scholarly journals Differences between metabolic profiles of egg-type and meat-type hybrid hens

2011 ◽  
Vol 49 (No. 8) ◽  
pp. 323-328 ◽  
Author(s):  
P. Suchý ◽  
E. Straková ◽  
B. Jarka ◽  
J. Thiemel ◽  
V. Večerek
Keyword(s):  
Blood Plasma ◽  
Biochemical Parameters ◽  
Metabolic Profile ◽  
Egg Laying ◽  
Haematocrit Level ◽  
Production Type ◽  
Significant Difference ◽  
Biochemical Analyses ◽  
Meat Type ◽  
In Blood Plasma

The main objective of this study was to compare the metabolic profile (i.e. selected haematological and biochemical parameters in blood plasma) of production egg-type hybrid hens (E, MORAVIA BSL) with that of meat-type hybrid hens (M, COBB 500), and to evaluate dynamic changes occurring during the laying period from the 25th to 50th week of age. The study was prompted by both poultry farmers and veterinary practitioners since parameters defining the health of layers are absent. Selected parameters of the metabolic profile of layers were monitored regularly in 5-week intervals during egg laying. Samples of blood for haematological and biochemical examination were obtained by puncture of the vena basilica and stabilized by heparin. The study has revealed that monitored parameters of the metabolic profile significantly vary with factors such as production type, nutrition, and egg-laying intensity. Enhanced metabolism in egg-type hens was reflected in erythropoiesis which increased (in comparison with meat-type hens) highly significantly (P ≤ 0.01), particularly for erythrocyte count 2.36 T/l (2.14 T/l), haematocrit level 0.32 l/l (0.30 l/l), content of haemoglobin 94.48 g/l (84.18 g/l) and leukocyte count 17.06 G/l (13.22 G/l). Similarly, enhanced metabolism in egg-type hens also led to a highly significant increase (P ≤ 0.01) in the levels of biochemical parameters in blood plasma, particularly the total level of protein was 57.62 g/l (50.98) g/l), glucose 14.03 mmol/l (13.36 mmol/l), cholesterol 5.34 mmol/l (3.73 mmol/l) and calcium 6.52 mmol/l (5.52 mmol/l). ) In the case of the plasma level of phosphorus, no significant difference was found between egg-type hens (1.58 mmol/l) and meat-type hens (1.59 mmol/l). The reported results are based on the total of 180 haematological and biochemical analyses performed during the laying period. The results are crucial not only for future development of avian haematology and biochemistry, but also in respect to veterinary practice.    

Effect of Dietary Copper and Zinc Supplementation on Semen Quality of Murrah Bulls

2020 ◽  
Author(s):  
Abhradip Majumder ◽  
Manjula Thakur ◽  
Mukesh Bhakat ◽  
Manorama Saha ◽  
Tushar Kumar Mohanty ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Seminal Plasma ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Basal Diet ◽  
Sod Activity ◽  
Significant Difference ◽  
T1 And T2 ◽  
Cu And Zn ◽  
In Blood Plasma

The effect of dietary supplementation of Cu and Zn on semen quality parameters and certain bio-chemicals parameters were evaluated in Murrah bulls. Twelve mature Murrah bulls (4-6 years of age) were divided into three groups (n=4) T1, T2, and T3 based on semen volume and concentration and were fed as per ICAR standard (2013). However, the animals were supplemented with 0%, 25%, and 50% Cu and Zn above the basal diet in T1, T2 and T3 groups, respectively for 180 days. Quantitative and qualitative characteristics of semen, blood and seminal plasma antioxidant status, blood and semen minerals (Cu, Zn, Ca and Mn) were determined in experimental Murrah bulls. Semen ejaculate volume (mL) increased in T2 and T3 while sperm concentration (million/mL), intact acrosome (%), HOST reacted spermatozoa (%) increased in T3 group compared to T1 and T2 groups. No difference was observed in mass motility, pH, live spermatozoa (%) in semen sexual behaviour except dismounting time which was decreased in T3 than T1 and T2 (plessthan0.05). SOD activity in blood plasma and LPO activity decreased in seminal plasma in both T2 and T3 groups than T1, whereas catalase activity did not show any significant difference. Cu and Zn supplementation in T2 group improved plessthan0.05) Zn level in blood and seminal plasma and Cu level in blood plasma only, but not in seminal plasma. Therefore, it can be concluded that supplementation of Zn and Cu at 50% above the recommended levels of ICAR (2013) improved the qualitative and quantitative attributes of semen in Murrah bulls.

