scholarly journals Protein expression in OnePot PURE cell-free system v1 (protocols.io.8avhse6)

protocols.io ◽  
2019 ◽  
Author(s):  
Konstantinos Ragios
Keyword(s):  
Protein Expression ◽  
Free System ◽  
Cell Free System ◽  
Pure Cell

scholarly journals A simple, robust, and low-cost method to produce the PURE cell - free system

2018 ◽  
Author(s):  
Barbora Lavickova ◽  
Sebastian J. Maerkl
Keyword(s):  
Protein Synthesis ◽  
Low Cost ◽  
Translation System ◽  
Free System ◽  
Cell Free System ◽  
Pure System ◽  
Pure Cell ◽  
Synthesis Yield

AbstractWe demonstrate a simple, robust, and low-cost method for producing the PURE cell-free transcription-translation system. Our OnePot PURE system achieved a protein synthesis yield of 156 µg/mL at a cost of 0.09 USD/µL, leading to a 14-fold improvement in cost normalized protein synthesis yield over existing PURE systems. The OnePot method makes the PURE system easy to generate and allows it to be readily optimized and modified.

Preliminary study on preparation of E. coli cell-free system for protein expression

2008 ◽  
Vol 2 (2) ◽  
pp. 224-229 ◽  
Author(s):  
Haiqin Chen ◽  
Zhinan Xu ◽  
Cheng Wang ◽  
Yuhua Liao ◽  
Peilin Cen
Keyword(s):  
Protein Expression ◽  
Coli Cell ◽  
Free System ◽  
Cell Free System ◽  
E Coli ◽  
Preliminary Study

A second rhodopsin-like protein in Cyanophora paradoxa: Gene sequence and protein expression in a cell-free system

2013 ◽  
Vol 125 ◽  
pp. 188-193 ◽  
Author(s):  
Anna Maria Frassanito ◽  
Laura Barsanti ◽  
Vincenzo Passarelli ◽  
Valtere Evangelista ◽  
Paolo Gualtieri
Keyword(s):  
Protein Expression ◽  
Gene Sequence ◽  
Cyanophora Paradoxa ◽  
Free System ◽  
Cell Free System ◽  
A Cell

Functional protein expression from a DNA based wheat germ cell-free system

2007 ◽  
Vol 8 (4) ◽  
pp. 199-208 ◽  
Author(s):  
Kate Qin Zhao ◽  
Robin Hurst ◽  
Michael R. Slater ◽  
Robert F. Bulleit
Keyword(s):  
Protein Expression ◽  
Germ Cell ◽  
Wheat Germ ◽  
Functional Protein ◽  
Free System ◽  
Cell Free System
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