Buffers for Chloroplast Isolation from Diatoms v1

protocols.io ◽  
2015 ◽  
Author(s):  
P Dreux ◽  
Bethany D
Keyword(s):  
Chloroplast Isolation

scholarly journals Methodology: an optimized, high-yield tomato leaf chloroplast isolation and stroma extraction protocol for proteomics analyses and identification of chloroplast co-localizing proteins

Plant Methods ◽  
2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Oindrila Bhattacharya ◽  
Irma Ortiz ◽  
Linda L. Walling
Keyword(s):  
Fruit Ripening ◽  
High Yield ◽  
Published Data ◽  
Dynamic Interactions ◽  
Chloroplast Isolation ◽  
Stress Signals ◽  
Chloroplast Stroma ◽  
Stromal Proteins ◽  
Cellular Components ◽  
Chloroplast Proteome

Abstract Background Chloroplasts are critical organelles that perceive and convey metabolic and stress signals to different cellular components, while remaining the seat of photosynthesis and a metabolic factory. The proteomes of intact leaves, chloroplasts, and suborganellar fractions of plastids have been evaluated in the model plant Arabidopsis, however fewer studies have characterized the proteomes of plastids in crops. Tomato (Solanum lycopersicum) is an important world-wide crop and a model system for the study of wounding, herbivory and fruit ripening. While significant advances have been made in understanding proteome and metabolome changes in fruit ripening, far less is known about the tomato chloroplast proteome or its subcompartments. Results With the long-term goal of understanding chloroplast proteome dynamics in response to stress, we describe a high-yielding method to isolate intact tomato chloroplasts and stromal proteins for proteomic studies. The parameters that limit tomato chloroplast yields were identified and revised to increase yields. Compared to published data, our optimized method increased chloroplast yields by 6.7- and 4.3-fold relative to published spinach and Arabidopsis leaf protocols, respectively; furthermore, tomato stromal protein yields were up to 79-fold higher than Arabidopsis stromal proteins yields. We provide immunoblot evidence for the purity of the stromal proteome isolated using our enhanced methods. In addition, we leverage our nanoliquid chromatography tandem mass spectrometry (nanoLC–MS/MS) data to assess the quality of our stromal proteome. Using strict criteria, proteins detected by 1 peptide spectral match, by one peptide, or were sporadically detected were designated as low-level contaminating proteins. A set of 254 proteins that reproducibly co-isolated with the tomato chloroplast stroma were identified. The subcellular localization, frequency of detection, normalized spectral abundance, and functions of the co-isolating proteins are discussed. Conclusions Our optimized method for chloroplast isolation increased the yields of tomato chloroplasts eightfold enabling the proteomics analysis of the chloroplast stromal proteome. The set of 254 proteins that co-isolate with the chloroplast stroma provides opportunities for developing a better understanding of the extensive and dynamic interactions of chloroplasts with other organelles. These co-isolating proteins also have the potential for expanding our knowledge of proteins that are co-localized in multiple subcellular organelles.

scholarly journals NaOH low-salt method for chloroplast isolation and highly pure cpDNA preparation from Aeluropus littoralis

2018 ◽  
Vol 6 (9) ◽  
pp. e01183
Author(s):  
Najmeh Nasiri ◽  
Ghorbanali Nematzadeh ◽  
Vahid Shariati-Joni ◽  
Hamid Najafi-Zarrini
Keyword(s):  
Aeluropus Littoralis ◽  
Low Salt ◽  
Chloroplast Isolation

scholarly journals Chloroplast Isolation in Nonaqueous Media.

1959 ◽  
Vol 34 (1) ◽  
pp. 56-61 ◽  
Author(s):  
C. Ralph Stocking
Keyword(s):  
Nonaqueous Media ◽  
Chloroplast Isolation

Foliar flavonoid distribution during Spinacia chloroplast isolation

Planta ◽  
1981 ◽  
Vol 151 (4) ◽  
pp. 309-313 ◽  
Author(s):  
Yvette Charriere-Ladreix ◽  
Michel Tissut
Keyword(s):  
Chloroplast Isolation
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