scholarly journals INFLUENCE OF SODIUM SELENITE ON THE VIABILITY OF MICE TESTICLE CANCER CELLS AND THE EXPRESSION OF mRNA OF SELV (SELENOPROTEIN V), TGR (THIOREDOXIN-GLUTATHION REDUCTASE) И GPX4 (GLUTATHION PEROXIDASE 4) IN THEM

2018 ◽  
pp. 137-141
Author(s):  
M.V. Goltyaev ◽  
E.G. Varlamova
Keyword(s):  
Cancer Cells ◽  
Sodium Selenite ◽  
Selenoprotein V

Treatment of lung cancer cells with cytotoxic levels of sodium selenite: Effects on the thioredoxin system

2008 ◽  
Vol 75 (11) ◽  
pp. 2092-2099 ◽  
Author(s):  
Markus Selenius ◽  
Aristi Potamitou Fernandes ◽  
Ola Brodin ◽  
Mikael Björnstedt ◽  
Anna-Klara Rundlöf
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Sodium Selenite ◽  
Lung Cancer Cells ◽  
Thioredoxin System

scholarly journals Sodium Selenite Accentuates the Therapeutic Effect of Adriamycin Prodrug (PADM) against Gastric Cancer

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Shengquan Tan ◽  
Jiapeng Mo ◽  
Zixiong Zhang ◽  
Chuying Huang ◽  
Yi Zou ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Sodium Selenite ◽  
Therapeutic Potential ◽  
Mitochondrial Fission ◽  
Combined Treatment ◽  
Mitochondrial Morphology ◽  
Gastric Cancer Cells ◽  
Antitumor Effects ◽  
And Migration

Selenium has remained a controversial character in cancer research. While its antitumor effects have been widely demonstrated, further evidence is required to establish it as a robust treatment regime. Sodium selenite (SS), an inorganic selenium, reportedly affected the proliferation and redifferentiation of gastric cancer cells, but whether it could act as a complement to conventional chemotherapeutic drugs for combination therapy is uncertain. Herein, SGC-7901 and MGC-803 gastric cancer cells were treated with PADM (Ac-Phe-Lys-PABC-ADM), a prodrug of doxorubicin/adriamycin (ADM), and the combined antitumor effects of the two drugs were evaluated. Characterization after treatment revealed that although PADM exhibited antitumor effects individually by inhibiting the proliferation and migration of gastric cancer cells and inducing apoptosis, the addition of SS significantly amplified these effects. Furthermore, gastric cancer cell apoptosis triggered by the combined treatment of SS and PADM may involve the participation of mitochondrial apoptosis, as evidenced by the changes in mitochondrial morphology and occurrence of mitochondrial fission. Collectively, SS could be a strong complementary drug that accentuates the therapeutic potential of PADM in gastric cancer treatment and management, and its significance could contribute to unique and innovative anticancer strategies.

scholarly journals Combination of Sodium Selenite and Doxorubicin Prodrug Ac-Phe-Lys-PABC-ADM Affects Gastric Cancer Cell Apoptosis in Xenografted Mice

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Bo Wu ◽  
Jian Ge ◽  
Zixiong Zhang ◽  
Chuying Huang ◽  
Xiaodan Li ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Sodium Selenite ◽  
Combined Treatment ◽  
Xenograft Model ◽  
Tumor Xenograft ◽  
Mitochondrial Apoptosis ◽  
Gastric Cancer Cells ◽  
Apoptosis Pathway

The incidence of gastric cancer is extremely high in China, prompting the development of effective therapeutic strategies. Sodium selenite (SS) affects the proliferation and redifferentiation of gastric cancer cells and the Adriamycin prodrug Ac-Phe-Lys-PABC-ADM (PADM) reduces toxicity in gastric cancer treatment. However, the mechanisms involved therein remain unclear. In this study, nude mice were transplanted with SGC-7901 gastric cancer cells to construct a tumor xenograft model. After administration of SS and PADM, tumor weight and size were reduced. In addition, the levels of alanine aminotransferase, aspartate transaminase, creatinine, and lactate dehydrogenase were decreased, indicating improved hepatic and renal function and inhibited cancer cell metabolism. Furthermore, combined treatment of SS and PADM downregulated the expression of cell cycle-related proteins (cyclin-dependent kinase 4, Ki67, cyclin E, and cyclin D1), elevated that of proapoptosis proteins (Bax, cleaved caspase-3, cleaved caspase-9, and P53), and upregulated that of mitochondrial apoptosis-associated proteins (apoptotic protease activating factor 1 and second mitochondria-derived activator of caspases). In conclusion, combined treatment of SS and PADM effectively promoted apoptosis in gastric cancer xenografts via the mitochondrial apoptosis pathway.

scholarly journals Selenite Induces Cell Cycle Arrest and Apoptosis via Reactive Oxygen Species-Dependent Inhibition of the AKT/mTOR Pathway in Thyroid Cancer

2021 ◽  
Vol 11 ◽  
Author(s):  
Zhen Cheng ◽  
Shuang Yu ◽  
Weiman He ◽  
Jie Li ◽  
Tianyi Xu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Cycle ◽  
Thyroid Cancer ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Sodium Selenite ◽  
Oxygen Species ◽  
Cycle Arrest ◽  
Intracellular Ros ◽  
Thyroid Cancer Cells

