scholarly journals Activity of glutathione peroxidase (GSH-Px) in the blood of ewes and their lambs receiving the selenium-enriched unicellular alga Chlorella

2008 ◽  
Vol 53 (No. 7) ◽  
pp. 292-298 ◽  
Author(s):  
J. Trávníček ◽  
J. Racek ◽  
L. Trefil ◽  
H. Rodinová ◽  
V. Kroupová ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Blood Serum ◽  
Whole Blood ◽  
Selenium Supplementation ◽  
Control Group ◽  
Organic Form ◽  
Pregnancy And Lactation ◽  
Average Activity ◽  
Positive Effect ◽  
Experimental Group

The effect of supplementation of selenium inorganic and organic forms on the activity of glutathione peroxidase (GSH-Px) was investigated for 7–8 months in three groups of ewes (by five animals each) and in born lambs. The basal feed ration contained 55 &mu;g Se, the ewes of experimental group E1 received a supplement of 180 &mu;g Se in sodium selenite, and the ewes of experimental group E2 were administered a supplement of 180 &mu;g selenium bound in the biomass of the alga <I>Chlorella</I>. Control group C was without selenium supplement. The ewes were in the stage of non-pregnancy, pregnancy and lactation during the experiment. The average number of lambs born per ewe was 1.0 in groups C and E1 and 1.8 in group E2. Both forms of selenium supplementation resulted in higher activity of GSH-Px in the whole blood, higher content of Se in the blood serum (<I>P</I> < 0.001) and milk of ewes (<I>P</I> < 0.001). Average activity of GSH-Px in the whole blood of ewes was as follows: C 697.9 ± 179.3; E1 1 147.4 ± 181.5; E2 1 056.1 ± 267.5 U/g Hb (<I>P</I> < 0.001). It reached the highest values in the 5<sup>th</sup> to 6<sup>th</sup> month of Se supplementation while the activity decreased after parturition in connection with lactation. Higher utilisation of Se from the organic form, compared to the inorganic form, was reflected in a higher content of Se in the milk of group E2 ewes (<I>P</I> < 0.05). The positive effect of selenium supplementation of ewes contributed to higher activity of GSH-Px in the whole blood of their lambs (<I>P</I> < 0.001) and higher Se concentration in the blood serum (<I>P</I> < 0.01). The organic form of Se (group E2) was more efficient in this case. Average activity of GSH-Px in the whole blood of lambs was as follows: C 434.1 ± 70.6; E1 1 031.6 ± 172.3 and E2 1 055.6 ± 235.1 U/g Hb.

scholarly journals Selenium content in the blood serum and urine of ewes receiving selenium-enriched unicellular alga Chlorella

2008 ◽  
Vol 52 (No. 1) ◽  
pp. 42-48 ◽  
Author(s):  
J. Travnicek ◽  
L. Pisek ◽  
I. Herzig ◽  
J. Doucha ◽  
J. Kvicala ◽  
...  
Keyword(s):  
Blood Serum ◽  
Post Partum ◽  
Average Concentration ◽  
Control Group ◽  
Selenium Content ◽  
Unicellular Alga ◽  
Organic Form ◽  
Pregnancy And Lactation ◽  
Positive Effect ◽  
Experimental Group

The effect of selenium supplementation in inorganic and organic form was investigated in three five-member groups of ewes and their lambs in the course of 7 months. The basal feed ration contained 55 &micro;g Se, ewes of experimental group E1 received a supplement of 180 &micro;g Se in sodium selenite, and ewes of experimental group E2 were applied a supplement of 180 &micro;g selenium bound in the biomass of the alga Chlorella. Control group C did not receive any selenium. The ewes were in the stage of non pregnant, pregnancy and lactation during the experiment. Average contents of Se in the blood serum of ewes were as follows: E1 114.2 &plusmn; 23.6; E2 103.1 &plusmn; 20.3; C 68.6 &plusmn; 16.8 &micro;g/l (<i>P</i> < 0.001). A decrease in serum Se was recorded in all groups in the last third of pregnancy and in the first week post partum. Average contents of urinary selenium contents were also higher in experimental groups: E1 25.4 &plusmn; 13.5; E2 18.7 &plusmn; 9.6; C 13.3 &plusmn; 4.5 &micro;g/l (<i>P</i> < 0.001). The positive effect of supplementation of Se to ewes was reflected in its higher average concentration in the blood serum of born lambs: E1 48.5 &plusmn; 7.3; E2 53.5 &plusmn; 3.4; C 30.3 &plusmn; 7.4 &micro;g/l (<i>P</i> < 0.05). The effect of selenium bound in Chlorella biomass was higher and persisted until 30 days of lamb age: E1 52.9 &plusmn; 3.4; E2 59.0 &plusmn; 7.0; C 35.5 &plusmn;5.8 &micro;g/l (<i>P</i> < 0.01). The average number of lambs born per ewe was 1.0 in group C and E1, and 1.8 in group E2.

