scholarly journals Mutational Analysis of Hepatitis B Virus Precore Region Molecular Variants in Ile-ife, Nigeria v1 (protocols.io.bugwntxe)

protocols.io ◽  
2021 ◽  
Author(s):  
Oluwadamilola Osasona ◽  
Olumuyiwa Ariyo
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Mutational Analysis ◽  
Molecular Variants ◽  
Precore Region ◽  
B Virus

Fulminant hepatitis B: Induction by hepatitis B virus mutants defective in the precore region and incapable of encoding E antigen

1991 ◽  
Vol 100 (4) ◽  
pp. 1087-1094 ◽  
Author(s):  
Yoshitane Kosaka ◽  
Kohjiro Takase ◽  
Mineo Kojima ◽  
Masaru Shimizu ◽  
Kyoichi Inoue ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Fulminant Hepatitis ◽  
Precore Region ◽  
B Virus

scholarly journals N6-methyladenosine modification of hepatitis B virus RNA differentially regulates the viral life cycle

2018 ◽  
Vol 115 (35) ◽  
pp. 8829-8834 ◽  
Author(s):  
Hasan Imam ◽  
Mohsin Khan ◽  
Nandan S. Gokhale ◽  
Alexa B. R. McIntyre ◽  
Geon-Woo Kim ◽  
...  
Keyword(s):  
Life Cycle ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Reverse Transcription ◽  
Mutational Analysis ◽  
Regulatory Function ◽  
Messenger Rnas ◽  
Stem Loop ◽  
B Virus ◽  
A Site

N6-methyladenosine (m6A) RNA methylation is the most abundant epitranscriptomic modification of eukaryotic messenger RNAs (mRNAs). Previous reports have found m6A on both cellular and viral transcripts and defined its role in regulating numerous biological processes, including viral infection. Here, we show that m6A and its associated machinery regulate the life cycle of hepatitis B virus (HBV). HBV is a DNA virus that completes its life cycle via an RNA intermediate, termed pregenomic RNA (pgRNA). Silencing of enzymes that catalyze the addition of m6A to RNA resulted in increased HBV protein expression, but overall reduced reverse transcription of the pgRNA. We mapped the m6A site in the HBV RNA and found that a conserved m6A consensus motif situated within the epsilon stem loop structure, is the site for m6A modification. The epsilon stem loop is located in the 3′ terminus of all HBV mRNAs and at both the 5′ and 3′ termini of the pgRNA. Mutational analysis of the identified m6A site in the 5′ epsilon stem loop of pgRNA revealed that m6A at this site is required for efficient reverse transcription of pgRNA, while m6A methylation of the 3′ epsilon stem loop results in destabilization of all HBV transcripts, suggesting that m6A has dual regulatory function for HBV RNA. Overall, this study reveals molecular insights into how m6A regulates HBV gene expression and reverse transcription, leading to an increased level of understanding of the HBV life cycle.

scholarly journals Complete Genome Sequence of a Precore-Defective Hepatitis B Virus Genotype D2 Strain Isolated in Bangladesh

2020 ◽  
Vol 9 (11) ◽  
Author(s):  
Md Golzar Hossain ◽  
Md Muket Mahmud ◽  
Md Arifur Rahman ◽  
Sharmin Akter ◽  
K. H. M. Nazmul Hussain Nazir ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Nucleotide Sequence ◽  
Hepatitis B ◽  
Complete Genome Sequence ◽  
Complete Nucleotide Sequence ◽  
Mutational Analysis ◽  
Limited Information ◽  
Virus Genotype ◽  
Hbv Genotype ◽  
B Virus

Hepatitis B virus (HBV) genomic mutations affect viral replication, disease progression, and diagnostic and vaccination efficiency. There is limited information regarding characterization and mutational analysis of HBV isolated in Bangladesh. Here, we report the complete nucleotide sequence of a precore-defective HBV genotype D2 strain isolated in Bangladesh.

