scholarly journals Development and Validation of Stability Indicating RP-HPLC Method for Simultaneous Estimation of Ibuprofen and Carisoprodol in Pharmaceutical Formulation

2019 ◽  
Vol 3 (1) ◽  
pp. 072-075
Author(s):  
A Lakshmana Rao ◽  
P Vijetha
Keyword(s):  
Hplc Method ◽  
Pharmaceutical Formulation ◽  
Simultaneous Estimation ◽  
Stability Indicating ◽  
Rp Hplc ◽  
Development And Validation

scholarly journals Development and validation of simultaneous estimation of drugs in combination from pharmaceutical formulation by RP-HPLC method

2020 ◽  
Vol 12 (3) ◽  
pp. 155-165
Author(s):  
Pooja Eknathji Waware ◽  
Anuja Dattatriayji Jolhe ◽  
Swati Rajendra Lilhare ◽  
Prasad Prakash Jumade ◽  
Dharmendra Ramgopalji Mundhada
Keyword(s):  
Hplc Method ◽  
Pharmaceutical Formulation ◽  
Simultaneous Estimation ◽  
Rp Hplc ◽  
Development And Validation

DEVELOPMENT AND VALIDATION OF STABILITY INDICATING RP-HPLC METHOD FOR THE SIMULTANEOUS DETERMINATION OF BERBERINE AND CURCUMIN IN AN AYURVEDIC FORMULATION

INDIAN DRUGS ◽  
2016 ◽  
Vol 53 (08) ◽  
pp. 48-52
Author(s):  
K. P Parekh ◽  
◽  
A. P. Jadhav
Keyword(s):  
Mobile Phase ◽  
Herbal Medicines ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Quality And Safety ◽  
Stability Indicating ◽  
Rp Hplc ◽  
Water Ph ◽  
Development And Validation

A simple, accurate, precise, robust stability indicating RP-HPLC method was developed and validated for simultaneous estimation of berberine and curcumin in an ayurvedic formulation. The two markers were resolved using a C-18 column using as the mobile phase methanol: water (pH 3 adjusted using acetic acid) in the ratio 75:25 V/V at a flow rate of 1mL/min. Retention times of berberine and curcumin were 2.58 ± 0.2 min and 8.5 ± 0.2 min, respectively at 358 nm. Linear response was observed in the concentration range of 2 – 8 ppm for berberine and 5 – 40 ppm for curcumin, with correlation coefficient (r2) of 0.994 and 0.998 for berberine and curcumin, respectively. The developed method was applied for quantitation of markers in marketed and in-house formulations of Gruhadhoomadi Churna. This method can also be used to evaluate formulations containing berberine and curcumin as markers, thus conforming to the need of ensuring quality and safety of herbal medicines.

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