scholarly journals Activities of amine oxidase, peroxidase and catalase in seedlings of Pisum sativum L. under different light conditions

2011 ◽  
Vol 49 (No. 4) ◽  
pp. 151-157 ◽  
Author(s):  
L. Luhová ◽  
A. Lebeda ◽  
D. Hedererová ◽  
P. Peč
Keyword(s):  
Pisum Sativum ◽  
Enzyme Activities ◽  
Oxidase Activity ◽  
Amine Oxidase ◽  
Field Pea ◽  
Light Conditions ◽  
Green Plants ◽  
Pisum Sativum L ◽  
Etiolated Plants ◽  
Amine Oxidase Activity

The activities of amine oxidase, peroxidase and catalase were studied in 12 cultivars of field pea (Pisum sativum L.) and one accession of wild pea (Pisum sativum subsp. transcaucasicum). The influence of different light conditions on the enzyme activities was studied in extracts of 8-d-old seedlings. Substantially higher amine oxidase activity was detected in etiolated pea seedlings than in plants growing under controlled light conditions (12h photoperiod). Higher peroxidase and catalase activities indicate more intensive production of toxic hydrogen peroxide evolved by reactions of different type in green plants in comparison with etiolated ones. Significantly lower activity of peroxidases in etiolated plants could be related to a lower degree of lignification. Marked differences in enzyme activities between etiolated field pea and P. sativum subsp. transcaucasicum were observed for all studied enzymes. A very interesting result was the exceptionally low activity of amine oxidase in etiolated plants that was hardly detectable in green plants of Malton cultivar. Two bands with amine oxidase activity were found by the method of native PAGE in extracts of 8-d-old plants. A different relationship of these isoenzymes was detected in field pea and wild pea. Two isoenzymes were present in pea shoots but only one isoenzyme was detected in pea roots. Amine oxidase isoenzymes were studied in the roots and shoots of cv. Smaragd for three weeks. The profile of isoenzymes was opposite in 8- and 36-d-old stems of pea.

Mitochondrial Oxidation of p-N, N-Dimethylamino-β-Phenethylamine (DAPA)

1975 ◽  
Vol 33 ◽  
pp. 458-459
Author(s):  
W. Allen Shannon ◽  
Hannah L. Wasserkrug ◽  
andArnold M. Seligman
Keyword(s):  
Guinea Pig ◽  
Oxidase Activity ◽  
Amine Oxidase ◽  
Histochemical Demonstration ◽  
Guinea Pig Heart ◽  
Pig Kidney ◽  
Cytoplasmic Vacuoles ◽  
Liver And Kidney ◽  
Mitochondrial Oxidation ◽  
Amine Oxidase Activity

The synthesis of a new substrate, p-N,N-dimethylamino-β-phenethylamine (DAPA)3 (Fig. 1) (1,2), and the testing of it as a possible substrate for tissue amine oxidase activity have resulted in the ultracytochemical localization of enzyme oxidase activity referred to as DAPA oxidase (DAPAO). DAPA was designed with the goal of providing an amine that would yield on oxidation a stronger reducing aldehyde than does tryptamine in the histochemical demonstration of monoamine oxidase (MAO) with tetrazolium salts.Ultracytochemical preparations of guinea pig heart, liver and kidney and rat heart and liver were studied. Guinea pig kidney, known to exhibit high levels of MAO, appeared the most reactive of the tissues studied. DAPAO reaction product appears primarily in mitochondrial outer compartments and cristae (Figs. 2-4). Reaction product is also localized in endoplasmic reticulum, cytoplasmic vacuoles and nuclear envelopes (Figs. 2 and 3) and in the sarcoplasmic reticulum of heart.

Mitigation Effects of 24-Epibrassinolide and Thiourea in Field Pea (Pisum sativum L.) under Drought Stress

2018 ◽  
Vol 34 (2) ◽  
pp. 229-235 ◽  
Author(s):  
Prachi Garg ◽  
◽  
A. Hemantaranjan ◽  
Jyostnarani Pradhan ◽  
◽  
...  
Keyword(s):  
Drought Stress ◽  
Pisum Sativum ◽  
Field Pea ◽  
Pisum Sativum L

DS Admiral field pea

2002 ◽  
Vol 82 (4) ◽  
pp. 751-752 ◽  
Author(s):  
L. Andersen ◽  
T. Warkentin ◽  
O. Philipp ◽  
A. Xue ◽  
A. Sloan
Keyword(s):  
Powdery Mildew ◽  
Pisum Sativum ◽  
Powdery Mildew Resistance ◽  
Field Pea ◽  
Western Canada ◽  
Mildew Resistance ◽  
Pisum Sativum L ◽  
Cultivar Description ◽  
Good Yielding ◽  
Growing Region

DS Admiral, a yellow cotyledon field pea (Pisum sativum L.) cultivar, was released in 2000 by Agriprogress Inc., Morden, Manitoba. DS Admiral has a semileafless leaf type, powdery mildew resistance, medium sized, round seeds, and good yielding ability. DS Admiral is adapted to the field-pea-growing region of western Canada. Key words: Field pea, Pisum sativum L., cultivar description, powdery mildew resistance

