Low cost, versatile, and rapid antimicrobial susceptibility testing using gel-modified screen-printed electrodes v1 (protocols.io.bng3mbyn)

protocols.io ◽  
2020 ◽  
Author(s):  
Stuart Hannah ◽  
Perrine Lasserre ◽  
Alexandra Dobrea ◽  
Ewen O ◽  
David Alcorn ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Low Cost ◽  
Antimicrobial Susceptibility Testing ◽  
Screen Printed Electrodes ◽  
Screen Printed

scholarly journals Rapid antibiotic susceptibility testing using low-cost, commercially available screen-printed electrodes

2019 ◽  
Vol 145 ◽  
pp. 111696 ◽  
Author(s):  
Stuart Hannah ◽  
Emily Addington ◽  
David Alcorn ◽  
Wenmiao Shu ◽  
Paul A. Hoskisson ◽  
...  
Keyword(s):  
Antibiotic Susceptibility ◽  
Susceptibility Testing ◽  
Low Cost ◽  
Antibiotic Susceptibility Testing ◽  
Screen Printed Electrodes ◽  
Screen Printed

scholarly journals Development of a Rapid, Antimicrobial Susceptibility Test for E. coli Based on Low-Cost, Screen-Printed Electrodes

Biosensors ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 153 ◽  
Author(s):  
Stuart Hannah ◽  
Alexandra Dobrea ◽  
Perrine Lasserre ◽  
Ewen O. Blair ◽  
David Alcorn ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Low Cost ◽  
Diagnostic Testing ◽  
World Health ◽  
E Coli ◽  
Growth Profiles ◽  
Tract Infections ◽  
Screen Printed

Antibiotic resistance has been cited by the World Health Organisation (WHO) as one of the greatest threats to public health. Mitigating the spread of antibiotic resistance requires a multipronged approach with possible interventions including faster diagnostic testing and enhanced antibiotic stewardship. This study employs a low-cost diagnostic sensor test to rapidly pinpoint the correct antibiotic for treatment of infection. The sensor comprises a screen-printed gold electrode, modified with an antibiotic-seeded hydrogel to monitor bacterial growth. Electrochemical growth profiles of the common microorganism, Escherichia coli (E. coli) (ATCC 25922) were measured in the presence and absence of the antibiotic streptomycin. Results show a clear distinction between the E. coli growth profiles depending on whether streptomycin is present, in a timeframe of ≈2.5 h (p < 0.05), significantly quicker than the current gold standard of culture-based antimicrobial susceptibility testing. These results demonstrate a clear pathway to a low cost, phenotypic and reproducible antibiotic susceptibility testing technology for the rapid detection of E. coli within clinically relevant concentration ranges for conditions such as urinary tract infections.

Pretreatment antimicrobial susceptibility testing is cost saving in the eradication of

2003 ◽  
Vol 1 (4) ◽  
pp. 273-278 ◽  
Author(s):  
Marco Romano ◽  
Riccardo Marmo ◽  
Antonio Cuomo ◽  
Teresa De Simone ◽  
Caterina Mucherino ◽  
...  
Keyword(s):  
Cost Saving ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing

NGHIÊN CỨU SỰ NHẠY CẢM VỚI KHÁNG SINH CỦA MỘT SỐ CHỦNG VI KHUẨN GÂY VIÊM ĐƯỜNG HÔ HẤP CẤP Ở TRẺ EM DƯỚI 6 TUỔI TẠI BỆNH VIỆN NHI THANH HÓA 2009 - 2014

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Hoai Do Ngoc
Keyword(s):  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Antimicrobial Susceptibility Testing ◽  
High Rate ◽  
Isolation Rate

From 43.574 fluid nasopharynx speciments of  the chidren inpatient under six we isolated total 21.769 types bacteria with isolation rate : 49.95%. In which the highest isolation rate for H. influenza, S. pneumoniae and M. catarrhalis were 13,94%; 7,11%; 1,43% respectively. Antimicrobial susceptibility testing shown all the types of  for H. influenza, S. pneumoniae and M. catarrhalis good susses to Fosphomycine, S. pneumoniae and M. catarrhalis good susses to Imipenem, H. influenza good susses to Azithromycine, S. pneumoniae good susses to Penicilline and Piperacilline, M. catarrhalis good susses to Tobramycine and Ofloxacine. All of  H. influenza, S. pneumoniae and M. catarrhalis were reported resistance to Tri/Sulpha, Chloramphenicol, Erythromycine in high rate.

scholarly journals Specific and rapid reverse assaying protocol for detection and antimicrobial susceptibility testing of Pseudomonas aeruginosa based on dual molecular recognition

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yong He ◽  
Hang Zhao ◽  
Yuanwen Liu ◽  
He Zhou
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Susceptibility ◽  
Magnetic Particles ◽  
Susceptibility Testing ◽  
Fluorescein Isothiocyanate ◽  
High Specificity ◽  
Antimicrobial Susceptibility Testing ◽  
Tail Fiber ◽  
Isolation And Identification ◽  
Magainin Ii

AbstractThe worldwide emergence and spread of antimicrobial resistance is accelerated by irrational administration and use of empiric antibiotics. A key point to the crisis is a lack of rapid diagnostic protocols for antimicrobial susceptibility testing (AST), which is crucial for a timely and rational antibiotic prescription. Here, a recombinant bacteriophage tail fiber protein (TFP) was functionalized on magnetic particles to specifically capture Pseudomonas aeruginosa, while fluorescein isothiocyanate-labeled-magainin II was utilized as the indicator. For solving the magnetic particles’ blocking effects, a reverse assaying protocol based on TFP recognition was developed to investigate the feasibility of detection and AST of P. aeruginosa. P. aeruginosa can be rapidly, sensitively and specifically detected within 1.5 h with a linear range of 1.0 × 102 to 1.0 × 106 colony forming units (CFU)⋅mL−1 and a detection limit of 3.3 × 10 CFU⋅mL−1. Subsequently, AST results, which were consistent with broth dilution results, can be obtained within 3.5 h. Due to the high specificity of the TFP, AST can actually be conducted without the need for bacterial isolation and identification. Based on the proof-of-principle work, the detection and AST of other pathogens can be extended by expressing the TFPs of their bacteriophages.

Sign in / Sign up

Export Citation Format

Share Document