Thyroid cancer is the most common endocrine malignancy, and its incidence has increased in the past decades. Selenium has been shown to have therapeutic effects against several tumors. However, its role in thyroid cancer and its underlying molecular mechanism remains to be explored. In the present study, we demonstrated that sodium selenite significantly decreased cell viability and induced G0/G1 cell cycle arrest and apoptosis in thyroid cancer cells in a dose-dependent manner. Transcriptomics revealed that sodium selenite induced intracellular reactive oxygen species (ROS) by promoting oxidative phosphorylation. Increased intracellular ROS levels inhibited the AKT/mTOR signaling pathway and upregulated EIF4EBP3. Intracellular ROS inhibition by N-acetylcysteine (NAC) ameliorated the cellular effects of sodium selenite. The in vitro findings were reproduced in xenograft thyroid tumor models. Our data demonstrated that sodium selenite exhibits strong anticancer effects against thyroid cancer cells, which involved ROS-mediated inhibition of the AKT/mTOR pathway. This suggests that sodium selenite may serve as a therapeutic option for advanced thyroid cancer.

scholarly journals Increased sodium selenite cytotoxicity in thioredoxin reductase 1 knockdown cancer cells

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Ryuta Tobe ◽  
Min‐Hyuk Yoo ◽  
Noelia Fradejas‐Villar ◽  
Bradley A Carlson ◽  
Soledad Calvo ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Sodium Selenite ◽  
Thioredoxin Reductase ◽  
Thioredoxin Reductase 1

scholarly journals Protein-partners of selenoprotein SELM and the role of selenium compounds in regulation of its expression in human cancer cells

2019 ◽  
Vol 488 (2) ◽  
pp. 212-216
Author(s):  
E. G. Varlamova ◽  
M. V. Goltyaev ◽  
E. E. Fesenko
Keyword(s):  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Sodium Selenite ◽  
Cancer Cell Lines ◽  
Breast Adenocarcinoma ◽  
Selenium Compounds ◽  
Cytoplasmic Actin ◽  
Methylseleninic Acid ◽  
Fibrosarcoma Cells

The search of potential partners of human SELM in lysates of two cancer cell lines HT-1080 (fibrosarcoma) and MCF-7 (breast adenocarcinoma) was carried out. Two cytoplasmic actin isoforms: cytoplasmic actin 1 (cytoskeleton b-actin) and cytoplasmic actin 2 (cytoskeletal g-actin) was identified as partners. In addition, the influence of two widely used antitumor selenium compounds (sodium selenite and methylseleninic acid) on the expression SELM in cancer cells was studied. According to the results obtained by real-time PCR and Western blotting, we was concluded that 1 µM and 10 µM sodium selenite was not affected on the expression SELM in fibrosarcoma cells, whereas in breast adenocarcinoma cells 1 µM sodium selenite slightly increased of expression and 10 µM resulted in a significant decrease (about 2 times). Methylseleninic acid in both cancer cell lines increased the expression of SELM gene, the most pronounced effect was observed when fibrosarcoma cells were treated with 10 µM MSC (increased expression of the hSelm gene by almost 4 times).

scholarly journals Sodium Selenite Enhanced the Anti-proliferative Effect of MEK-ERK Inhibitor in Thyroid Cancer Cells

In Vivo ◽  
2019 ◽  
Vol 34 (1) ◽  
pp. 185-190 ◽  
Author(s):  
JONG BIN KIM ◽  
EUN YEOL YANG ◽  
JOOHYUN WOO ◽  
HYUNGJU KWON ◽  
WOOSUNG LIM ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Cancer Cells ◽  
Sodium Selenite ◽  
Proliferative Effect ◽  
Thyroid Cancer Cells

scholarly journals Antitumor Effects of Selenium

2021 ◽  
Vol 22 (21) ◽  
pp. 11844
Author(s):  
Seung Jo Kim ◽  
Min Chul Choi ◽  
Jong Min Park ◽  
An Sik Chung
Keyword(s):  
Radiation Therapy ◽  
Cancer Cells ◽  
Cancer Patients ◽  
Advanced Cancer ◽  
Sodium Selenite ◽  
Subsequent Treatment ◽  
Advanced Cancer Patients ◽  
Antitumor Effects ◽  
Hydrogen Selenide ◽  
High Doses

Functions of selenium are diverse as antioxidant, anti-inflammation, increased immunity, reduced cancer incidence, blocking tumor invasion and metastasis, and further clinical application as treatment with radiation and chemotherapy. These functions of selenium are mostly related to oxidation and reduction mechanisms of selenium metabolites. Hydrogen selenide from selenite, and methylselenol (MSeH) from Se-methylselenocyteine (MSeC) and methylseleninicacid (MSeA) are the most reactive metabolites produced reactive oxygen species (ROS); furthermore, these metabolites may involve in oxidizing sulfhydryl groups, including glutathione. Selenite also reacted with glutathione and produces hydrogen selenide via selenodiglutathione (SeDG), which induces cytotoxicity as cell apoptosis, ROS production, DNA damage, and adenosine-methionine methylation in the cellular nucleus. However, a more pronounced effect was shown in the subsequent treatment of sodium selenite with chemotherapy and radiation therapy. High doses of sodium selenite were effective to increase radiation therapy and chemotherapy, and further to reduce radiation side effects and drug resistance. In our study, advanced cancer patients can tolerate until 5000 μg of sodium selenite in combination with radiation and chemotherapy since the half-life of sodium selenite may be relatively short, and, further, selenium may accumulates more in cancer cells than that of normal cells, which may be toxic to the cancer cells. Further clinical studies of high amount sodium selenite are required to treat advanced cancer patients.

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