scholarly journals Chemical and microbiological characterization of Cambisols, Luvisols and Stagnosols

2009 ◽  
Vol 55 (No. 6) ◽  
pp. 231-237 ◽  
Author(s):  
L. Růžek ◽  
M. Růžková ◽  
K. Voříšek ◽  
J. Kubát ◽  
M. Friedlová ◽  
...  
Keyword(s):  
Microbial Biomass Carbon ◽  
Dry Mass ◽  
Soil Samples ◽  
Total Carbon ◽  
Water Addition ◽  
Soil Extracts ◽  
The Czech Republic ◽  
S 100 ◽  
Microwave Sterilization

Cambisols, Luvisols and Stagnosols are main soil taxonomical units covering 78% of the total area of the Czech Republic. In the period 2001–2008 soil samples from 13 arable and 2 grassed localities were collected and tested. Microbial biomass carbon (MBC) was determined using microwave irradiation method (MW), chloroform fumigation extraction method (FE) and re-hydration technique (RHD). Soil samples were moistened, according to the content of organic carbon (C<sub>org</sub>), by deionized water addition (%C<sub>org</sub> × 0.19 ml/g DM – dry mass of soil) immediately before MBC determination. Microwave sterilization (800 J/g DM = 600 W, 2 × 67 s, 100 g DM (10 soil samples) and microwave soil extracts digestion (800 J/ml = 250 W, 77 s, 24 ml) give the lowest values of MBC (204 ± 67 mg/kg DM; 100%) in comparison with FE (236 ± 57 mg/kg DM; 116%) and RHD (478 ± 138 mg/kg DM; 235%), respectively. High significant correlation (<i>r</i> = 0.9713) was found between TC (total carbon; 1.36 ± 0.29%) and TN (total nitrogen; 0.15 ± 0.03%) determined by CNS analyzer. Furthermore, high significant correlations were found between MBC-MW and MBC-RHD (<i>r</i> = 0.8965) as well as MBC-FE and DHA (dehydrogenase activity; <i>r</i> = 0.8094), respectively. DHA in studied soils reached 147 ± 68 mg of triphenylformazan/kg DM/24 h. C<sub>org</sub> formed 96% of TC and total Kjeldahl nitrogen 97% of TN, respectively. According our results MW is fully acceptable for MBC determination.

Characterization of dissolved organic matter (DOM) extracted from soils by hot water percolation (HWP)

2010 ◽  
Vol 59 (1) ◽  
pp. 99-108 ◽  
Author(s):  
M. Takács ◽  
Gy. Füleky
Keyword(s):  
Organic Matter ◽  
Humic Acid ◽  
Natural Organic Matter ◽  
Extraction Methods ◽  
Soil Samples ◽  
Hot Water ◽  
Soil Extracts ◽  
Water Percolation ◽  
Soil Humic Acid

The Hot Water Percolation (HWP) technique for preparing soil extracts has several advantages: it is easily carried out, fast, and several parameters can be measured from the same solution. The object of this study was to examine the possible use of HWP extracts for the characterization of soil organic matter. The HPLC-SEC chromatograms, UV-VIS and fluorescence properties of the HWP extracts were studied and the results were compared with those of the International Humic Substances Society (IHSS) Soil Humic Acid (HA), IHSS Soil Fulvic Acid (FA) and IHSS Suwannee Natural Organic Matter (NOM) standards as well as their HA counterparts isolated by traditional extraction methods from the original soil samples. The DOM of the HWP solution is probably a mixture of organic materials, which have some characteristics similar to the Soil FA fractions and NOM. The HWP extracted organic material can be studied and characterized using simple techniques, like UV-VIS and fluorescence spectroscopy.

scholarly journals Influence of organic and mineral fertilisation on organic matter fractions of a Brazilian Acrisol under maize/common bean intercrop

Soil Research ◽  
2007 ◽  
Vol 45 (1) ◽  
pp. 25 ◽  
Author(s):  
L. F. C. Leite ◽  
E. S. Mendonça ◽  
P. L. O. A. Machado
Keyword(s):  
Organic Carbon ◽  
Common Bean ◽  
Microbial Biomass Carbon ◽  
Soil Classification ◽  
Soil Samples ◽  
Total Carbon ◽  
Carbon Pools ◽  
Organic Matter Fractions ◽  
The Impact ◽  
Organic Compost

