scholarly journals Effects of ridge and furrow film mulching on soil environment and yield under potato continuous cropping system

2019 ◽  
Vol 65 (No. 11) ◽  
pp. 523-529 ◽  
Author(s):  
Yanling Fan ◽  
Weina Zhang ◽  
Yichen Kang ◽  
Zhangping Zhao ◽  
Kai Yao ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Solanum Tuberosum L ◽  
Soil Microbial Communities ◽  
Cropping System ◽  
Continuous Cropping ◽  
Soil Environment ◽  
Soil Microbial ◽  
Film Mulching ◽  
Continuous Cropping System ◽  
Experimental Treatments

The effect of ridge and furrow film mulching (RFFM) on soil microbial communities, physicochemical property, enzymatic activity, and tuber yield were studied under the potato (Solanum tuberosum L.) continuous cropping fields managed for one (CY-1), two (CY-2) and four years (CY-4). Experimental treatments included a conventional flat plot without film mulching (FP) and five RFFM treatments: (i) a flat plot with film mulching (T1); (ii) on-ridge planting with full film mulching (T2); (iii) on-furrow planting with full film mulching (T3); (iv) on-ridge planting with half film mulching (T4); and (v) on-furrow planting with half film mulching (T5). Compared with FP, RFFM increased microbial communities, soil electrical conductivity, and enzymatic activities, and decreased pH values to improve the soil environment. Microbial communities of T2 and T5 were relatively higher, while soil urease and alkaline phosphatase activities of T2 and T4 were higher in all the continuous cropping years. In addition, the tuber yields of T2 were also increased by 75.0, 46.4 and 97.3% than FP, respectively. Thus, on-ridge planting with full film mulching (T2) is recommended as an adaptive management strategy for potato cultivation in the semi-arid areas where continuous cropping is necessary.

scholarly journals Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities

2009 ◽  
Vol 55 (No. 10) ◽  
pp. 413-423 ◽  
Author(s):  
V. Valášková ◽  
P. Baldrian
Keyword(s):  
Microbial Communities ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Soil Microbial Communities ◽  
Soil Microbial ◽  
Genes Encoding ◽  
Gradient Gel Electrophoresis ◽  
Temperature Gradient Gel Electrophoresis ◽  
Experimental Treatments ◽  
Multiple Samples

In soil microbial ecology, the effects of environmental factors and their gradients, temporal changes or the response to specific experimental treatments of microbial communities can only be effectively analyzed using methods that address the structural differences among whole communities. Fingerprinting methods are the most appropriate technique for this task when multiple samples must be analyzed. Among the methods currently used to compare microbial communities based on nucleic acid sequences, the techniques based on differences in the melting properties of double-stranded molecules, denaturing gradient gel electrophoresis (DGGE) or temperature gradient gel electrophoresis (TGGE), are the most widely used. Their main advantage is that they provide the possibility to further analyze whole sequences contained in fingerprints using molecular methods. In addition to the analysis of microbial communities based on DNA extracted from soils, DGGE/TGGE can also be used for the assessment of the active part of the community based on the analysis of RNA-derived sequences or for the analysis of sequences of functional genes encoding for proteins involved in important soil processes.

scholarly journals Continuous monocropping highly affect the composition and diversity of microbial communities in peanut (Arachis hypogaea L.)

2021 ◽  
Vol 49 (4) ◽  
pp. 12532
Author(s):  
Ali I. MALLANO ◽  
Xianli ZHAO ◽  
Yanling SUN ◽  
Guangpin JIANG ◽  
Huang CHAO
Keyword(s):  
Microbial Communities ◽  
Management Practices ◽  
Rhizosphere Soil ◽  
Diversity Index ◽  
Cropping Systems ◽  
Soil Microbial Communities ◽  
Pathogenic Fungi ◽  
Continuous Cropping ◽  
Bacterial Phyla ◽  
Soil Microbial

Continuous cropping systems are the leading cause of decreased soil biological environments in terms of unstable microbial population and diversity index. Nonetheless, their responses to consecutive peanut monocropping cycles have not been thoroughly investigated. In this study, the structure and abundance of microbial communities were characterized using pyrosequencing-based approach in peanut monocropping cycles for three consecutive years. The results showed that continuous peanut cultivation led to a substantial decrease in soil microbial abundance and diversity from initial cropping cycle (T1) to later cropping cycle (T3). Peanut rhizosphere soil had Actinobacteria, Protobacteria, and Gemmatimonadetes as the major bacterial phyla. Ascomycota, Basidiomycota were the major fungal phylum, while Crenarchaeota and Euryarchaeota were the most dominant phyla of archaea. Several bacterial, fungal and archaeal taxa were significantly changed in abundance under continuous peanut cultivation. Bacterial orders, Actinomycetales, Rhodospirillales and Sphingomonadales showed decreasing trends from T1>T2>T3. While, pathogenic fungi Phoma was increased and beneficial fungal taxa Glomeraceae decreased under continuous monocropping. Moreover, Archaeal order Nitrososphaerales observed less abundant in first two cycles (T1&T2), however, it increased in third cycle (T3), whereas, Thermoplasmata exhibit decreased trends throughout consecutive monocropping. Taken together, we have shown the taxonomic profiles of peanut rhizosphere communities that were affected by continuous peanut monocropping. The results obtained from this study pave ways towards a better understanding of the peanut rhizosphere soil microbial communities in response to continuous cropping cycles, which could be used as bioindicator to monitor soil quality, plant health and land management practices.