scholarly journals Effect of a long-term peroral supplementation with sodium selenite and selenium lactate-protein complex on selenium status in goats and their kids

2009 ◽  
Vol 54 (No. 7) ◽  
pp. 324-332 ◽  
Author(s):  
L. Misurova ◽  
L. Pavlata ◽  
A. Pechova ◽  
R. Dvorak
Keyword(s):  
Glutathione Peroxidase ◽  
Protein Complex ◽  
Sodium Selenite ◽  
Selenium Concentration ◽  
Selenium Supplementation ◽  
Control Group ◽  
Glutathione Peroxidase Activity ◽  
Significant Difference ◽  
Average Activity

The aim of this study was to evaluate the effect of a long-term peroral selenium supplementation in the form of sodium selenite and selenium lactate-protein complex by comparing selenium concentrations and glutathione peroxidase activity in blood of goats and their kids as well as comparing selenium concentrations in goat colostrums. For the study, a total of 27 clinically healthy pregnant white shorthair goats were used. They were divided to three groups, i.e., the control group (C) without any selenium supplementation, sodium selenite group (E1) and selenium lactate-protein complex group (E2). For four months, experimental goats received 0.43 mg of selenium per animal per day in diet; goats from the control group were given 0.15 mg of selenium per animal per day. At the beginning of the experiment, goats of all groups showed an average selenium concentration of 96 &mu;g/l in whole blood. On the parturition day, samples of first colostrum from goats and heparinized blood from goats and kids were taken. In the control group (C), average blood selenium concentrations of 111.4 ± 33.5 &mu;g/l were observed on the parturition day. In both experimental groups, selenium concentrations were significantly higher (<I>P</I> < 0.05). Average selenium concentration in the sodium selenite group (E1) was 177.2 ± 34.8 &mu;g/l and in the group supplemented with selenium lactate-protein complex (E2) 159.0 ± 28.5 &mu;g/l. Average glutathione peroxidase (GSH-Px) activity in blood of control goats (C) was 581.9 ± 99.2 &mu;kat/l, in group E1 1 154.6 ± 156.2 &mu;kat/l and in group E2 1 011.6 ± 153.6 &mu;kat/l. GSH-Px activity in experimental groups was significantly higher (<I>P</I> < 0.05) as compared with the control group. Average selenium concentrations in colostrum was in the control group 40.1 ± 12.8 &mu;g/l, in E1 99.0 ± 29.9 &mu;g/l and in group E2 79.0 ± 17.7 &mu;g/l. Colostral selenium concentrations in experimental groups were significantly higher (<I>P</I> < 0.05) as compared with the control group. No significant difference in the monitored parameters was found between experimental groups. In kids of control mothers (kC), average selenium concentrations in blood on the parturition day were 62.4 ± 22.9 &mu;g/l; kids of mothers supplemented with sodium selenite (kE1) showed average selenium levels of 100.0 ± 31.2 &mu;g/l, and the average selenium concentration in kids of mothers receiving lactate-protein complex was 83.4 ± 20.1 &mu;g/l (kE2). Average GSH-Px activity in control kids (kC) was 402.1 ± 153.9 &mu;kat/l. Kids from kE1 showed average activity of GSH-Px 806.1 ± 254.9 &mu;kat/l and kids from group kE2 529.9 ± 119.8 &mu;kat/l. Statistically significant difference (<I>P</I> < 0.05) was found only between kC and kE1 which showed significantly higher selenium concentration and GSH-Px activity. The results of this study confirm that both forms of selenium administered in experimental groups (i.e., sodium selenite and selenium lactate-protein complex) had similar biological effect in goats. However, results obtained in kids indicate a better effect of supplementation with sodium selenite.

scholarly journals Dynamics of IgG in the blood serum of sheep with different selenium intake

2008 ◽  
Vol 53 (No. 5) ◽  
pp. 260-265 ◽  
Author(s):  
H. Rodinova ◽  
V. Kroupova ◽  
J. Travnicek ◽  
M. Stankova ◽  
L. Pisek
Keyword(s):  
Blood Serum ◽  
Control Group ◽  
Organic Form ◽  
Selenium Intake ◽  
Pregnancy And Lactation ◽  
Inorganic And Organic

