scholarly journals Comparison of in vitro gas production technique with in situ nylon bag technique to estimate dry matter degradation

2011 ◽  
Vol 50 (No. 2) ◽  
pp. 60-67 ◽  
Author(s):  
A. Kamalak ◽  
O. Canbolat ◽  
Y. Gurbuz ◽  
O. Ozay
Keyword(s):  
Dry Matter ◽  
Degradation Kinetics ◽  
Insoluble Fraction ◽  
Gas Production ◽  
Barley Straw ◽  
Production Technique ◽  
In Vitro Gas Production ◽  
Dry Matter Degradation

Dry matter (DM) degradation of wheat straw (WS), barley straw (BS), lucerne hay (LH) and maize silage (MS) was determined using two different techniques: (i) in vitro gas production and (ii) nylon bag degradability technique. In vitro gas production and in situ DM disappearance were measured after 3, 6, 12, 24, 48, 72 and 96 hours of incubation. In situ and in vitro DM degradation kinetics was described using the equation y = a + b (1 &ndash; e<sup>ct</sup>). In all incubations there were significant (P &lt; 0.001) correlations between gas production and in situ DM disappearance or estimated parameters ((a + b)<sub>ga</sub><sub>s</sub> and (a + b)<sub>is</sub> or (a + b)<sub>gas</sub> and EDMD<sub>is</sub>) whereas there were no significant (P &gt; 0.05) correlations between c<sub>gas</sub> and c<sub>is</sub> or b<sub>gas</sub> and b<sub>is</sub>. Gas production from the insoluble fraction (b) alone explained 98.3% of the variation of EDMD. The inclusion of gas production from the quickly soluble fraction (a) and rate constant (c) of gas production in the regression equation improved the accuracy of EDMD prediction. The correlations between the results of both methodologies seem to be sufficiently strong to predict degradability parameters from gas production parameters. It was concluded that the in vitro gas production technique has good potentiality to predict in situ DM disappearance and some DM degradation parameters. &nbsp; &nbsp;

Comparison between in vitro gas production and in situ degradation for evaluating rumen dry matter degradation of maize grain and tropical grasses

2006 ◽  
Vol 34 ◽  
pp. 69-72
Author(s):  
R. M. Mauricio ◽  
T.R. Tomich ◽  
R. A. Filho ◽  
R. B. Reis ◽  
L.C. Gonçalves ◽  
...  
Keyword(s):  
Closed System ◽  
Dry Matter ◽  
Gas Production ◽  
Production Technique ◽  
In Vitro Gas Production ◽  
Dry Matter Degradation ◽  
In Situ Degradation ◽  
Maize Grain

SummaryThe in situ technique (NB) was compared to the in vitro gas production technique (Gas) in terms of ability to estimate the dry matter degradation (DMD) using high soluble substrates (maize grains) and low soluble substrates (four tropical forages). The experiments (in situ and in vitro) were carried out at the same time using the same cow for both techniques and DMD was estimated at 6, 12, 24, 48 & 96 h. The results showed that DMD from Gas were lower than NB DMD and the correlation for the maize group were lower than those of the grass group which indicated that Gas technique, closed system, has potential to analyse highly soluble substrates, probably, overcoming the particle losses effect demonstrated by the in situ technique.

Inclusion of yeast waste as a protein source to replace soybean meal in concentrate mixture on ruminal fermentation and gas kinetics using in vitro gas production technique

2019 ◽  
Vol 59 (9) ◽  
pp. 1682
Author(s):  
Anusorn Cherdthong ◽  
Rittikeard Prachumchai ◽  
Chanadol Supapong ◽  
Benjamad Khonkhaeng ◽  
Metha Wanapat ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Dry Matter ◽  
Insoluble Fraction ◽  
Gas Production ◽  
Protein Source ◽  
Production Technique ◽  
Dry Matter Digestibility ◽  
In Vitro Gas Production ◽  
Concentrate Mixture

