Anti-Neu5Gc Antibody Kit Protocol - Flow Cytometry v1

protocols.io ◽  
2016 ◽  
Author(s):  
Kelsey Miller
Keyword(s):  
Flow Cytometry ◽  
Protocol Flow

scholarly journals iGEM Calibration Protocol - Flow Cytometry Cell Size v1 (protocols.io.2pegdje)

protocols.io ◽  
2019 ◽  
Author(s):  
Jacob Beal ◽  
Cheryl Telmer ◽  
Richard Tennant ◽  
Paul Rutten
Keyword(s):  
Flow Cytometry ◽  
Cell Size ◽  
Calibration Protocol ◽  
Protocol Flow

iGEM Calibration Protocol - Flow Cytometry Cell Size v1 (protocols.io.5ncg5aw)

protocols.io ◽  
2019 ◽  
Author(s):  
Jacob Beal ◽  
Cheryl Telmer ◽  
Richard Tennant ◽  
Paul Rutten
Keyword(s):  
Flow Cytometry ◽  
Cell Size ◽  
Calibration Protocol ◽  
Protocol Flow

scholarly journals A Simple and Effective Flow Cytometry-Based Method for Identification and Quantification of Tissue Infiltrated Leukocyte Subpopulations in a Mouse Model of Peripheral Arterial Disease

2020 ◽  
Vol 21 (10) ◽  
pp. 3593 ◽  
Author(s):  
Konda Kumaraswami ◽  
Natallia Salei ◽  
Sebastian Beck ◽  
Stephan Rambichler ◽  
Anna-Kristina Kluever ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Arterial Disease ◽  
Tissue Samples ◽  
Collateral Arteries ◽  
Optimized Protocol ◽  
Adductor Muscles ◽  
Protocol Flow ◽  
Peripheral Arterial ◽  
Perivascular Infiltration ◽  
Detailed Protocol

Arteriogenesis, the growth of a natural bypass from pre-existing arteriolar collaterals, is an endogenous mechanism to compensate for the loss of an artery. Mechanistically, this process relies on a locally and temporally restricted perivascular infiltration of leukocyte subpopulations, which mediate arteriogenesis by supplying growth factors and cytokines. Currently, the state-of-the-art method to identify and quantify these leukocyte subpopulations in mouse models is immunohistology. However, this is a time consuming procedure. Here, we aimed to develop an optimized protocol to identify and quantify leukocyte subpopulations by means of flow cytometry in adductor muscles containing growing collateral arteries. For that purpose, adductor muscles of murine hindlimbs were isolated at day one and three after induction of arteriogenesis, enzymatically digested, and infiltrated leukocyte subpopulations were identified and quantified by flow cytometry, as exemplary shown for neutrophils and macrophages (defined as CD45+/CD11b+/Ly6G+ and CD45+/CD11b+/F4/80+ cells, respectively). In summary, we show that flow cytometry is a suitable method to identify and quantify leukocyte subpopulations in muscle tissue, and provide a detailed protocol. Flow cytometry constitutes a timesaving tool compared to histology, which might be used in addition for precise localization of leukocytes in tissue samples.

scholarly journals iGEM Calibration Protocol - Flow Cytometry Fluorescence v1 (protocols.io.2pcgdiw)

protocols.io ◽  
2019 ◽  
Author(s):  
Jacob Beal ◽  
Cheryl Telmer ◽  
Richard Tennant ◽  
Paul Rutten
Keyword(s):  
Flow Cytometry ◽  
Calibration Protocol ◽  
Protocol Flow

Flow cytometry using the monoclonal antibody CD10-Pe/Cy5 is a useful tool to identify follicular lymphoma cells

2001 ◽  
Vol 66 (2) ◽  
pp. 100-106 ◽  
Author(s):  
M. Bellido ◽  
E. Rubiol ◽  
J. Ubeda ◽  
O. Lopez ◽  
C. Estivill ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Flow Cytometry ◽  
Follicular Lymphoma ◽  
Lymphoma Cells
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