scholarly journals Gender and age effects on the expression of genes related to lipid metabolism in broiler’s liver

2018 ◽  
Vol 63 (No. 3) ◽  
pp. 103-109
Author(s):  
A. de Souza Khatlab ◽  
A.P. Del Vesco ◽  
E. Gasparino ◽  
A.R. de Oliveira Neto
Keyword(s):  
Gene Expression ◽  
Lipid Metabolism ◽  
Weight Gain ◽  
Feed Intake ◽  
Broiler Chickens ◽  
Fatty Acid Synthase ◽  
Lipid Synthesis ◽  
Gender Effect ◽  
Expression Of Genes ◽  
Gender And Age

Two experiments were conducted to assess gender (Experiment 1) and age (Experiment 2) effects on the expression of genes related to lipid metabolism in broiler chickens. The expression of fatty acid synthase (FAS), apolipoprotein A-I (APOA-I), apolipoprotein B (APOB), adiponectin (ADIPOQ), liver kinase B1 (LKB1), and AMP-activated protein kinase α-1 (AMPKα-1) genes was evaluated by qRT-PCR. In Experiment 1, we observed a gender effect on feed intake, as male broilers presented greater feed intake than females. Female broilers presented greater gene expression of FAS, and lower expression of ADIPOQ and AMPKα-1, than males. A gender effect was not observed for the gene expression of APOA-I, APOB, or LKB1. In Experiment 2, there was a significant age effect on feed intake and weight gain. Broilers 42 days of age presented greater feed intake and weight gain than 21-day-old birds. 21-day-old broilers showed greater expression of APOA-I, ADIPOQ, LKB1, and AMPKα-1, and lower APOB gene expression in the liver than 42-day-old broilers. Age had no effect on FAS gene expression. Our results show that the gender and age could act on the expression of genes related to lipid synthesis, such as FAS and APOB, and also on genes related to lipid oxidation, such as ADIPOQ, LKB1, and AMPK.

Effect of an acidifier as replacement for antibiotics on the performance and gut morphology of broilers

2015 ◽  
Vol 11 (2) ◽  
pp. 111-116
Author(s):  
BA Makanjuola ◽  
OO Obi ◽  
TO Olorungbohunmi ◽  
OA Morakinyo ◽  
BA Boladuro ◽  
...  
Keyword(s):  
Drinking Water ◽  
Weight Gain ◽  
Feed Intake ◽  
Broiler Chickens ◽  
Broiler Chicks ◽  
Villus Height ◽  
Gut Morphology ◽  
Final Weight ◽  
Small Intestines ◽  
Dietary Treatments

An experiment was conducted to determine the influence of an acidifier as an alternative to antibiotics on the performance and gut morphology of broiler chickens. One hundred and eighty (180) 7-day old broiler chicks were randomly assigned to 3 dietary treatments of 4 replicates each. Each replicate had 15 birds. The diets fed to the birds were: Treatment 1- diet with neither antibiotic nor acidifier; Treatment 2- diet with antibiotic and Treatment 3- diet with acidifier. The antibiotic used was Enrofloxacin which was administered to the birds on Treatment 2 via their drinking water while the acidifier used was Biotronic SE (a combination of propionic and formic acids) which was added to the diet of birds on Treatment 3 at the rate of 4kg/tonne of feed. The experiment lasted 42 days. No significant differences were observed in the feed intake, final weight and weight gain of the birds on the different diets. Significant differences were however observed in the villus height in the duodenum and ileum of the birds. The crypt depth values in the different segments of the small intestines of the birds were not significantly affected by the different treatments.Key words: Organic acids, Biotronic SE, Enrofloxacin, Crypt, villus.

scholarly journals Growth performance, blood parameters and production cost of broiler chickens fed dietary sweet orange peel meal diets with and without enzyme addition

2020 ◽  
Vol 46 (1) ◽  
pp. 37-50
Author(s):  
T. A. Sunmola ◽  
C. D. Tuleun ◽  
O. I. A. Oluremi
Keyword(s):  
Weight Gain ◽  
Growth Performance ◽  
Feed Intake ◽  
Broiler Chickens ◽  
Production Cost ◽  
Sweet Orange ◽  
Control Diet ◽  
Orange Peel ◽  
Blood Parameters ◽  
Final Weight

