Hippocampal Neuronal Culture v1

2021 ◽  
Author(s):  
Daehun Park ◽  
Pietro De Camilli
Keyword(s):  
Neuronal Culture ◽  
Neuronal Cultures ◽  
New Born

This protocol describes the procedure for hippocampal neuronal cultures from new - born mouse pups.

scholarly journals Network science characteristics of brain-derived neuronal cultures deciphered from quantitative phase imaging data

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Chenzhong Yin ◽  
Xiongye Xiao ◽  
Valeriu Balaban ◽  
Mikhail E. Kandel ◽  
Young Jae Lee ◽  
...  
Keyword(s):  
Information Flow ◽  
Clustering Coefficient ◽  
Neuronal Culture ◽  
Node Degree ◽  
Neuronal Cultures ◽  
Phase Imaging ◽  
Imaging Data ◽  
Growth Data ◽  
Cluster Networks

Abstract Understanding the mechanisms by which neurons create or suppress connections to enable communication in brain-derived neuronal cultures can inform how learning, cognition and creative behavior emerge. While prior studies have shown that neuronal cultures possess self-organizing criticality properties, we further demonstrate that in vitro brain-derived neuronal cultures exhibit a self-optimization phenomenon. More precisely, we analyze the multiscale neural growth data obtained from label-free quantitative microscopic imaging experiments and reconstruct the in vitro neuronal culture networks (microscale) and neuronal culture cluster networks (mesoscale). We investigate the structure and evolution of neuronal culture networks and neuronal culture cluster networks by estimating the importance of each network node and their information flow. By analyzing the degree-, closeness-, and betweenness-centrality, the node-to-node degree distribution (informing on neuronal interconnection phenomena), the clustering coefficient/transitivity (assessing the “small-world” properties), and the multifractal spectrum, we demonstrate that murine neurons exhibit self-optimizing behavior over time with topological characteristics distinct from existing complex network models. The time-evolving interconnection among murine neurons optimizes the network information flow, network robustness, and self-organization degree. These findings have complex implications for modeling neuronal cultures and potentially on how to design biological inspired artificial intelligence.

scholarly journals Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons from Familial Parkinson’s Disease Patients Display α-Synuclein Pathology and Abnormal Mitochondrial Morphology

Cells ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2402
Author(s):  
Xiaojun Diao ◽  
Fei Wang ◽  
Andrea Becerra-Calixto ◽  
Claudio Soto ◽  
Abhisek Mukherjee
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Dopaminergic Neurons ◽  
Healthy Donor ◽  
Lewy Bodies ◽  
Neuronal Culture ◽  
Mitochondrial Morphology ◽  
Neuronal Cultures ◽  
Mitochondrial Abnormalities ◽  
Induced Pluripotent

Accumulation of α-synuclein (α-syn) into Lewy bodies (LBs) and mitochondrial abnormalities are the two cardinal pathobiological features of Parkinson’s disease (PD), which are associated with the loss of dopaminergic neurons. Although α-syn accumulates in many different cellular and mouse models, these models generally lack LB features. Here, we generated midbrain dopaminergic (mDA) neuronal cultures from induced pluripotent stem cells (iPSCs) derived from familial PD (fPD) patients and healthy controls. We show that mDA neuronal cultures from fPD patients with A53T mutation and α-syn gene (SNCA) triplication display pathological α-syn deposits, which spatially and morphologically resemble LBs. Importantly, we did not find any apparent accumulation of pathological α-syn in mDA neuronal culture derived from a healthy donor. Furthermore, we show that there are morphological abnormalities in the mitochondrial network in mDA neuronal cultures from fPD patients. Consequently, these cells were more susceptible to mitochondrial damage compared with healthy donor-derived mDA neuronal cultures. Our results indicate that the iPSC-derived mDA neuronal culture platform can be used to investigate the spatiotemporal appearance of LBs, as well as their composition, architecture, and relationship with mitochondrial abnormalities.

