity ◽  
X Ray ◽  
Membrane Order

Blood banks around the world store blood components for several weeks ensuring its availability for transfusion medicine. Red blood cells (RBCs) are known to undergo compositional changes during storage, which may impact the cells’ function and eventually the recipients’ health. We extracted the RBC’s cytoplasmic membrane (RBCcm) to study the effect of storage on the membranes’ molecular structure and bending rigidity by a combination of X-ray diffraction (XRD), X-ray diffuse scattering (XDS) and coarse grained Molecular Dynamics (MD) simulations. Blood was stored in commercial blood bags for 2 and 5 weeks, respectively and compared to freshly drawn blood. Using mass spectrometry, we measured an increase of fatty acids together with a slight shift towards shorter tail lengths. We observe an increased fraction (6%) of liquid ordered (lo) domains in the RBCcms with storage time, and an increased lipid packing in these domains, leading to an increased membrane thickness and membrane order. The size of both, lo and liquid disordered (ld) lipid domains was found to decrease with increased storage time by up to 25%. XDS experiments reveal a storage dependent increase in the RBCcm’s bending modulus κ by a factor of 2.8, from 1.9 kBT to 5.3 kBT. MD simulations were conducted in the absence of proteins. The results show that the membrane composition has a small contribution to the increased bending rigidity and suggests additional protein-driven mechanisms.

scholarly journals Features of electric and viscoelastic parameters of erythrocytes in patients with inflammatory intestinal diseases

2021 ◽  
Vol 1 (5) ◽  
pp. 19-30
Author(s):  
M. V. Kruchinina ◽  
I. O. Svetlova ◽  
A. V. Azgaldyan ◽  
M. F. Osipenko ◽  
E. Yu. Valuiskikh ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Differential Diagnosis ◽  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Average Diameter ◽  
Acute Stage ◽  
Viscoelastic Parameters ◽  
Surface Character

The aim of this work is to study the features of the electrical and viscoelastic parameters of erythrocytes in patients with inflammatory bowel diseases (ulcerative colitis, Crohn’s disease, unclassified colitis), taking into account the stage of the disease for possible use in differential diagnosis.The electrical and viscoelastic parameters of erythrocytes were studied using dielectrophoresis in 109 patients with IBD, mean age 37,7 + 11,7 years (50 patients with ulcerative colitis (UC), 41 with Crohn’s disease (CD), 18 with unclassified colitis (UCC) and 53 conditionally healthy, comparable in age and sex with the main groups.Red blood cells of individuals with IBD differed from those in the comparison group by a smaller average diameter, an increased proportion of deformed, spherocytic cells with a changed surface character with a reduced ability to deform, a lower level of surface charge of cells, an altered membrane structure with an increased ability to conduct electric current, prone to destruction and the formation of aggregates (p <0,0001–0,05).Analysis in individual groups with IBD in the acute stage, taking into account the therapy, revealed significant differences between the forms of IBD: in patients with Crohn’s disease, in contrast to patients with UC, red blood cells had lower values of the amplitude of deformation, capacity, dipole moment, and velocity of movement of cells towards electrodes, the proportion of discocytes, polarizability at most of the frequencies of the electric field (p <0,00001–0,05). On the contrary, the summarized indicators of rigidity, viscosity, electrical conductivity, aggregation and destruction indices were higher in CD than in UC (p <0,0001–0,05). CD patients had a greater number of deformed cells with altered surface character (p <0,00001).The features of the electrical and viscoelastic parameters of erythrocytes in patients with differentnosological forms of IBD can be used for the differential diagnosis of ulcerative colitis and Crohn’s disease in case of colon lesions, in the long term — for verification of the diagnosis in unclassified colitis.

scholarly journals Passive haemagglutination test for human neurocysticercosis immunodiagnosis: II - Comparison of two standardized procedures for the passive haemagglutination reagent in the detection of anti-Cysticercus cellulosae antibodies in cerebrospinal fluids

1988 ◽  
Vol 30 (1) ◽  
pp. 57-62 ◽  
Author(s):  
Mirthes Ueda ◽  
Adelaide José Vaz ◽  
Eide Dias Camargo ◽  
Ana Maria Carvalho de Souza ◽  
Regina Maria Figueiredo Benelli ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Treated Group ◽  
Healthy Individuals ◽  
Saline Extract ◽  
Passive Haemagglutination ◽  
Haemagglutination Test ◽  
Human Red Blood Cells ◽  
Cysticercus Cellulosae ◽  
Level Of Significance

A comparison of two different standardized reagent procedures for the passive haemagglutination test (PHA) in the detection of specific antibody to Cysticercus cellulosae in cerebrospinal fluid (CSF) was carried out. The formaldehyde-treated group O Rh-human red blood cells (HuRBC) and glutaraldehyde-treated sheep red blood cells (SRBC) were the supplies for the reagents preparation and, in the tests, they were designated as PHA-1 and PHA-2, respectively. For both reagents the cells were coated with the cysticerci total saline extract (TS) antigen. PHA-1 and PHA-2 were assessed in a total of 204 CSF from patients with neurocysticercosis, from non-related infections and from healthy individuals. The positivity and specificity indices obtained were respectively 81.7% and 94.4% for PHA-1 and for PHA-2, 88.7% and 96.6%. Since no significant differences were observed between the results provided by two reagents, at level of significance of 0.05, either processes of cell sensitization can alternatively be used according to the own laboratory convenience.

Role of desamethasone 21P loaded on red blood cells in the therapy of paediatric ulcerative colitis

2008 ◽  
Vol 40 (10) ◽  
pp. A56
Author(s):  
F. Bracci ◽  
B. Papadatou ◽  
M.S. Basso ◽  
A. Diamanti ◽  
A. Filoni ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Red Blood Cells ◽  
Blood Cells

scholarly journals The Electrophoretie Mobility of Red Blood Cells of Normal Human Beings

Blood ◽  
1961 ◽  
Vol 17 (1) ◽  
pp. 119-124 ◽  
Author(s):  
JOHN ANGERS ◽  
ANTONIO ROTTINO
Keyword(s):  
Electric Field ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Human Beings ◽  
Healthy Individuals ◽  
Disease States ◽  
Electrophoretic Behavior ◽  
Normal Human ◽  
Healthy Persons

Abstract Values obtained for the electrophoretic behavior of the red blood cells of healthy individuals is presented. The technic and instrument are described in detail and the necessary attention to meticulous care is emphasized. The data presented show that in an electric field the mobility of the red blood cells of healthy persons is constant and reproducible. It was concluded that the method is extremely sensitive and precise and that it may prove of value in the study of various disease states.

Association of antibody-coated red blood cells with ulcerative colitis

1955 ◽  
Vol 19 (6) ◽  
pp. 887-894 ◽  
Author(s):  
Mortimer Lorber ◽  
Lawrence I. Schwartz ◽  
Louis R. Wasserman
Keyword(s):  
Ulcerative Colitis ◽  
Red Blood Cells ◽  
Blood Cells
Sign in / Sign up

Export Citation Format

Share Document