An experiment was conducted to study the effect of supplementation of ewes with selenium in an inorganic and organic form on the level of IgG during infertility, pregnancy and lactation and in their offspring until two months of age. Control Group C (<I>n</I> = 5) was without Se supplementation. Experimental groups: E1 (<I>n</I> = 5) was supplemented with 180 &mu;g Na<sub>2</sub>SeO<sub>3</sub> per ewe/day, E2 (<I>n</I> = 5) received 180 &mu; Se per ewe/day organically bound in the biomass of an alga of the genus <I>Chlorella</I>. The average number of newborn lambs per lambing ewe was 1.0, 1.0 and 1.8 in Group C, E1 and E2, respectively. The supplementation did not influence either weight or growth of lambs. The content of IgG increased significantly in ewes in the last third of pregnancy regardless of Se supplementation while their postpartal decrease (<I>P</I> < 0.01) followed only in Group C. Lambs in Group C had a significantly lower level of IgG on Day 30 (<I>P</I> < 0.05) and 60 (<I>P</I> < 0.01) after birth especially in comparison with Group E2. The results document a lower level of immunity in the postpartal period of lambing ewes without Se supplementation and enhancement of postnatal proteosynthesis in the offspring of lambing ewes supplemented with the organic form of Se.

PREVENTION AND CORRECTION OF DISORDERS OF THE METABOLISM OF PIGS WITH THE USE OF NATURAL FEED ADDITIVES

2021 ◽  
Vol 1 (2) ◽  
pp. 189-194
Author(s):  
L.A. Nikanova ◽  
◽  
E.N. Kolodina ◽  
R.A. Rykov ◽  
◽  
...  
Keyword(s):  
Blood Serum ◽  
Total Bilirubin ◽  
Live Weight ◽  
Feed Additives ◽  
The Body ◽  
Control Group ◽  
Blue Green Algae ◽  
Adaptive Ability ◽  
Positive Effect ◽  
Experimental Group

This article presents the results of studies conducted on piglets with the inclusion of natural feed additives in the diet in order to prevent and correct metabolism. The first experimental group of piglets received a total diet consisting of fullfledged compound feed, a complex micro-feed product consisting of blue-green algae Spirulina, dihydroquercetin and organic iodine, the second experimental group additionally received Spirulina and dihydroquercetin. The introduction of these natural feed additives into the diet of piglets helped to increase the adaptive ability , correction and prevention of metabolic disorders in the body. In these studies, dacha feed supplements prevented hyperbilirubinemia. As a result, the concentration of total bilirubin in the blood serum of pigs of the first experimental group was 1% lower, in the second experimental group it was 13,7% lower than in the control group. They also had a positive effect on the functional state of the liver, as evidenced by a lower content of AlAT and AsAT activity in the blood serum compared to those in piglets of the control group with a close cholesterol-forming fraction. The animals of the experimental group were less ill and the safety of this group was 100%, in the control group it was 90%. The average daily increase in live weight of pigs for 90 days in the first experimental group was 1% higher than in the control group, and in the second experimental group it was 21,7% higher.

Biochemical parameters of blood of calves when using a probiotic based on the strain enterococcus faecium l-3

2020 ◽  
Vol 1 ◽  
pp. 88-92
Author(s):  
M.N. Lebedev ◽  
◽  
S.P. Kovalev ◽  
Keyword(s):  
Alkaline Phosphatase ◽  
Blood Serum ◽  
Alanine Aminotransferase ◽  
Enterococcus Faecium ◽  
Biochemical Parameters ◽  
Control Group ◽  
Amylase Level ◽  
Serum Amylase Level ◽  
Positive Effect ◽  
Experimental Group

