scholarly journals Slice Preparation and Blockface Imaging for Electrophysiology - Mouse v4 (protocols.io.be2gjgbw)

protocols.io ◽  
2020 ◽  
Author(s):  
Allen Institute
Keyword(s):  
Slice Preparation

AMPA Receptor Blockade Potentiates the Stimulatory Effect of L-DOPA on Dopamine Release in Dopamine-Deficient Corticostriatal Slice Preparation

2004 ◽  
Vol 16 (1-2) ◽  
pp. 129-139 ◽  
Author(s):  
Zsolt Juranyi ◽  
Nora Sziray ◽  
Bernadett Marko ◽  
Gyorgy Levay ◽  
Laszlo G. Harsing, Jr.
Keyword(s):  
Ampa Receptor ◽  
Dopamine Release ◽  
Receptor Blockade ◽  
Slice Preparation

23. A new adult rat brain slice preparation for ex vivo NMR spectroscopy studies of stroke

1994 ◽  
Vol 52 (1) ◽  
pp. A11
Author(s):  
M.T. Espanol ◽  
L. Litt ◽  
L.-H. Chang ◽  
T.L. James ◽  
P.R. Weinstein ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Rat Brain ◽  
Brain Slice ◽  
Ex Vivo ◽  
Slice Preparation ◽  
Adult Rat ◽  
Adult Rat Brain ◽  
Spectroscopy Studies ◽  
Rat Brain Slice ◽  
Brain Slice Preparation

Folic acid has a disinhibitory action in the rat hippocampal slice preparation

1985 ◽  
Vol 346 (2) ◽  
pp. 281-286 ◽  
Author(s):  
Laura C. Otis ◽  
Daniel V. Madison ◽  
Roger A. Nicoll
Keyword(s):  
Folic Acid ◽  
Hippocampal Slice ◽  
Slice Preparation

Size Does Matter: Generation of Intrinsic Network Rhythms in Thick Mouse Hippocampal Slices

2005 ◽  
Vol 93 (4) ◽  
pp. 2302-2317 ◽  
Author(s):  
Chiping Wu ◽  
Wah Ping Luk ◽  
Jesse Gillis ◽  
Frances Skinner ◽  
Liang Zhang
Keyword(s):  
In Vitro Model ◽  
Adult Mouse ◽  
Hippocampal Slices ◽  
Network Connectivity ◽  
Slice Preparation ◽  
Fundamental Properties ◽  
Mouse Hippocampus ◽  
Hippocampal Network ◽  
Vitro Model

Rodent hippocampal slices of ≤0.5 mm thickness have been widely used as a convenient in vitro model since the 1970s. However, spontaneous population rhythmic activities do not consistently occur in this preparation due to limited network connectivity. To overcome this limitation, we develop a novel slice preparation of 1 mm thickness from adult mouse hippocampus by separating dentate gyrus from CA3/CA1 areas but preserving dentate–CA3-CA1 connectivity. While superfused in vitro at 32 or 37°C, the thick slice exhibits robust spontaneous network rhythms of 1–4 Hz that originate from the CA3 area. Via assessing tissue O2, K+, pH, synaptic, and single-cell activities of superfused thick slices, we verify that these spontaneous rhythms are not a consequence of hypoxia and nonspecific experimental artifacts. We suggest that the thick slice contains a unitary circuitry sufficient to generate intrinsic hippocampal network rhythms and this preparation is suitable for exploring the fundamental properties and plasticity of a functionally defined hippocampal “lamella” in vitro.

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