SEX HORMONES IN FISH. PART II. THE OESTROGENS OF SCYLIORHINUS CANICULUS

1963 ◽  
Vol 26 (4) ◽  
pp. 499-507 ◽  
Author(s):  
T. H. SIMPSON ◽  
R. S. WRIGHT ◽  
S. V. HUNT
Keyword(s):  
Blood Plasma ◽  
Sex Hormones ◽  
Ovarian Tissue ◽  
Constant Independent ◽  
L Protein ◽  
Significant Difference ◽  
Whole Ovaries ◽  
In Blood Plasma

SUMMARY Whole ovaries of the lesser spotted dogfish (Scyliorhinus caniculus) have been shown to contain oestradiol-17β and oestrone, each in a concentration of ca. 19 μg./kg., corresponding to approximately 0·8 μg./fish. The greater part (approximately 80%) of this material is localized within the eggs, the remainder being found in the ovarian tissue. The concentrations of oestradiol and oestrone in the eggs appear to be constant, independent of the size of egg, thus the amounts of these compounds in the egg increase as the egg matures. Free oestradiol-17β and oestrone have been found in blood plasma in respective concentrations of between 3 and 4 μg./l. and 0·5–1 μg./l.; protein-bound oestrone and oestradiol also appeared to be present, though in quantities too small for adequate characterization. No conjugated oestrogens could be detected. There is a significant difference between the ratios of oestradiol to oestrone in the eggs, ovarian tissue and blood plasma.

scholarly journals Attenuation by leptin of the effects of fasting on ovarian function in hens (Gallus domesticus)

Reproduction ◽  
2003 ◽  
pp. 739-751 ◽  
Author(s):  
HE Paczoska-Eliasiewicz ◽  
A Gertler ◽  
M Proszkowiec ◽  
J Proudman ◽  
A Hrabia ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Leptin Receptor ◽  
Ovarian Function ◽  
Laying Hens ◽  
Egg Laying ◽  
Leptin Receptors ◽  
Peripheral Effect ◽  
Ad Libitum ◽  
Induced Apoptosis ◽  
In Blood Plasma

Thirty-four-week-old laying hens received injections of recombinant chicken leptin to assess the role of leptin in avian ovarian function. In the first experiment, the hens (n=60) were divided into three groups: (i). fed ad libitum; (ii). fasted; and (iii). fasted + leptin. Hens were fasted for 5 days and those treated with leptin received 250 microg leptin kg-1 body weight twice a day, i.p. In the second experiment, the hens (n=72) were divided into four groups: (i). fed ad libitum; (ii). fasted; (iii). fasted + leptin given only during fasting (5 days); or (iv). fasted and leptin given during both fasting and 5 days of re-feeding (10 days). LH was measured in blood plasma, and progesterone and oestradiol were measured in blood plasma and the ovary by radioimmunoassay. Apoptosis was examined in the walls of the three largest yellow hierarchical follicles (F3-F1; F3 25-35 mm) by the TdT-mediated dUTP nick-end labelling method. Results showed that the injections of leptin during fasting: (i). delayed cessation of egg laying; (ii). attenuated regression of yellow hierarchical follicles; (iii). altered ovarian steroidogenesis; and (iv). abolished the fasting-induced apoptosis in the wall of F3-F1 follicles during the first 2 days of fasting and partially attenuated apoptosis after 5 days of fasting. Prolongation of leptin injections into the re-feeding period considerably delayed the restoration of the ovary. Expression of leptin receptor in laying hens was determined by RT-PCR. The highest expression of leptin receptor was observed in the hypothalamus. Lower receptor mRNA expression was found in the hypophysis, whereas the lowest expression was observed in the ovary. Within the ovary, a relatively high expression of leptin receptor was found in the stroma with cortical follicles <1 mm, the wall of white (1-8 mm) and small yellow follicles (>8-12 mm), and the granulosa layer of F3 follicles. The expression of leptin receptor in the granulosa layer of F2 and F1 follicles was barely detectable. This was in contrast to a much higher expression of leptin receptor maintained in the theca layer of F3-F1 follicles. The present results indicate that in chickens leptin might be involved in the adaptation to starvation due to attenuation of follicular apoptosis. The presence of leptin receptors in the ovary indicates the possibility of a peripheral effect of the hormone.