In this paper, we studied the effect of a probiotic, based on the Enterococcus Faeci-um L-3 strain on 20 black-motley calves. The effect of the drug on their biochemical parameters of blood serum were studied. The authors found that this drug, which was fed to calves up to 45 days of age, had a positive effect on the biochemical parameters of blood and on the total animal organism.. So, at 14 days of age, blood serum parame-ters in calves, not receiving the drug, such as: bilirubin, alkaline phosphatase, alanine aminotransferase and aspartate aminotrans-ferase levels were significantly higher than in calves that received probiotic, which indi-cates possible dystrophic changes in the liv-er. Also, at 14 days of age in cows, reciev-ing the drug , the blood serum amylase level was significantly higher than of the calves, not receiving the drug. By the age of 30 days, the tendency to a higher content of bilirubin, alkaline phosphatase, alanine ami-notransferase and aspartate aminotransferase in the blood serum, persisted in the calves of the control group, compared with the ani-mals of the experimental group. By the age of 45 days, the levels of bilirubin, alanine aminotransferase and alkaline phosphatase were also significantly higher in control group of animals, than in experimental group and were 5.0 ± 0.56 μmol / L and 3.72 ± 0.4 μmol / L , 30.66 ± 2.9 IU / L and 16.52 ± 1.2 IU / L, 345.7 ± 30.7 and 226.6 ± 24.1, respectively.The results obtained, indicated that regular feeding of a probiotic based on Enterococcus Faecium L-3 to calves of the milky period, contributes to the normaliza-tion of biochemical parameters and greater resistance to gastrointestinal disorders.

Соматические дисфункции у детей с задержкой речи

2017 ◽  
pp. 61-65
Author(s):  
E. L. Kuznetsova ◽  
L. A. Dultsev ◽  
E. V. Safin
Keyword(s):  
Standard Treatment ◽  
Control Group ◽  
Complex Therapy ◽  
Osteopathic Treatment ◽  
Positive Effect ◽  
Experimental Group

Goal of research - the study aims to examine the osteopathic profi le of children with dysarthria and to develop recommendations for osteopathic correction of somatic dysfunctions in 2-3 year old children presenting this pathology.Materials and methods. 30 2-3 year old children with the symptoms of dysarthria took part in the research. All the children were divided into 2 groups: the control group of 15 children received standard treatment, and the experimental group of 15 children received both standard and osteopathic treatment. The dysarthria severity and the osteopathic profi le were evaluated with account of the number of somatic dysfunctions at global, regional, and local levels.Results. The osteopathic correction was shown to have a positive effect on dysarthria severity. The study established a correlation between the dysarthria severity in children and the number of somatic dysfunctions at the local level.Conclusion. The study suggests using osteopathic correction of somatic dysfunctions in the complex therapy of dysarthria in children.

Pragmatic instruction in L2-Russian

2020 ◽  
Vol 4 (1) ◽  
pp. 62-95
Author(s):  
Daniele Artoni ◽  
Valentina Benigni ◽  
Elena Nuzzo
Keyword(s):  
Foreign Language ◽  
Speech Acts ◽  
Control Group ◽  
Written Discourse ◽  
Instructional Treatment ◽  
Completion Task ◽  
Positive Effect ◽  
Experimental Group ◽  
National Corpus ◽  
Pragmatic Instruction

Over the last three decades, a growing number of studies have investigated the effects of instruction on the acquisition of pragmatic features in L2. The bulk of this research has focused mainly on the teaching of English as a second/foreign language. However, instructional pragmatic studies in L2-Russian are lacking. The main purpose of our study is to contribute towards filling this gap by analysing the effects of pragmatic instruction on the acquisition of two speech acts by Italian learners of Russian. Furthermore, we aim to explore whether the Multimodal Russian Corpus (MURCO), a multimedia subcorpus of the Russian National Corpus, can be an effective tool for teaching speech acts in L2-Russian. Our research was composed of one experimental group (n = 18) and one control group (n = 11); each was composed of two intact classes of Italian university students at an intermediate level of L2-Russian, who were pre- and post-tested using a written discourse completion task. The experimental group was subjected to a programme of pragmatic instruction – eight thirty-minute MURCO-based lessons devoted to requests and advice, while the control group was taught according to the standard syllabus, that is, with no pragmatic instruction. The results revealed that the use of the target pragmatic features varied significantly in the experimental group, but not in the control group, thus showing a general positive effect of the instructional treatment based on the MURCO corpus. However, some limitations were identified with regard to the usability of this tool by teachers and learners.

