Gambierdiscus Whole Cell Hybridization v1

protocols.io ◽  
2015 ◽  
Author(s):  
Katie Pitz
Keyword(s):  
Cell Hybridization ◽  
Whole Cell

scholarly journals In situ analysis of the bacterial communities associated to farmed eel by whole-cell hybridization

1999 ◽  
Vol 29 (3) ◽  
pp. 160-165 ◽  
Author(s):  
Y. Moreno ◽  
C. R. Arias ◽  
H. Meier ◽  
E. Garay ◽  
R. Aznar
Keyword(s):  
Bacterial Communities ◽  
In Situ Analysis ◽  
Cell Hybridization ◽  
Whole Cell

Detection of mRNA of nprM in Bacillus megaterium ATCC 14581 grown in soil by whole-cell hybridization

1995 ◽  
Vol 163 (4) ◽  
pp. 235-241 ◽  
Author(s):  
Wolfgang H�nerlage ◽  
Dittmar Hahn ◽  
Josef Zeyer
Keyword(s):  
Bacillus Megaterium ◽  
Cell Hybridization ◽  
Whole Cell

scholarly journals Oligonucleotide Probes for the Identification of Three Algal Groups by Dot Blot and Fluorescent Whole-Cell Hybridization

2000 ◽  
Vol 47 (1) ◽  
pp. 76-84 ◽  
Author(s):  
NATHALIE SIMON ◽  
LISA CAMPBELL ◽  
ERLA ORNOLFSDOTTIR ◽  
RENE GROBEN ◽  
LAURE GUILLOU ◽  
...  
Keyword(s):  
Oligonucleotide Probes ◽  
Dot Blot ◽  
Cell Hybridization ◽  
Whole Cell ◽  
Algal Groups

scholarly journals Fluorescent Whole-Cell Hybridization with 16S rRNA-Targeted Oligonucleotide Probes To Identify Brucellaspp. by Flow Cytometry

2000 ◽  
Vol 38 (7) ◽  
pp. 2768-2771 ◽  
Author(s):  
Luis Fernández-Lago ◽  
F. Javier Vallejo ◽  
Ignacio Trujillano ◽  
Nieves Vizcaíno
Keyword(s):  
Flow Cytometry ◽  
16S Rrna ◽  
Clinical Isolates ◽  
Hybridization Assay ◽  
Hybridization Technique ◽  
Oligonucleotide Probes ◽  
Cell Hybridization ◽  
16S Rrna Sequence ◽  
Whole Cell ◽  
Detection And Identification

A whole-cell hybridization assay with fluorescent oligonucleotide probes derived from the 16S rRNA sequence of Brucella abortus in combination with flow cytometry has been developed. With the three fluorescent probes selected, a positive signal was observed with all the representative strains of the species and biovars of Brucella and with a total of nine differentBrucella clinical isolates. Using the B9 probe in the hybridization assay, it was possible to discriminate betweenBrucella suis biovars 2, 3, 4, and 5 and almost all the other Brucella spp. On the basis of differences in fluorescence intensities, no discrimination was established betweenBrucella spp. and other phylogenetically related microorganisms. No positive fluorescence signals were detected with any of the bacteria showing serological cross-reactions withBrucella spp. and with a total of 17 clinical isolates not belonging to the genus Brucella. These results suggest that the 16S rRNA whole-cell hybridization technique could be a valuable diagnostic tool for the detection and identification ofBrucella spp.

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