Flow Cytometry ICS Nuclear Antigens v1

Demonstration of cytoplasmic and nuclear antigens in acute leukaemia using flow cytometry.

Journal of Clinical Pathology ◽

10.1136/jcp.47.9.843 ◽

1994 ◽

Vol 47 (9) ◽

pp. 843-849 ◽

Cited By ~ 26

Author(s):

N Farahat ◽

D van der Plas ◽

M Praxedes ◽

R Morilla ◽

E Matutes ◽

...

Keyword(s):

Flow Cytometry ◽

Acute Leukaemia ◽

Nuclear Antigens

Download Full-text

A novel method of microwave treatment for detection of cytoplasmic and nuclear antigens by flow cytometry

Journal of Immunological Methods ◽

10.1016/0022-1759(95)00233-2 ◽

1996 ◽

Vol 190 (1) ◽

pp. 1-10 ◽

Cited By ~ 26

Author(s):

Hui Y. Lan ◽

Paul Hutchinson ◽

Gregory H. Tesch ◽

Wei Mu ◽

Robert C. Atkins

Keyword(s):

Flow Cytometry ◽

Microwave Treatment ◽

Nuclear Antigens ◽

Novel Method

Download Full-text

Limitations in the measurement ofc-myc oncoprotein and other nuclear antigens by flow cytometry

Cytometry ◽

10.1002/cyto.990100414 ◽

1989 ◽

Vol 10 (4) ◽

pp. 456-462 ◽

Cited By ~ 20

Author(s):

Sarah T. Lincoln ◽

Kenneth D. Bauer

Keyword(s):

Flow Cytometry ◽

Nuclear Antigens

Download Full-text

Flow Cytometry ICS Nuclear Antigens v2

protocols.io ◽

10.17504/protocols.io.bwuupeww ◽

2021 ◽

Author(s):

Michael Betts ◽

Gregory Golden

Keyword(s):

Flow Cytometry ◽

Nuclear Antigens

Download Full-text

Immunolocalization of nuclear antigens in cryofixed cells which have been prepared by molecular distillation drying or freeze-substitution

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100162053 ◽

1990 ◽

Vol 48 (3) ◽

pp. 898-899

Author(s):

David L. Spector ◽

Robert J. Derby

Keyword(s):

Cell Nucleus ◽

Molecular Distillation ◽

Functional Organization ◽

Freeze Substitution ◽

3 Dimensional ◽

Small Nuclear Ribonucleoprotein ◽

Nuclear Antigens ◽

Dimensional Reconstruction ◽

Nuclear Regions ◽

Immunoperoxidase Labeling

Studies in our laboratory are involved in evaluating the structural and functional organization of the mammalian cell nucleus. Since several major classes (U1, U2, U4/U6, U5) of small nuclear ribonucleoprotein particles (snRNPs) play a crucial role in the processing of pre-mRNA molecules, we have been interested in the localization of these particles within the cell nucleus. Using pre-embedding immunoperoxidase labeling combined with 3-dimensional reconstruction, we have recently shown that nuclear regions enriched in snRNPs form a reticular network within the nucleoplasm which extends between the nucleolar surface and the nuclear envelope. In the present study we were inte rested in extending these nuclear localizations using cell preparation techniques which avoid slow penetration of fixatives, chemical crosslinking of potential antigens and solvent extraction. CHOC 400 cells were cryofixed using a CF 100 ultra rapid cooling device (LifeCell Corp.). After cryofixation cells were molecular distillation dried, vapor osmicated, in filtra ted in 100% Spurr resin in vacuo and polymerized in molds a t 60°C. Using this procedure we were able to evaluate the distribution of snRNPs in resin embedded cells which had not been chemically fixed, incubated in cryoprotectants or extracted with solvents.

Download Full-text