In 1984, a field experiment was initiated in Coimbra, State of Minas Gerais, Brazil, involving the combination of 3 levels of mineral fertilisers at control (0); 10 kg N/ha, 15 kg P/ha, and 17 kg K/ha (MF1); 20 kg N/ha, 30 kg P/ha, and 34 kg K/ha (MF2); and 2 levels of organic compost at control (0) and 40 m3/ha (OC) in a maize/common bean intercrop. Soil samples were collected (0–0.10 and 0.10–0.20 m) in 2000 to evaluate the impact of mineral and organic compost on total carbon (TOC) and nitrogen (TN) stocks and on organic carbon pools of a Ferric Acrisol (Chromosol in the Australian Soil Classification). Additional soil samples were collected from an adjacent site covered by secondary Atlantic Forest as a reference. The conversion of forest to agriculture caused a reduction in most of TOC, TN, and microbial biomass carbon, free-light fraction carbon (CLF), and non-labile carbon. The carbon pools in cultivated plot were enhanced by the addition of compost alone. At both depths, TOC and TN stocks were higher (P < 0.05) in the MF2 + OC than MF2 treatment. Compared to soils that have received mineral fertiliser alone or combined with compost, the stocks of labile organic carbon, TN, and CLF were significantly affected (P < 0.05) by the sole application of compost.

scholarly journals Spectroscopic Characteristics of Dissolved Organic Matter in Afforestation Forest Soil of Miyun District, Beijing

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Shi-Jie Gao ◽  
Chen Zhao ◽  
Zong-Hai Shi ◽  
Jun Zhong ◽  
Jian-Guo Liu ◽  
...  
Keyword(s):  
Organic Carbon ◽  
Microbial Biomass Carbon ◽  
Soil Samples ◽  
Hydroxyl Groups ◽  
H Nmr ◽  
Spectroscopic Characteristics ◽  
Aromatic Components ◽  
A Minor ◽  
Aliphatic Chains

In this study, soil samples collected from different plain afforestation time (1 year, 4 years, 10 years, 15 years, and 20 years) in Miyun were characterized, including total organic carbon (TOC), total nitrogen (TN), total phosphorus (TP), available K (K+), microbial biomass carbon (MBC), and dissolved organic carbon (DOC). The DOM in the soil samples with different afforestation time was further characterized via DOC, UV-Visible spectroscopy, excitation-emission matrix (EEM) fluorescence spectroscopy, and1H NMR spectroscopy. The results suggested that the texture of soil sample was sandy. The extracted DOM from soil consisted mainly of aliphatic chains and only a minor aromatic component. It can be included that afforestation can improve the soil quality to some extent, which can be partly reflected from the indexes like TOC, TN, TP, K+, MBC, and DOC. And the characterization of DOM implied that UV humic-like substances were the major fluorophores components in the DOM of the soil samples, which consisted of aliphatic chains and aromatic components with carbonyl, carboxyl, and hydroxyl groups.

PENGEMBANGAN TEKNOLOGI SELEKSI IN VITRO BERULANG (REPEAT CYCLING–IN VITRO SELECTION) TOLERAN STRES KEKERINGAN UNTUK PERBAIKAN MUTU GENETIK DAN PRODUKSI TANAMAN KEDELAI BERMIKORIZA

2017 ◽  
Vol 1 (1) ◽  
pp. 74-84
Author(s):  
Ahmad Riduan ◽  
Rainiyati Rainiyati ◽  
Yulia Alia
Keyword(s):  
Arbuscular Mycorrhizae ◽  
In Vitro Selection ◽  
Soil Samples ◽  
Single Spore ◽  
Isolation And Characterization ◽  
Single Spore Culture ◽  
Spore Culture ◽  
Glomus Sp

Every plant rhizospheres in any ecosystem there are various living microorganisms including Arbuscular Mycorrhizae Fungi (AMF).  An isolation and characterization is required to investigate the species or type of the AMF. This research was aimed at studying the isolation and characterization of AMF sporulation in soybean rhizospheres in Jambi Province. The results of evaluation on soil samples before trapping showed that there are spores from three genus of AMF twelve types Glomus , two types Acaulospora and one type of Enthrophospora.  Following single spore culture in soybean rhizosphere, 5 spore types were obtained:  Glomus sp-1, Glomus sp-4, Glomus sp-7, Glomus sp-8 Glomus sp-10.