The growth performance, blood parameters and production cost of two hundred and fifty two (252) Abor acre plus broiler chickens fed sweet orange peel meal (SOPM) with and without enzymes were investigated. The experimental diets consist of control (T ), maize was 0 replaced with SOPM at 15, 20 and 25 % without exogenous enzymes to form T , T and T 1 2 3 respectively, and another supplemented with exogenous enzyme to form T , T and T 4 5 6 respectively. The birds were randomly allocated to seven treatment groups replicated three times to have 12 birds per replicate and were fed experimental diet ad-libitum for eight weeks. Result showed that broiler chickens fed control diet (T ) had significantly (P<0.05) 0 higher final weight (FW) of 2.44kg, weight gain (WG) of 42.91, feed intake (FI) of 86.53, better feed conversion ratio (FCR)of 2.01 and protein conversion ratio (PCR)of 0.44 compared to other dietary treatments. Broiler chickens fed T and T had significantly 1 4 (P<0.05) higher final weight of 1.98 and 1.98 kg, weight gain of 34.69 and 34.71 g and feed intake of 78.35 and 81.63 g respectively while significantly (P<0.05) least values of 1.75 and 1.80 kg final weight, 30.73 and 31.49 g weight gain and 69.47 and 71.84 g feed intake respectively were recorded in broiler chickens fed T and T . Haematological indices namely: 2 5 PCV, RBC, WBC and Hb were not significantly different (P>0.05) but MCV, MCH, MCHC, lymphocytes, heterophil, eosinophil and monocytes differed significantly (P<0.05). Serum biochemical indices namely total protein, albumin, globulin and uric acid were significantly (P<0.05) influenced by the experimental diets. Though, there was no detrimental effect of SOPM and enzyme on production cost as profit was made at all replacement levels of SOPM with and without enzyme treatment, but profit per bird (N) and cost to benefit ratio revealed the superiority of the broiler chickens fed control diet (T ). Based on the results obtained, 0 SOPM can replace maize up to 25 % without posing any deleterious effect on the broiler chickens growth performance, blood parameters and production cost. However, the cost to benefit ratio was compromised with the use of SOPM. 

Performance, haematology and carcass characteristics of broiler chickens given water from different sources

2021 ◽  
Vol 39 (1) ◽  
pp. 104-113
Author(s):  
O. R Folorunso ◽  
E. A. O. Laseinde ◽  
G. E. Onibi
Keyword(s):  
Weight Gain ◽  
Water Intake ◽  
Feed Intake ◽  
Broiler Chickens ◽  
Stream Water ◽  
Relative Weight ◽  
Carcass Characteristics ◽  
Daily Weight Gain ◽  
Borehole Water ◽  
Different Sources

n eight week study was conducted to assess the performance, carcass characteristics and haematological indices of broiler chickens provided water from different sources. One hundred and fifty day - old broiler chicks (Arbor acre) were randomly allocated to five treatment groups (rain, stream, pipe borne water, well and borehole water respectively) in a completely randomized experimental design. The borehole water was obtained from a residential building, about 500 m to the experimental site (Federal College of Agriculture (FECA), Akure, Nigeria). Rainwater was harvested when it rained and was stored in plastic containers before it was used. It was not stored for more than three days before use. Stream water was obtained from a stream that flowed close to the students' residential area of FECA. It is sometimes drunk and is typical of streams that flow in a typical village community. Well water was obtained from a well sunk within the premises of the FECA. It was a well from where people fetched water for domestic use and was provided to the birds without prior treatment. Pipe borne water was obtained from the Water Corporation Mains that supplies FECA and Ijapo Estate, Akure. Results from the experiment revealed that there were no significant (P>0.05) differences among the treatments in terms of water intake, feed intake, weight gain, feed conversion ratio, carcass characteristics, relative weight of some organs, muscle dimensions and haematological parameters. Pipe borne water had the highest numerical values (P>0.05) for most of the performance and carcass characteristic indices measured, such as daily water intake (181.53±14.6), cumulative water intake (8.90±.71), daily feed intake (106.27±4.15), cumulative feed intake (5.21±0.20), daily weight gain (38.53±2.38), total weight gain (1.89±0.12), final weight gain (1.98±0.12), percent dressed weight (97.11±0.16%), percent eviscerated weight (81.86±3.26%), relative weights of drumstick (99.37±5.43g/kg), wings (89.10±9.69g/kg), chest (170.08±6.59g/kg) and back (159.40±18.90g/kg live weight) respectively. Water from different sources (rain, well, pipe borne water, borehole and stream) had no significant (P>0.05) effect on the performance indices, considered in the study for broiler chickens. This implies that the aforementioned water sources can be used to raise broiler chickens provided such water is free from any contaminants that could impair the health of the birds.

