scholarly journals Occurrence of Peronospora dianthicola on carnations in the Czech Republic

2012 ◽  
Vol 48 (No. 2) ◽  
pp. 72-73 ◽  
Author(s):  
I. Šafránková
Keyword(s):  
Czech Republic ◽  
Downy Mildew ◽  
The Czech Republic ◽  
Visual Symptoms ◽  
Leaf Spots ◽  
Microscopic Features ◽  
First Time ◽  
Downy Mildew Disease

A downy mildew disease was observed on leaves of Dianthus chinensis L. cv. Scarlet in Brno in June 2011. Characteristics of visual symptoms and microscopic features are described. The morphology of the fungus was typical of Peronospora dianthicola, conforming to the published description by Francis (1983). P. dianthicola causing leaf spots on Dianthus has been recorded for the first time in the Czech Republic.

scholarly journals Peronospora hariotii on Buddleja in the Czech Republic

2009 ◽  
Vol 45 (No. 1) ◽  
pp. 12-15
Author(s):  
I. Šafránková ◽  
J. Müller
Keyword(s):  
Czech Republic ◽  
Downy Mildew ◽  
Leaf Spot ◽  
Leaf Spot Disease ◽  
Sexual Stage ◽  
The Czech Republic ◽  
Spot Disease ◽  
Microscopic Features ◽  
Butterfly Bush ◽  
Downy Mildew Disease

A downy mildew was observed on <I>Buddleja × weyeriana</I> Weyer ex Rehd. (<I>B. davidii × globosa</I>) cv. Sungold in a nursery in the northern part of the Czech Republic in July 2008. This downy mildew disease of <I>Buddleja</I> (Butterfly Bush) had been unknown in the country so far. Characteristics of visual symptoms and microscopic features are described. The morphology of the fungus was typical for <I>Peronospora hariotii</I>, conforming to the published description by GÄUMANN (1919). The sexual stage was not found. This is the first report of <I>Peronospora hariotii </I>causing leaf spot disease on <I>Buddleja</I> in the Czech Republic.

scholarly journals Investigator Initiated Clinical Trials (IICTs): A Systematic Search in Registries to Compare the Czech Republic and Portugal in Terms of Funding Policies and Scientific Outcomes

2021 ◽  
Author(s):  
C. Madeira ◽  
L. Hořavová ◽  
F. dos Santos ◽  
J. R. Batuca ◽  
K. Nebeska ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Czech Republic ◽  
Clinical Research ◽  
Systematic Search ◽  
European Countries ◽  
The Czech Republic ◽  
Research Outcomes ◽  
Funding Policies ◽  
Funding Agencies ◽  
First Time

Abstract Objectives Clinical trials provide one of the highest levels of evidence to support medical practice. Investigator initiated clinical trials (IICTs) answer relevant questions in clinical practice that may not be addressed by industry. For the first time, two European Countries are compared in terms of IICTs, respective funders and publications, envisaging to inspire others to use similar indicators to assess clinical research outcomes. Methods A retrospective systematic search of registered IICTs from 2004 to 2017, using four clinical trials registries was carried out in two European countries with similar population, GDP, HDI and medical schools but with different governmental models to fund clinical research. Each IICT was screened for sponsors, funders, type of intervention and associated publications, once completed. Results IICTs involving the Czech Republic and Portugal were n = 439 (42% with hospitals as sponsors) and n = 328 (47% with universities as sponsors), respectively. The Czech Republic and Portuguese funding agencies supported respectively 61 and 27 IICTs. Among these, trials with medicinal products represent 52% in Czech Republic and 4% in Portugal. In the first, a higher percentage of IICTs’ publications in high impact factor journals with national investigators as authors was observed, when compared to Portugal (75% vs 15%). Conclusion The better performance in clinical research by Czech Republic might be related to the existence of specific and periodic funding for clinical research, although further data are still needed to confirm this relationship. In upcoming years, the indicators used herein might be useful to tracking clinical research outcomes in these and other European countries.

scholarly journals First report of Cherry virus F infecting Japanese plum in Korea

Plant Disease ◽  
2020 ◽  
Author(s):  
Yeonhwa Jo ◽  
Hoseong Choi ◽  
Jin Kyong Cho ◽  
Won Kyong Cho
Keyword(s):  
Czech Republic ◽  
High Throughput Sequencing ◽  
Prunus Avium ◽  
De Novo ◽  
Protein Database ◽  
Specific Primers ◽  
The Czech Republic ◽  
First Report ◽  
Japanese Plum ◽  
Leaf Spots

