Catalase test for bacterial identification v1

2021 ◽  
Author(s):  
lydiariver not provided
Keyword(s):  
Hydrogen Peroxide ◽  
Bubble Formation ◽  
Bacterial Identification ◽  
Rapid Decomposition ◽  
Group B

Catalase is an enzyme that catalyzes the rapid decomposition of hydrogen peroxide into water and oxygen (2H2O2 + Catalase → 2H2O + O2). Organisms that do not present the catalase enzyme such as group B Streptoccoccus can not degrade hydrogen peroxide and therefore no bubble formation is observed.

scholarly journals Management of walled-off necrosis with nasocystic irrigation with hydrogen peroxide versus biflanged metal stent: randomized controlled trial

2021 ◽  
Vol 09 (07) ◽  
pp. E1108-E1115
Author(s):  
Sudhir Maharshi ◽  
Shyam Sunder Sharma ◽  
Sandeep Ratra ◽  
Bharat Sapra ◽  
Dhruv Sharma
Keyword(s):  
Hydrogen Peroxide ◽  
Adverse Events ◽  
Clinical Success ◽  
Controlled Trial ◽  
Procedure Time ◽  
Metal Stent ◽  
Technical Success ◽  
Group A ◽  
Group B ◽  
Walled Off Necrosis

Abstract Background and study aims Walled-off necrosis (WON) is a known complication of acute necrotizing pancreatitis (ANP). There is no study comparing nasocystic irrigation with hydrogen peroxide (H2O2) versus biflanged metal stent (BMS) in the management of WON. The aim of this study was to compare the clinical efficacy of both the treatment strategies. Patients and methods This study was conducted on patients with symptomatic WON who were randomized to nasocystic irrigation with H2O2 (Group A) and BMS placement (Group B). Primary outcomes were clinical and technical success while secondary outcomes were procedure time, adverse events, need for additional procedures, duration of hospitalization, and mortality. Results Fifty patients were randomized into two groups. Group A (n = 25, age 37.8 ± 17.6 years, 16 men) and Group B (n = 25, age 41.8 ± 15.2 years, 17 men). There were no significant differences in baseline characteristics between the two groups. The most common etiology of pancreatitis was alcohol, observed in 27 (54 %) patients. Technical success (100 % vs 96 %, P = 0.98), clinical success (84 % vs 76 %, P = 0.76), requirement of additional procedures (16 % vs 24 %, P = 0.70) and adverse events (4 vs 7, P = 0.06) were comparable in both the groups. The duration to clinical success (34.4 ± 12 vs 14.8 ± 10.8 days, P = 0.001) and procedure time (36 ± 15 vs 18 ± 12 minutes, P = 0.01) were longer in Group A compared to Group B. Conclusions Nasocystic irrigation with H2O2 and BMS are equally effective in the management of WON but time to clinical success and procedure time is longer with nasocystic irrigation.

The Role of HO2− in SC-1 Cleaning Solutions

1997 ◽  
Vol 477 ◽  
Author(s):  
Steven Verhaverbeke ◽  
Jennifer W. Parker ◽  
Chris F. McConnell
Keyword(s):  
Hydrogen Peroxide ◽  
Silicon Oxide ◽  
Bubble Formation ◽  
Ammonium Hydroxide ◽  
Silicon Surfaces ◽  
Wafer Surface ◽  
Particle Removal ◽  
Oxide Interface ◽  
Protective Oxide ◽  
Industry Standard

The RCA Standard Clean, developed by W. Kern and D. Puotinen in 1965 and disclosed in 1970 [1] is extremely effective at removing contamination from silicon surfaces and is the defacto industry standard.[2]. The RCA clean consists of two sequential steps: the Standard Clean 1 (SC-1) followed by the Standard Clean 2 (SC-2). The SC-1 solution, consisting of a mixture of ammonium-hydroxide, hydrogen-peroxide, and water, is the most efficient particle removing agent found to date. This mixture is also referred to as the Ammonium- Hydroxide/Hydrogen-Peroxide Mixture (APM). In the past, SC-1 solutions had the tendency to deposit metals on the surface of the wafers, and consequently treatment with the SC-2 mixture was necessary to remove metals. Ultra-clean chemicals minimize the need for SC-2 processing. SC-I solutions facilitate particle removal by etching the wafer underneath the particles; thereby loosening the particles, so that mechanical forces can readily remove the particles from the wafer surface. The ammonium hydroxide in the solution steadily etches silicon dioxide at the boundary between the oxide and the aqueous solution (i.e., the wafer surface). The hydrogen peroxide in SC-I serves to protect the surface from attack by OH" by re-growing a protective oxide directly on the silicon surface (i.e., at the silicon/oxide interface). If sufficient hydrogen peroxide is not present in the solution, the silicon will be aniostropically etched and surface roughening will quickly occur. On the other hand, hydrogen peroxide readily dissociates and forms water and oxygen. If the concentration of the resulting oxygen is too high, bubbles will appear in the solution. The gas liquid interfaces that result from the bubble formation act as a “getter” for particles that can re-deposit on the wafer surface if a bubble comes in contact with the wafer.