Performance and slaughter characteristics of broiler chickens fed different sources of maggot meal

2020 ◽  
Vol 44 (2) ◽  
pp. 262-270
Author(s):  
O. O. Egbewande ◽  
A. A. Jimoh ◽  
B. Y. Amore ◽  
B. Y. Amore
Keyword(s):  
Weight Gain ◽  
Feed Intake ◽  
Broiler Chickens ◽  
Fish Meal ◽  
Body Weight Gain ◽  
Proximate Analysis ◽  
Feed Conversion ◽  
Performance Indices ◽  
Randomized Design ◽  
Different Sources

In this study, the effect of three different sources of maggot meals was conducted to evaluate the performance and slaughter characteristics of broiler chickens. Five diets were formulated to include Diet 1 (control) which contained 3.5 % fish meal, while Diets 2, 3 and 4 contained 3.5% maggot meal from broiler droppings (BMM),maggot meal from layers droppings (LMM) and maggot meal from rabbit faeces (RMM) respectively. Eighty (80) dayold unsexed Marshall Broilers were randomly allotted to four dietary treatments in a completely randomized design (CRD). Thedietary treatments had 20 birds each with two (2) replicates. Data were generated on proximate analysis and performance indices including feed intake and weight gain, slaughter characteristics and mortality rate. They were subjected to analysis of variance (ANOVA) and means significantly different were separated using Duncan's Multiple Range Test (DMRT) at 5% probability level. Results from proximate analysis showed that maggots from layer droppings had the highest crude protein and ether extract. Results on performance indices showed that birds fed on diet 3 (layer maggot meal) had significantly (P<0.05) best performance than others as regards feed intake. Layer maggot meal (LMM) in this study enhanced bestproductive performance compared to those from broiler and rabbit maggot.Birds fed layer maggot meal had the best (1.85) feed conversion ratio. They also had the best body weight gain (2501g) and feed intake (4627g). In conclusion, layer maggot meal is recommended as analternative animal protein source to expensive fish meal.

Performance of broiler chickens fed graded dietary inclusion levels of moringa (Moringa oleifera) seed cake

2020 ◽  
Vol 47 (2) ◽  
pp. 107-114
Author(s):  
M. Afolayan ◽  
M. M. Iliya ◽  
G. S. Bawa ◽  
L. Alayande
Keyword(s):  
Weight Gain ◽  
Feed Intake ◽  
Broiler Chickens ◽  
Moringa Oleifera ◽  
Carcass Characteristics ◽  
Daily Weight Gain ◽  
Poultry Feed ◽  
Seed Cake ◽  
Final Weight ◽  
Dietary Treatments