Cherry virus F (CVF) is a tentative member of the genus Fabavirus in the family Secoviridae, consisting of two RNA segments (Koloniuk et al. 2018). To date, CVF has been documented in only sweet cherry (Prunus avium) in the Czech Republic (Koloniuk et al. 2018), Canada, and Greece. In May 2014, we collected leaf samples from four symptomatic (leaf spots and dapple fruits) and two asymptomatic Japanese plum cultivars (Sun and Gadam) grown in an orchard in Hoengseong, South Korea, to identify viruses and viroids infecting plum trees. Total RNA from individual plum trees was extracted using two commercial kits: Fruit-mate for RNA Purification Kit (Takara, Shiga, Japan) and RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). We generated six mRNA libraries from the six different plum cultivars for RNA-sequencing using the TruSeq RNA Library Preparation Kit v2 (Illumina, CA, U.S.A.) as described previously (Jo et al. 2017). The mRNA libraries were paired-end (2 X 100 bp) sequenced with a HiSeq 2000 system (Macrogen, Seoul, Korea). The raw sequence reads were de novo assembled by Trinity program v. 2.8.6, with default parameters (Haas et al. 2013). The assembled contigs were subjected to BLASTX search against the non-redundant protein database in NCBI. Of the two asymptomatic cultivars, the transcriptome of asymptomatic plum cv. Gadam contained five contigs specific to CVF. Two and three contigs were specific to CVF RNA1 (2,571 reads, coverage 42.15%) and RNA2 (2,025 reads, coverage 53.04%), respectively. The size of these five contigs ranged from 241 to 5,986 bp. Contigs of 5,986 and 3,867 bp in length, referred to as CVF isolate Gadam RNA1 (GenBank MN896996) and RNA2 (GenBank MN896995), respectively, were subjected to BLASTP search against NCBI’s non-redundant protein database. The results showed that the polyprotein sequences of RNA1 and RNA2 shared 95.3% and 93.11% amino acid identities with isolates SwC-H_1a from the Czech Republic (GenBank acc. no. AWB36326) and Stac-3B_c8 from Canada (AZZ10055), respectively. To confirm the infection of CVF in cv. Gadam, RT-PCR was conducted using CVF RNA1-specific primers designed based on the CVF reference genome sequences (MH998210 and MH998216), including 5’-CCACCAAATAGGCAAGAGGTCAC-3’ (position 3190–3212) and 5’-CACAATCACCATCAATGGTCTCTGC-3’ (position 3742–3766), and CVF RNA2-specific primers, including 5’-CTGCTTTATGATGCTAGACATCAAGATG-3’ (position 1015–1042) and 5’-ACAATAGGCATGCTCATCTCAACCTC-3’ (position 1594–1619). We amplified 577-bp RNA1-specific and 605-bp RNA2-specific amplicons that were cloned and then performed Sanger sequencing. Sequencing of the cloned amplicons for isolate Gadam RNA1 (GenBank MN896993) and RNA2 (GenBank MN896994) revealed values of 99.48% and 99.17% nucleotide identity to that of RNA1 and RNA2 determined by high-throughput sequencing, respectively. Additionally, we tested five plants for each of the six plum cultivars grown in the same orchard. The detection of CVF was carried out through PCR using the primers and protocol described above. Of the 30 trees, CVF was detected in three trees of cv. Gadam by both primer pairs. To our knowledge, this is the first report of CVF infecting Japanese plum and the first report of the virus in Korea. However, its prevalence in other Prunus species, including apricot, European plum, and peach, should be further elucidated.

scholarly journals New records of Anthomyiidae (Diptera) from the Czech Republic and Slovakia

2015 ◽  
Vol 64 (2) ◽  
pp. 151-154 ◽  
Author(s):  
Olga Komzáková ◽  
Verner Michelsen
Keyword(s):  
Czech Republic ◽  
New Records ◽  
The Czech Republic ◽  
The Family ◽  
First Time

Abstract Althogether 17 species of the family Anthomyiidae (Diptera) are recorded from the Czech Republic (or Moravia and Bohemia) (16 species) and Slovakia (1 species) for the first time. The most interesting findings are Delia dovreensis Ringdahl 1954 and northamerican species Pegomyia bifurcata Griffiths 1983.

scholarly journals The First Report of Downy Mildew Caused by Peronospora belbahrii on Sweet Basil in Greenhouses in the Czech Republic

Plant Disease ◽  
2014 ◽  
Vol 98 (11) ◽  
pp. 1579-1579 ◽  
Author(s):  
I. Šafránková ◽  
L. Holková
Keyword(s):  
Czech Republic ◽  
Downy Mildew ◽  
Width Ratio ◽  
Specific Sequence ◽  
The Czech Republic ◽  
First Report ◽  
Sweet Basil ◽  
Potted Plants ◽  
Length Width ◽  
Necrotic Spots