scholarly journals The effect of carnitine on hatching rate and metabolic profile of blood in breeding layers

2011 ◽  
Vol 49 (No. 12) ◽  
pp. 517-523 ◽  
Author(s):  
J. Thiemel ◽  
P. Jelínek
Keyword(s):  
Blood Plasma ◽  
Biochemical Parameters ◽  
Metabolic Profile ◽  
Practical Importance ◽  
Hatching Rate ◽  
Egg Hatching ◽  
New Knowledge ◽  
Positive Effect ◽  
Scientific Aspect ◽  
Biochemical Examination

We examined the effect of orally administered L-carnitine (at a dose of 30 mg per kg of feeding mixture) on egg hatching and some haematological and biochemical parameters of blood plasma in COBB 500 breeding layers of meat type. The experimental results confirmed a positive effect of L-carnitine, which increased the hatching rate significantly (P &le; 0.05) by 8.89% as compared to the control. This positive effect resulted from a highly significant (P &le; 0.01) decrease in the number of unfertilized eggs in experimental layers (4.44%), as compared to the control (12.2%). Haematological tests such as total counts of erythrocytes and leukocytes showed no differences between control and experimental layers. However, the average levels of haematocrit and haemoglobin in experimental layers were significantly lower (P &le; 0.01) (0.31 l/l and 85.67 g/l) in comparison with the control (0.33&nbsp;l/l and 89.92 g/l). Biochemical examination of the samples of blood plasma collected from experimental layers after the administration of L-carnitine revealed higher levels of glucose and magnesium and lower levels of total protein, cholesterol, AST, calcium and phosphorus, as compared to the control. The differences in the average levels of the monitored biochemical parameters between control and experimental layers were found highly significant (P &le; 0.01). The results provide new knowledge of the effect of L-carnitine on the metabolism of layers. These results are important not only from the scientific aspect, they are also of practical importance and can be used to formulate diets for breeding layers in order to enhance reproduction. &nbsp;

scholarly journals Assessment of changes in blood plasma biochemical indices at hypercholesterol diet with a high fat load

2021 ◽  
Vol 100 (6) ◽  
pp. 617-622
Author(s):  
Natalya F. Kushnerova ◽  
Yury A. Rakhmanin ◽  
Tatiana V. Momot ◽  
Rufina I. Mikhailova ◽  
Irina N. Ryzhova ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Biochemical Parameters ◽  
Fatty Acid Esters ◽  
Male Rats ◽  
High Density Lipoproteins ◽  
Cholesterol Diet ◽  
High Fat ◽  
Lipid Complex ◽  
The Impact ◽  
In Blood Plasma

