scholarly journals Determination of the cluster of wheat rust resistance genes Yr17, Lr37, and Sr38 by a molecular marker

2012 ◽  
Vol 38 (No. 2) ◽  
pp. 41-45 ◽  
Author(s):  
M. Ambrozková ◽  
F. Dedryver ◽  
V. Dumalasová ◽  
A. Hanzalová ◽  
P. Bartoš
Keyword(s):  
Czech Republic ◽  
Molecular Marker ◽  
Rust Resistance ◽  
Yellow Rust ◽  
Field Trials ◽  
Aegilops Ventricosa ◽  
The Czech Republic ◽  
Wheat Rust ◽  
Rust Resistance Genes

A translocation from Aegilops ventricosa carrying genes Yr17, Lr37 and Sr38 was verified in cultivars Hussar, Eureka, Torfrida, Renan, Rendezvous, Rapier and Brigadier by the molecular marker SCARSC-Y15. Of the cultivars recently registered in the Czech Republic, only the western European cultivars Corsaire, Apache, Complet and Bill possessed the translocation carrying Yr17, Lr37 and Sr38. Cultivars Corsaire, Apache, Bill and Complet were highly or medium resistant to yellow rust and leaf rust in 1999–2001 field trials at Prague-Ruzyně, although virulence to Yr17 was found in the 2001 virulence survey also in the Czech Republic. Cultivars Corsaire, Apache and Bill displayed an above average resistance to stem rust, whereas Complet was susceptible.

scholarly journals Presence of a Translocation from Aegilops ventricosa in Wheat Cultivars Registered in the Czech Republic

2011 ◽  
Vol 40 (No. 2) ◽  
pp. 31-35 ◽  
Author(s):  
P. Bartoš ◽  
J. Ovesná ◽  
A. Hanzalová ◽  
J. Chrpová ◽  
V. Dumalasová ◽  
...  
Keyword(s):  
Czech Republic ◽  
Leaf Rust ◽  
Rust Resistance ◽  
Leaf Rust Resistance ◽  
Wheat Cultivars ◽  
Pcr Marker ◽  
Aegilops Ventricosa ◽  
The Czech Republic ◽  
Rust Resistance Genes ◽  
Leaf Rust Resistance Genes

The presence of a translocation from Aegilops ventricosa carrying the genes for rust resistance Yr17, Lr37 and Sr38 was analysed in recently registered, mostly western European wheat cultivars in the Czech Republic. By means of a PCR marker the presence of the translocation was determined in cvs. Bill, Clarus, Clever, Corsaire, Rapsodia, and in the Czech cv. Rheia. Novel are the data for cvs. Rapsodia, Clarus and Rheia. Infection tests indicated the presence of additional leaf rust resistance genes in cultivars with the translocation, except in cv. Rheia. Segregating progenies of six crosses between cv. Renan possessing Lr37and different cultivars susceptible to leaf rust were tested for the presence of the translocation with Yr17, Lr37 and Sr38 by an infection test as well as by a molecular marker. High coincidence between the results from infection tests and those by the marker has been proved.  

scholarly journals Dynamics of the Degradation of Acetyl-CoA Carboxylase Herbicides in Vegetables

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 405
Author(s):  
Miroslav Jursík ◽  
Kateřina Hamouzová ◽  
Jana Hajšlová
Keyword(s):  
Linear Models ◽  
Growing Season ◽  
Field Trials ◽  
Baby Food ◽  
Vegetable Production ◽  
The Czech Republic ◽  
Herbicide Residues ◽  
Harvest Intervals ◽  
Small Plot

