Beta-glucuronidase (GUS) assay (adapted for Phaeodactylum tricornutum) v1

protocols.io ◽  
2017 ◽  
Author(s):  
Vincent A
Keyword(s):  
Phaeodactylum Tricornutum ◽  
Gus Assay

scholarly journals SPX-related genes regulate phosphorus homeostasis in the marine phytoplankton, Phaeodactylum tricornutum

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Kaidian Zhang ◽  
Zhi Zhou ◽  
Jiashun Li ◽  
Jingtian Wang ◽  
Liying Yu ◽  
...  
Keyword(s):  
Stress Responses ◽  
Phaeodactylum Tricornutum ◽  
Response Regulator ◽  
P Uptake ◽  
Negative Regulator ◽  
Marine Phytoplankton ◽  
Alkaline Phosphatases ◽  
Phosphate Starvation Response ◽  
Essential Nutrient ◽  
Global Oceans

AbstractPhosphorus (P) is an essential nutrient for marine phytoplankton. Maintaining intracellular P homeostasis against environmental P variability is critical for phytoplankton, but how they achieve this is poorly understood. Here we identify a SPX gene and investigate its role in Phaeodactylum tricornutum. SPX knockout led to significant increases in the expression of phosphate transporters, alkaline phosphatases (the P acquisition machinery) and phospholipid hydrolases (a mechanism to reduce P demand). These demonstrate that SPX is a negative regulator of both P uptake and P-stress responses. Furthermore, we show that SPX regulation of P uptake and metabolism involves a phosphate starvation response regulator (PHR) as an intermediate. Additionally, we find the SPX related genes exist and operate across the phytoplankton phylogenetic spectrum and in the global oceans, indicating its universal importance in marine phytoplankton. This study lays a foundation for better understanding phytoplankton adaptation to P variability in the future changing oceans.

scholarly journals Plastidial ACP Δ9‐desaturase modulates EPA biosynthesis and TAG accumulation in Phaeodactylum tricornutum

2021 ◽  
Author(s):  
Richard Smith ◽  
Juliette Jouhet ◽  
Chiara Gandini ◽  
Vladimir Nekrasov ◽  
Eric Marechal ◽  
...  
Keyword(s):  
Phaeodactylum Tricornutum ◽  
Δ9 Desaturase ◽  
Tag Accumulation

Characterisation of novel regulatory sequences compatible with modular assembly in the diatom Phaeodactylum tricornutum

2021 ◽  
Vol 53 ◽  
pp. 102159
Author(s):  
Matthias Windhagauer ◽  
Raffaela M. Abbriano ◽  
Justin Ashworth ◽  
Lorenzo Barolo ◽  
Ana Cristina Jaramillo-Madrid ◽  
...  
Keyword(s):  
Phaeodactylum Tricornutum ◽  
Regulatory Sequences ◽  
Modular Assembly

Observations on moulting in Antarctic Krill (Euphausia superba Dana)

1982 ◽  
Vol 33 (1) ◽  
pp. 71 ◽  
Author(s):  
T Ikeda ◽  
P Dixon
Keyword(s):  
Phaeodactylum Tricornutum ◽  
Antarctic Krill ◽  
Dry Weight ◽  
Euphausia Superba ◽  
Dunaliella Tertiolecta ◽  
Antarctic Ocean ◽  
Fish Food ◽  
Antarctic Waters ◽  
The Antarctic ◽  
Antarctic Krill Euphausia Superba

Live E. superba were transported from Antarctic waters to a tropical laboratory where observations at the temperature of -0.5�C (0 to - 1.0�C), were made of intermoult period of specimens fed a mixture of microalgae (Dunaliella tertiolecta and Phaeodactylum tricornutum) or artificial pet fish food or starved. Mean intermoult period was 26.4-27.1 days for fed specimens and 29.6 days for starved specimens, with no relation to the size of specimens. The moult accounted for a loss of 2.63-4.35% of animal dry weight, which is equivalent to 1.1-1.8% of animal nitrogen or 1.4-2.3% of animal carbon. The contribution of moults to detritus in the Antarctic Ocean was estimated as 0.11 g C m-2 per year.

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