Ultrastructure and Staining of Developing Elastic Tissue

1971 ◽  
Vol 29 ◽  
pp. 244-245
Author(s):  
E. N. Albert
Keyword(s):  
Fine Structure ◽  
Phosphotungstic Acid ◽  
Staining Method ◽  
Rat Aorta ◽  
Uranyl Acetate ◽  
The Other ◽  
Elastic Fibers ◽  
Elastic Tissue ◽  
Lead Citrate ◽  
New Born

Silver tetraphenylporphine sulfonate (Ag-TPPS) was synthesized in this laboratory and used as an electron dense stain for elastic tissue (Fig 1). The procedures for the synthesis of tetraphenylporphine sulfonate and the staining method for mature elastic tissue have been described previously.The fine structure of developing elastic tissue was observed in fetal and new born rat aorta using tetraphenylporphine sulfonate, phosphotungstic acid, uranyl acetate and lead citrate. The newly forming elastica consisted of two morphologically distinct components. These were a central amorphous and a peripheral fibrous. The ratio of the central amorphous and the peripheral fibrillar portion changed in favor of the former with increasing age.It was also observed that the staining properties of the two components were entirely different. The peripheral fibrous component stained with uranyl acetate and/or lead citrate while the central amorphous portion demonstrated no affinity for these stains. On the other hand, the central amorphous portion of developing elastic fibers stained vigorously with silver tetraphenylporphine sulfonate, while the fibrillar part did not (compare figs 2, 3, 4). Based upon the above observations it is proposed that developing elastica consists of two components that are morphologically and chemically different.

Congenital heart malformation of new born in post mortem computed tomography

2013 ◽  
Vol 61 (S 01) ◽  
Author(s):  
B Vogel ◽  
H Gulbins ◽  
H Reichenspurner ◽  
A Heinemann ◽  
H Vogel
Keyword(s):  
Computed Tomography ◽  
Congenital Heart ◽  
Post Mortem ◽  
New Born ◽  
Heart Malformation

Mucous Cysts in the Thymus of the New Born Mouse

1978 ◽  
Vol 55 (2-3) ◽  
pp. 93-109 ◽  
Author(s):  
KIYOSHI KISHI
Keyword(s):  
New Born

THE LIVE WEIGHT GROWTH OF BULLS OF DIFFERENT BREEDS

2017 ◽  
Vol 53 ◽  
pp. 115-119
Author(s):  
A. V. Dymchuk ◽  
O. I. Lyubynskyy
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Dairy Cattle ◽  
Live Weight ◽  
Mathematical Statistics ◽  
High Quality ◽  
New Born ◽  
Black And White ◽  
Daily Average ◽  
Weight Growth