Acrolein produced from polyamines as one of the uraemic toxins

2003 ◽  
Vol 31 (2) ◽  
pp. 371-374 ◽  
Author(s):  
K. Sakata ◽  
K. Kashiwagi ◽  
S. Sharmin ◽  
S. Ueda ◽  
K. Igarashi
Keyword(s):  
Renal Failure ◽  
Chronic Renal Failure ◽  
Oxidase Activity ◽  
Culture Medium ◽  
Cellular Proliferation ◽  
Amine Oxidase ◽  
Early Stage ◽  
Chronic Glomerulonephritis ◽  
Toxic Compound ◽  
Amine Oxidase Activity

It is well known that the addition of spermine or spermidine to culture medium containing ruminant serum inhibits cellular proliferation. This effect is caused by the products of oxidation of polyamines that are generated by serum amine oxidase. Among the products, we found that acrolein is a major toxic compound produced from spermine and spermidine by amine oxidase. We then analysed the level of polyamines (putrescine, spermidine and spermine) and amine oxidase activity in plasma of patients with chronic renal failure. It was found that the levels of putrescine and the amine oxidase activity were increased, whereas spermidine and spermine were decreased in plasma of patients with chronic renal failure. The levels of free and protein-conjugated acrolein were also increased in plasma of patients with chronic renal failure. An increase in putrescine, amine oxidase and acrolein in plasma was observed in all cases such as diabetic nephropathy, chronic glomerulonephritis and nephrosclerosis. These results suggest that acrolein is produced during the early stage of nephritis through kidney damage and also during uraemia through accumulation of polyamines in blood due to the decrease in their excretion into urine.

BELLEVUE FIELD PEA

1987 ◽  
Vol 67 (3) ◽  
pp. 805-806
Author(s):  
A. E. SLINKARD ◽  
R. A. A. MORRALL
Keyword(s):  
Pisum Sativum ◽  
Seed Weight ◽  
Field Pea ◽  
Pisum Sativum L ◽  
Cultivar Description

Bellevue field pea (Pisum sativum L.) was licensed in 1986. It is about 15% higher yielding than the check cultivars Century and Trapper. Bellevue has smooth seeds with yellow cotyledons. It has the le gene for shortened internodes and is about 6 d later maturing than Century and Trapper. Seed weight is about 179 g/1000 seeds, intermediate between Century and Trapper.Key words: Cultivar description, pea (field), Pisum sativum L.

Proximate and mineral composition of field peas

1997 ◽  
Vol 77 (1) ◽  
pp. 101-103 ◽  
Author(s):  
T. D. Warkentin ◽  
A. G. Sloan ◽  
S. T. Ali-Khan
Keyword(s):  
Pisum Sativum ◽  
Key Words ◽  
Mineral Composition ◽  
Seed Yield ◽  
Total Protein ◽  
Field Pea ◽  
Pisum Sativum L ◽  
Field Peas ◽  
Pea Seeds ◽  
Proximate And Mineral Composition

Field pea seeds from 10 cultivars grown at two locations in Manitoba in 1986 and 1987 were analyzed for proximate and mineral profiles. Cultivars differed significantly in their level of total protein, crude fat, ADF, and all minerals tested. However, differences were not extremely large and were comparable to European reports. Location-year also had a significant effect on the levels of total protein, ADF, and all minerals tested. In most cases, the warmest location-year produced relatively higher levels of minerals, ash, and total protein, and lower seed yield than the coolest location-year. Key words: Field pea, Pisum sativum L., mineral

Seasonal Incidence of Pulse Aphid (Aphis craccivora Koch.) and its Natural Enemies on Field Pea (Pisum sativum L.) in Relation to Some Abiotic Factors in Alluvial Zone of West Bengal

2021 ◽  
Author(s):  
S. Pal ◽  
S. Samanta ◽  
A. Banerjee
Keyword(s):  
Relative Humidity ◽  
Pisum Sativum ◽  
Natural Enemies ◽  
West Bengal ◽  
Insect Pests ◽  
Field Pea ◽  
Pest Population ◽  
Pisum Sativum L ◽  
Aphis Craccivora Koch ◽  
Weather Parameters

Background: Field pea, Pisum sativum L. is an important winter-season pulse crop. It is subjected to damage by both field and storage insect pests and approximately 10-15 per cent reduction in yield was reported due to the infestation of different insect pests. Among these, pulse aphid (Aphis craccivora Koch.) affects plant physiology directly by removal of nutrients or indirectly by dispersal of various viral diseases. The present investigation has been aimed to study the seasonal fluctuations of aphids and their natural enemies as well as their correlation. Another objective was to know the effect of various weather parameters on pulse aphids and their natural enemies which ultimately would be helpful to develop a forewarning model.Methods: The field experiment was conducted at the A-B Block Farm of Bidhan Chandra Krishi Viswavidyalaya, Nadia, West Bengal using two varieties of field pea (KPMR 935 and IFPD 122) during rabi seasons of two consecutive years of 2017-18 and 2018-19 following a Randomized Block Design with three replications. After recording the total population of both pests (nymphs and adults) and their natural enemies across the season, the mean population was worked out and used for correlation and regression studies along with the weather parameters. Result: Maximum aphid population was noticed during the peak pod formation stage of the crop irrespective of the varieties. The pest population was very strongly correlated with the incidence of coccinellid and ant population in both test varieties. Among the weather parameters, both maximum and minimum temperature and sunshine hour showed a positive correlation with the pest population and their natural enemies but relative humidity and rainfall showed a negative correlation. Regression studies indicated that temperature and relative humidity were the most influencing factors over the incidence of aphid in both the seasons.

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