scholarly journals Screening and Characterization of Potent Poly Glutamic Acid Producing Bacillus sp. Isolated from Kinema, Water and Soil Samples

Heliyon ◽  
2021 ◽  
pp. e07715
Author(s):  
Punam Thapa ◽  
Alina Thapa ◽  
Sujan Khadka ◽  
Sanjeep Sapkota ◽  
Om Prakash Panta ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Soil Samples ◽  
Bacillus Sp ◽  
Water And Soil Samples

Production and characterization of mouse monoclonal antibodies against S-100 protein

1985 ◽  
Vol 10 (2) ◽  
pp. 179
Author(s):  
G.Y. Gillespie ◽  
P. Golden ◽  
J.M. Bynum ◽  
M.S. Mahaley
Keyword(s):  
Monoclonal Antibodies ◽  
S 100

Geochemical characterization of soil samples from gold mining areas using PIXE spectroscopy

2018 ◽  
Vol 26 (5) ◽  
pp. 4924-4937 ◽  
Author(s):  
Felix Samuel Olise ◽  
Samuel Adedigba Adeojo ◽  
Oyediran Kayode Owoade ◽  
Oyebamiji Oyedele Oketayo ◽  
Solomon Adeniyi Adekola ◽  
...  
Keyword(s):  
Gold Mining ◽  
Soil Samples ◽  
Geochemical Characterization ◽  
Mining Areas

scholarly journals ISOLATION AND CHARACTERIZATION OF ACTINOMYCETES FROM SOIL OF AD-DAWADMI, SAUDI ARABIA AND SCREENING THEIR ANTIBACTERIAL ACTIVITIES

2017 ◽  
Vol 9 (10) ◽  
pp. 276 ◽  
Author(s):  
Gouse Basha Sheik ◽  
Muazzam Sheriff Maqbul ◽  
Gokul Shankar S. ◽  
Ranjith M S
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Activities ◽  
Soil Samples ◽  
Streptomyces Sp ◽  
Crude Extracts ◽  
Test Organisms ◽  
Inhibition Zones

Objective: To isolate and characterize novel actinomycetes and to evaluate their antibacterial activity against drug-resistant pathogenic bacteriaMethods: In the present study, 19 soil samples were collected from different localities of Ad-Dawadmi, Saudi Arabia. Actinomycetes were isolated from these samples using serial dilution and plating method on Actinomycetes isolation agar supplemented with nalidixic acid and actidione to inhibit bacteria and fungi. Crude extracts of potential actinomycetes were produced by submerged fermentation. The antimicrobial activity of crude extracts of actinomycetes was tested against different bacteria using the agar well diffusion method. Characterization of the isolates was done by morphological, physiological and biochemical methods.Results: A total of 9 (47%) isolates of actinomycetes were isolated from 19 different soil samples tested. Among them, 4 (44%) isolates confirmed as Streptomyces sp. showed potential antimicrobial activity against one or more test organisms. Crude extracts were made from these 4 actinomycetes isolates(DOM1, DOM3, DP3, DP4)and tested for their antibacterial activities against 4 different clinical bacterial strains (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus). Crude extract from DP3 isolate showed highest antibacterial activity against all the four test organisms (28 mm, 21 mm, 20 mm and 18 mm) respectively and DP4 showed lowest antibacterial activity against all the four test organisms (14 mm, 12 mm, 0 mm, 6 mm) respectively. The highest zone of inhibition was shown by DP3 against Staphylococcus aureus (28 mm) and Escherichia coli was resistant for DP4. Most of the Inhibition zones produced by crude extracts showed significant differences when compared with control, tested against test organisms (P<0.05). Inhibition zones produced by DP3 and DOM1 against Staphylococcus aureus were 28 mm and 23 mm, respectively which were strong active when compared with control Ciprofloxacin (18 mm).Conclusion: Further studies for purification of bioactive metabolites and molecular characterization analysis of isolated Streptomyces sp. are in progress which would be helpful in discovering novel compounds of commercial value.

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