scholarly journals Maize hydroponics & Root multiple ion uptake kinetics [sterilization, germination, sowing, growth & uptake] v1 (protocols.io.bqfmmtk6)

protocols.io ◽  
2020 ◽  
Author(s):  
Marcus Griffiths ◽  
Sonali Roy ◽  
Haichao Guo ◽  
Anand Seethepalli ◽  
David Huhman ◽  
...  
Keyword(s):  
Uptake Kinetics ◽  
Ion Uptake

scholarly journals A multiple ion-uptake phenotyping platform reveals shared mechanisms that affect nutrient uptake by maize roots

2020 ◽  
Author(s):  
Marcus Griffiths ◽  
Sonali Roy ◽  
Haichao Guo ◽  
Anand Seethepalli ◽  
David Huhman ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Nutrient Uptake ◽  
High Throughput ◽  
Root Respiration ◽  
Uptake Kinetics ◽  
Ion Uptake ◽  
Regulatory Pathways ◽  
Uptake Rates ◽  
High Throughput Phenotyping ◽  
Specific Uptake

Nutrient uptake is critical for crop growth and determined by root foraging in soil. Growth and branching of roots lead to effective root placement to acquire nutrients, but relatively less is known about absorption of nutrients at the root surface from the soil solution. This knowledge gap could be alleviated by understanding sources of genetic variation for short-term nutrient uptake on a root length basis. A new modular platform for high-throughput phenotyping of multiple ion uptake kinetics was designed to determine nutrient uptake rates in Zea mays. Using this system, uptake rates were characterized for the crop macronutrients nitrate, ammonium, potassium, phosphate and sulfate among the Nested Association Mapping (NAM) population founder lines. The data revealed that substantial genetic variation exists for multiple ion uptake rates in maize. Interestingly, specific nutrient uptake rates (nutrient uptake rate per length of root) were found to be both heritable and distinct from total uptake and plant size. The specific uptake rates of each nutrient were positively correlated with one another and with specific root respiration (root respiration rate per length of root), indicating that uptake is governed by shared mechanisms. We selected maize lines with high and low specific uptake rates and performed an RNA-seq analysis, which identified key regulatory components involved in nutrient uptake. The high-throughput multiple ion uptake kinetics pipeline will help further our understanding of nutrient uptake, parameterize holistic plant models, and identify breeding targets for crops with more efficient nutrient acquisition.Significance StatementNutrient uptake is among the most limiting factors for plant growth and yet has not been used as a selection criterion in breeding. This is partly due to the lack of high-throughput phenotyping methods for measuring nutrient uptake. Here we describe a novel high-throughput phenotyping pipeline for quantification of multiple ion uptake rates. Using this new phenotyping system, our results demonstrate that specific ion uptake performance by maize plants is positively correlated among the macronutrients nitrogen, phosphorus, potassium and sulfur, and that substantial variation exists within a genetically diverse population. The findings reveal components of regulatory pathways possibly related with enhanced uptake, and confirm that nutrient uptake itself is a potential target for breeding of nutrient-efficient crops.

Aqueous “polysulfide-ene” polymerization for sulfur-rich nanoparticles and their use in heavy metal ion remediation

2018 ◽  
Vol 6 (46) ◽  
pp. 23542-23549 ◽  
Author(s):  
Hyuksoo Shin ◽  
Jihee Kim ◽  
Dowan Kim ◽  
Viet Huu Nguyen ◽  
Sangwook Lee ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Ion ◽  
Mercury Ion ◽  
Uptake Kinetics ◽  
Ion Uptake ◽  
Polymer Nanoparticles ◽  
Heavy Metal Ion ◽  
High Mercury ◽  
Very High ◽  
Capture Capacity

Thiol-functionalized polymer nanoparticles demonstrated well-defined mercury ion uptake kinetics, along with a very high mercury ion capture capacity.

Sodium bicarbonate ingestion does not alter the slow component of oxygen uptake kinetics in professional cyclists

2003 ◽  
Vol 21 (1) ◽  
pp. 39-47 ◽  
Author(s):  
ALFREDO SANTALLA ◽  
MARGARITA PÉREZ ◽  
MANUEL MONTILLA ◽  
LÁZARO VICENTE ◽  
RICHARD DAVISON ◽  
...  
Keyword(s):  
Oxygen Uptake ◽  
Sodium Bicarbonate ◽  
Slow Component ◽  
Oxygen Uptake Kinetics ◽  
Uptake Kinetics

Comparisons of NH4+, NO3- and H2PO4- uptake kinetics in three different macrophytes in waterlogged condition

2011 ◽  
Vol 19 (3) ◽  
pp. 614-618 ◽  
Author(s):  
Yi-Xuan TANG ◽  
Jie-Min ZHENG ◽  
Li-Ping LOU ◽  
Qi-Chun ZHANG
Keyword(s):  
Uptake Kinetics ◽  
Waterlogged Condition

Uptake kinetics and utilization characteristics of Prorocentrum donghaiense for urea

2012 ◽  
Vol 29 (5) ◽  
pp. 455-460 ◽  
Author(s):  
Ning XU ◽  
Jun-lian QIN ◽  
Shu-gang SUN ◽  
Shun-shan DUAN
Keyword(s):  
Uptake Kinetics ◽  
Prorocentrum Donghaiense
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