RNA Imaging with MERFISH - Imaging v1 (protocols.io.mesc3ee)

protocols.io ◽  
2017 ◽  
Author(s):  
Jeffrey R ◽  
Xiaowei Zhuang
Keyword(s):  
Rna Imaging

Merging DNA Probes with Nanotechnology for RNA Imaging In Vivo

2021 ◽  
Vol 17 ◽  
Author(s):  
Chuangui Sheng ◽  
Bo Wu ◽  
Lele Li ◽  
Yuliang Zhao
Keyword(s):  
Molecular Beacon ◽  
Imaging Techniques ◽  
Disease Diagnosis ◽  
Dna Probes ◽  
Diagnosis And Treatment ◽  
Hybridization Chain Reaction ◽  
Specific Response ◽  
Biological Functions ◽  
Rna Imaging

Background: Imaging of RNA in vivo is of great significance for elucidating their biological functions, revealing mechanisms behind disease, and for further diagnosis and treatment. Over the past decade, a variety of DNA-based molecular imaging techniques have been developed for RNA imaging in living cells. Nevertheless, non-invasive imaging of RNA in animals is still limited. Methods: An overview of the literature involving RNA imaging in vivo based on the integration of DNA probes with nanotechnology has been reviewed. Results: Attributed to DNA’s designability of sequences and specificity of recognition, molecular beacon, strand displacement and hybridization chain reaction would confer quick, efficient and specific response to target RNA. Multifunctional nanomaterials provide powerful support for the intracellular delivery of such DNA probes with spatiotemporal control over their sensing function, thereby achieving RNA imaging in vivo. Conclusion: Merging DNA probes with nanotechnology has gained substantial prospects for RNA imaging in vivo, which not only helps us to better elucidate biological functions of RNA, but also provides valuable information for further disease diagnosis and treatment.

scholarly journals Live-Cell Single RNA Imaging Reveals Bursts of Translational Frameshifting

2019 ◽  
Vol 75 (1) ◽  
pp. 172-183.e9 ◽  
Author(s):  
Kenneth Lyon ◽  
Luis U. Aguilera ◽  
Tatsuya Morisaki ◽  
Brian Munsky ◽  
Timothy J. Stasevich
Keyword(s):  
Live Cell ◽  
Translational Frameshifting ◽  
Rna Imaging

scholarly journals A dimerization-based fluorogenic dye-aptamer module for RNA imaging in live cells

2019 ◽  
Vol 16 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Farah Bouhedda ◽  
Kyong Tkhe Fam ◽  
Mayeul Collot ◽  
Alexis Autour ◽  
Stefano Marzi ◽  
...  
Keyword(s):  
Live Cells ◽  
Rna Imaging

scholarly journals 4926High-throughput single-molecule RNA imaging analysis reveals spatial heterogeneity in heart failure

2018 ◽  
Vol 39 (suppl_1) ◽  
Author(s):  
M Satoh ◽  
S Nomura ◽  
T Fujita ◽  
T Ko ◽  
T Tobita ◽  
...  
Keyword(s):  
Heart Failure ◽  
Spatial Heterogeneity ◽  
Single Molecule ◽  
Imaging Analysis ◽  
Rna Imaging

Cascade Circuits on Self-Assembled DNA Polymers for Targeted RNA Imaging In Vivo

2020 ◽  
Vol 92 (24) ◽  
pp. 15953-15958
Author(s):  
Xueli Zhu ◽  
Bin Qu ◽  
Zhan-Ming Ying ◽  
Jin-Wen Liu ◽  
Zhenkun Wu ◽  
...  
Keyword(s):  
Self Assembled ◽  
Rna Imaging

scholarly journals Author Correction: Highly specific multiplexed RNA imaging in tissues with split-FISH

2020 ◽  
Vol 17 (9) ◽  
pp. 947-947 ◽  
Author(s):  
Jolene Jie Lin Goh ◽  
Nigel Chou ◽  
Wan Yi Seow ◽  
Norbert Ha ◽  
Chung Pui Paul Cheng ◽  
...  
Keyword(s):  
Rna Imaging

scholarly journals A Genetic Screen Based on in Vivo RNA Imaging Reveals Centrosome-Independent Mechanisms for Localizing gurken Transcripts in Drosophila

2014 ◽  
Vol 4 (4) ◽  
pp. 749-760 ◽  
Author(s):  
Rippei Hayashi ◽  
S. Mark Wainwright ◽  
Sophie J. Liddell ◽  
Sheena M. Pinchin ◽  
Stuart Horswell ◽  
...  
Keyword(s):  
Genetic Screen ◽  
Rna Imaging
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