Alleviating Continuous Monocropping Obstacle in Melon: Biological Elimination of Phenolic Acid

2020 ◽  
Author(s):  
Huiqin Xie ◽  
Yongli Ku ◽  
Xiangna Yang ◽  
Le Cao ◽  
Xueli Mei ◽  
...  
Keyword(s):  
Microbial Community ◽  
Microbial Communities ◽  
Phenolic Acids ◽  
Fruit Quality ◽  
High Performance ◽  
Soil Microbial Communities ◽  
Illumina Miseq ◽  
Continuous Cropping ◽  
Soil Microbial

Abstract Background: Melon (Cucumis melo L.) is one of the most important fruit crops grown in China. However, the yield and quality of melon have significantly declined under continuous cropping. Phenolic acids are believed to be associated with the continuous monocropping obstacle (CMO) and can influence plant microbe interactions. Coumaric acid (CA) is one of the major phenolic acids found in melon root exudates. The objectives of this study were to estimate the elimination of CA by the soil bacterium K3 as well as its effects on mitigating melon CMO. CA degradation was investigated by monitoring the CA retained in the growth medium using high performance liquid chromatography (HPLC). The effects of CA and K3 on rhizosphere soil microbial communities were investigated by the spread plate method and Illumina MiSeq sequencing. Furthermore, the effects of CA and K3 on melon seedling growth were measured under potted conditions. The changes in soil enzymes and fruit quality under K3 amendment were examined in a greenhouse experiment. Result:The results suggest that the addition of CA had the same result as the CMO, such as deterioration of the microbial community and slower growth of melon plants. HPLC and microbial analysis showed that K3 had a pronounced ability to decompose CA and could improve the soil microbial community environment. Soil inoculation with K3 agent could significantly improve the fruit quality of melon.Conclusion: Our results show that the effects of K3 in the soil are reflected by changes in populations and diversity of soil microbes and suggest that deterioration of microbial communities in soil might be associated with the growth constraint of melon in continuous monoculture systems.

Soil microbial communities under film mulching and N fertilization in semiarid farmland

2019 ◽  
Vol 114 (2) ◽  
pp. 157-170 ◽  
Author(s):  
Shasha Luo ◽  
Shaojie Wang ◽  
Pengwei Yao ◽  
Dan Guo ◽  
Xiujun Li ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Soil Microbial Communities ◽  
N Fertilization ◽  
Soil Microbial ◽  
Film Mulching

scholarly journals Effects of Cropping Practice on Bupleurum Chinense Rhizosphere Microbial Community

2021 ◽  
Author(s):  
Li Liu ◽  
Hailu Cao ◽  
Yannan Geng ◽  
Ya Fan ◽  
Haiyang Feng ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Rhizosphere Soil ◽  
Soil Microbial Communities ◽  
Chemical Properties ◽  
Alpha Diversity ◽  
Soil Chemical Properties ◽  
Continuous Cropping ◽  
Factors Affecting ◽  
Soil Microbial ◽  
Cropping Practices

Abstract It is of great importance to understand the effects of cropping practices of Bupleurum chinense on the properties of rhizosphere soil. Therefore, the chemical properties of rhizosphere soil and the rhizosphere microbiome were assessed in the field trial with Bupleurum and three cropping practices (continuous monocropping, Bupleurum-corn intercropping and Bupleurum-corn rotation). The results showed cropping practices changed the chemical properties of the rhizosphere soil and composition, structure and diversity of the rhizosphere microbial communities. Continuous monocropping of Bupleurum chinense not only decreased soil pH and the contents of NO3--N and available K, but also decreased the alpha diversity of bacteria and beneficial microorganisms. However, Bupleurum-corn rotation improved soil chemical properties and reduced the abundance of harmful microorganisms. Soil chemical properties, especially the contents of NH4+-N, soil organic matter (SOM) and available K, were the key factors affecting the structure and composition of microbial communities in the rhizosphere soil. These findings could provide a new basis for overcoming problems associated with continuous cropping and promote development of B. chinense planting industry by improving soil microbial communities.

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