(1) Background: Aryloxyphenoxy-propionates and cyclohexanediones are herbicides most widely used in dicot crops worldwide. The main objective of the study was to determine the dynamics of herbicide residues in carrot, lettuce, cauliflower, and onion in order to suggest a low level of residues in harvested vegetables. (2) Methods: Small plot field trials were carried out in four vegetables in the Czech Republic. The samples of vegetables were collected continuously during the growing season. Multiresidue methods for the determination of herbicide residues by LC-MS/MS were used. Non-linear models of degradation of individual herbicides in vegetables were calculated using the exponential decay formula. Action GAP pre-harvest intervals for the 25% and 50% maximum residue limit (MRL) and 10 µg kg−1 limit (baby food) were established for all tested herbicides. (3) Results: The degradation dynamics of fluazifop in carrot, onion, and cauliflower was significantly slower compared to quizalofop and haloxyfop. The highest amount (2796 µg kg−1) of fluazifop residues was detected in cauliflower 11 days after application. No residue of propaquizafop and cycloxydim was detected in any vegetable samples. (4) Conclusions: Aryloxyphenoxy-propionate herbicide (except propaquizafop) could contaminate vegetables easily, especially vegetables with a short growing season. Vegetables treated with fluazifop are not suitable for baby food. Lettuce and cauliflower treated by quizalofop are not suitable for baby food, but in onion and carrot, quizalofop could be used. Propaquizafop and cycloxydim are prospective herbicides for non-residual (baby food) vegetable production.

A Comparative Analysis of the Power of Selected European Countries. The Economic Determinants

2021 ◽  
Vol 37 (37) ◽  
pp. 22-42
Author(s):  
Alicja Paluch ◽  
Henryk Spustek
Keyword(s):  
Czech Republic ◽  
Comparative Analysis ◽  
European Countries ◽  
Economic Power ◽  
Strongly Correlated ◽  
The Czech Republic ◽  
Independent Variables ◽  
Economic Sphere ◽  
National Power

The ever-increasing need for in-depth analysis and quantification of the national power, in particular ‘hard’ and ‘soft’ power-generating factors as well as difficulties in identifying a comprehensive and effective method for scientific determination of the national power, have given rise to research in the indicated scientific issues within this article. The presented considerations aim to define the assumptions for a descriptive sub-model that would enable a comparison of Poland’s power in the economic sphere (which is a component of the non-military sphere) with the power of selected European countries. The research hypothesis is that, among the variety of descriptive variables in the economic sphere of the national power, there is a subset of mutually independent variables, at the same time strongly correlated with the national power, which make it possible to define assumptions for the sub-model of the national power. The steps of the research procedure were carried out using the method of system analysis (multi-criteria comparative analysis) and statistical analysis. The research activities undertaken have shown that the factors that are strongly correlated with the national power in the economic area of the European countries adopted for the analysis are: dynamics of industrial production, private sector credit flows and economic freedom index. The comparative analysis carried out demonstrates that the greatest increase in the economic power in the analysed period took place in Germany (0.68). Slightly smaller growth was recorded in the Czech Republic (0.62) and Poland (0.60), while the lowest value of increase was in Romania (0.23). The conducted qualitative comparative analysis of the economic power of selected European countries allowed to conclude that the independent variables identified are crucial for the formation of the economic power of the analysed countries. At the same time, a fairly strong position of the Czech Republic and Poland in relation to the economic power of Germany was found. The performed quantification of the economic power of the European countries provides a basis for the correct determination of changes in the power distribution of political units, assessment of the power and resources held by the state.

scholarly journals Evaluation of gooseberry cultivars resistance to the American gooseberry powdery mildew Sphaerotheca mors uvae Schwein and its chemical control

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 583-587
Author(s):  
M. Vaverka ◽  
S. Vaverka
Keyword(s):  
Biological Activity ◽  
Czech Republic ◽  
Powdery Mildew ◽  
Chemical Control ◽  
Biological Effect ◽  
Field Trials ◽  
The Czech Republic ◽  
Biological Efficacy ◽  
Extensive Field ◽  
Susceptibility Evaluation

In the course of 1993–2001 extensive field trials were carried out to evaluate the resistance (susceptibility) level of 34 gooseberry cultivars to the American gooseberry powdery mildew Sphaerotheca mors uvae Schwein. Cultivars originated from the Czech Republic and from other European countries. Each tested cultivar had 7 trees (5–10 years old). Beside the resistance (susceptibility) evaluation, biological efficacy of 9 fungicides using EPPO methods has been checked at the same number of cultivars and at the same number of gooseberry trees. Highly significant differences of resistance or susceptibility were observed among gooseberry varieties. Analogical results (differences in biological activity of fungicides) have been attained in the course of chemical treatment. 18 cultivars has been classified as low resistant, 12 cultivars as moderate resistant and 4 cultivars as high resistant. None of the tested fungicides proved perfect biological efficacy (100% healthy berries). 4 of them proved high biological effect (more than 90% healthy berries), 3 proved low biological activity (less than 75% healthy berries) and 2 proved moderate biological activity (75–90% healthy berries).

