scholarly journals Development of fertility restorers of winter oilseed rape with low glucosinolate content for the CMS Ogu-INRA system

2009 ◽  
Vol 45 (No. 3) ◽  
pp. 123-127 ◽  
Author(s):  
R. Koprna ◽  
V. Kučera ◽  
I. Macháčková ◽  
J. Horáček ◽  
J. Ehrenbergerová
Keyword(s):  
Oilseed Rape ◽  
Scar Marker ◽  
Restorer Gene ◽  
Glucosinolate Content ◽  
Restorer Line ◽  
Winter Oilseed Rape ◽  
Fertility Restorer ◽  
Fertility Restorer Gene ◽  
Fertility Restorers ◽  
Selection Of

We have bred low glucosinolate (GSL) winter oilseed rape lines carrying the fertility restorer for the CMS Ogu-INRA system. The original restorer line BO20 contained 31μmol/g GSL in seeds, but by crossing this line with various low GSL CMS lines, followed by repeated selection of fertile segregants, we were able to obtain fertile lines with a mean GSL content in seeds of 11.8 μmol/g. This result confirmed that the gene(s) controlling the GSL content are not closely linked to the fertility restorer gene. The results confirm, that the SCAR marker SG34 is closely associated with the fertility restoring allele, and facilitates so the selection of fertile segregants; however, the marker is unable to distinguish between the homozygous RfRf and the heterozygous Rfrf genotypes.

scholarly journals Non-destructive in vitro selection of microspore-derived embryos with the fertility restorer gene for CMS Ogu-INRA in winter oilseed rape (Brassica napus L.)

2015 ◽  
Vol 18 (1) ◽  
pp. 58-60 ◽  
Author(s):  
Lenka Havlíčková ◽  
Miroslav Klíma ◽  
Marie Přibylová ◽  
Alois Antonín Hilgert-Delgado ◽  
Vratislav Kučera ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
In Vitro Selection ◽  
Winter Oilseed Rape ◽  
Fertility Restorer ◽  
Brassica Napus L ◽  
Fertility Restorer Gene ◽  
Non Destructive ◽  
Selection Of

Marker-assisted identification of potential fertility restorers for the development of three-line hybrids in rice (Oryza sativa L.)

2020 ◽  
Vol 57 (3) ◽  
pp. 181-189
Author(s):  
Asma Majid ◽  
GA Parray ◽  
NR Sofi ◽  
Gazala H Khan ◽  
Showkat A Waza ◽  
...  
Keyword(s):  
Oryza Sativa L ◽  
Chromosome 1 ◽  
Limiting Factor ◽  
Kashmir Valley ◽  
Fertility Restorer ◽  
Yield And Quality ◽  
Restorer Genes ◽  
Increase Productivity ◽  
Fertility Restorers ◽  
Selection Of

Rice being a staple food crop of Kashmir valley, the focus is on enhancement of yield in order to meet the needs of ever-growing population.Identification of new parental lines is crucial for developing ecology-specific hybrids with ideal agronomic performance. Exploitation of heterosis in the form of hybrid rice technology can be one of the approaches to increase productivity in this crop, especially exploiting diversity among japonica lines can serve as an excellent route.A number of CMS lines suitable formountainous areas of Kashmir have been developed, however, the availability of promising restorer lines remains to be the major limitation for utilization of these lines.Identification of potential restorers acts as the main limiting factor for hybrid development in the Kashmir valley. Marker based screening for Rf3 and Rf4 fertility restorer genes can be helpful in rapid selection of restorer lines while dealing with the large quantity of genetic materials. In the present study, 100 rice germplasm were screened with the help of SSR markers, RM3148 and RM6100linked to Rf3 and Rf4 genes on chromosome 1 and 10, respectively. In total, 19 lines revealed the presence of both Rf3 and Rf4 genes. These lines amplified fertility restorer specific alleles for both the genes and may serve as potential restorers for obtaining heterotic rice hybrids. Further the germplasm lines were also evaluated for yield and quality traits.The present results would help in selection of suitable restorers along with preferred grain shape/size.

scholarly journals A single nucleotide substitution in the coding region of Ogura male sterile gene, orf138, determines effectiveness of a fertility restorer gene, Rfo, in radish

2021 ◽  
Author(s):  
Hiroshi Yamagishi ◽  
Megumi Jikuya ◽  
Kanako Okushiro ◽  
Ayako Hashimoto ◽  
Asumi Fukunaga ◽  
...  
Keyword(s):  
Male Sterility ◽  
Nucleotide Substitution ◽  
Fertility Restoration ◽  
Type A ◽  
Restorer Gene ◽  
Single Nucleotide Substitution ◽  
Coding Region ◽  
Fertility Restorer ◽  
Single Nucleotide ◽  
Fertility Restorer Gene

AbstractCytoplasmic male sterility (CMS) observed in many plants leads defect in the production of functional pollen, while the expression of CMS is suppressed by a fertility restorer gene in the nuclear genome. Ogura CMS of radish is induced by a mitochondrial orf138, and a fertility restorer gene, Rfo, encodes a P-type PPR protein, ORF687, acting at the translational level. But, the exact function of ORF687 is still unclear. We found a Japanese variety showing male sterility even in the presence of Rfo. We examined the pollen fertility, Rfo expression, and orf138 mRNA in progenies of this variety. The progeny with Type H orf138 and Rfo showed male sterility when their orf138 mRNA was unprocessed within the coding region. By contrast, all progeny with Type A orf138 were fertile though orf138 mRNA remained unprocessed in the coding region, demonstrating that ORF687 functions on Type A but not on Type H. In silico analysis suggested a specific binding site of ORF687 in the coding region, not the 5′ untranslated region estimated previously, of Type A. A single nucleotide substitution in the putative binding site diminishes affinity of ORF687 in Type H and is most likely the cause of the ineffectiveness of ORF687. Furthermore, fertility restoration by RNA processing at a novel site in some progeny plants indicated a new and the third fertility restorer gene, Rfs, for orf138. This study clarified that direct ORF687 binding to the coding region of orf138 is essential for fertility restoration by Rfo.

scholarly journals Marker assisted selection of new high oleic and low linolenic winter oilseed rape (Brassica napus L.) inbred lines revealing good agricultural value

PLoS ONE ◽  
2020 ◽  
Vol 15 (6) ◽  
pp. e0233959 ◽  
Author(s):  
Stanisław Spasibionek ◽  
Katarzyna Mikołajczyk ◽  
Hanna Ćwiek–Kupczyńska ◽  
Teresa Piętka ◽  
Krystyna Krótka ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Marker Assisted Selection ◽  
Inbred Lines ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
High Oleic ◽  
Low Linolenic ◽  
Selection Of

Characterization of a radish introgression carrying the Ogura fertility restorer gene Rfo in rapeseed, using the Arabidopsis genome sequence and radish genetic mapping

2003 ◽  
Vol 107 (8) ◽  
pp. 1442-1451 ◽  
Author(s):  
Sandra Giancola ◽  
Sylvie Marhadour ◽  
Sophie Desloire ◽  
Vanessa Clouet ◽  
Hélène Falentin-Guyomarc'h ◽  
...  
Keyword(s):  
Genetic Mapping ◽  
Genome Sequence ◽  
Restorer Gene ◽  
Arabidopsis Genome ◽  
Fertility Restorer ◽  
Fertility Restorer Gene
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