Sweet basil (Ocimum basilicum L.) is an aromatic plant that is cultivated as a pot plant in greenhouses or in fields in the Czech Republic. The plants are intended for direct consumption or for drying. In April of 2012, the first large chlorotic from the middle necrotic spots occurred gradually on leaves of pot plants O. basilicum cv. Genovese in greenhouses in Central Bohemia. The characteristic gray to brown furry growth of downy mildew appeared on abaxial surfaces of leaves in the place of chlorotic spots within 3 to 4 days. The infested leaves fell off in the late stages of pathogenesis. The infestation gradually manifested itself in ever-younger plants and in July, cotyledons and possibly the first true leaves were already heavily infected and damaged and these plants rapidly died. The plant damage reached 80 to 100%, so it was necessary to stop growing the plants in the greenhouse at the end of July. The causal agent was isolated and identified as Peronospora belbahrii Thines by means of morphological and molecular characters (2,3). Conidiophores were hyaline, straight, monopodial, 280 to 460 μm, branched three to five times, ended with two slightly curved branchlets with a single conidia on each branchled tip. The longer branchlets measured 13 to 24 μm (average 18.2 μm), the shorter one 4 to 15 μm (average 9.7 μm). Conidia were rounded or slightly ovoid, from brownish to dark brownish, measured 22 to 31 × 20 to 28 μm (length/width ratio 1.2). A pathogen-specific sequence was detected with the help of the pathogen ITS rDNA specific primers in symptomatic leaves (1). DNA from plant tissues was isolated using the DNeasy plant Mini Kit (Qiagen, Germany) following the standard protocol. PCR was performed using KAPA2G Robust HotStar kit (Kapa Biosystems, United States) according to the conditions recommended in Belbahri et al. (1). The specific products were visualized by electrophoresis through 1.5% agarose gels. Leaves of 20-day-old potted plants O. basilicum ‘Genovese’ were inoculated by spraying with 5 × 105 conidia/ml of the pathogen. Each pot contained 10 plants. Sterilized distilled water was applied to control plants. Plants were covered with polyethylene bags during the entire incubation period to maintain high humidity, and kept at a temperature of 22 to 24°C. Typical disease symptoms appeared on leaves 5 to 9 days after inoculation. Control plants were symptomless. P. belbahrii was re-isolated from the lesions of inoculated plants, thus fulfilling Koch's postulates. Downy mildew on sweet basil was reported in countries in Africa, Europe, and South and North America (4). To our knowledge, this is the first report of downy mildew on sweet basil in the Czech Republic. References: (1) L. Belbahri et al. Mycol. Res. 109:1276, 2005. (2) Y.-J. Choi et al. Mycol. Res. 113:1340, 2009. (3) M. Thines et al. Mycol. Res. 113:532, 2009. (4) C. A. Wyenandt et al. HortScience 45:1416, 2010.

scholarly journals Synanthedon mesiaeformis (Herrich-Schäffer) new to the Czech Republic and to Spain (Lepidoptera: Sesiidae)

2008 ◽  
Vol 56 (5) ◽  
pp. 141-146 ◽  
Author(s):  
Zdeněk Laštůvka ◽  
Aleš Laštůvka
Keyword(s):  
Czech Republic ◽  
Close Contact ◽  
Morphological Characters ◽  
Habitat Requirements ◽  
Specific Population ◽  
The Czech Republic ◽  
Southern France ◽  
History Of ◽  
First Time ◽  
The Town

Synanthedon mesiaeformis (Herrich-Schäffer, 1846) has been found in the Czech Republic and in Spain for the first time. The species was found in the south-easternmost part of the Czech Republic, near the town of Břeclav (faunistic quadrat 7267) in May 2008. The holes and pupae were found only in one, solitary growing group of trees about 20 years old. This finding place lies at a distance of more than 250 km from the localities in SW Hungary and about 550 km from the localities in eastern Poland. In June 2008, the species was found also in alders growing in the flat river alluvium on gravel sands between La Jonquera and Figueres in northern Catalonia. This locality is in a close contact with the fin­ding places near Perpignan and Beziers in southern France. The diagnostic morphological characters and bionomics of this species are briefly summarized and figured. The history of its distribution research is recapitulated and the causes of its disjunct range are discussed as follows. The present disjunct range represents a residual of the former distribution over the warmer and moister postglacial period; landscape modifications and elimination of solitary alder trees as „weeds“ from the 18th up to the mid-20th century in large areas of Europe; narrow and partly unknown habitat requirements and specific population ethology; an insufficient level of faunistic investigations in several parts of sou­thern and eastern Europe.

scholarly journals Dothistroma needle blight Mycosphaerella pini E. Rostrup, a new quarantine pathogen of pines in the CR