scholarly journals The Effect of Honey in Second Degree Burn Healing on Wistar Rats (Overview of Angiogenesis and the Number of Fibroblasts)

Sains Medika ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 27
Author(s):  
Ayyasi Izaz Almaz ◽  
Ratna Dama Purnawati ◽  
Hermawan Istiadi ◽  
Neni Susilaningsih
Keyword(s):  
Hydrogen Peroxide ◽  
Alternative Treatment ◽  
Wistar Rats ◽  
Vegf Expression ◽  
Group Design ◽  
Degree Burn ◽  
Group B ◽  
Post Hoc ◽  
Observation Results ◽  
Second Degree

Introduction: Burn therapy is generally done using a burn ointment. However, honey can also be used as an alternative treatment for burns. Honey contains active flavonoid as an antioxidant and hydrogen peroxide which can stimulate fibroblasts proliferation also angiogenesis on burn healing phase.Objective: to determine the effect of honey in second degree burn healing from angiogenesis and the number of fibroblasts on Wistar rats. Methods: This research used a posttest only controlled group design on 15 rats with random selection and divided into 3 groups: H-G was given honey, B-G was given Bioplacenton�, N-G without any treatment after burn was induced. Rats were given a second degree burn in a size of 2 cm x 2cm on the back area and were treated twice a day for 14 days. Burn healing was measured by calculating the observation results of microscopic VEGF expression, the number of neovascularization, and the number of fibroblasts. Hypothesis analysis used Annova post hoc LSD and Mann-Whitney.Results: The highest mean in VEGF expression and the number of neovascularization was obtained from group B-G followed by group H-G and group N-G. Significant differences in the number of neovascularization between group B-G with group N-G, p<0.05.Conclusion: Honey can provide a healing effect on the second degree of burns in Wistar rats in terms of the number of neovascularization as well as Bioplacenton�

scholarly journals Development of a Novel Test for Simultaneous Bacterial Identification and Antibiotic Susceptibility

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Jonathan Faro ◽  
Malika Mitchell ◽  
Yuh-Jue Chen ◽  
Sarah Kamal ◽  
Gerald Riddle ◽  
...  
Keyword(s):  
Incubation Period ◽  
Antimicrobial Susceptibility ◽  
Bacterial Resistance ◽  
Group B Streptococcus ◽  
Bacterial Identification ◽  
Specific Identification ◽  
Highly Sensitive ◽  
Group B ◽  
Reduced Time

Background. Elucidation of a pathogen’s antimicrobial susceptibility requires subculture after the organism is first isolated. This takes several days, requiring patients to be treated with broad-spectrum antibiotics. This approach contributes to the development of bacterial resistance.Methods. Microtiter wells were coated with a polyclonal antibody targeting the pathogen of interest. Bacterial suspensions were added in the presence/absence of selected antibiotics. After washing, captured bacteria were detected.Findings. Group B streptococcus (GBS),Enterococcus faecalis, andNeisseria gonorrhoeaewere each detected at 105 bacteria/mL following a 20-minute incubation period. Susceptibility to select antibiotics was discernable following a 6-hour incubation period (GBS andEnterococcus). Sensitivity was increased to 10−2 bacteria/mL for GBS, 10−1 bacteria/mL forE. faecalis, and 101 bacteria/mL forN. gonorrhoeaefollowing 18–24-hour culture.Conclusion. This novel assay allows for the highly sensitive and specific identification of a pathogen and simultaneous determination of its antimicrobial susceptibility in a reduced time.