INFLUENCE OF STOCK HOUSE LIGHT ON DAIRY PRODUCTIVITY OF COWS

2020 ◽  
Keyword(s):  
Blood Serum ◽  
Physiological State ◽  
Light Level ◽  
Inorganic Phosphorus ◽  
Control Group ◽  
Hemoglobin Content ◽  
Milk Productivity ◽  
Reproductive Ability ◽  
The Cost ◽  
Experimental Group

The article is devoted to the solution of an urgent problem- influence of different lighting modes on the dairy productivity of cows. 2 groups of cows with 20 heads each were formed. In control group, light in the cowshed was 50-75 Lux for a light period of 7.5 h in January to 16.5 h in June, and in experimental group - 150-200 Lux and 16 h, respectively. It was found that the intensity and duration of illumination affects physiological state, reproductive ability and milk productivity of cows. In the experimental group of cows, compared with the control group, hemoglobin content in blood increased by 4.6% (P < 0.01), red blood cells - by 20.6% (P < 0.05), total protein - by 11.2% (P < 0.001), glucose - by 39.1% (P < 0.05). There was a tendency to increase the total calcium and inorganic phosphorus in blood serum of cows of the experimental group. The level of alkaline phosphatase in blood serum of cows in the control group was 71.5% (P < 0.01) higher than that of cows in the experimental group. Milk yield per 1 cow in the experimental cowshed was 433 kg more than in the control. The cost of 1 kg of milk in the experimental group was 0.94 rubles lower, and the profitability of milk production and sales is 9.42% higher than in the control group. To increase the milk productivity of cows, it is recommended to increase light level in barns for tethered keeping to 150-200 Lux, with the duration of lighting in the winter and transition periods of year up to 16 hours per day.

Reproductive traits and blood biochemical parameters of sows when using a vitamin feed additive “LipoCar”

2020 ◽  
pp. 25-31
Author(s):  
I. A. Pushkaryev ◽  
N. M. Kostomakhin ◽  
S. V. Burtseva ◽  
N. A. Novikov
Keyword(s):  
Blood Serum ◽  
Biochemical Parameters ◽  
Total Duration ◽  
Reproductive Traits ◽  
Feed Additive ◽  
Control Group ◽  
Large White ◽  
Basic Diet ◽  
Blood Biochemical ◽  
Experimental Group

The reproductive traits of sows are of paramount importance in the production of high-quality pork. The world experience of pig farming shows the need to solve first of all the feed problem. Diets unbalanced in the content of vitamins restrain the manifestation of genetically inherent high prolificacy of sows. The purpose of the research was to study the influence of feeding the vitamin feed additive “LipoCar” on the reproductive traits of sows and the biochemical parameters of blood serum. The results of research on the introduction of vitamin feed additive “LipoCar” into the diet of pregnant sows have been presented in the article. The experiment has been carried out in the production conditions in JSC “Linevsky pedigree farm” on sows of Large White breed. Pregnant sows have received a basic diet, balanced for all the normalized elements of nutrition. Sows of the experimental group in the composition of the basic diet fed the feed additive “LipoCar”. The dosage of the drug “Lipocar” was 2,1 g/head/day. “Lipocar” has been fed for 20 days. The total duration of the experiment was 3 months. When introducing the vitamin feed additive “LipoCar” into the main diet of sows in the second half of pregnancy the increase in the number of piglets in the litter by 1,6-6,8 % and the weight of the litter – by 5,6-56,2 % (P ≤ 0,001) have been revealed in comparison with the control group of animals. There was a higher content of carotene in the blood serum of sows of the experimental group by 50,0 % (P ≤ 0,01), vitamin A - by 48,7 % (P ≤ 0,05), and total protein – by 4,9 % (P ≤ 0,05).

scholarly journals UTILIZATION POSSIBILITIES OF PREBIOTICS AND PROBIOTICS IN PREVENTION AND HEALTH CARE OF CALVES

2013 ◽  
Vol 17 (2) ◽  
pp. 121-127
Author(s):  
Luboš Zábranský ◽  
Miloslav Šoch ◽  
Pavel Novák ◽  
Jan Brouček ◽  
Anna Šimková ◽  
...  
Keyword(s):  
Health Care ◽  
Statistical Significance ◽  
Control Group ◽  
Seaweed Extracts ◽  
Weight Gains ◽  
Positive Effect ◽  
Experimental Group

Abstract the aim of our study was to evaluate influence of probiotic agents (Lactovita) and seaweed extracts (Biopolym) with antidiarrheal effects on the prevention of diarrhoea in calves. In the experiment 22 experimental and 22 control calves were observed. The results of the observed incidence of diarrhoea in the control and experimental groups showed a positive effect of probiotic Lactovita and seaweed extracts Biopolymer. 32% of calves in the experimental group and 45% of calves in the control group became sick. Unfortunately, we could not demonstrate the statistical significance of these differences. The weight gains found in the 4th week after birth were by 37.6% higher in the group with Lactovita and Biopolym than in the control group.

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