Introduction. It was studied the lipid composition of the blood plasma of rats under the impact of a hyper cholesterol diet with a high fat load. It was carried out the prevention of disturbances in blood plasma biochemical parameters with a lipid complex from the tunic of the marine hydrobiont Halocynthia aurantium. Materials and methods. The experiment was carried outwith outbred male rats weighing 200 ± 3 g.The experimental model of a hyper cholesterol diet with a high fat load with the development of dyslipidemia was set up by feeding the animals with ahigh fat diet consisting of 2% cholesterol and 20% beef fat from the total diet. The animals were divided into the following groups of 10 rats each: group 1 - control (standard diet), group 2 - dyslipidemia (hypercholesterol diet with high fat load), group 3 - dyslipidemia + lipid complex from ascidia. Results. It was shown that the influence of the diet was accompanied by an increase in the amount of total lipids in the blood plasma of rats, cholesterol, low-density lipoproteins (LDL), as well as a decrease in total phospholipids and high-density lipoproteins (HDL), which is considered as an indicator of the formation of dyslipidemia. The contents of phospholipid lysofractions increased due to the activation of phospholipases. The amount of fatty acid esters and cholesterol esters decreased, which indicates the inhibition of esterification processes. The imbalance in the phospholipid spectrum of blood plasma occurred: the amount of metabolically active fractions required for the functioning of membrane-bound enzymes decreased. The addition of a lipid complex from the tunic of ascidian purple into the diet was accompanied by a pronounced prophylactic effect, which manifested itself in the normalization of the studied biochemical parameters. The lipid complex containing a wide range of “sea” phospholipids and polynonsaturated fatty acids of the n-3 type is an important basis for application as prophylactic in the conditions of a hypercholesterol diet with a high-fat load. Conclusion. Application of the lipidic complexes containing the “sea” lipids allocated from a tunic of the ascidian purple can be useful and perspective at a dislipidemiya and a hypercholesterolemia that will allow to carry out effective prevention of violations of metabolic reactions at influence of hyper high-calorie food.

scholarly journals LARGE INTESTINE NORMOBIOCENOSIS AND PROOXIDANT-ANTIOXIDANT BALANCE OF COLONOCYTES, BLOOD PLASMA IN EXPERIMENTAL DISBIOOSIS AND USAGE OF RIOFLORA IMMUNO NEO PROBIOTIC

2018 ◽  
pp. 27-33
Author(s):  
A. V. Shevchenko ◽  
O. A. Medvedeva ◽  
A. Yu. Mukhina ◽  
V. A. Korolev ◽  
P. V. Kalutsky
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Blood Plasma ◽  
Large Intestine ◽  
Biochemical Parameters ◽  
Malonic Dialdehyde ◽  
Antioxidant Defense System ◽  
Colon Tissue ◽  
Corrective Effect ◽  
In Blood Plasma

Aim. Study the changes in the composition of animals large intestine microbiocenosis and molecular-biochemical parameters of blood plasma and colonocytes in experimental gentamicin dysbiosis, possibility of correction using probiotic. Materials and methods. Drug dysbiosis was simulated by administration of gentamicin intraperitoneally and with correction aim injected probiotic. Quantitative and qualitative study of mucous microflora of the mice large intestine was performed by bacteriological method. The state of lipid peroxidation system was judged about by content of acylhydroperoxide and malonic dialdehyde, antioxidant protection system - by catalase and superoxide dismutase. Results. As a result of the study, a change in the composition of the intestinal microflora, a decrease in the enzymatic activity of antioxidant defense system in blood plasma and colonocytes, an increase in the content of lipid peroxidation products in blood plasma and colonocytes were recorded. The corrective effect of RioFlora Immuno Neo probiotic regarding the restoration of the intestine normobiocenosis and the molecular-biochemical parameters of animal colonocytes was noted. Conclusion. The use of the probiotic led to the restoration of microbial equilibrium in the intestinal microbiocenosis, and also had a positive effect on the superoxide dismutase activity of colonocytes, stabilization of the malonic dialdehyde content in the colon tissue.

scholarly journals Effect of Diet Supplemented with Dennettia tripetala Fruits on Serum Antioxidants and Biochemical Parameters of Albino Rats

2020 ◽  
Vol 4 (1) ◽  
pp. 16-21
Author(s):  
Adebiyi H. Yetunde ◽  
Dauda Muhammed ◽  
Babatunde O. Richard ◽  
Odey O. Bernard ◽  
Aderemi C. Omolaiye ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Albino Rats ◽  
Hypolipidemic Effect ◽  
Formulated Diet ◽  
Significant Difference ◽  
Dennettia Tripetala ◽  
Group 2 ◽  
Biochemical Analyses