scholarly journals Hepatitis B Virus Mutants with Precore-Region Defects in Two Babies with Fulminant Hepatitis and Their Mothers Positive for Antibody to Hepatitis B e Antigen

1991 ◽  
Vol 29 (1) ◽  
pp. 5-9 ◽  
Author(s):  
Sousuke Terazawa ◽  
Mineo Kojima ◽  
Tatsuru Yamanaka ◽  
Shigeru Yotsumoto ◽  
Hiroaki Okamoto ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Fulminant Hepatitis ◽  
Hepatitis B E Antigen ◽  
Precore Region ◽  
B Virus

Mutations in the Precore Region of Hepatitis B Virus DNA in Patients with Fulminant and Severe Hepatitis

1991 ◽  
Vol 324 (24) ◽  
pp. 1699-1704 ◽  
Author(s):  
Masao Omata ◽  
Toshiki Ehata ◽  
Osamu Yokosuka ◽  
Kazuhiko Hosoda ◽  
Masao Ohto
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Severe Hepatitis ◽  
Hepatitis B Virus Dna ◽  
Precore Region ◽  
B Virus

scholarly journals Mutations in the basic core promotor and the precore region of hepatitis b virus and their selection in children with fulminant and chronic hepatitis B

Hepatology ◽  
1999 ◽  
Vol 29 (4) ◽  
pp. 1252-1258 ◽  
Author(s):  
Michael Friedt ◽  
Patrick Gerner ◽  
Ekkehart Lausch ◽  
Hubert Trübel ◽  
Bernhard Zabel ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Chronic Hepatitis B ◽  
Precore Region ◽  
B Virus

scholarly journals Hepatitis B Virus of Genotype B with or without Recombination with Genotype C over the Precore Region plus the Core Gene

2002 ◽  
Vol 76 (12) ◽  
pp. 5985-5992 ◽  
Author(s):  
Fuminaka Sugauchi ◽  
Etsuro Orito ◽  
Takafumi Ichida ◽  
Hideaki Kato ◽  
Hiroshi Sakugawa ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Core Gene ◽  
Entire Genome ◽  
Amino Acid Homology ◽  
The Core ◽  
Dna Database ◽  
Precore Region ◽  
B Virus ◽  
Genotype C

ABSTRACT The entire nucleotide sequences of 70 hepatitis B virus (HBV) isolates of genotype B (HBV/B), including 38 newly determined and 32 retrieved from the international DNA database (DDBJ/EMBL/GenBank), were compared phylogenetically. Two subgroups of HBV/B were identified based on sequence divergence in the precore region plus the core gene, one with the recombination with genotype C and the other without it. The analysis over the entire genome of HBV/B by the SimPlot program located the recombination with genotype C in the precore region plus the core gene spanning nucleotide positions from 1740 to 1838 to 2443 to 2485. Within this genomic area, HBV/B strains with the recombination had higher nucleotide and amino acid homology to genotype C than those without the recombination (96.9 versus 91.1% in nucleotides and 97.0 versus 92.9% in amino acids). There were 29 HBV/B strains without the recombination, and they were all recovered from carriers in Japan. The remaining 41 HBV/B isolates having the recombination with genotype C were from carriers in China (12 strains), Hong Kong (3 strains), Indonesia (4 strains), Japan (3 strains), Taiwan (4 strains), Thailand (3 strains), and Vietnam (12 strains). Due to the frequency of the distribution of HBV/B without the recombination (29 of 32 isolates, or 91%) and the fact that it was exclusive to Japan, it was provisionally classified into the Bj (j standing for Japan) subgroup, and HBV/B with the recombination was classified into the Ba (a for Asia) subgroup. Virological differences between HBV/Bj and HBV/Ba may be reflected in the severity of clinical disease in the patients infected with HBV of genotype B, which seems to be under strong geographic influences in Asia.

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