This experiment was conducted to investigate the utilisation of yeast waste as protein source to replace soybean meal in concentrate mixture on kinetic of gas, rumen ammonia-nitrogen and digestibility of nutrients by using in vitro gas production technique. The experimental design was a completely randomised design and the dietary treatments were replacing soybean meal with yeast wastein concentrate at the ratio of 100:0, 75:25, 50:50, 25:75 and 0:100, respectively. Yeast waste was obtained from KSL Green Innovation Public Co. Limited, Thailand. The gas production was recorded at 0, 0.5, 1, 2, 4, 6, 8, 12, 16, 24, 48, 72 and 96 h of incubation. The yeast waste contained 26.4% crude protein. Gas production from soluble fractions (a), gas production from the insoluble fraction (b), potential extent of gas production (a+b) and the gas production rate constants for the insoluble fraction (c) were not altered when increasing concentration of yeast waste replacing soybean meal (P &gt; 0.05). Cumulative gas production (at 96 h of incubation) ranged from 69.3 to 72.8 mL and was similar among treatments. Ruminal NH3-N concentration was linearly increased (P &lt; 0.05) whereas ruminal pH did not alter when inclusion various levels of yeast waste replacing soybean meal, which ranged from 15.2 to 19.1 mg/dL and 6.90 to 6.94, respectively. In vitro dry matter digestibility and in vitro organic matter digestibility did not changed by increasing levels of yeast waste in the diets (P &gt; 0.05), except only in vitro dry matter digestibility at 12 h, which higher in soybean meal:yeast waste at 25:75 ratio (P &lt; 0.05). Furthermore, propionate (C3) molar was linearly higher when compared between inclusion yeast waste and the control group whereas acetate was decreased quadratically (P &lt; 0.05) and protozoal population tended to be decreased (P = 0.07) when increasing the level of replacing yeast waste. In conclusion, yeast waste could replace soybean meal in concentrate mixture with no negative effect on gas kinetics, rumen fermentation and in vitro digestibility, and therefore its use in animal feeding would contribute to a reduction in environmental pollution.

scholarly journals The nutritional evaluation of forage-based mixed rations in New Zealand using an in vitro gas production technique. 1: analytical survey

2021 ◽  
pp. 1-6
Author(s):  
N.D. Meads ◽  
R. Tahmasbi ◽  
N. Jantasila
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Environmental Science ◽  
Ghg Emissions ◽  
Negative Relationship ◽  
Real Data ◽  
Gas Production ◽  
Production Technique ◽  
In Vitro Gas Production

Greenhouse gas (GHG) emissions from livestock are an important consideration in environmental science. Estimating GHG production can be problematic at a farm or animal level, and requires controlled conditions to produce real data. An in vitro gas production technique (IVGPT) was developed to evaluate forage-based total mixed rations in digestion kinetics and GHG production. Two hundred and sixty samples of complete mixed rations (MR), which included a pasture component used in commercial lactating dairy herds, were collected around NZ across three calendar years, 2017-2019. Twenty of the 260 samples were 100% total mixed rations (TMR) with no pasture content. The samples were submitted for proximate analysis as well as IVGPT to generate GHG production figures. The results showed an average total gas production (TGP) of 129.82 ml/g dry matter (DM), 78.6% true digestibility (TDMD), 125.06 mg/g DM microbial biomass (MB), 20.16 g CH4/kg DM, and 12.8 MJME/kg DM. The average nutrient composition was dry matter (DM) 31.55%, crude protein (CP) 21.85%, neutral detergent fibre (NDF) 44.35%, and starch 7.03%. The IVGPT CH4 production was negatively correlated to NDF (r=-0.312), ADF (r=-0.193), TGP (r=-0.216), and was positively correlated with TDMD (r=0.250), apparent digestibility (ADMD) (r=0.614), starch (r=0.117) and volatile fatty acids (r=0.538). The MR diet showed a strong positive relationship with ADMD digestibility (P=0.01) and a negative relationship with fibre content (NDF, P=0.01 and ADF, P=0.01). However, CH4 production reduced linearly with increasing TGP (P=0.01). The results indicated that a greater CH4 production may be related to higher digestibility of mixed ration.

Comparison of in situ and in vitro gas production techniques to estimate rumen degradation characteristics of forages

1998 ◽  
Vol 22 ◽  
pp. 247-248
Author(s):  
S. López ◽  
M. D. Carro ◽  
C. Valdés ◽  
J. S. González ◽  
F. J. Ovejero
Keyword(s):  
Gas Production ◽  
Degradation Kinetic ◽  
Production Methods ◽  
Rumen Degradation ◽  
In Vitro Gas Production ◽  
Dry Matter Degradation ◽  
Production Techniques ◽  
Kinetics Of

Quantitative expressions of the kinetics of digestion are needed to estimate more precisely the quantity and composition of nutrients digested from feeds and their subsequent efficiency of utilization by the animal (Mertens, 1993). Degradation kinetic parameters are estimated by fitting appropriate models to data resulting from the measurement of either the undigested residue or the fermentation end-products after exposure of the feed to digestion. Kinetic data can be collected using either in vitro or in situ procedures. The in situ porous synthetic fibre bag technique has been extensively used to describe the kinetics of feeds degradation in the rumen. Several in vitro gas production methods have been developed to assess fermentation kinetics. The aim of this work was to study the relationship between rumen degradation parameters of forages determined either by the in situ procedure or by the gas production technique and to evaluate the potential of gas production measurements to assess the extent of dry matter degradation in the rumen.

