scholarly journals Meticillin-resistant Staphylococcus aureus isolated from Iranian hospitals: virulence factors and antibiotic resistance properties

2014 ◽  
Vol 14 (4) ◽  
pp. 219 ◽  
Author(s):  
Hassan Momtaz ◽  
Laleh Hafezi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Virulence Factors ◽  
Antibiotic Resistance Properties

scholarly journals Molecular Typing and Phenotypic and Genotypic Evaluation of Antibiotic Resistance and Virulence Factors of the Methicillin-Resistant Staphylococcus Aureus Bacteria Isolated From Vegetable and Salad Samples

2020 ◽  
Author(s):  
Bahareh Tavakoli-Far ◽  
Bita Mousavi ◽  
Zohreh Mashak ◽  
Mohammad Adel Rezaei ◽  
Fatemeh Doregiraee ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Virulence Factors ◽  
Resistance Genes ◽  
Molecular Typing ◽  
Antibiotic Resistance Genes ◽  
Disk Diffusion ◽  
Bacteria Resistance ◽  
Antibiotic Agents ◽  
Antibiotic Resistance Properties

Abstract BackgroundMethicillin-resistant Staphylococcus aureus is an important cause of foodborne diseases. The present research evaluated the antibiotic resistance properties, distribution of virulence factors, and molecular typing of MRSA bacteria isolated from vegetable and salad samples. MethodsThree-hundred and fifty vegetable and salad samples were examined for the presence of S. aureus using the culture. MRSA bacteria were identified using cefoxitin and oxacillin disk diffusion. The phenotypic pattern of antibiotic resistance was assessed by disk diffusion. ResultsThe PCR evaluated the distribution of antibiotic resistance and virulence genes. Forty-five out of 350 (12.85%) vegetable and salad samples were positive for S. aureus. Twenty-six isolates out of 45 (57.77%) S. aureus bacteria were determined as MRSA. MRSA bacteria harbored the uppermost prevalence of resistance against cefoxitin (100%), ceftaroline (100%), penicillin (100%), tetracycline (88.46%), gentamicin (80.76%), trimethoprim-sulfamethoxazole (69.23%), and erythromycin (69.23%). The prevalence of MRSA bacteria resistance recovered from vegetable and salad samples against more than seven antibiotic agents was 12.50% and 27.77%, respectively. BlaCTX-M (100%), blaZ (100%), aacA-D (61.53%), tetK (57.69%), dfrA1 (46.15%), and vanA (42.30%) were the most commonly detected antibiotic resistance genes. PVL (57.69%), coa (53.84%), and hla (38.46%) were the most commonly detected virulence factors amongst the MRSA bacteria. ConclusionMRSA isolates had a similarity lower than 80%, categorized in the same group. The presence of one or more virulence factors and antibiotic resistance genes amongst the resistant-MRSA bacteria signifies an important threat rendering the consumption of contaminated vegetables and salads.

A review of virulence factors, pathogenesis, and antibiotic resistance in Staphylococcus aureus

2016 ◽  
Vol 27 (2) ◽  
pp. 50-56 ◽  
Author(s):  
Nada Fahad Al-Mebairik ◽  
Talat A. El-Kersh ◽  
Yazeed A. Al-Sheikh ◽  
Mohammed Ali M. Marie
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Virulence Factors

scholarly journals Going through phages: A Computational approach to Revealing the role of prophage in Staphylococcus aureus

2021 ◽  
Author(s):  
Tyrome Steven Sweet ◽  
Suzanne Sindi ◽  
Mark Sistrom
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Genome Evolution ◽  
Virulence Factors ◽  
Rapid Growth ◽  
Computational Approach ◽  
Computational Pipeline ◽  
Genes Encoding

Prophages have important roles in virulence, antibiotic resistance and genome evolution in Staphylococcus aureus. Rapid growth in the number of sequenced S. aureus genomes allows for an investigation of prophage sequences in S. aureus at an unprecedented scale. We developed a computational pipeline to detect and analyze prophage sequences in nearly 10,011 S. aureus genomes, discovering thousands of putative prophage sequences with genes encoding virulence factors and antibiotic resistance.

scholarly journals 2602. Genetic Basis of Staphylococcus aureus Virulence

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S904-S904
Author(s):  
Edward W Adams ◽  
Doyle V Ward ◽  
Bruce A Barton ◽  
Richard T Ellison ◽  
Oladapo Olaitan
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Virulence Factors ◽  
Resistance Genes ◽  
Gene Frequency ◽  
Academic Medical Center ◽  
Clinical Manifestations ◽  
Antibiotic Resistance Genes ◽  
Convenience Sample ◽  
Tract Infections

Abstract Background Although multiple different virulence factors have been identified for Staphylococcus aureus, there is limited information on genetic variation present between different strains of S. aureus in the clinical setting. To better define whether differing virulence factors could contribute to differing clinical manifestations of S. aureus infections we undertook a comparison of the frequency of virulence and antibiotic resistance genes present in S. aureus isolates from different clinical sites. Methods Whole-genome sequencing was performed on a convenience sample of S. aureus isolates from clinical or surveillance cultures obtained at an academic medical center over a 27-month period. Genomic assemblies were generated and annotated to define protein-coding regions. The prevalence of 28 genes previously defined as being associated with S. aureus virulence or antimicrobial resistance, including MSCRAMM genes, was then analyzed in relation to nine specific culture sources including only a single isolate from each culture source per patient using a likelihood ratio χ 2 analysis. Results There were 1286 S. aureus isolates with draft assemblies and annotations, and there was a statistically significant (P < 0.01) difference in gene frequencies between culture sources for 18 genes that included 13 of 19 virulence factors, 4 of 7 antibiotic resistance genes and 1 of 2 MSCRAMM genes. The most notable variation was seen for the presence of the sec, sep, entB, lukS, lufK, fosB, mecA, and ermA genes (all with P < 0.0001). There were also significant variations in overall gene frequency patterns between isolates from wound, blood, and respiratory isolates (P < 0.0001), as well as significant differences in the frequency of cna and hlY genes between surveillance and clinical isolates (P < 0.0001). Conclusion This study demonstrates a difference in the prevalence of virulence and antibiotic resistance genes in S. aureus isolates based on the culture source. As the culture location can be considered a surrogate for different types of infections (such as bacteremia, pneumonia, urinary tract infections) these differences in gene frequency may contribute to variation in the clinical manifestations of infections by differing S. aureus strains. Disclosures All authors: No reported disclosures.

A new anti-infective strategy to reduce the spreading of antibiotic resistance by the action on adhesion-mediated virulence factors in Staphylococcus aureus

2013 ◽  
Vol 63 ◽  
pp. 44-53 ◽  
Author(s):  
Rosanna Papa ◽  
Marco Artini ◽  
Andrea Cellini ◽  
Marco Tilotta ◽  
Eugenio Galano ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Virulence Factors
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