Oxidative stress response of Mycosphaerella fijiensis, the causal agent of black leaf streak disease in banana plants, to hydrogen peroxide and paraquat

2009 ◽  
Vol 55 (7) ◽  
pp. 887-894 ◽  
Author(s):  
Miguel J. Beltrán-García ◽  
Gilberto Manzo-Sanchez ◽  
Salvador Guzmán-González ◽  
Carlos Arias-Castro ◽  
Martha Rodríguez-Mendiola ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Stress Responses ◽  
Antioxidant Response ◽  
Mycosphaerella Fijiensis ◽  
Native Page ◽  
Rapid Decomposition ◽  
Black Leaf Streak ◽  
Catalase Peroxidase ◽  
Black Leaf Streak Disease

Mycosphaerella fijiensis causes black leaf streak disease in banana and plantain. This fungus is usually attacked by reactive oxygen species secreted by the plant or during exposure to fungicide, however, little is known about the antioxidant response of the fungus. In this study, mycelia were observed to totally decompose 30 mmol/L of hydrogen peroxide (H2O2) within 120 min, liberating oxygen bubbles, and also to survive in concentrations as high as 100 mmol/L H2O2. The oxidative stress responses to H2O2, paraquat, and hydroquinone were characterized in terms of the activities of catalase and superoxide dismutase (SOD). Two active catalase bands were seen in native PAGE induced by H2O2. Band I had monofunctional activity and band II had bifunctional catalase–peroxidase activity. Two isozymes of SOD, distinguishable by their cyanide sensitivity, were found; CuZnSOD was the main one. The combination of H2O2 and 3-aminotriazole reduced the accumulation of biomass up to 40% compared with exposure to H2O2 alone, suggesting that catalase is important for the rapid decomposition of H2O2 and has a direct bearing on cell viability. The results also suggest that the superoxide anion formed through the redox of paraquat and hydroquinone has a greater effect than H2O2 on the cellular viability of M. fijiensis.

Rapid decomposition of chloroform by a liquid phase plasma reaction with titanium dioxide and hydrogen peroxide

2020 ◽  
Vol 352 ◽  
pp. 54-59
Author(s):  
Sang-Chul Jung ◽  
Heon Lee ◽  
Seo Jin Ki ◽  
Sun-Jae Kim ◽  
Young-Kwon Park
Keyword(s):  
Hydrogen Peroxide ◽  
Titanium Dioxide ◽  
Liquid Phase ◽  
Rapid Decomposition ◽  
Plasma Reaction ◽  
Liquid Phase Plasma

Comparison of Effectiveness and Sensitivity Using Two In-Office Bleaching Protocols for a 6% Hydrogen Peroxide Gel in a Randomized Clinical Trial

2017 ◽  
Vol 42 (3) ◽  
pp. 244-252 ◽  
Author(s):  
P Vildósola ◽  
F Vera ◽  
J Ramírez ◽  
J Rencoret ◽  
H Pretel ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Hydrogen Peroxide ◽  
Randomized Clinical Trial ◽  
Absolute Risk ◽  
Color Variation ◽  
Tooth Sensitivity ◽  
Tooth Color ◽  
Protocol Group ◽  
Group A ◽  
Group B

SUMMARY Objective: The aim of this blinded and randomized clinical trial was to compare two application protocols (one 36-minute application vs three 12-minute applications). We then assessed the effectiveness of the bleaching and any increase in sensitivity that was induced by bleaching via a split-mouth design. Methods and Materials: Thirty patients were treated. One group had a half arch of teeth treated with a traditional application protocol (group A: 3 × 12 minutes for two sessions). The other received an abbreviated protocol (group B: 1 × 36 minutes over two sessions). Two sessions were appointed with a two-day interval between them. The tooth color was registered at each session, as well as one week and one month after completing the treatment via a spectrophotometer. This measured L*, a*, and b*. This was also evaluated subjectively using the VITA classical A1-D4 guide and VITA Bleachedguide 3D-MASTER. Tooth sensitivity was registered according to the visual analogue scale (VAS) scale. Tooth color variation and sensitivity were compared between groups. Results: Both treatments changed tooth color vs baseline. The ΔE* = 5.71 ± 2.62 in group A, and ΔE* = 4.93 ± 2.09 in group B one month after completing the bleaching (p=0.20). No statistical differences were seen via subjective evaluations. There were no differences in tooth sensitivity between the groups. The absolute risk of sensitivity reported for both groups was 6.25% (p=0.298). The intensity by VAS was mild (p=1.00). Conclusions: We used hydrogen peroxide (6%) that was light activated with a hybrid LED/laser and two different protocols (one 36-minute application vs three 12-minute applications each for two sessions). These approaches were equally effective. There were no differences in absolute risk of sensitivity; both groups reported mild sensitivity.

scholarly journals Comparative Study of Two Different Techniques for Management of Dry Socket

