Rapid Resistance Testing (RRT) protocol for Rapid Detection of Resistance to Carbapenems and Cephalosporins in Enterobacteriaceae Using Liquid Chromatography Tandem Mass Spectrometry v1 (protocols.io.r56d89e)

protocols.io ◽  
2018 ◽  
Author(s):  
Manal Tadros ◽  
Alexander Romaschin
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Rapid Detection ◽  
Resistance Testing ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

scholarly journals Detection of Carbapenemase-Producing Bacteria by Using an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method

2013 ◽  
Vol 58 (2) ◽  
pp. 1231-1234 ◽  
Author(s):  
Anne Carricajo ◽  
Paul O. Verhoeven ◽  
Salim Guezzou ◽  
Nathalie Fonsale ◽  
Gerald Aubert
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Rapid Detection ◽  
Ultra Performance Liquid Chromatography ◽  
Tandem Mass ◽  
Spectrometry Method ◽  
Content Type ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

ABSTRACTThe emergence of carbapenemase-producing bacteria poses a new challenge in the management of antibiotic therapies for patients. This report describes a new method using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for rapid detection of carbapenemase activity in enterobacteria,Pseudomonas aeruginosa, andAcinetobacter baumannii. In a panel of 78 isolates, including 41 carbapenemase-producing strains, the ULPC-MS/MS assay showed 100% agreement with molecular characterization, whereas six carbapenemase-producing isolates were not detected by the modified Hodge test.

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