The progressive increase in the cost and scarcity of conventional feedstuffs, especially energy and protein sources has resulted into a continuous effort in researching into the possible utilization of the under - exploited wild legumes in poultry feed formulation. Several Researchers had reported on the utilization of Moringa oleifera leaf meal in poultry diets whereas; few reports exist on the utilization of Moringa seed. Therefore, this study wasconducted to evaluate the effect of graded dietary levels of moringa seed cake (MSC) on the performance of broiler chickens. A total of 195, one day old Arbor Acre broiler chicks were randomly assigned into five dietary treatments of moringa seed cake, each treatment having three replicates in a completely randomized design. MSC was included at levels of 0, 5,10, 15 and 20% respectively. Feed and water were given ad- libitum. Proximate composition ofMSC showed that it is high in energy, crude protein and ether extract with3304. 39 ME kcals/ kg, 32.49 % and 17.70%, respectively. Birds fed 5% MSC diet were better than the birds on the other levels of MSC diets in terms of growth and carcass characteristics. The results obtained from growth study revealed a significant declined in, final weight, weight gain, feed intake, and feed conversion ratio as the inclusion levels of MSC increased beyond 5% in thediets. The birds on control diet had the best result in terms of final weight , daily weight gain and daily feed intake of 2974g/bird, 52.4g/bird / day and 107.51g/bird/ day respectively followed by those fed diet containing 5% MSC with 2183.4g/bird, 38.24/bird /day and 95.66g/bird /day respectively. whereas, the birds fed 20% MSC had the least (P>0.05) final weight, daily weight gain and daily feed intake of 560 g/bird, 9.26g/bird /day and 33.51g/bird/day respectively. Carcass characteristics followed similar trend as the growth parameters. Hematological indices showed significant differences across the dietary treatments, however, the results were within the normal range except for the white blood cell. It can therefore be, concluded that the inclusion of MSC in broiler chicken diet should not exceed 5% for optimum growth and general performance. 

scholarly journals SCAP/SREBPs are Central Players in Lipid Metabolism and Novel Metabolic Targets in Cancer Therapy

2018 ◽  
Vol 18 (6) ◽  
pp. 484-493 ◽  
Author(s):  
Xiang Cheng ◽  
Jianying Li ◽  
Deliang Guo
Keyword(s):  
Lipid Metabolism ◽  
Cancer Therapy ◽  
Transcriptional Activation ◽  
Molecular Mechanisms ◽  
Regulatory Element ◽  
Lipid Synthesis ◽  
Feedback Regulation ◽  
Negative Feedback Regulation ◽  
Expression Of Genes ◽  
New Findings

Lipid metabolism reprogramming emerges as a new hallmark of malignancies. Sterol regulatory element-binding proteins (SREBPs), which are central players in lipid metabolism, are endoplasmic reticulum (ER)-bound transcription factors that control the expression of genes important for lipid synthesis and uptake. Their transcriptional activation requires binding to SREBP cleavageactivating protein (SCAP) to translocate their inactive precursors from the ER to the Golgi to undergo cleavage and subsequent nucleus translocation of their NH2-terminal forms. Recent studies have revealed that SREBPs are markedly upregulated in human cancers, providing the mechanistic link between lipid metabolism alterations and malignancies. Pharmacological or genetic inhibition of SCAP or SREBPs significantly suppresses tumor growth in various cancer models, demonstrating that SCAP/SREBPs could serve as promising metabolic targets for cancer therapy. In this review, we will summarize recent progress in our understanding of the underlying molecular mechanisms regulating SCAP/SREBPs and lipid metabolism in malignancies, discuss new findings about SREBP trafficking, which requires SCAP N-glycosylation, and introduce a newly identified microRNA-29-mediated negative feedback regulation of the SCAP/SREBP pathway. Moreover, we will review recently developed inhibitors targeting the SCAP/SREBP pathway for cancer treatment.

scholarly journals Lipid Stores and Lipid Metabolism Associated Gene Expression in Porcine and Bovine Parthenogenetic Embryos Revealed by Fluorescent Staining and RNA-seq

2020 ◽  
Vol 21 (18) ◽  
pp. 6488
Author(s):  
Arkadiusz Kajdasz ◽  
Ewelina Warzych ◽  
Natalia Derebecka ◽  
Zofia E. Madeja ◽  
Dorota Lechniak ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lipid Metabolism ◽  
Inner Cell Mass ◽  
Mammalian Species ◽  
Cell Mass ◽  
Blastocyst Stage ◽  
Preimplantation Embryos ◽  
Rna Seq ◽  
Expression Of Genes ◽  
Bovine Blastocyst

