Digestion Mixture for M0302 (v2) v1

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2016 ◽  
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Digestion Mixture

scholarly journals THE ESTIMATION OF ACTIVE NATIVE TRYPSIN IN THE PRESENCE OF INACTIVE DENATURED TRYPSIN

1933 ◽  
Vol 17 (2) ◽  
pp. 159-164 ◽  
Author(s):  
M. L. Anson ◽  
A. E. Mirsky
Keyword(s):  
Protein Solutions ◽  
Digestion Mixture ◽  
Tryptic Activity

Inactive denatured trypsin changes into active native trypsin in the protein solutions which have been used to estimate tryptic activity. If the digestion mixture, however, is alkaline enough and contains enough urea this change does not take place. Such a digestion mixture can be used to estimate active native trypsin in the presence of inactive denatured trypsin.

Digestion of Sugar Beet Leaves for Atomic Absorption Spectroscopy

1969 ◽  
Vol 23 (4) ◽  
pp. 354-357 ◽  
Author(s):  
Merle Harrison ◽  
Charles Andre
Keyword(s):  
Sugar Beet ◽  
Atomic Absorption ◽  
Absorption Spectroscopy ◽  
Atomic Absorption Spectroscopy ◽  
Sodium Molybdate ◽  
Plant Materials ◽  
Digestion Mixture ◽  
Interfering Ions

A modified digestion mixture utilizing sodium molybdate as a catalyst is described for plant materials. In atomic absorption spectroscopy, special attention is given to calcium analyses using this mixture, because of various interfering ions. The digestion mixture is suitable for numerous other cation determinations. Also, a method of increasing the sensitivity for copper by adding methanol is described.

scholarly journals A continuous in vitro method for estimation of the bioavailability of minerals and trace elements in foods: application to breads varying in phytic acid content

1993 ◽  
Vol 69 (3) ◽  
pp. 849-861 ◽  
Author(s):  
Mechteldis G. E. Wolters ◽  
Hendrika A. W. Schreuder ◽  
Grietje Van Den Heuvel ◽  
Henk J. Van Lonkhuijsen ◽  
Ruud J. J. Hermus ◽  
...  
Keyword(s):  
Trace Elements ◽  
Phytic Acid ◽  
Acid Content ◽  
Equilibrium Dialysis ◽  
Phytic Acid Content ◽  
Different Types ◽  
Digestion Mixture ◽  
Minerals And Trace Elements

A continuous in vitro method for the estimation of the bioavailability of minerals and trace elements is presented. This in vitro method is believed to be more representative of in vivo physiological conditions than in vitro methods based on equilibrium dialysis, because dialysable components are continuously removed from the pancreatic digestion mixture. The continuous in vitro method is compared with the equilibrium in vitro method with respect to the dialysability of Ca, Mg, Fe, Cu and Zn from eight different types of bread (varying in phytic acid content). The results show a pronounced effect of continuous removal of dialysable components from the pancreatic digestion mixture on the dialysability of minerals and trace elements. Furthermore, removal of dialysable components influences the effect of phytic acid on the bioavailability of minerals and trace elements. For these two reasons the importance of removal of dialysable components in vitro for the estimation of bioavailahility in vivo needs further investigation. The bioavailability of minerals and trace elements from bread samples is not related to the phytic acid content only. Therefore, the effect of phytic acid on the bioavailability of minerals and trace elements cannot be studied separately from the effects of other components on bioavailability.

scholarly journals EFFECTS OF TRYPSIN DIGESTION ON FLAGELLAR STRUCTURES AND THEIR RELATIONSHIP TO MOTILITY

1973 ◽  
Vol 58 (3) ◽  
pp. 618-629 ◽  
Author(s):  
Keith E. Summers ◽  
I. R. Gibbons
Keyword(s):  
Electron Microscope ◽  
Trypsin Digestion ◽  
Shearing Force ◽  
Digestion Mixture ◽  
Radial Spokes ◽  
Time Periods ◽  
Dynein Arms ◽  
Cross Bridges ◽  
Sea Urchin Sperm ◽  
Bending Wave

