scholarly journals Effect of microbial oil, monensin and fumarate on rumen fermentation in artificial rumen

2011 ◽  
Vol 50 (No. 10) ◽  
pp. 467-472 ◽  
Author(s):  
D. Jalč ◽  
M. Čertík
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Rumen Fermentation ◽  
Microbial Oil ◽  
Gamma Linolenic Acid ◽  
Microbial Synthesis ◽  
Fermentation Vessel ◽  
The Relationship ◽  
Stabilization Period

The objective of this study was to investigate the effect of microbial oil on rumen fermentation of a diet composed of 60% hay and 40% barley in an artificial rumen (Rusitec). Microbial oil (MO) was produced by the fungus Thamnidium elegans. This fungus grew on the wheat bran/spent malt grains (3:1) mixture. The fatty acid composition of microbial oil was as follows: 0.7% C<sub>14:0</sub>, 15.4% C<sub>16:0</sub>, 10.1% C<sub>18:0</sub>, 50.9% C<sub>18:1</sub>, 13.9% C<sub>18:2</sub> and 8.4% C<sub>18:3</sub> (GLA, &gamma;-linolenic acid). The effect of monensin MON (66 ppm) and fumarate FUM (6.25 mmol) with and without MO supplementation was also studied. The experiment in Rusitec lasted 11 days. After a stabilization period (5 days), MO was added to fermentation vessel V<sub>2</sub> (6 days), MON to fermentation vessel V<sub>3</sub> (6 days) and FUM to fermentation vessel V<sub>4 </sub>(6 days). MO was also added to V<sub>3</sub> and V<sub>4</sub> on the last day together with MON (V<sub>3</sub>) and FUM (V<sub>4</sub>). The fermentation vessel V<sub>1 </sub>served as control (without additives). The results showed that MO reduced (P &lt; 0.05) mol% acetate and increased (P &lt; 0.05) mol% propionate and n-butyrate. Methane production (mmol/day) was reduced numerically (NS). The efficiency of microbial synthesis (EMS) was also reduced numerically and nitrogen incorporated by the microflora (N<sub>M</sub>) was reduced significantly in MO supplementation. There were no differences in the rumen fermentation when MO was applied together with MON and FUM compared to the vessel where only MO was applied. No additive effect was observed in the relationship MO-ionophore or MO-FUM. Monensin and fumarate applied separately showed their typical effects on rumen fermentation in vitro. &nbsp;

scholarly journals Effect of microbial oil, evening primrose oil and borage oil on rumen fermentation in vitro

2012 ◽  
Vol 50 (No. 11) ◽  
pp. 480-486 ◽  
Author(s):  
D. Jalc ◽  
A. Potkanski ◽  
M. Szumacher-Strabel ◽  
A. Cieslak ◽  
M. Certik
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Content ◽  
Rumen Fermentation ◽  
Borage Oil ◽  
Microbial Oil ◽  
Evening Primrose Oil ◽  
Evening Primrose ◽  
Fermentation Vessel ◽  
Chain Fatty Acids

The objective of this study was to examine the effects of microbial oil, evening primrose oil and borage oil on rumen fermentation of a diet consisting of 80% of hay and 20% of barley in an artificial rumen (Rusitec). All three oils contained gamma-linolenic acid (GLA), microbial oil &ndash; 8.4%, evening primrose oil &ndash; 9.2% and borage oil &ndash; 23.7% out of the total fatty acid content. The experiment in Rusitec lasted 11 days. After a stabilization period (5 days), microbial oil (5% wt/wt) was added into fermentation vessel V<sub>2</sub>, evening primrose oil (5% wt/wt) into V<sub>3</sub> and borage oil (5%wt/wt) into V<sub>4</sub> (6 days). Fermentation vessel V<sub>1</sub> served as a control (without oils). The results showed that the oils did not affect any of the basal parameters of rumen fermentation (pH, NH<sub>3</sub>-N, degradation of dry matter, organic matter, neutral detergent fibre, acid detergent fibre). Methane production (mmol/day) was reduced numerically by the oils; microbial oil, evening primrose oil and borage oil decreased CH<sub>4</sub> production about 11.32%, 11.45% and 2.04%, respectively. The supplementation of the oils to the total mixed ration (TMR) significantly decreased percentage proportions of short-chain fatty acids (SCFA, about 0.1&ndash;0.3%), medium-chain fatty acids (MCFA, about 8%) and increased long-chain fatty acids (LCFA, about 8%) in the effluent. Stearic acid C<sub>18:0</sub> was the major FA in the effluent and was significantly reduced in oil supplemented diets. The percentage proportion of trans C<sub>18:1</sub> isomers significantly increased (1.7&ndash;2 times) in all oil supplemented diets. The main intermediates &ndash; cis 9, trans 11 C<sub>18:2</sub> (CLA) and trans 11 C<sub>18:1 </sub>(TVA) also increased after oil supplementation of the diet. TVA concentration with microbial oil, evening primrose oil and borage oil supplementation was 3.17%, 8.19% and 9.3% in comparison with the control (1.38%). CLA concentration significantly increased 2.3, 1.2, and 2.1 times after microbial oil, evening primrose oil and borage oil supplementation in Rusitec. Finally, the oil supplementation caused incomplete biohydrogenation of unsaturated FA and it was characterized by an increase in TVA concentration and TVA to C<sub>18:0</sub> ratio in oil supplemented diets.

scholarly journals Effect of a Rumen-Protected Microencapsulated Supplement from Linseed Oil on the Growth Performance, Meat Quality, and Fatty Acid Composition in Korean Native Steers

