Intracardiac perfusion with fixative for ultrastructural neuroanatomical studies v1

2021 ◽  
Author(s):  
Janet R Keast ◽  
Peregrine Osborne
Keyword(s):  
Spinal Cord ◽  
Peripheral Nerves ◽  
Animal Experimentation ◽  
Adult Rats

This protocol is suitable for preserving tissues for ultrastructural neuroanatomical studies of peripheral nerves, ganglia, spinal cord or brain in adult rats. The protocol is performed under anesthesia and should incorporate all local requirements for standards of animal experimentation.

Electrical Stimulation of the Spinal Cord and Peripheral Nerves for Pain Control

1981 ◽  
Vol 44 (4) ◽  
pp. 207-217 ◽  
Author(s):  
Don M. Long ◽  
Donald Erickson ◽  
James Campbell ◽  
Richard North
Keyword(s):  
Spinal Cord ◽  
Electrical Stimulation ◽  
Peripheral Nerves ◽  
Pain Control ◽  
Stimulation Of

Support of axonal regrowth by endogenous mechanisms following spinal cord injury in adult rats

2001 ◽  
Vol 21 (3) ◽  
pp. 188-202 ◽  
Author(s):  
Norman R West ◽  
Victoria LeBlanc ◽  
George H Collins
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Adult Rats ◽  
Axonal Regrowth ◽  
Cord Injury

Serotonin2 receptors mediate respiratory recovery after cervical spinal cord hemisection in adult rats

2001 ◽  
Vol 91 (6) ◽  
pp. 2665-2673 ◽  
Author(s):  
Shi-Yi Zhou ◽  
Gregory J. Basura ◽  
Harry G. Goshgarian
Keyword(s):  
Spinal Cord ◽  
Receptor Antagonist ◽  
Phrenic Nerve ◽  
Cervical Spinal Cord ◽  
Sprague Dawley ◽  
Related Activity ◽  
Adult Rats ◽  
Cord Injury ◽  
Respiratory Recovery ◽  
Spinal Cord Hemisection

The aim of the present study was to specifically investigate the involvement of serotonin [5-hydroxytryptamine (5-HT2)] receptors in 5-HT-mediated respiratory recovery after cervical hemisection. Experiments were conducted on C2 spinal cord-hemisected, anesthetized (chloral hydrate, 400 mg/kg ip), vagotomized, pancuronium- paralyzed, and artificially ventilated female Sprague-Dawley rats in which CO2 levels were monitored and maintained. Twenty-four hours after spinal hemisection, the ipsilateral phrenic nerve displayed no respiratory-related activity indicative of a functionally complete hemisection. Intravenous administration of the 5-HT2A/2C-receptor agonist (±)-2,5-dimethoxy-4-iodoamphetamine hydrochloride (DOI) induced respiratory-related activity in the phrenic nerve ipsilateral to hemisection under conditions in which CO2 was maintained at constant levels and augmented the activity induced under conditions of hypercapnia. The effects of DOI were found to be dose dependent, and the recovery of activity could be maintained for up to 2 h after a single injection. DOI-induced recovery was attenuated by the 5-HT2-receptor antagonist ketanserin but not with the 5-HT2C-receptor antagonist RS-102221, suggesting that 5-HT2A and not necessarily 5-HT2C receptors may be involved in the induction of respiratory recovery after cervical spinal cord injury.

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