Flagellar axonemes isolated from sea urchin sperm were digested with trypsin for various time periods. The course of digestion was monitored turbidimetrically and was found to take two different courses depending on the presence or absence of ATP in the digestion mixture. It was found that ATP induced active disintegration of the axonemes after slight digestion. Samples of the digested axonemes were examined with the electron microscope to determine the effects of trypsin digestion on the substructures of the axonemes. The rate at which trypsin sensitized the axonemes to ATP paralleled the rate at which it damaged the radial spokes and the nexin links, while the dynein arms were removed much more slowly. The results suggest that inactive dynein arms form cross bridges between the adjacent doublet tubules in digested axonemes, and that when activated by the addition of ATP, they induce an active shearing force between adjacent doublets. The radial spokes and the nexin links are not directly involved in the production of mechanical force, but they may participate in regulating the sliding between tubules to produce a propagated bending wave.

X-ray Spectrographic Microanalysis of Human Urine for Arsenic

1974 ◽  
Vol 28 (2) ◽  
pp. 165-170 ◽  
Author(s):  
James C. Mathies
Keyword(s):  
Standard Deviation ◽  
Human Body ◽  
Human Urine ◽  
Test Paper ◽  
X Ray ◽  
Paper Disk ◽  
Filter Paper Disk ◽  
Relative Standard ◽  
Digestion Mixture

A rapid, precise, and interference-free x-ray spectrographic procedure for the determination of arsenic in human body fluids and tissues has been devised. The organic material in the sample is oxidized, and the arsenic is released by a simplified rapid wet-washing technique using nitric-sulfuric-perchloric acids. The arsenic in the digestion mixture is converted to arsine and collected quantitatively on a silver nitrate-impregnated filter paper disk using a new and convenient sub-micro modification of the Gutzeit arsine generator. The arsenic in the test paper is rapidly and nondestructively quantitated to the nearest 0.1 µg in the x-ray spectrograph. The method is relatively free of the interferences usually associated with Gutzeit and colorimetric techniques for arsenic. The precision of the method is indicated by the fact that 5 µg of arsenic can be quantitated with a relative standard deviation of 2.5% or less. Normal levels of arsenic in human urine have been redetermined.

Intracellular Localization of Acid Peptide Hydrolases and Several Other Acid Hydrolases in the Leaf of Pea (Pisum sativum L.)

1982 ◽  
Vol 9 (3) ◽  
pp. 353 ◽  
Author(s):  
ER Noble ◽  
MJ Dalling
Keyword(s):  
Crude Extract ◽  
Intracellular Localization ◽  
Acid Proteinase ◽  
Phenylmethylsulfonyl Fluoride ◽  
Acid Hydrolases ◽  
Pisum Sativum L ◽  
Acid Carboxypeptidase ◽  
Digestion Mixture ◽  
Protoplast Preparation ◽  
Peptide Hydrolases

Protoplasts and vacuoles were prepared from pea (P. sativum L.) leaves to determine the acid-hydrolase content of the vacuole. The protoplasts and vacuoles were isolated on sucrose-sorbitol gradients. We conclude that the protoplast preparation was free of enzyme contamination arising from the digestion mixture, after comparing the specific activities of the crude extract and of the isolated protoplasts, and the response to the thiol inhibitor p-chloromercuribenzoate of the crude extract and protoplast acid proteinase with that of the Cellulysin enzyme. N-acetyl-β-D-glucosaminidase, α-mannosidase, acid carboxypeptidase and acid proteinase appeared to be located entirely in the vacuole. Approximately 60% of the acid-phosphatase and 27% of the phosphodiesterase activities were found in the vacuole. We conclude that the acid proteinase and the acid carboxypeptidase are separate enzymes in pea leaves, based on their different sensitivities to the inhibitors phenylmethylsulfonyl fluoride and p-chloromercuribenzoate.

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