AbstractThis study was carried out to determine the effect of dietary supplementation of Dennettia tripetala on some biochemical parameters of rats. Twenty-five rats were used and were divided in to five groups of 5 rats. Group 1 served as control while group 2-5 were fed dietary inclusion of Dennettia tripetala fruits for 28 days. After the feeding trials, the rats were sacrificed and blood was collected for biochemical analyses. The serum concentration triglyceride and glucose were significantly lowered (p<0.05) while total protein, catalase and high-density lipoprotein were significantly higher (p<0.05) in rats fed with the formulated diet. There was no significant difference (p<0.05) in the serum level of superoxide dismutase, albumin, cholesterol and low-density lipoprotein of rats fed with the formulated diet. In conclusion, the study has shown that dietary inclusion of Dennettia tripetala fruits has hypoglycaemic, antioxidant and hypolipidemic effect. Therefore, it may help in the prevention of coronary heart disease, diabetes mellitus and cancer.

scholarly journals Effects of Energy Drink in Combination with Two Different Brands of Alcohol on Some Biochemical Parameters of Male Albino Rats

2020 ◽  
Vol 5 (9) ◽  
pp. 707-710
Author(s):  
Okuda, A. Frank ◽  
Ehiwario, N. Julius ◽  
Oshilim, O. Anthony
Keyword(s):  
Biochemical Parameters ◽  
Energy Drink ◽  
Control Group ◽  
Albino Rats ◽  
Treatment Groups ◽  
Group 4 ◽  
Significant Difference ◽  
Group 2 ◽  
Biochemical Analyses ◽  
Group 1

This study was carried out to ascertain the effect of energy drink and mixture of energy drink with different brands of alcohol on some biochemical parameters. Sixteen (16) male albino rats weighing 120- 160g were divided into four (4) groups. The groups were divided thus: Group 1: Control- feed and water ad libitum, Group 2: 3.75ml/kg Bullet Energy drink only, Group 3: 3.75ml/kg Bullet energy drink + 1ml/kg of 41% alcohol dry gin and Group 4: 3.75ml/kg Bullet energy drink + 1ml/kg of 5.1% alcoholic beer. The experiment lasted for 28 days after which the animals were fasted for 12 hours before they were sacrificed and their blood collected for biochemical analyses. There was a significant increase in body weight of animals in group 2-4 and also the glucose concentration was elevated. The result presented in table 2 showed that there is a statistically significant difference (p<0.05) in the level of ALT and ALP in treatment groups when compared to the control group (1). Also, elevated level of cholesterol and triglycerides were also observed. From this data, we may conclude that there are high risk of constantly taking energy drink alone or in combination with alcohol.

scholarly journals Influence of vitamin-mineral supplement on protein metabolism in rabbits’ organisms

2021 ◽  
Vol 4 (1) ◽  
pp. 3-6
Author(s):  
M. Fedorchenko
Keyword(s):  
Blood Plasma ◽  
Protein Content ◽  
Biochemical Parameters ◽  
The Body ◽  
Control Group ◽  
Total Protein Content ◽  
Mineral Supplement ◽  
Significant Difference ◽  
Positive Effect ◽  
Alt Activity

The purpose of the study is to study the influence of different vitamin-mineral supplement “Tekro” doses on biochemical parameters in blood plasma and liver of rabbits of New Zealand breed. The research methodology is based on research according to generally accepted research methods. Biochemical parameters were studied in rabbits’ organisms at 45, 60, 75 and 90 days of rearing with feeding of different doses of vitamin-mineral supplement “Tekro”, namely 3 %, 3.5 %, 4 %. The academic novelty is that nowadays the problem of the effect of different vitamin-mineral supplement “Tekro” doses on biochemical parameters in the blood and liver of rabbits has not been studied. Analyzing the indicators of the total protein content in the blood plasma of rabbits of the experimental groups and the control one for 90 days, it was noted that there was no significant difference in the indicators. In the liver of rabbits of the age from 45 to 90 days there was a tendency to increase the protein content due to the effects on the body of various doses of vitamin and mineral supplement “Tekro”. It has been established the positive effect of vitamin-mineral supplement “Tekro” on ALT activity of rabbits in experimental groups. At the age of 75 days, significantly higher ALT activity was recorded in rabbits of the 3rd experimental group by 35.8 % compared to animals in the control group.