Compared to other mammalian species, porcine oocytes and embryos are characterized by large amounts of lipids stored mainly in the form of droplets in the cytoplasm. The amount and the morphology of lipid droplets (LD) change throughout the preimplantation development, however, relatively little is known about expression of genes involved in lipid metabolism of early embryos. We compared porcine and bovine blastocyst stage embryos as well as dissected inner cell mass (ICM) and trophoblast (TE) cell populations with regard to lipid droplet storage and expression of genes functionally annotated to selected lipid gene ontology terms using RNA-seq. Comparing the number and the volume occupied by LD between bovine and porcine blastocysts, we have found significant differences both at the level of single embryo and a single blastomere. Aside from different lipid content, we found that embryos regulate the lipid metabolism differentially at the gene expression level. Out of 125 genes, we found 73 to be differentially expressed between entire porcine and bovine blastocyst, and 36 and 51 to be divergent between ICM and TE cell lines. We noticed significant involvement of cholesterol and ganglioside metabolism in preimplantation embryos, as well as a possible shift towards glucose, rather than pyruvate dependence in bovine embryos. A number of genes like DGAT1, CD36 or NR1H3 may serve as lipid associated markers indicating distinct regulatory mechanisms, while upregulated PLIN2, APOA1, SOAT1 indicate significant function during blastocyst formation and cell differentiation in both models.

scholarly journals Effect of stocking density on performance, carcass yield, productivity, and bone development in broiler chickens Cobb 500®

2017 ◽  
Vol 38 (4Supl1) ◽  
pp. 2705
Author(s):  
Cleoneide Dos Santos Henrique ◽  
Andréia Fróes Galuci Oliveira ◽  
Thales Silva Ferreira ◽  
Eduardo Santos Silva ◽  
Bruna Finotti Fonseca Reis de Mello ◽  
...  
Keyword(s):  
Weight Gain ◽  
Feed Intake ◽  
Broiler Chickens ◽  
Bone Development ◽  
Stocking Density ◽  
Live Weight ◽  
Feed Conversion ◽  
Randomized Design ◽  
Total Phase ◽  
Completely Randomized Design

This study aimed to assess the effect of stocking density of 10, 12, 14, and 16 birds m?2 on performance, carcass and cut yields, productivity, and bone development of broiler chickens Cobb 500®. The experimental design was completely randomized design with four treatments (stocking densities) and four replications. The assessments were performed weekly at 1, 7, 14, 21, 28, 35, and 42 days. The best live weight result was found at a density of 10 birds m?2 in the period from 1 to 7 days. No effect of density was observed on feed intake and weight gain. From 1 to 14 days, the best live weights were found at densities of 10, 12, and 14 birds m?2, in addition to a higher feed intake at densities of 10 and 12 birds m?2. Weight gain was better at a density of 10 birds m?2, showing no differences from densities of 12 and 14 birds m?2. In the period from 1 to 21 days, live weight and weight gain were higher at densities of 10, 12, and 14 birds m?2. The highest feed intake was observed at a density of 10 birds m?2, which showed no difference from the density of 14 birds m?2. A difference (P < 0.05) was observed for live weight, weight gain, and feed intake of animals reared at densities of 10, 12, and 14 birds m?2 for the phases from 1 to 35 days and the total phase from 1 to 42 days of bird age when compared to 16 birds m?2, which presented lower performance. However, feed conversion of 1-7, 1-14, 1-21, 1-35, and 1-42 days and carcass and cut characteristics showed no significant differences for all treatments. On the other hand, productivity was higher in the treatments with 14 and 16 birds m?2, but without difference between each other. No interaction (P > 0.05) was observed between stocking density and age for the assessed bone parameters. Similarly, no difference (P > 0.05) was observed for bone development of broiler chickens reared at different stocking densities for the studied parameters. Thus, the increased density of 10 or 12 birds m?2 to 14 birds m?2 has no influence on zootechnical performance, carcass and cut yields, and bone development, but increases broiler chicken productivity reared in a conventional shed without air conditioning.

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