2015 ◽  
Vol 12 (1) ◽  
pp. 6-9
Author(s):  
N Kansakar ◽  
H Mahendra ◽  
S Acharya
Keyword(s):  
Hydrogen Peroxide ◽  
Contraceptive Use ◽  
Blood Clot ◽  
Gingival Tissue ◽  
Duration Of Treatment ◽  
Medical College ◽  
Dry Socket ◽  
Dental Hospital ◽  
Group A ◽  
Group B

Background: Dry socket is the most common post-operative complication following extraction of teeth. Various risk factors have been mentioned for this complication including gender, age, amount of trauma during extraction, difficulty of surgery, inappropriate irrigation, infection, smoking, and oral contraceptive use. Traditional treatment of dry socket require frequent visit of patient to the dental hospital and is very inconvenient to the patient.Objective: The aim of the study was to assess outcome of the treatment using two different techniques for the management of dry socket.Methods: Patients with dry socket were randomly distributed among two treatment groups (21 patients in each group). Group A received irrigation of socket with diluted hydrogen peroxide and zinc oxide eugenol dressing was given and patients were followed for following consecutive days by replacing dressing each day until the pain subsides. Group B received irrigation of socket with diluted hydrogen peroxide, sockets and surrounding gingival tissue were debrided to promote the re-establishment of blood clot. The gingival margins were meticulously sutured to protect the clot formation.Result 19 patients out of 21 in Group A, received two or more than two procedures to subside pain whereas in Group B, 19 out of 21 patients received just one procedure for successful treatment.Concusion: There is reduction in duration of treatment in debridement group of patients when compared with those treated traditionally.Journal of Nepalgunj Medical College Vol.12(1) 2014: 6-9

scholarly journals The Role of Superoxide and Hydrogen Peroxide in Group B Streptococcus-induced Pulmonary Hypertension in the Lamb 1622

1998 ◽  
Vol 43 ◽  
pp. 277-277
Author(s):  
D T Carpenter ◽  
H Larkin ◽  
J T O'Neill ◽  
M Abdelrahim ◽  
E Morris ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Pulmonary Hypertension ◽  
Group B Streptococcus ◽  
Group B

scholarly journals Composite resin shear bond strength on bleached dentin increased by 35% sodium ascorbate application

2017 ◽  
Vol 50 (4) ◽  
pp. 178
Author(s):  
Tunjung Nugraheni ◽  
N Nuryono ◽  
Siti Sunarintyas ◽  
Ema Mulyawati
Keyword(s):  
Hydrogen Peroxide ◽  
Bond Strength ◽  
Shear Bond Strength ◽  
Composite Resin ◽  
Artificial Saliva ◽  
Sodium Ascorbate ◽  
Group A ◽  
Group B ◽  
Group D ◽  
One Way Anova

Background: Restoration of the teeth immediately after bleaching with 35% hydrogen peroxide (H2O2) is contraindicated due to the remnant of free radicals that will stay inside enamel and dentin for 1-3 weeks and reduce the adhesion of composite resin. Sodium ascorbate is an antioxidant substance known to bind free radical residues, thereby shortening the delay in restoration. Purpose: The purpose of this study was to examine the resin bond strength of bleached dentin influenced by the application of 35% sodium ascorbate. Methods: Nine premolars were divided into their crown and root sections, with the crown subsequently being cut into four equal parts to obtain 36 samples. These were then divided into four groups, each containing nine samples. Group A (control): samples were bleached using35% hydrogen peroxide, immersed in an artificial saliva, stored in an incubator at 37°C for seven days and then filled with a composite resin. Group B:samples were also bleached by means of 35% H2O2 followed by one application of 0.025 ml 35% sodium ascorbate for 5 minutes and restored with composite resin. Group C: samples were bleached with 35% H2O2, followed by two applications of 0.025 ml 35% sodium ascorbate for 5 minutes, and restored with a composite resin. Group D: dentin was bleached with 35% H2O2 followed by three applications of 0.025 ml sodium ascorbate 35% for 5 minutes and restored with a composite resin. The shear bond strength of the composite resin was measured by a universal testing instrument (Zwick, USA). Data was analyzed by means of one-way Anova and LSD. Results: The highest mean shear bond strength of composite resin was in group C, while the lowest was in group B. The result of one-way Anova indicated a difference in the shear bond strength of composite resin in the four treatment groups (p < 0.05). An LSD test showed there to be a difference in shear bond strength of composite resin between group A and groups C and D or between group B and groups C and D. There was no difference in shear bond strength of composite resin between group A and group B or between group C and group D. Conclusion: Application frequency of 35% sodium ascorbate affect on shear bond strength of composite resin restoration in bleached dentin by 35% H2O2.

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