scholarly journals Triticeae Genebank vollection as a source of resistance to fungal diseases

2012 ◽  
Vol 41 (Special Issue) ◽  
pp. 189-192
Author(s):  
V. Holubec ◽  
V. Dumalasová ◽  
A. Hanzalová
Keyword(s):  
Full Text ◽  
Fungal Diseases ◽  
Resistance To Fungal Diseases

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EFFECTIVENESS OF INFECTIOUS BACKGROUNDS IN EVALUATING OAT FOR RESISTANCE TO FUNGAL DISEASES

2020 ◽  
pp. 10-13
Author(s):  
O.A. Zhuikova ◽  
◽  
T.P. Gradoboeva ◽  
G.A. Batalova ◽  
◽  
...  
Keyword(s):  
Fungal Diseases ◽  
Resistance To Fungal Diseases

scholarly journals Sources of resistance to fungal diseases for selection of spring soft wheat

2020 ◽  
pp. 45-49
Author(s):  
Tatyana Yuryevna Taranova ◽  
Alexander Ivanovich Kincharov ◽  
Elena Anatolyevna Demina ◽  
Olga Sergeevna Mullayanova
Keyword(s):  
Powdery Mildew ◽  
Leaf Rust ◽  
Climatic Conditions ◽  
Fungal Diseases ◽  
Soft Wheat ◽  
Sources Of Resistance ◽  
Middle Volga Region ◽  
New Genes ◽  
Spring Soft Wheat ◽  
Resistance To Fungal Diseases

For breeding to create varieties resistant to fungal diseases, it is effective to work in the search for new genes of resistance to pathogens, as well as to constantly update the gene pool of spring wheat. The researches were conducted in 2014-2019 in the Samara region. The object of study was 352 collection samples of spring soft wheat of various ecological and geographical groups. The assessment of samples for resistance to fungal pathogens was carried out against a natural infectious background, including in years with a strong development of certain diseases (powdery mildew – 2014, brown rust – 2016, 2017). Accounting of plant damage by fungal diseases was carried out according to generally accepted phytopathological methods. Since 2000, the laboratory has studied and developed more than one thousand collection samples, formed working characteristic collections of samples for resistance to leaf rust (142), powdery mildew (185) and with complex resistance to these diseases (100). According to the results of six-year studies, collection samples with complex resistance to leaf rust and powdery mildew were identified: Kinelskaya yubileynaya, Erythrospermum 4112, Erythrospermum 4143, Erythrospermum 4144, Erythrospermum 4146, Erythrospermum 4147, Lutescens 6045/7, Erythrospermum 6310/10-63 (Kinel), Khutoryanka (Tambov), Ulyanovskaya 105, Yaritsa (Ulyanovsk), Tulaykovskaya 108 (Bezenchuk), Sigma 2 (Omsk), KVS Aquilon (Germany). Relatively resistant to root rot pathogens samples were noted: Kinelskaya 2010, Lutescens 3960, Erythrospermum 4112, Erythrospermum 4171, Lutescens 4394 (Kinel). The selected varieties and breeding lines are recommended by us as sources of resistance to fungal diseases for use in breeding programs of the middle Volga region and research sites with similar agro-climatic conditions.

scholarly journals ASSESSMENT OF THE INTEGRATED CHITINASE GENE STABILITY IN WHEAT LINES AFTER BIOBALLISTIC TRANSFORMATION

REPORTS ◽  
2020 ◽  
Vol 6 (334) ◽  
pp. 5-13
Author(s):  
N.P. Malakhova ◽  
◽  
Y.A. Skiba ◽  
E.R. Maltseva ◽  
G.A. Iskakova ◽  
...  
Keyword(s):  
Plant Immunity ◽  
Dna Amplification ◽  
Chitinase Gene ◽  
Fungal Diseases ◽  
Pr Proteins ◽  
Pcr Analysis ◽  
Soil Conditions ◽  
Wheat Lines ◽  
Resistance To Fungal Diseases ◽  
Regenerant Plants

The synthesis of PR-proteins (pathogenesis related proteins), the most studied of which are chitinases and β-1,3-glucanases, occurs in response to infection with pathogens in plants. Information about the exact role of individual PR proteins within plant immunity makes it possible to use certain specific antifungal proteins for the development of transgenic plants with increased resistance to fungal diseases. At the same time, it is important not only to obtain a plant with the desired trait, but also to fix in it the stable expression of the transferred gene and the inheritance of the acquired trait in generations. Herein we have studied the stability of the chitinase gene insertion in T1, T2 and T3 generations of transformed wheat lines obtained by the method of cis-gene transfer. Primary transformed regenerant plants were obtained as a result of bioballistic transformation of the chitinase gene into immature wheat germ of Saratovskaya 29 and Kazakhstanskaya 19 varieties. Following screening of regenerant plants by PCR for the presence of the target gene made it possible to select 6 lines presumably carrying the insert based on variety Saratovskaya 29 and 2 lines based on variety Kazakhstanskaya 19. The seed material of the selected regenerant plants was cultivated in soil conditions and the seeds of the T1 generation were obtained. DNA amplification of 8 selected lines St-29№25, St -29№43, St-29№44, St-29№33, St-29№26, St-29№35, Кz-19№1, Кz-19№2 with specific primers revealed insert-carrying lines, partially cleaved lines and lines with a high degree of insert instability. According to the results of the T2 and T3 generations PCR analysis, a complete absence of insertion in the St-29№35 and St-29№33 lines was revealed, a partial cleavage of the trait in the St-29№43 and Kz-19№2 lines was revealed, and the stable inheritance of the chitinase gene in four lines St-29№25, St-29№44, St-29№26 and Kz-19№1.was confirmed. These lines were selected as promising for in-depth study of their resistance to fungal diseases and further replication.

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