mRNA purification with Ambion Dynabeads v1 (protocols.io.pbrdim6)

protocols.io ◽  
2018 ◽  
Author(s):  
Magdalena Julkowska
Keyword(s):  
Mrna Purification

scholarly journals Design of in vitro Transcribed mRNA Vectors for Research and Therapy

2019 ◽  
Vol 73 (5) ◽  
pp. 391-394
Author(s):  
Marina Tusup ◽  
Lars E. French ◽  
Mara De Matos ◽  
David Gatfield ◽  
Thomas Kundig ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Messenger Rna ◽  
Untranslated Region ◽  
Open Reading Frame ◽  
Purification Method ◽  
Reading Frame ◽  
Mrna Purification ◽  
Functional Regions ◽  
In Cells

The use of in vitro transcribed messenger RNA (ivt mRNA) for vaccination, gene therapy and cell reprograming has become increasingly popular in research and medicine. This method can be used in vitro (transfected in cells) or administered naked or formulated (lipoplexes, polyplexes, and lipopolyplexes that deliver the RNA to specific organs, such as immune structures, the lung or liver) and is designed to be an immunostimulatory or immunosilent agent. This vector contains several functional regions (Cap, 5' untranslated region, open reading frame, 3' untranslated region and poly-A tail) that can all be optimised to generate a highly efficacious ivt mRNA. In this study, we review these aspects and report on the effect of the ivt mRNA purification method on the functionality of this synthetic transient genetic vector.

scholarly journals Cell type–specific mRNA purification by translating ribosome affinity purification (TRAP)

2014 ◽  
Vol 9 (6) ◽  
pp. 1282-1291 ◽  
Author(s):  
Myriam Heiman ◽  
Ruth Kulicke ◽  
Robert J Fenster ◽  
Paul Greengard ◽  
Nathaniel Heintz
Keyword(s):  
Affinity Purification ◽  
Cell Type ◽  
Mrna Purification ◽  
Cell Type Specific ◽  
Specific Mrna

scholarly journals RNase H/oligonucleotide-directed mRNA purification (ROMP) of apoll mRNA

1993 ◽  
Vol 21 (3) ◽  
pp. 765-766 ◽  
Author(s):  
Clinton C. MacDonald ◽  
David L. Williams
Keyword(s):  
Rnase H ◽  
Mrna Purification

AFFINITY TECHNIQUES IN THE ISOLATION OF SPECIFIC POLYSOMES AND mRNA: PURIFICATION OF RAT ALBUMIN mRNA

1978 ◽  
pp. 337-351
Author(s):  
John M. Taylor ◽  
Warren S.L. Liao ◽  
Tenny P.H. Tse ◽  
George H. Keller ◽  
Ardell Conn
Keyword(s):  
Mrna Purification ◽  
Affinity Techniques ◽  
Albumin Mrna

Lab-on-a-chip mRNA purification and reverse transcription via a solid-phase gene extraction technique

Lab on a Chip ◽  
2017 ◽  
Vol 17 (6) ◽  
pp. 1128-1136 ◽  
Author(s):  
Gergana G. Nestorova ◽  
Karl Hasenstein ◽  
Nam Nguyen ◽  
Mark A. DeCoster ◽  
Niel D. Crews
Keyword(s):  
Stainless Steel ◽  
Reverse Transcription ◽  
Solid Phase ◽  
Lab On A Chip ◽  
Extraction Technique ◽  
Mrna Purification ◽  
Integrated Extraction

We report a lab-on-a-chip method for integrated extraction and analysis of mRNA from 3D cells using 130 μm in diameter stainless steel needles.

scholarly journals The use of R-looping for structural gene identification and mRNA purification

1979 ◽  
Vol 6 (7) ◽  
pp. 2483-2497 ◽  
Author(s):  
John L. Woolford ◽  
Michael Rosbash
Keyword(s):  
Structural Gene ◽  
Gene Identification ◽  
Mrna Purification
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