2012 ◽  
Vol 50 (No. 7) ◽  
pp. 319-326 ◽  
Author(s):  
L. Jankovský ◽  
M. Bednářová ◽  
D. Palovčíková
Keyword(s):  
Czech Republic ◽  
Host Species ◽  
Pinus Nigra ◽  
Climatic Conditions ◽  
The Czech Republic ◽  
Picea Pungens ◽  
Quarantine Pathogen ◽  
Dothistroma Needle Blight ◽  
First Time ◽  
Needle Blight

Dothistroma needle blight caused by Mycosphaerella pini E. Rostrup was observed for the first time in the Czech Republic on an imported Pinus nigra Arnold in 1999. In 2000, it was also found in the open planting. During three years, it became an important pathogen of pines in the Czech Republic. Its occurrence was noticed in more than 50 localities, above all in the region of Moravia and Silesia and eastern Bohemia. In total, it was found on 10 species of pine (P. nigra Arnold, P.&nbsp;banksiana Lamb., <br />P. contorta Loudon, P. mugo Turra, P.&nbsp;leucodermis Ant., P. sylvestris L., P. cembra L., P. aristata Engelm., P.&nbsp;ponderosa P. et C.&nbsp;Lawson and P. jeffreyi Grev. et Balf.). Also Picea pungens &nbsp;Engelm. was noticed as a host species. In the Czech Republic, Pinus nigra is the most frequent host species of M. pini (80% localities) followed by Pinus mugo (27% localities). On Scots pine P. sylvestris, M. pini was noticed at two localities. The critical period for infection is in the Czech Republic from the second half of May until the end of June. The incubation period lasts about 2&ndash;4 months depending on climatic conditions. The first symptoms on the needles infected in the current year appear in August being clearly expressed from September to November.In the CR, Dothistroma needle blight spread probably with infected planting stock obtained from import at the end of the 80s and at the beginning of the 90s.

scholarly journals First records of Leptoglossus occidentalisdemann, 1910 (Heteroptera: Pentatomorpha: Coreidae) in the Czech Republic

2008 ◽  
Vol 43 (No. 4) ◽  
pp. 165-168 ◽  
Author(s):  
J. Beránek
Keyword(s):  
Czech Republic ◽  
North America ◽  
Insect Species ◽  
The Czech Republic ◽  
Coniferous Trees ◽  
Leptoglossus Occidentalis ◽  
Alien Insect ◽  
First Time

In the autumn of 2006 and summer 2007 the coreid species <I>Leptoglossus occidentalis</I> Heidemann, 1910 was detected in the Czech Republic for the first time as a new alien insect species. It is native to North America, where it causes important seed losses on coniferous trees, especially pines. From Europe it is known since the end of the last century, when it was introduced to Italy. Its repeated finding could indicate a vital population of this species in the Czech Republic, not only introduced individuals.

scholarly journals Occurrence of rust disease caused by Puccinia oxalidis on Oxalis triangularis in the Czech Republic – Short Communication 

2014 ◽  
Vol 50 (No. 1) ◽  
pp. 17-18
Author(s):  
I. Šafránková
Keyword(s):  
Czech Republic ◽  
Leaf Spot ◽  
Short Communication ◽  
Rust Disease ◽  
The Czech Republic ◽  
First Report ◽  
Microscopic Features

This is the first report of&nbsp;Puccinia oxalidis&nbsp;causing leaf spot diseases on ornamental&nbsp;Oxalis triangularis&nbsp;subsp.&nbsp;papilionaceae&nbsp;cv. Atropurpurea in Moravia, Czech Republic. The macroscopic symptoms and microscopic features are described.

scholarly journals Contribution to identify the causal gents of Dutch elm disease in the Czech Republic

2008 ◽  
Vol 43 (No. 4) ◽  
pp. 142-145 ◽  
Author(s):  
M. Dvořák ◽  
M. Tomšovský ◽  
L. Jankovský ◽  
D. Novotný
Keyword(s):  
Czech Republic ◽  
North America ◽  
Molecular Biology ◽  
The Other ◽  
Dutch Elm Disease ◽  
The Czech Republic ◽  
Ophiostoma Ulmi ◽  
Other Hand ◽  
Subspecies Level ◽  
First Time

This study provides new data on Dutch elm disease in the Czech Republic. <I>Ophiostoma novo-ulmi</I> is reported for the first time in the area of the Czech Republic, as well as both subspecies ssp. <I>novo-ulmi</I> (indigenous in the area of the Ukraine and Moldavia), and ssp. <I>Americana</I> indigenous in North America. The majority of the recorded strains belonged to <I>O. n.-u.</I> ssp. <I>novo-ulmi</I>, while <I>O. n.-u.</I> ssp. <I>Americana</I> and hybrids of these two subspecies were found less frequently. On the other hand, <I>Ophiostoma ulmi</I> was not found at all in the investigated samples. Identification on the subspecies level was performed by methods of molecular biology, i.e. PCR and RFLP of gene regions<I> cu</I> and <I>col1</I>.

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