scholarly journals Application of FT-IR spectroscopy in detection of food hydrocolloids  in confectionery jellies and food supplements

2013 ◽  
Vol 19 (No. 2) ◽  
pp. 51-56 ◽  
Author(s):  
J. Čopíková ◽  
M. Černá ◽  
M. Novotná ◽  
J. Kaasová ◽  
A. Synytsya
Keyword(s):  
Ir Spectroscopy ◽  
Ir Spectra ◽  
Food Products ◽  
Food Supplements ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

FT-IR spectra of isolated high molecule fractions were measured and used for identification of food hydrocolloids in confectionery jellies and food supplements. The simple comparison of spectra of standards and samples proved that this technique is useful for the monitoring of food hydrocolloids in particular food products.

scholarly journals Evaluation of solubility and histological effect of 11-keto-β-Boswellic acid on the diabetic mice liver using FTIR-PCA analysis and microscopy

2021 ◽  
Author(s):  
I.S. Al-Amri ◽  
F. Mabood ◽  
I.T. Kadim ◽  
A.Y. Alkindi ◽  
A. Al-Harrasi ◽  
...  
Keyword(s):  
Ir Spectroscopy ◽  
Ir Spectra ◽  
Liver Tissue ◽  
Diabetic Control ◽  
Control Group ◽  
Diabetic Mice ◽  
Boswellic Acid ◽  
Ft Ir ◽  
Mice Liver ◽  
Ft Ir Spectroscopy

ABSTRACTThis study was designed to develop a rapid, sensitive, accurate, and inexpensive Fourier Transform Infrared Reflectance (FT-IR) Spectroscopy coupled with Principle Component Analysis (PCA) as a detection technique to evaluate the solubility of 11-Keto-β-Boswellic acid (KBA), from the gum resin extracted from the Omani frankincense, (Boswellia sacra) in the liver of STZ induced diabetic mice. This study also investigated the effect of KBA on the histological changes of hepatocytes of diabetic mice. Liver tissue samples from three groups of mice included normal control group, diabetic control group and diabetic group treated IP with KBA were scanned with FT-IR spectrophotometer in the reflection mode. FT-IR Spectra were collected in the wavenumber range from 400 to 4000cm-1 using ATR accessorry. The results of FT-IR Spectra were analyzed by using multivariate method Principle Component Analysis. The PCA score plot is an exploratory multivariate method indicated that there was a complete segregation among the three groups of liver samples based on change in variation of position of wavenumber in FT-IR spectra, which revealed that there is a clear effect of KBA solubility on treatments. The histological features showed an improvement in the liver tissues with normal structures of hepatocytes with exhibiting mild vacuolations in their cytoplasm. In conclusion, reflectance FT-IR spectroscopy coupled with PCA could be deployed as a new detection method for rapid, low cost and non-destructive method for evaluating of treatment effects in diseased liver tissue based on the solubility of KBA. Histological findings demonstrated the protective effective of KBA on improving the morphology of liver tissue in diabetic mice which resulted in complete recovery to the damage observed in diabetic control group.Summary StatementReflectance FT-IR spectroscopy coupled with PCA has been deployed as a new rapid, inexpensive and non-destructive detection method to examine the solubility of 11-keto-β-Boswellic acid (KBA) in streptozotocin (STZ) induced-diabetes mice liver tissue following intraperitoneal treatment. Moreover, microscopic study of liver tissue histopathology revealed that KBA has a protecting effect against STZ damage.

scholarly journals Classification of Five Kinds of Moss Plants with the Use of Fourier Transform Infrared Spectroscopy and Chemometrics

2011 ◽  
Vol 25 (6) ◽  
pp. 271-285 ◽  
Author(s):  
Tao Hu ◽  
Wen-Ying Jin ◽  
Cun-Gui Cheng
Keyword(s):  
Cluster Analysis ◽  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Ir Spectroscopy ◽  
Ir Spectra ◽  
Fourier Transform Infrared Spectroscopy ◽  
Moss Species ◽  
Ft Ir ◽  
And Cluster Analysis ◽  
Ft Ir Spectroscopy