The studies of the dynamics results of body weight, absolute and daily average, relative increases averaging bulls of Ukrainian black-and-white and Ukrainian red-and-white dairy cattle. Researches are conducted in the conditions of POSP «Napadivske» Kalyinivka district of the Vinnytsya area after materials of primary pedigree account. In an economy use the whole-year of the same type feeding. In the structure of ration 40% make the concentrated sterns and also high-quality silage and soilage. Average daily increases of bulls for period of age to realization for a slaughter made over 1000 grammes. The dynamics of growth of living mass, absolute, average daily and relative increases of bulls, is analysed Ukrainian black-and-white and Ukrainian red-and-white dairy cattle. Living mass and weight gain researches of age to 12-monthly age with an interval in 3 months after the generally accepted methods. Materials of researches are worked out the methods of mathematical statistics with the use of programmatic package of Statistica 8.0. Living mass of bulls of the second group at birth made 36,3 kg, that anymore on 0,8 kg by comparison to new-born bulls of the first group. In all next periods living mass of animals of the second group was greater as compared to the animals of the same age of the first. In 3 months advantage made 1,7 kg, in 6 months – 4,6 kg (P>0,95), in 9 months – 8,8 kg (P>0,95), in 12 months – 12,5 kg (P>0,99). Difference of absolute increases of bulls of the second group above the bulls of the same age of the first of age to to 3-monthly age made 0,8 kg in behalf of animals of the second group. In a period 3-6 months a difference is after this index between the first and second groups made 2,9 kg in behalf of the last. In periods 6-9 and reliable advantage of animals of the second group is 9-12 months set above the bulls of the same age of the first on 4,1 kg (P>0,95) and 3,7 kg (P>0,95) accordingly. In a period of age to 18-monthly age absolute increases of bulls of the second group were greater by comparison to bulls first on 12,5 kg (R>0,99). Average daily increases of bulls of the second group of age to 3-monthly age made 763,0 grammes, that anymore on 8,9 grammes by comparison to bulls of the first group. In periods 3-6 months the average daily increases of animals of the second group prevailed the bulls of the same age of the first on 32,6 grammes. In a period 6-9 months the average daily increases of animals of the second group were made by 1045,9 grammes, that for certain anymore on 45,9 grammes (P>0,95) by comparison to the bulls of the same age of the first. Maximal increases of bulls were in a period 9-12 months and made 1210,4 grammes in the animals of the second group and 1168,9 grammes – for the bulls of the same age of the first, that for certain anymore on 41,5 grammes (P>0,95). In a period of age to 12-monthly age the average daily increases of bulls of the second group were made by 970,6 grammes, that was for certain anymore on 31,8 grammes (R>0,99) by comparison to the bulls of the same age of the first group. Relative increases of bulls of both groups were high. From birth to 3-monthly age greater increases had bulls of the first group – 97,8%, and in periods 3-6, 6-9 and 9-12 months greater were increases of bulls of the same age of the second group – 56,4; 40,1 and 32,4% accordingly. For all probed periods of reliable advantage it is not discovered between the compared groups. It is set researches, that at the identical terms of feeding and maintenance more rapid bulls grow Ukrainian red-and-white dairy cattle. In all age-old periods they prevailed the bulls of the same age of the Ukrainian black-and-white dairy cattle, in a that number, beginning from 9-monthly age, advantage was reliable (P>0,95–0,99). Average daily increases of bulls of two breeds were the least in a period of age to to 3-monthly age – 754,1-763,0 grammes, and most in a period 9-12 months – 1168,9–1210,4 grammes.

Creative Methodologies of Da’wah Through Different Phases of a Human’s Life (Metodologi Dakwah Secara Kreatif Mengikut Fasa Kehidupan Manusia)

2018 ◽  
Vol 15 (1) ◽  
pp. 271-293
Author(s):  
Maulana Akbar Shah @ U Tun Aung
Keyword(s):  
Married Couples ◽  
Special Group ◽  
Target Groups ◽  
New Born ◽  
Special Groups ◽  
Pregnant Mothers ◽  
Do So

