scholarly journals Intracellular Flow Cytometry Staining Protocol v2 (protocols.io.tknekve)

protocols.io ◽  
2018 ◽  
Author(s):  
Kelsey Knight
Keyword(s):  
Flow Cytometry ◽  
Flow Cytometry Staining ◽  
Staining Protocol

scholarly journals A new staining protocol for detection of murine antibody-secreting plasma cell subsets by flow cytometry

2017 ◽  
Vol 47 (8) ◽  
pp. 1389-1392 ◽  
Author(s):  
Katharina Pracht ◽  
Julia Meinzinger ◽  
Patrick Daum ◽  
Sebastian R. Schulz ◽  
Dorothea Reimer ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Plasma Cell ◽  
Staining Protocol ◽  
Murine Antibody

Intracellular Flow Cytometry Staining (Collection) v1

protocols.io ◽  
2016 ◽  
Author(s):  
BioLegend not provided ◽  
Inc not provided
Keyword(s):  
Flow Cytometry ◽  
Flow Cytometry Staining

scholarly journals Intracellular flow cytometry staining of antibody-secreting cells using phycoerythrin-conjugated antibodies: pitfalls and solutions

2022 ◽  
Author(s):  
Patrick Renner ◽  
Michale Crone ◽  
Matthew Kornas ◽  
KimAnh Trang Pioli ◽  
Peter Dion Pioli
Keyword(s):  
Flow Cytometry ◽  
Protein Expression ◽  
Critical Role ◽  
Cell Types ◽  
Intracellular Protein ◽  
Antibody Secreting Cell ◽  
Flow Cytometry Staining ◽  
Antibody Conjugates ◽  
Accurate Comparison ◽  
Antibody Secreting Cells

Antibody-secreting cells are terminally differentiated B cells that play a critical role in humoral immunity through immunoglobulin secretion along with possessing the potential to be long-lived. It is now appreciated that antibody-secreting cells regulate multiple aspects of biology through the secretion of various cytokines. In this regard, intracellular flow cytometry is a key tool used to assess the presence of intracellular proteins such as cytokines and transcription factors. Here, we showed that the use of phycoerythrin-containing antibody conjugates led to a false interpretation of antibody-secreting cell intracellular protein expression compared to other cell types. This was mainly due to the inappropriate retention of these antibodies specifically within antibody-secreting cells. Furthermore, we demonstrated how to reduce this retention which allowed for a more accurate comparison of intracellular protein expression between antibody-secreting cells and other cell types such as B lymphocytes. Using this methodology, our data revealed that spleen antibody-secreting cells expressed Toll-like receptor 7 as well as the pro-form of the inflammatory cytokine interleukin-1β.

Development of a Mechanism-Based, DNA Staining Protocol Using SYTOX Orange Nucleic Acid Stain and DNA Fragment Sizing Flow Cytometry

2000 ◽  
Vol 286 (1) ◽  
pp. 138-148 ◽  
Author(s):  
Xiaomei Yan ◽  
Robert C. Habbersett ◽  
Julia M. Cordek ◽  
John P. Nolan ◽  
Thomas M. Yoshida ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
Dna Staining ◽  
Staining Protocol ◽  
Dna Fragment ◽  
Nucleic Acid Stain

scholarly journals Q and B values are critical measurements required for inter-instrument standardization and development of multicolor flow cytometry staining panels

2014 ◽  
Vol 85 (12) ◽  
pp. 1037-1048 ◽  
Author(s):  
Stephen P. Perfetto ◽  
Pratip K. Chattopadhyay ◽  
James Wood ◽  
Richard Nguyen ◽  
David Ambrozak ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Multicolor Flow Cytometry ◽  
B Values ◽  
Flow Cytometry Staining
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