Fourier transform infrared spectroscopy (FT-IR) with Horizontal Attenuated Total Reflectance (HATR) techniques is used to obtain the FT-IR spectra of five kinds of mosses, such asPtychomitrium dentatum(Mitt.) Jaeg.,Ptychomitrium polyphylloides(C. Muell.) Par.,Ptychomitrium sinense(Mitt.) Jaeg.,Macromitrium syntrichophyllumTher. Etp. Vard., andMacromitrium ferrieiCard. Sz Ther. Based on the comparison of the above mosses in the FT-IR spectra, the region ranging from 4000 to 650 cm−1was selected as the characteristic spectra for analysis. Principal component analysis (PCA) and cluster analysis are considered to identify the five moss species. Because they belong to the homogeneous plants, and have similar chemical components and close FT-IR spectroscopy, PCA and cluster analysis can only give a rough result of classification among the five moss species, Fourier self-deconvolution (FSD) and discrete wavelet transform (DWT) methods are used to enhance the differences between them. We use these methods for further study. Results show that it is an excellent method to use FT-IR spectroscopy combined with FSD and DWT to classify the different species in the same family. FT-IR spectroscopy combined with chemometrics, such as FSD and DWT, can be used as an effective tool in systematic research of bryophytes.

scholarly journals Criteria for standartization of probiotic components in functional food products

2018 ◽  
Vol 6 (2) ◽  
pp. 457-466
Author(s):  
Andrey Sukhikh ◽  
Andrey Sukhikh ◽  
Yuliya Zakharova ◽  
Yuliya Zakharova ◽  
Arseniy Yuzhalin ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Cell Wall ◽  
Ir Spectroscopy ◽  
Functional Food ◽  
Gel Permeation Chromatography ◽  
Food Products ◽  
Chemical Study ◽  
Bacterial Cells ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

The increasing volume of consumption of probiotics and functional food products requires determination of standardized criteria for cultures and their exometabolites used in functional products manufacturing. The study was aimed at developing criteria for the estimation and standardization of exometabolites and the colony-forming ability of probiotic strains for functional food production. The work included such microbiological and physicochemical methods as GC-MS, GPC, UV, and FT-IR-spectroscopy. Based on the results of the study, the comparative analysis of the microbiological properties of probiotic Bifidobacterium strains was provided, the fatty acid composition of the cell wall was described, and the physical and chemical study of the exopolymers produced by them was carried out. According to the data of FT-IR- spectroscopy, the characteristic features of the components of the cell wall of Bifidobacterium strains were established. Bifidobacteria form the unique composition of organophosphorus structures of lipoteichoic acids, which determines the adhesive ability of strains. The authors studied the molecular weight distribution of the samples of exometabolites isolated from the nutrient medium after the cultivation of bifidobacteria, under conditions of gel-permeation chromatography. The spectral (UV, FT-IR) characteristics of the produced metabolites and their chromatographic fractions were compared. The fatty acids of the Bifidobacterium cell membrane were analyzed using the GC/MS method. The fatty acids were extracted from bacterial cells with different hydrophobicity with a mixture of chloroform and hexane. It has been established that the hydrophobicity is determined by different contents of unsaturated and branched fatty acids in the bacterial membrane. Hydrophobic bifidobacteria are the only that contain the isopentadecane (isoC15:0) and methyl-tetradecanoic (13Me-C14:0) acids. With the mean hydrophobicity, a high content of the isopalmitic (isoC16:0) and stearic (C18:0) acids was established. Low-hydrophobic strains are characterized by a low content of monounsaturated fatty acids.

scholarly journals Results obtained by investigating saffron ussing FT-IR spectroscopy

2016 ◽  
Vol 73 (2) ◽  
pp. 238
Author(s):  
Luisa Andronie ◽  
Aurelia Coroian ◽  
Vioara Miresan ◽  
Ioana Pop ◽  
Camelia Raducu ◽  
...  
Keyword(s):  
Biological Activity ◽  
Ir Spectroscopy ◽  
Ir Spectra ◽  
Ir Spectrum ◽  
Research Work ◽  
Ft Ir ◽  
Pharmaceutical Properties ◽  
Ft Ir Spectroscopy

The biological activity and the pharmaceutical properties of plants are strongly dependent on their structure.The FT-IR spectra of saffron (commercial) have been obtained. The vibrational fundamentals from the IR spectrum, were analyzed  and assigned acoording to the available literature. In the present research work the genus saffron is selected because it is famous in wold as foods and also as medicine.

