scholarly journals Callus induction and regeneration efficiency of spring barley cultivars registered in the Czech Republic

2011 ◽  
Vol 50 (No. 10) ◽  
pp. 456-462 ◽  
Author(s):  
V. Šerhantová ◽  
J. Ehrenbergerová ◽  
L. Ohnoutková
Keyword(s):  
Czech Republic ◽  
Callus Induction ◽  
Callus Formation ◽  
Spring Barley ◽  
Zygotic Embryos ◽  
Golden Promise ◽  
The Czech Republic ◽  
Barley Cultivars ◽  
Regeneration Efficiency

Immature zygotic embryos are frequently used for induction of cell cultures in vitro and for genetic transformation. The effect of three synthetic auxins: 2,4-dichlorophenoxyacetic acid (2,4-D), 3,6-dichloro-2-methoxybenzoic acid (dicamba) and 4-amino-3,5,6-trichloropicolinic acid (picloram) on induction and subsequent regeneration capacity of barley. In vitro culture was studied in 12 spring barley cultivars registered in the Czech Republic and a variety of Golden Promise, with known high regeneration efficiency. The callus formation frequency and number of green regenerants were influenced significantly both by genotype and auxin. Most cultivars formed statistically significantly a higher mean number of regenerants (1.95) after the callus induction on the medium with 2.4-D as compared to the media with picloram and dicamba. Only two cultivars (Nordus and SG-S-261) did not respond to the used auxins differently. The highest average number of regenerants (from all three auxins) was obtained with Golden Promise (2.7 plants per one cultivated scutellum). From the set of genotypes used in our study, the cultivars Atribut, Forum, and Scarlett with the mean number of regenerants (2.09–1.57) would be the most suitable cultivars for further transformation use. They differ statistically significantly from the cultivars Nordus, Amulet, Akcent, SG-S-252, Orbit, and Granát (0.42–0.92) which had the lowest mean number of regenerants.

scholarly journals Virulence frequencies to powdery mildew resistance genes of winter barley cultivars

2010 ◽  
Vol 40 (No. 4) ◽  
pp. 135-140 ◽  
Author(s):  
A. Dreiseitl
Keyword(s):  
Czech Republic ◽  
Powdery Mildew ◽  
Resistance Genes ◽  
Powdery Mildew Resistance ◽  
Spring Barley ◽  
Winter Barley ◽  
The Czech Republic ◽  
Mildew Resistance ◽  
Barley Cultivars ◽  
Random Samples

The virulence frequencies to powdery mildew resistance genes possessed by winter barley cultivars registered and newly tested in the Czech Republic were studied in 2000, 2002 and 2004. Random samples of the populations originating from winter and spring barley fields were obtained from the air by a mobile version of a jet spore sampler mounted on a car roof. Conidia were sampled by driving across the Czech Republic. Fourteen differentials, carrying 18 out of 20 currently identified resistance genes present in winter barley cultivars, were used. High virulence frequencies (85–100%) to most resistance genes were found. Lower virulence frequencies (14.1–40.1%) were found to only three resistance genes that have not been described yet; their preliminary designations are Ml(Va), Ml(Dt) and Ml(Ca). The importance of resistance of winter barley cultivars is discussed with respect to limiting the speed with which the pathogen adapts to genetic resistances possessed by commercial cultivars of both winter and spring barley, and to the necessity of lowering the costs for powdery mildew control in barley.

scholarly journals Changes in the population of Blumeria graminis f.sp. hordei in the Czech Republic from 2009 to 2010

2012 ◽  
Vol 47 (No. 2) ◽  
pp. 43-51 ◽  
Author(s):  
A. Dreiseitl
Keyword(s):  
Czech Republic ◽  
Powdery Mildew ◽  
Spring Barley ◽  
Blumeria Graminis ◽  
The Czech Republic ◽  
Barley Cultivars ◽  
Wide Range ◽  
Pathogen Populations

Virulences to powdery mildew resistances in barley cultivars mostly carrying unknown resistances were determined in 2009 and 2010. Random spore samples of the airborne pathogen populations originating from winter and spring barley fields were obtained by means of a mobile version of a jet spore sampler by travelling across theCzech Republic. In total 301 isolates were studied, 55 differentials carrying mostly unknown resistances were used and 80 pathotypes were found, of which 26 representing 73.1% of isolates were detected in both years. Virulence frequencies showed a wide range from 0% to 100%. Complexity of the 2010 population slightly increased, mostly due to increasing frequencies of virulence to new resistances, whereas the complexity of virulences to resistances in most other differentials decreased. Pathotype 00027 was the most abundant (10.0%). Diversity of the 2010 population  considerably increased due to changes in virulence frequencies.