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1253
Author(s):  
Chae-Hyung Sun ◽  
Jae-Sung Lee ◽  
Jalil Ghassemi Nejad ◽  
Won-Seob Kim ◽  
Hong-Gu Lee
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Growth Performance ◽  
Meat Quality ◽  
Acid Composition ◽  
Linseed Oil ◽  
Control Group ◽  
Ruminal Fluid

We evaluated the effects of a rumen-protected microencapsulated supplement from linseed oil (MO) on ruminal fluid, growth performance, meat quality, and fatty acid composition in Korean native steers. In an in vitro experiment, ruminal fluid was taken from two fistulated Holstein dairy cows. Different levels of MO (0%, 1%, 2%, 3%, and 4%) were added to the diet. In an in vivo experiment, eight steers (average body weight = 597.1 ± 50.26 kg; average age = 23.8 ± 0.12 months) were assigned to two dietary groups, no MO (control) and MO (3% MO supplementation on a DM basis), for 186 days. The in vitro study revealed that 3% MO is an optimal dose, as there were decreases in the neutral detergent fiber and acid detergent fiber digestibility at 48 h (p < 0.05). The in vivo study showed increases in the feed efficiency and average daily gain in the 3% MO group compared to the control group on days 1 to 90 (p < 0.05). Regarding meat quality, the shear force produced by the longissimus thoracis muscle in steers from the 3% MO group was lower than that produced by the control group (p < 0.05). Interestingly, in terms of the fatty acid profile, higher concentrations of C22:6n3 were demonstrated in the subcutaneous fat and higher concentrations of C18:3n3, C20:3n3, and C20:5n3 were found in the intramuscular fat from steers fed with 3% MO (p < 0.05). Our results indicate that supplementation with 3% MO supplements improves the growth performance and meat quality modulated by the omega-3 fatty acid content of meat in Korean native steers.

scholarly journals Fatty acid composition of vegetable oil blend and in vitro effects of pharmacotherapeutical skin care applications

2019 ◽  
Vol 52 (2) ◽  
Author(s):  
M. Guidoni ◽  
M.M. de Christo Scherer ◽  
M.M. Figueira ◽  
E.F.P. Schmitt ◽  
L.C. de Almeida ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Vegetable Oil ◽  
Skin Care ◽  
In Vitro Effects ◽  
Oil Blend

scholarly journals The role of lipid components of the diet in the regulation of the fatty acid composition of the rat liver endoplasmic reticulum and lipid peroxidation

1978 ◽  
Vol 174 (2) ◽  
pp. 585-593 ◽  
Author(s):  
Catherine T. Hammer ◽  
Eric D. Wills
Keyword(s):  
Lipid Peroxidation ◽  
Fatty Acids ◽  
Endoplasmic Reticulum ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Polyunsaturated Fatty Acids ◽  
Acid Composition ◽  
Corn Oil ◽  
Lipid Peroxide

The fatty acid compositions of the lipids and the lipid peroxide concentrations and rates of lipid peroxidation were determined in suspensions of liver endoplasmic reticulum isolated from rats fed on synthetic diets in which the fatty acid composition had been varied but the remaining constituents (protein, carbohydrate, vitamins and minerals) kept constant. Stock diet and synthetic diets containing no fat, 10% corn oil, herring oil, coconut oil or lard were used. The fatty acid composition of the liver endoplasmic reticulum lipid was markedly dependent on the fatty acid composition of the dietary lipid. Feeding a herring-oil diet caused incorporation of 8.7% eicosapentaenoic acid (C20:5) and 17% docosahexaenoic acid (C22:6), but only 5.1% linoleic acid (C18:2) and 6.4% arachidonic acid (C20:4), feeding a corn-oil diet caused incorporation of 25.1% C18:2, 17.8% C20:4 and 2.5% C22:6 fatty acids, and feeding a lard diet caused incorporation of 10.3% C18:2, 13.5% C20:4 and 4.3% C22:6 fatty acids into the liver endoplasmic-reticulum lipids. Phenobarbitone injection (100mg/kg) decreased the incorporation of C20:4 and C22:6 fatty acids into the liver endoplasmic reticulum of rats fed on a lard, corn-oil or herring-oil diet. Microsomal lipid peroxide concentrations and rates of peroxidation in the presence of ascorbate depended on the nature and quantity of the polyunsaturated fatty acids in the diet. The lipid peroxide content was 1.82±0.30nmol of malonaldehyde/mg of protein and the rate of peroxidation was 0.60±0.08nmol of malonaldehyde/min per mg of protein after feeding a fat-free diet, and the values were increased to 20.80nmol of malonaldehyde/mg of protein and 3.73nmol of malonaldehyde/min per mg of protein after feeding a 10% herring-oil diet in which polyunsaturated fatty acids formed 24% of the total fatty acids. Addition of α-tocopherol to the diets (120mg/kg of diet) caused a very large decrease in the lipid peroxide concentration and rate of lipid peroxidation in the endoplasmic reticulum, but addition of the synthetic anti-oxidant 2,6-di-t-butyl-4-methylphenol to the diet (100mg/kg of diet) was ineffective. Treatment of the animals with phenobarbitone (1mg/ml of drinking water) caused a sharp fall in the rate of lipid peroxidation. It is concluded that the polyunsaturated fatty acid composition of the diet regulates the fatty acid composition of the liver endoplasmic reticulum, and this in turn is an important factor controlling the rate and extent of lipid peroxidation in vitro and possibly in vivo.

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