The Nature of Cation–Anion Interactions in Magnetic Ionic Liquids as Revealed Using High-Pressure Fourier Transform Infrared (FT-IR) Spectroscopy

2019 ◽  
Vol 73 (5) ◽  
pp. 511-519 ◽  
Author(s):  
Christopher M. Burba ◽  
Hai-Chou Chang
Keyword(s):  
High Pressure ◽  
Fourier Transform ◽  
Ionic Liquids ◽  
Ir Spectroscopy ◽  
Ir Spectra ◽  
Ambient Pressure ◽  
Vibrational Modes ◽  
Magnetic Ionic Liquids ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Magnetic ionic liquids are a group of magneto-responsive compounds that typically possess high ionic conductivities and low vapor pressures. In spite of the general interest in these materials, a number of questions concerning the fundamental interactions among the ions remain unanswered. We used vibrational spectroscopy to gain insight into the nature of these interactions. Intramolecular vibrational modes of the ions are quite sensitive to their local potential energy environments, which are ultimately defined by cation–anion coordination schemes present among the ions. Ambient pressure Fourier transform infrared (FT-IR) spectroscopy indicates comparable interaction motifs for 1-ethyl-3-methylimidazolium tetrachloroferrate(III), [emim]FeCl4, and 1-ethyl-3-methylimidazolium tetrabromoferrate(III), [emim]FeBr4, magnetic ionic liquids. However, the vibrational modes of [emim]FeCl4 generally occur at slightly higher frequencies than those of [emim]FeBr4. These differences reflect different interaction strengths between the [emim]+ cations and [Formula: see text] or [Formula: see text] anions. This conclusion is supported by gas-phase ab initio calculations of single [emim]FeCl4 and [emim]FeBr4 ion pairs that show longer C–H···Br–Fe interaction lengths compared to C–H···Cl–Fe. Although the IR spectra of [emim]FeCl4 and [emim]FeBr4 are comparable at ambient pressure, a different series of spectroscopic changes transpire when pressure is applied to these compounds. This suggests [emim]+ cations experience different types of interaction with the anions under high-pressure conditions. The pressure-dependent FT-IR spectra highlights the critical role ligands attached to the tetrahalogenoferrate(III) anions play in modulating cation–anion interactions in magnetic ionic liquids.

scholarly journals Application of FT-IR Spectroscopy for Fingerprinting ofZymomonas mobilisRespiratory Mutants

2012 ◽  
Vol 27 ◽  
pp. 581-585 ◽  
Author(s):  
M. Grube ◽  
R. Rutkis ◽  
M. Gavare ◽  
Z. Lasa ◽  
I. Strazdina ◽  
...  
Keyword(s):  
Ir Spectroscopy ◽  
Nucleic Acids ◽  
Ir Spectra ◽  
Parent Strain ◽  
Hierarchical Cluster ◽  
Knockout Mutant ◽  
Growth Environment ◽  
Ft Ir ◽  
Macromolecular Composition ◽  
Ft Ir Spectroscopy

Z. mobilisATCC 29191 and its respiratory knockout mutants,kat-, ndh-, cytB-,andcydB-, were grown under anaerobic and aerobic conditions. FT-IR spectroscopy was used to study the variations of the cell macromolecular composition. Quantitative analysis showed that the concentration ratios—nucleic acids to lipids, forZ. mobilisparent strain,kat-, ndh-, cytB-,andcydB-strains, clearly distinguishedZ. mobilisparent strain from its mutant derivatives and corresponded fairly well to the expected degree of biochemical similarity between the strains. Two different FT-IR-spectra hierarchical cluster analysis (HCA) methods were created to differentiateZ. mobilisparent strain and respiratory knockout mutant strains. HCA based on discriminative spectra ranges of carbohydrates, nucleic acids, and lipids allowed to evaluate the influence of growth environment (aeration, growth phase) on the macromolecular composition of cells and differentiate the strains. HCA based on IR spectra of inoculums, in a diagnostic region including the characteristic nucleic acid vibration modes, clearly discriminated the strains under study. Thus it was shown that FT-IR spectroscopy can distinguish various alterations ofZ. mobilisrespiratory metabolism by HCA of biomass spectra.