Response of spring barley cultivars to Ramularia leaf spot in conditions of the Czech Republic

2013 ◽  
Vol 41 (1) ◽  
pp. 126-132 ◽  
Author(s):  
P. Matušinsky ◽  
M. Hanusová ◽  
L. Stemberková ◽  
P. Mařík ◽  
V. Minaříková ◽  
...  
Keyword(s):  
Czech Republic ◽  
Leaf Spot ◽  
Spring Barley ◽  
The Czech Republic ◽  
Barley Cultivars ◽  
Ramularia Leaf Spot

scholarly journals Virulence frequency to powdery mildew resistances in winter barley cultivars

2009 ◽  
Vol 44 (No. 4) ◽  
pp. 160-166 ◽  
Author(s):  
A. Dreiseitl
Keyword(s):  
Czech Republic ◽  
Powdery Mildew ◽  
Spring Barley ◽  
Winter Barley ◽  
The Czech Republic ◽  
Barley Cultivars ◽  
Random Samples ◽  
Wide Range ◽  
The Given ◽  
Lower Population

Virulence frequencies to powdery mildew resistances in winter barley cultivars mostly registered in the Czech Republic were studied in 2007 and 2008. Random samples of the air populations originating from winter and spring barley fields were obtained by means of a mobile version of a jet spore sampler. Conidia were sampled by driving across the Czech Republic. In total 349 isolates were studied and 17 differentials were used. The virulence frequencies to specific resistances of given cultivars showed wide range from 0% to 100%. Nine differentials were used to distinguish 134 pathotypes, of which 32 representing 63.9% of isolates were detected in both years. Pathotype 773, which broke down the resistance of eight differentials, was the most abundant. In 2008, lower virulence frequencies to all differentials, and thus lower population complexity, were determined, which may be caused by different regional origins of the isolates examined. Importance of the study of the given pathogen population is discussed in terms of successful breeding of resistant barley cultivars.

Potential of water balance and remote sensing-based evapotranspiration models to predict yields of spring barley and winter wheat in the Czech Republic

2021 ◽  
Vol 256 ◽  
pp. 107064
Author(s):  
František Jurečka ◽  
Milan Fischer ◽  
Petr Hlavinka ◽  
Jan Balek ◽  
Daniela Semerádová ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Czech Republic ◽  
Water Balance ◽  
Winter Wheat ◽  
Spring Barley ◽  
The Czech Republic

scholarly journals Callus Induction from Cotyledon of Gyrinops versteegii (Gilg.) Domke

2021 ◽  
Vol 21 (2) ◽  
pp. 427
Author(s):  
Fajri Marisa ◽  
Lisna Hidayati ◽  
Aries Bagus Sasongko ◽  
Tri Rini Nuringtyas
Keyword(s):  
Natural Population ◽  
Callus Induction ◽  
Callus Formation ◽  
Economic Value ◽  
Sustainable Utilization ◽  
Endemic Plant ◽  
High Quality ◽  
Bright Color ◽  
Significant Opportunity

Gyrinops versteegii is an endemic plant in eastern Indonesia that produced agarwood with high quality and economic value. This plant has been threatened by overexploitation which leads on decreasing in the natural population. This research aimed to induce in vitro callus formation to support the sustainable utilization of G. versteegii, which in the end, may support plant productivity. Callus induction was investigated using cotyledon and cultured on MS medium supplemented with several combination of plant growth regulators. The observations were done for the number of explants turned into callus and the duration for the first callus formation. The results showed that combination of 3 mg/L NAA + 0.5 mg/L BAP was recorded as the best combination for callus induction (63.63%). Callus with friable structure and bright color are obtained within nine days of incubation and showed the characteristic of embryonic callus. This result is expected to give a significant opportunity to conserve the natural population of G. versteegii.