Prediction of DMD from gas production for substrates with high and low soluble carbohydrate content

2006 ◽  
Vol 34 ◽  
pp. 29-33
Author(s):  
Ú.T. Nogueira ◽  
R. M. Maurício ◽  
L. C. Gonçalves ◽  
N. M. Rodrigues ◽  
L. G. R. Pereira ◽  
...  
Keyword(s):  
Sugar Cane ◽  
Dry Matter ◽  
Gas Production ◽  
Carbohydrate Content ◽  
Lag Phase ◽  
Soluble Carbohydrate ◽  
Production Technique ◽  
Maize Silage ◽  
Dry Matter Degradation

SummarySugar cane and maize silage samples were evaluated by the semi automated in vitro gas production technique. The associations between gas production and dry matter degradation (DMD) were analysed and high coefficients of determination were obtained for all the substrates. The results showed that particles losses could be influencing the DMD, including errors mostly when obtained before 6 hours of incubation. The gas alone could better predict the lag phase than the DMD before 6 hours of incubation.

Rumen degradability and gas production as influenced by different strains of Bacillus thuringiensis

2019 ◽  
Vol 99 (4) ◽  
pp. 951-954
Author(s):  
F.C. Campos ◽  
A.L. Abdalla Filho ◽  
P.S. Corrêa ◽  
C. Nazato ◽  
R.G. Monnerat ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Dry Matter ◽  
Gas Production ◽  
Fibrobacter Succinogenes ◽  
Production Technique ◽  
Fermentation Processes ◽  
Ruminal Microorganisms ◽  
In Vitro Gas Production ◽  
Different Strains

The effect of six Bacillus thuringiensis (Bt) strains on diet degradability was evaluated using an in vitro gas production technique. Spores (5.7 × 106 spores) of different Bt strains (907, 1192, 2036, 2493, 2496, and S1185) plus a control (no spores) were used as treatments with four replicates (inocula) in duplicate. Fermentation processes were evaluated and ruminal microorganisms were quantified. Compared with the control, the Bt907 strain decreased dry matter (DM) and organic matter (OM) degradability without affecting the Fibrobacter succinogenes population, whereas the other strains reduced this population without altering DM and OM degradability.

scholarly journals Fermentation kinetics and in vitro degradation rates of grasses of the genus Cynodon

2016 ◽  
Vol 38 (3) ◽  
pp. 249 ◽  
Author(s):  
Sidnei Tavares dos Reis ◽  
Marcus Vinícius Gonçalves Lima ◽  
Eleuza Clarete Junqueira de Sales ◽  
Flávio Pinto Monção ◽  
João Paulo Sampaio Rigueira ◽  
...  
Keyword(s):  
Dry Matter ◽  
Soluble Fraction ◽  
Insoluble Fraction ◽  
Gas Production ◽  
Subsequent Reduction ◽  
Fermentation Kinetics ◽  
In Vitro Gas Production ◽  
Degradation Rates ◽  
Automated Technique

The present study aimed to evaluate the fermentation kinetics and degradation rates of Cynodon grasses estimated by semi-automated technique of in vitro gas production. The forages were: Coastcross, Tifton 85 and Tifton 68. Pressure readings were taken at 0, 2, 4, 6, 8, 10, 12, 15, 19, 24, 30, 36, 48, 72 and 96 hours. Dry matter degradability (DMD) was obtained by the percentage of dry matter (DM) remaining after 0, 6, 12, 24, 48 and 96 hours of fermentation. Tifton 85 showed a higher total gas production (p <0.05). Higher fermentation rates were found at the beginning of fermentation followed by subsequent reduction (p <0.05) over time. Tifton 85 and Tifton 68 showed higher values (p < 0.05) for soluble fraction, potentially degradable insoluble fraction, insoluble fraction, potential and effective degradability of dry matter in relation to Coastcross grass. Higher gas production during in vitro incubation of dry matter was observed for Tifton 85 g. 

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