the RNA extract protocol v1 (protocols.io.2uagese)

The effect of exogenous RNA extract on chick embryo fibroblasts in vitro

Development ◽

10.1242/dev.23.2.509 ◽

1970 ◽

Vol 23 (2) ◽

pp. 509-517

Author(s):

A. Sann ◽

D. Sharp ◽

J. McKenzie

Keyword(s):

Cell Differentiation ◽

Chick Embryo ◽

Morphological Changes ◽

Bacterial Cells ◽

Baby Hamster Kidney ◽

Exogenous Rna ◽

Embryo Fibroblasts ◽

Stimulation Of ◽

Rna Extract

It is extremely difficult, if not impossible, to reconcile the conflicting claims of those who have treated different cells and tissues with exogenous RNA. Some authors (e.g. Niu, Cordova & Niu, 1961; Niu, Cordova & Radbill, 1962) maintain that RNA extracts alter the course of cell differentiation to conform in morphological terms to the source of the RNA; in the same vein, Amos, Askonas & Soeiro (1964) have shown that, under certain conditions, RNA from mouse and bacterial cells can stimulate chick embryo fibroblasts to synthesize protein related antigenically to the origin of the RNA. Shepley, Ambrose & Kirby (1965), however, obtained stimulation of growth with permanent morphological changes in baby hamster kidney fibroblasts by the addition of RNA from a variety of sources.

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Induction in vitro of Antibodies to Phage T2: Antigens in the RNA Extract Employed

Science ◽

10.1126/science.149.3688.1106 ◽

1965 ◽

Vol 149 (3688) ◽

pp. 1106-1107 ◽

Cited By ~ 106

Author(s):

H. P. Friedman ◽

A. B. Stavitsky ◽

J. M. Solomon

Keyword(s):

Rna Extract

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Facilitation of Discrimination Learning by Injection of an RNA Extract Prepared from Donor Subjects

Psychological Reports ◽

10.2466/pr0.1967.21.2.601 ◽

1967 ◽

Vol 21 (2) ◽

pp. 601-605 ◽

Cited By ~ 2

Author(s):

Wayne Viney ◽

John C. Branch ◽

William E. Gill

Keyword(s):

Discrimination Learning ◽

Discrimination Task ◽

Reliable Difference ◽

Brain Extracts ◽

Position Discrimination ◽

Rna Extract

Naive rats given intraperitoneal injections of an extract prepared from the brains of either trained or untrained donor rats learned a position discrimination task in simple parallel alley runways significantly faster than original (uninjected) donor rats. Specific interanimal transfer, however, was not demonstrated as there was no statistically reliable difference between recipients of “trained brain” vs “untrained brain” extracts.

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Growth and nitrogen metabolism of sea bass fed graded levels of nucleic acid nitrogen from yeast or RNA extract as partial substitute for protein nitrogen from fish meal

Italian Journal of Animal Science ◽

10.4081/ijas.2005.2s.600 ◽

2005 ◽

Vol 4 (sup2) ◽

pp. 600-602 ◽

Cited By ~ 1

Author(s):

F. Tulli ◽

C. Franchin ◽

E. Tibaldi ◽

S. Kaushik

Keyword(s):

Nucleic Acid ◽

Nitrogen Metabolism ◽

Fish Meal ◽

Sea Bass ◽

Protein Nitrogen ◽

Rna Extract

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An Attempt to Transfer a Position Discrimination Habit via RNA Extracts

Psychological Reports ◽

10.2466/pr0.1966.19.3.923 ◽

1966 ◽

Vol 19 (3) ◽

pp. 923-926 ◽

Cited By ~ 5

Author(s):

John C. Branch ◽

Wayne Viney

Keyword(s):

Transfer Of Learning ◽

Learning Effects ◽

Discrimination Habit ◽

Position Discrimination ◽

Rna Extract

RNA extracted from trained and untrained (control) rats did not demonstrate statistically significant transfer of learning effects when injected intraperitoneally into naive recipient rats. Since the direction of the results consistently favored the “trained RNA extract” recipient, it is suggested that future investigation of biochemical transfer utilize more rigorous training for donor animals and larger RNA dosage levels for injections of recipient animals.

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