scholarly journals Preliminary Study of Sars-Cov-2 Occurrence in Wastewater in the Czech Republic

2020 ◽  
Vol 17 (15) ◽  
pp. 5508 ◽  
Author(s):  
Hana Mlejnkova ◽  
Katerina Sovova ◽  
Petra Vasickova ◽  
Vera Ocenaskova ◽  
Lucie Jasikova ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Wastewater Treatment ◽  
Czech Republic ◽  
Wastewater Treatment Plants ◽  
Chain Reaction ◽  
The Czech Republic ◽  
Untreated Wastewater ◽  
Preliminary Study ◽  
Polymerase Chain

The virus SARS-CoV-2, which has caused the recent COVID-19 pandemic, may be present in the stools of COVID-19 patients. Therefore, we aimed to detect SARS-CoV-2 in wastewater for surveillance of SARS-CoV-2 in the population. Samples of untreated wastewater were collected from 33 wastewater treatment plants (WWTPs) of different sizes within the Czech Republic. SARS-CoV-2 RNA was concentrated from wastewater and viral RNA was determined using real-time reverse transcription polymerase chain reaction (RT-qPCR). SARS-CoV-2 RNA was detected in 11.6% of samples and more than 27.3% of WWTPs; in some of them, SARS-CoV-2 was detected repeatedly. Our preliminary results indicate that an epidemiology approach that focuses on the determination of SARS-CoV-2 in wastewater could be suitable for SARS-CoV-2 surveillance in the population.

scholarly journals Detection of pathogenic Yersinia enterocolitica serotype O:3 by biochemical, serological, and PCR methods

2008 ◽  
Vol 25 (No. 4) ◽  
pp. 214-220 ◽  
Author(s):  
J. Simonová ◽  
M. Vázlerová ◽  
I. Steinhauserová
Keyword(s):  
Czech Republic ◽  
Yersinia Enterocolitica ◽  
Virulence Genes ◽  
Optimum Time ◽  
The Czech Republic ◽  
Specific Identification ◽  
Time Consumption ◽  
Serotype O

In this study, the pathogenic <i>Y. enterocolitica</i> of serotype O:3 was monitored. The serotype is widely spread in Europe and has been linked to human yersiniosis. For the detection of pathogenic strains were used biochemical and serological methods as well as PCR methods based on the identification of virulence genes (<i>ail</i>, <i>rfbC</i>, <i>ystA</i>, <i>yadA</i>, <i>virF</i>). The occurrence of <i>Y. enterocolitica</i> O:3 strains was monitored in slaughter animals from a number of farms in the Czech Republic. A total of 3748 samples were collected coming from pigs (1388), cattle (633), poultry (902), and slaughter facilities (825). Fifty-two <i>Y. enterocolitica</i> O:3 isolates were identified by biochemical and serologic methods, and 53 <i>Y. enterocolitica</i> O:3 isolates were identified by PCR methods (46 isolates from pigs, 2 isolates from poultry, 3 isolates from cattle, and 2 isolates from a poultry slaughtering facility). All isolates of <i>Y. enterocolitica</i> O:3 carried genes <i>ail</i> and <i>rfbC</i>, 83% isolates carried gene <i>ystA</i>, 79% isolates carried gene <i>yadA</i> and 49% isolates carried gene <i>virF</i>. The use of PCR methods based on the identification of <i>ail</i> and <i>rfbC</i> genes provides for a sufficiently specific identification of pathogenic <i>Y. enterocolitica</i> O:3 strains with optimum time consumption compared to biochemical and serological methods. It is not recommendable to use other PCR methods (detection of the <i>ystA, <i>yadA</i>, and <i>virF</i> genes) for the detection of pathogenic <i>Y. enterocolitica</i> strains because those methods are not very specific for the determination of pathogenicity.

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