scholarly journals Impact of Piper betle leaf extract on grape downy mildew: effects of combining 4-allylpyrocatechol with eugenol, α-pinene or β-pinene

2018 ◽  
Vol 55 (No. 1) ◽  
pp. 23-30
Author(s):  
Aoki Yoshinao ◽  
Trung Nguyen Van ◽  
Suzuki Shunji
Keyword(s):  
Downy Mildew ◽  
Methanol Extract ◽  
Inhibitory Effect ◽  
Mass Spectrometry Analysis ◽  
Piper Betle ◽  
Gas Chromatograph ◽  
Inhibitory Effects ◽  
Spectrometry Analysis ◽  
Tandem Mass Spectrometry Analysis ◽  
Grape Downy Mildew

Methanol extract of Piper betle leaves exhibited an inhibitory effect on grape downy mildew. This extract might contain more than two compounds which have different polarities that suppress grape downy mildew. Gas chromatograph-tandem mass spectrometry analysis identified 4-allylpyrocatechol, eugenol, α-pinene, and β-pinene in the methanol extract. Neither of the compounds suppressed grape downy mildew by single treatment. On the other hand, treatment with a combination of 4-allylpyrocatechol with eugenol, α-pinene or β-pinene enhanced the inhibitory effects on grape downy mildew and perfectly suppressed it. The complex extracted from P. betle leaves may be used in organic agriculture as an alternative to chemical fungicides in viticulture.

scholarly journals Development and method validation for determination of 54 pesticides in Okra by LC-MS/MS analysis

2020 ◽  
Vol 11 (1) ◽  
pp. 985-992
Author(s):  
Hymavati Muppalla ◽  
Kiranmayi Peddi
Keyword(s):  
European Union ◽  
Pesticide Residues ◽  
High Performance ◽  
Limit Of Detection ◽  
Extraction Procedure ◽  
Mass Spectrometry Analysis ◽  
Limit Of Quantification ◽  
Spectrometry Analysis ◽  
Tandem Mass Spectrometry Analysis

The presence of pesticide residues in primary and derived agricultural products raises serious health concerns for consumers across the globe. The aim of the present study was to assess the level of pesticide residues in Okra in India. A multi-residue method for the quantification of fifty-four pesticides in okra is described in this work. The present study employed a modified quick, easy cheap, effective rugged and safe (QuEChERS) extraction procedure followed by UHPLC-MS/MS (Ultra-High-Performance Liquid Chromatography coupled to Tandem Mass Spectrometry) analysis. Validation of the method was according to the guidelines given by European Union SANCO/12571/2013. The levels of validation were 10.0, 50.0 and 100 µg kg-1. The following parameters such as linearity, the limit of detection (LOD) (nearer to 0.005 mg kg-1) and limit of quantification (LOQ) (nearer to 0.01 mg kg-1) were set to be acceptable. The trueness of the method for 54 pesticides in all Okra commodities was between 80-110% with satisfactory repeatability and within-run reproducibility except for the pesticide residues such as Thiamethoxam and Fenamidone. The measurement of uncertainty for each of the pesticide was below 50% and was estimated to be in the range of 5.37% - 10.71%, which meets the criteria established in the SANCO/12571/2013 document (European Union, 2013). This method is concluded to be applicable for the determination of pesticide residues in Okra.

Metabolic profile of Yi-Xin-Shu capsule in rat by ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry analysis

RSC Advances ◽  
2015 ◽  
Vol 5 (98) ◽  
pp. 80583-80590 ◽  
Author(s):  
Hongping Wang ◽  
Chang Chen ◽  
Yan Liu ◽  
Xiaowei Yang ◽  
Hongbin Xiao
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Metabolic Profile ◽  
Ultra Performance Liquid Chromatography ◽  
Mass Spectrometry Analysis ◽  
Tandem Mass ◽  
Spectrometry Analysis ◽  
Tandem Mass Spectrometry Analysis ◽  
Metabolite Profiles

In this study, the metabolite profiles of YXS in different biofluids of rats were investigated.

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