Abstract In addition to compliance with the five pillars of Islam, Da’wah Islamiyyah is an indispensable act for Muslims who view their life missions as to propagate Islam. The act of da’wah is a mission only undertaken by those who are selected to do so, as Allah SWT said: “You are the best of peoples, taken out for mankind, you enjoin what is right, forbid what is wrong…,” (Al-Qur’an, Al- `Imran, 3:110). The phrase “you are taken out for mankind” is a very clear phrase which refers to a special group. Many du’at propagate Islam through regular procedures. In this contemporary day and age, it would be more successful if creative da’wah methodologies are used to special groups where special talent is required. Examples of these special target groups are individuals such as [1] pregnant mothers, [2] mothers of new born babies, [3] parents of toddlers, [4] parents of children [5] youth, teenagers and adolescents, [6] married couples, [7] elderly or aged individuals and [8] non-Muslims.  They are good targets to promote Islam. Thus, du’at must have special talents and knowledge to do so. In this regard, du’at must be equipped with exceptional knowledge of the target groups to propagate Islam effectively. The present paper attempts to highlight the distinctive natures of the above mentioned categories of individuals and explores how du’at should approach them when he or she encounters them for the purpose of promoting Islam.        Keywords: Call to Islam, Da’wah Islamiyyah, creative da’wah, creative da’ie, enjoin good, forbid evil.   Abstrak Selain mematuhi 5 rukun Islam, dakwah Islamiyyah tidak dapat diasingkan daripada seseorang Muslim yang bermatlamat untuk menyebarkan ajaran Islam. Berdakwah adalah suatu tugas yang hanya dijalankan oleh mereka yang terpilih. Seperti yang diwahyukan oleh Allah SWT “Kamu adalah sebaik-baik umat yang dilahirkan bagi umat manusia, kerana kamu menyuruh berbuat segala perkara yang baik dan melarang daripada segala perkara yang buruk dan keji...” (Al-Quran, Al-Imran, 3:110). Frasa “umat yang dilahirkan bagi umat manusia”  jelas dinyatakan di mana ia merujuk kepada satu golongan yang istimewa. Kebanyakan pendakwah menyebarkan Islam melalui metodologi yang sudah menjadi kebiasaan. Bagaimanapun, pada zaman ini, pendakwahan akan lebih berjaya jika kaedah yang kreatif digunakan kepada beberapa golongan yang tertentu. Antara contoh golongan yang dimaksudkan adalah [1] wanita yang mengandung, [2] ibu kepada bayi yang baru lahir, [3] ibu bapa kepada anak-anak kecil, [4] ibu bapa kepada kanak-kanak, [5] golongan belia dan remaja, [6] pasangan suami isteri, [7] warga emas, dan [8] golongan bukan Islam. Mereka merupakan sasaran yang sempurna untuk mempelajari Islam. Oleh hal demikian, para pendakwah mesti mempunyai bakat yang istimewa dan ilmu yang mencukupi untuk berdakwah. Para pendakwah juga harus memahami kumpulan sasaran dengan menyeluruh supaya penyebaran ajaran Islam akan lebih berkesan. Tujuan penulisan ini adalah untuk menggariskan sifat unik golongan yang dinyatakan di atas dan menerokai bagaimana para pendakwah harus mendekati mereka dengan niat untuk menyebarkan Islam.   Kata Kunci: Panggilan kepada Islam, dakwah Islamiyyah, dakwah kreatif, da’ie kreatif, menyuruh kepada kebaikan, menghalang daripada kejahatan.  

Chemical Effects and Predictive Factors in Premature Birth

2018 ◽  
Vol 69 (7) ◽  
pp. 1796-1801
Author(s):  
Adrian Neacsu ◽  
Alina Calin ◽  
Anca Daniela Braila ◽  
Dan Navolan ◽  
Mihai Dimitriu ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Premature Birth ◽  
Tissue Concentration ◽  
Premature Labor ◽  
New Born ◽  
Uterine Contractions ◽  
Chemical Effects ◽  
Equal Importance ◽  
Necrosis Factor

Premature birth is considered to be the consequence of independent alterations in the cervix and in the uterus. During labor, for full-term birth, as well as for premature birth, the cervix changes, from firm, long and closed, to soft and pliable, through a biochemical process characterized by the reshaping of the extracellular matrix and a growth of the tissue concentration of inflammatory mediators; the uterus proves an increase in contractility and sensitivity to endogenic hormones, such as oxytocin. Premature labor is associated with the premature activation of the release of cytokines in the decidua (mucosa lining uterus walls) and cervix. Interleukins IL-1 beta, IL-6, IL-8 and the alpha tumoral necrosis factor increase the production and activation of matrix metalloproteinases (MMP-1, MMP-3 and MMP-9) and of cathepsin S, which digests the collagen from the extracellular matrix of the cervix, causing the wiping and softening of the cervix. These cytokines are released by leukocytes in the myometer, leading to the production of prostaglandins and oxytocin, which stimulate uterine contractions. Therefore, the cervical shortening represented by ultrasound is believed to represent premature cervical softening. The obstetrical approach of aspects related to premature birth are based, considerably, on the prognosis expected by the obstetrician regarding the survival of the premature new-born baby, as well as the therapeutic variants to be followed. And not only survival is important, of equal importance is also the quality of life of underweight, immature new-born babies, who are considerably affected both physically, and intellectually.

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