scholarly journals In vitro Callus Induction and Regeneration of Popular Indica Rice Genotypes

2020 ◽  
Vol 13 (4) ◽  
Author(s):  
Aananthi. N
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
Indica Rice ◽  
Immature Embryo ◽  
Coconut Milk ◽  
Rice Genotypes ◽  
Mist Chamber ◽  
Regenerated Plant ◽  
Better Than

Five rice cultivars viz., ASD 16, White Ponni, Pusa Basmati 1, Pusa Sugandh 4 and Pusa Sugandh 5 belonging to subspecies indica were compared for its ability in callus formation and regeneration. In this experiment, the different parameters viz., the effect of hormones (2,4-D and kinetin), organic supplement (coconut milk O1-CM 100 mll-1, O2-CM 75 mll-1, O3-CM 50 mll-1), explants (seed and immature embryo), media (MS and N6), carbon source (sucrose and maltose) using five genotypes on callus response was studied. The effect of hardening methods was also assessed. Results showed that for enhanced callus induction was with MS medium supplemented with 2.0 mgl-1 2, 4-D + 0.5 mgl-1 kinetin + 30 gl-1 maltose irrespective of explants used. Addition of 100 ml l-1 coconut milk was found have improvement in callus response. The performance of immature embryo was better than seed for callus induction, emrbyogenic callus formation, rhizogenic callus formation and regeneration. MS media provided superiority over N6. Among the genotypes Pusa Basmati 1 rendered outstanding performance in callus behavior. The treatment combination MS + 2.5 mgl-1 BAP + 0.5 mgl-1 NAA + 1.0 mgl-1 KN gave the highest organogenesis response and regeneration of plantlets. Hardening in mist chamber was recognized as the best method to give the highest per cent of regenerated plant lets.

scholarly journals Callus Induction in Baru (Dipteryx alata Vog.) Explants

2018 ◽  
Vol 47 (2) ◽  
pp. 538-543
Author(s):  
Rodrigo Kelson S. REZENDE ◽  
Ana Maria N. SCOTON ◽  
Maílson V. JESUS ◽  
Zeva V. PEREIRA ◽  
Fernanda PINTO
Keyword(s):  
Ascorbic Acid ◽  
Callus Induction ◽  
Callus Formation ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Alternative Technique ◽  
Propagation Methods ◽  
Dipteryx Alata

Baru (Dipteryx alata Vog.) is a species with great economic and environmental potential; it has popular acceptance, besides being a very productive species. Alternative propagation methods are important for species maintenance and exploration. Thus, micropropagation emerged as an alternative technique, providing genetic stability and the production of a large number of seedlings. The aim of the present investigation was to develop a callus induction protocol for in vitro baru explants. The tested explants were nodal, internodal and foliar segments. The explants were disinfected for 30 seconds in 70% alcohol (v/v) and 2 minutes in sodium hypochlorite (1.25% active chlorine). This was followed by triple washing. The inoculation was carried out in test tubes containing 15 mL MS medium (30 g L-1 sucrose, 6 g L-1 agar and 100 mg L-1 ascorbic acid) supplemented with 2.0 mg L-1 naphthalene acetic acid (NAA). The solution also contained 0.0, 2.5 or 5.0 mg L-1 of 6-benzylaminopurine (BAP) with the pH adjusted to 5.8. In the incubation phase, the explants were cultured for seven days in the dark and then subjected to a photoperiod of 16 hours (43 µmol m-2 s-1) at 25 ± 2 °C. The treatments were studied with 2.5, 5.0, 7.5 or 10.0 mg L-1 BAP additions to the MS. Callus formation, contamination and oxidation evaluations were undertaken. The results obtained when using 2.0 mg L-1 NAA concluded that such a treatment should be used to induce callogenesis from nodal explants, while for the tested baru leaf explants, the best results for callus formation were given by the combination of 2.0 mg L-1 NAA with 2.5 mg L-1 of BAP to.

Sign in / Sign up

Export Citation Format

Share Document