scholarly journals The status of antioxidative system in pregnant women infected with human papilloma virus

2010 ◽  
Vol 17 (3-4) ◽  
pp. 83-93
Author(s):  
Birutė SURINĖNAITĖ ◽  
Gintautas DOMŽA ◽  
Janina DIDŽIAPETRIENĖ ◽  
Gražina PRASMICKIENĖ ◽  
Audronė JAKAITIENĖ
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Blood Plasma ◽  
Pregnant Women ◽  
First Trimester ◽  
Hpv Infection ◽  
Antioxidative System ◽  
Papilloma Virus ◽  
Significant Difference ◽  
In Blood Plasma

Background. The concentration of reduced (GSH), oxidized (GSSG) and total (GSH + GSSG) glutathione, the level of malondialdehyde (MDA), and the general antioxidant activity (GAA) was determined in blood plasma and cervicovaginal washing fluid of pregnant women infected and non-infected with human papilloma virus (HPV). Materials and methods. The population of 213 pregnant women visiting one of the Centers of the Central Outpatient Clinic of Vilnius (Lithuania) in 2008–2010 was included in the study. These women were examined for HPV infection and its type. The tests were performed in the first and third trimesters of pregnancy in blood plasma and cervicovaginal washing fluid. The MDA level was tested by the thiobarbituric acid assay. The amount of glutathione forms was determined using a recycling system with 5,5’-dithiobis 2-nitrobenzoic acid and glutathione reductase. General antioxidant activity (GAA) was measured by Tween 80 oxidation in a specific test. All parameters were analyzed spectrophotometrically. Results. The concentration of all glutathione forms and GAA reduced during pregnancy, while the MDA level was enhanced in the blood plasma of all women included in the analysis. Only two of these parameters changed in the cervicovaginal washing fluid. No significant change of GAA was determined comparing groups of women while considering HPV infection. The GSH / GSSG ratio significantly increased in HPV-negative and decreased in HPV-positive women. While comparing HPV-positive and HPV-negative women of similar age, no significant difference of any parameter was found in cervicovaginal washing fluid and in blood plasma in the third trimester. In the first trimester the GSSG level was significantly lower, the GSH level was higher, and the GSH / GSSG ratio was twice higher in HPV-infected women. All pregnant women were compared with non-pregnant women to confirm the influence of pregnancy on the MDA level. In the first trimester, this parameter for pregnant women was close to that of non-pregnant women, while with the developing pregnancy the MDA level increased 1.6 times. No significant difference in the parameter was determined for pregnant women with HPV infection. Conclusions. A decrease of GSH concentration and an increase of MDA level in blood plasma with the development of pregnancy confirmed the presence of a general oxidative stress. The lower levels of GSH and GSH + GSSG in the cervicovaginal washing fluid, if to compare the two trimesters of pregnancy, can be considered as markers of a local oxidative stress. HPV infection depressed the antioxidative system in general and did not affect it at the local level. Systemic oxidative stress, rather than HPV infection, influenced the lipid peroxidation process during pregnancy. The level of MDA is recommended to be tested also during a normal pregnancy, although this parameter should not be considered as an additional biomarker of cervical carcinoma risk. Changes of the antioxidative system variables could induce a deep oxidative imbalance during a pathological pregnancy, and oxidative stress might cause a persistent HPV infection, suggesting the importance of the additional screening of HPV-infected women after delivery. Keywords: HPV infection, pregnancy, antioxidative system

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