scholarly journals Infrared study and computational simulations of coronary arteries atherosclerotic lesions for early diagnosis of disease progression

2021 ◽  
Vol 42 (Supplement_1) ◽  
Author(s):  
I Mamarelis ◽  
C H R Mamareli ◽  
E Mylonas ◽  
V Mamareli ◽  
K Spilopoulos ◽  
...  
Keyword(s):  
Coronary Artery ◽  
Ir Spectroscopy ◽  
Ir Spectra ◽  
Mathematical Simulation ◽  
Disease Progression ◽  
Coronary Arteries ◽  
Coronary Artery Stenosis ◽  
Bypass Grafting ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Abstract Introduction Coronary artery atherosclerotic disease is the most common cardiovascular disease and bypass grafting surgery (CABG) is an effective treatment. However, the pathogenic mechanism of coronary arteries atherosclerosis and disease progression is not yet clear. Purpose The use of FT-IR spectroscopy, hyperspectral microscope and mathematical simulation models are some of the tools to predict the morphological and elasticity disorders in the vessel wall due to molecular structure changes. Methods Biopsies of atherosclerotic native coronary arteries from 54 patients (44–85 years), who underwent coronary endarterectomy during bypass grafting surgery (CABG), were examined ex vivo. The FT-IR-spectra were recorded with a Nicolet-6700 spectrometer. Morphological changes of atheromatic plaques were performed with SEM-EDX, Fei-Co. CytoVita-Olympus hyperspectral microscope was used to obtain the cells. Results FT-IR spectroscopy (Figure 1A) showed that the disease affects the protein folding, leading to amyloid formation (beta-sheets), lipid peroxidation and AGEs (Advanced Glycation end products) production. The detection of amorphous CaCO3 (1415 and 872 cm–1) deposits in high lipophilic regions was of high importance. Increased mineral concentration leads to increased formation of crystalline deposits, consisting of CaCO3, CaHPO4, Ca3(PO4)2 and inorganic hydroxyapatite, resulting in arterial stenosis. Hyperspectral images confirmed the formation of micelles (1) due to amyloidosis and calcified cells (2), in agreement with FT-IR, ImageJ analysis data. Mathematical simulation model based on finite element method (Figure 1E) showed that arterial wall damage and elasticity changes were not homogenous. This model provides the time of crystallization of the calcium salts, which play crucial role to stenosis. Conclusions FT-IR spectra showed that the formation of amorphous CaCO3 in the presence of Mg2+, in reach of oxidized lipids regions, inhibit the development of coronary artery stenosis. Excessive of Ca2+ efflux promotes the crystallinity of CaCO3 and Ca3(PO4)2 deposits, leading to the development of atherosclerotic plaques and coronary artery stenosis. Mathematical models approach in a much better way the progression of arterial atherosclerosis. FUNDunding Acknowledgement Type of funding sources: None. Figure 1

In situ FT-IR spectroscopy investigations of dimethyl carbonate synthesis: on the contribution of gas-phase species

RSC Advances ◽  
2016 ◽  
Vol 6 (21) ◽  
pp. 17288-17289 ◽  
Author(s):  
F. C. Meunier
Keyword(s):  
Ir Spectroscopy ◽  
Ir Spectra ◽  
Gas Phase ◽  
Dimethyl Carbonate ◽  
Ft Ir ◽  
Ft Ir Spectroscopy

Gas-phase contributions need to be subtracted from in situ IR spectra collected over solids to reveal the spectrum of adsorbates.

Sign in / Sign up

Export Citation Format

Share Document