scholarly journals Production of biogenic amines by Enterococci

2010 ◽  
Vol 27 (Special Issue 2) ◽  
pp. 50-55 ◽  
Author(s):  
K. Kučerová ◽  
H. Svobodová ◽  
Š. Tůma ◽  
I. Ondráčková ◽  
M. Plocková
Keyword(s):  
Growth Medium ◽  
Histidine Decarboxylase ◽  
Cow Milk ◽  
Decarboxylase Activity ◽  
Differential Growth ◽  
Specific Pcr ◽  
Enterococcus Spp ◽  
Pcr Method ◽  
Species Specific ◽  
Histidine Decarboxylase Activity

Enterococci were presented in all tested samples of raw cow milk (six samples) at the level 10<sup>3</sup>&ndash;10<sup>5</sup> CFU/ml, fresh cheeses (five samples) at the level 10<sup>2</sup>&ndash;10<sup>6</sup> CFU/g and semi-hard cheeses (five samples) at the level 10<sup>3</sup>&ndash;10<sup>5</sup> CFU/g. All 33 isolated Enterococcus strains were screened for decarboxylase activity by usage differential growth medium and 20 of them possessed tyrosine decarboxylase activity. A collection of eight strains with the strongest decarboxylase activity were identified by species specific PCR as E. faecium (Z3, Z4, Br4 and 6/4D strains) and E. faecalis (Ž4, 3/3C and 4/1A strains). Enterococcus spp. Z1 strain was not identified at the species level by used methods, but the genus was confirmed by PCR method. Their tyrosin decarboxylase activity was confirmed by TLC and detection of tdc gene. Z1, Z3 and Z4 strains showed also histidine decarboxylase activity on the differential growth medium with histidine, but this activity was evaluated by TLC as a false positive reaction of medium.

Mast cells and histamine concentration in muscle and liver of dystrophic mice

1964 ◽  
Vol 206 (2) ◽  
pp. 338-340 ◽  
Author(s):  
Pierre Bois
Keyword(s):  
Mast Cells ◽  
Histidine Decarboxylase ◽  
Binding Capacity ◽  
The Other ◽  
Decarboxylase Activity ◽  
Histamine Concentration ◽  
Other Hand ◽  
Histamine Binding ◽  
Dystrophic Mice ◽  
Histidine Decarboxylase Activity

The distribution of mast cells in muscle and liver of dystrophic mice was studied; histamine and histidine decarboxylase activity was also measured in the same tissues. Mast cells were significantly more numerous in dystrophic muscles. On the other hand, very few cells could be counted in the liver of either control or dystrophic animals. Histamine concentration was higher in muscle and liver of dystrophic mice; no visible increase in histidine decarboxylase activity could be measured by the methods used. It is concluded that histamine-binding capacity is increased in some tissues of dystrophic mice.

Regulation of rat stomach histidine decarboxylase activity by the serum gastrin concentration

1973 ◽  
Vol 3 (3) ◽  
pp. 178-179 ◽  
Author(s):  
R. Håkanson ◽  
G. Liedberg ◽  
J. Oscarson ◽  
J. F. Rehfeld ◽  
F. Stadil
Keyword(s):  
Histidine Decarboxylase ◽  
Serum Gastrin ◽  
Decarboxylase Activity ◽  
Rat Stomach ◽  
Serum Gastrin Concentration ◽  
Histidine Decarboxylase Activity

Elevation of histidine decarboxylase activity in skeletal muscles and stomach in mice by stress and exercise

2000 ◽  
Vol 279 (6) ◽  
pp. R2042-R2047 ◽  
Author(s):  
Kentaro Ayada ◽  
Makoto Watanabe ◽  
Yasuo Endo
Keyword(s):  
Muscle Activity ◽  
Skeletal Muscles ◽  
Histidine Decarboxylase ◽  
Muscle Tension ◽  
The Other ◽  
Decarboxylase Activity ◽  
Gastric Hemorrhage ◽  
Different Types ◽  
Cold Restraint ◽  
Histidine Decarboxylase Activity

The effects of different types of stress (water bathing, cold, restraint, and prolonged walking) on histidine decarboxylase (HDC) activity in masseter, quadriceps femoris, and pectoralis superficial muscles, and in the stomach were examined in mice. All of these stresses elevated gastric HDC activity. Although water bathing, in which muscle activity was slight, was sufficiently stressful to produce gastric hemorrhage and to increase gastric HDC activity, it produced no detectable elevation of HDC activity in any of the muscles examined. The other stresses all elevated HDC activity in all three muscles. We devised two methods of restraint, one accompanied by mastication and the other not. The former elevated HDC activity in the masseter muscle, but the latter did not. These results suggest that 1) HDC activity in the stomach is an index of responses to stress, 2) the elevation of HDC activity in skeletal muscles during stress is induced partly or wholly by muscle activity and/or muscle tension, and 3) stress itself does not always induce an elevation of HDC activity in skeletal muscles.

scholarly journals Design of a species-specific PCR method for the detection of the heat-resistant fungi Talaromyces macrosporus and Talaromyces trachyspermus

2017 ◽  
Vol 66 (1) ◽  
pp. 86-92 ◽  
Author(s):  
S. Yamashita ◽  
H. Nakagawa ◽  
T. Sakaguchi ◽  
T-H. Arima ◽  
Y. Kikoku
Keyword(s):  
Heat Resistant ◽  
Specific Pcr ◽  
Pcr Method ◽  
Species Specific

Histidine Decarboxylase Activity of Enterobacter cloacae S15/19 during the Production of Ripened Sausages and Its Influence on the Formation of Cadaverine

1997 ◽  
Vol 60 (4) ◽  
pp. 430-432 ◽  
Author(s):  
ARTUR X. ROIG-SAGUÉS ◽  
MANUELA HERNÁNDEZ-HERRERO ◽  
JOSE J. RODRÍGUEZ-JEREZ ◽  
EMILIO I. LÓPEZ-SABATER ◽  
MARIA T. MORA-VENTURA
Keyword(s):  
Production Process ◽  
Fecal Coliform ◽  
Histidine Decarboxylase ◽  
Raw Materials ◽  
Enterobacter Cloacae ◽  
Fecal Coliforms ◽  
Decarboxylase Activity ◽  
Histamine Concentration ◽  
And Control ◽  
Histidine Decarboxylase Activity

The histidine decarboxylase activity of Enterobacter cloacae S15/19 was studied during the production process of salchichón, a Spanish ripened sausage. Counts of fecal coliform and histidine decarboxylase bacteria decreased during the production process, showing a good correlation in both inoculated and control samples. In the samples inoculated with Enterobacter cloacae S15/19, fecal coliforms were undetectable the last day of the survey, while the population of histidine decarboxylase bacteria was over 2 log MPN/g. Despite the fact that inoculation with Enterobacter cloacae S15/19 increased histidine decarboxylase bacteria counts, no differences were observed in the histamine concentration reached, which was undetectable in most of the control and inoculated samples. In contrast, cadaverine concentration increased significantly (P &lt; 0.01) in the inoculated samples, suggesting that cadaverine could be used as a hygienic-quality indicator of the raw materials employed in sausage processing.

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