scholarly journals The effect of humic acid on mercury accumulation in chicken organs and muscle tissues

2008 ◽  
Vol 53 (No. 11) ◽  
pp. 472-478 ◽  
Author(s):  
Z. Zralý ◽  
B. Písaříková ◽  
M. Navrátilová
Keyword(s):  
Humic Acid ◽  
Methyl Mercury ◽  
Day Treatment ◽  
Basal Diet ◽  
Control Group ◽  
Mercury Accumulation ◽  
Concurrent Treatment ◽  
Tissue Samples ◽  
Muscle Tissues ◽  
Experimental Group

Forty female chickens were allocated to four groups of ten birds each. The control group (K) was fed a basal diet without supplementation. The second control group received a basal diet with humic acid (HA) at a dose of 0.5 g per chicken/day. The first experimental group was fed the basal diet with methyl-mercury (MeHg) at a dose of 0.02 mg per chicken/day and the group of chickens MeHg + HA received 0.5 g HA per chicken/day. After slaughter, mercury levels were assessed in liver, kidney, brain and muscle tissue samples. After 10-day treatment of the chickens with MeHg, significantly increased (<I>P</I> < 0.001) Hg concentrations were detected in all examined tissues in comparison with the groups K and HA. The average concentrations in liver, kidneys, brain and muscle tissues were 424, 398, 81.6 and 79.2 µg Hg/kg, respectively. After concurrent treatment with HA and MeHg, Hg concentrations were lower by 20.6%, 23.8%, 23.0% and 18.6% in liver, kidneys, brain and muscle tissues (<I>P</I> < 0.001). Biological accumulation of Hg was 25.5% and 20.4% in MeHg and MeHg + HA groups, respectively.

scholarly journals Effects of Dietary Inclusion of Bilberry and Walnut Leaves Powder on the Digestive Performances and Health of Tetra SL Laying Hens

Animals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 823 ◽  
Author(s):  
Roua Gabriela Popescu ◽  
Sorina Nicoleta Voicu ◽  
Gratiela Gradisteanu Pircalabioru ◽  
Alina Ciceu ◽  
Sami Gharbia ◽  
...  
Keyword(s):  
Histological Analysis ◽  
Specific Activity ◽  
Basal Diet ◽  
Intestinal Content ◽  
Final Concentration ◽  
Control Group ◽  
Villus Height ◽  
Tissue Samples ◽  
Walnut Leaves ◽  
Digestive Health

The purpose of this study was to examine the effects of dietary inclusion of two additives at the final concentration of 0.5% bilberry (E1) and 1% walnut (E2) leaves powder in the basal diet on digestive health of hens. A total number of 90 Tetra SL hens were divided into two experimental groups (E1 and E2) and one control group (C) consisting of 30 hens each. After four weeks, 10 hens of each group were sacrificed and tissue samples and intestinal content were taken from the duodenum, jejunum, and cecum in order to perform histological, enzymatic, and microbiota analyses. In groups E1 and E2, the histological analysis showed a significant increase of villus height, resulting probably in increased absorption of nutrients in duodenum and jejunum. A decrease in the specific activity of alpha-amylase and trypsin in E1 and E2 for both duodenum and jejunum compared to the control one was also recorded. In addition, the maltase and invertase specific activity in duodenum increased, a tendency that was kept for maltase but not for invertase in jejunum. The cecal microbiota of E1 and E2 individuals was characterized by an increase of Firmicutes and Lactobacilli and a decrease of Enterobacteriaceae. In conclusion, our results indicate that bilberry and walnut leaves additives in feed may improve the health status of the poultry gastrointestinal tract.

Analiza wpływu stosowania kremu z pszczelim pyłkiem kwiatowym na wybrane cechy skóry rąk oceniane subiektywnie i obiektywnie

2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Anna Piotrowska ◽  
Magdalena Zając ◽  
Łukasz Tota ◽  
Olga Czerwińska ◽  
Natalia Totko-Borkusiewicz ◽  
...  
Keyword(s):  
Day Treatment ◽  
Subjective Evaluation ◽  
Biologically Active ◽  
Skin Hydration ◽  
Control Group ◽  
Skin Tightening ◽  
Bee Pollen ◽  
Allergy Test ◽  
Before And After ◽  
Experimental Group

Introduction. Pollen is a plant-based product and is a rich source of biologically active compounds such as: proteins, amino acids, carbohydrates, fats and fatty acids, phenolic compounds and enzymes, considered as an promising ingredient in cosmetic formulations. Aim. The aim of this study was to analyze the effect of a cream enriched with micronized bee pollen on selected skin characteristics evaluated in subjective and objective manner. Material and methods. The participants were 13 female volunteers (students) who met the following criteria: no dermatological and endocrine diseases, non-smoking and no special or elimination diets up to 3 months before the study, and also a negative allergy test result. Students were randomly assigned to the following groups: the experimental group – subjects using cream with 3% micronized bee pollen and the control one using cream base. Before and after the 5-day treatment, the participants undewent the measurements of skin hydration. The subjective evaluation of selected skin properties was examined by using a 3-grade scale. Results. All skin characteristics evaluated subjectively improved as a result of pollen-enriched formula treatment. The participants of the experimental group have noticed a significant change in the following properties: moisturizing, smoothness and skin tightening. The final measurement of skin hydration has showed an increase of 12.14 units in the experimental group and 4.52 units in the control group, indicating difference between groups (p = 0.0389). Also a positive correlation between application time and moisturizing (R = 0.929) has been found. Conclusions. The micronized bee pollen, as an active ingredient in protective and moisturizing preparations, seems to be an effective ingredient.

scholarly journals Healing of abdominal wall aponeurosis of rats after incision with either cold scalpel or electrocautery

2007 ◽  
Vol 22 (suppl 1) ◽  
pp. 12-15 ◽  
Author(s):  
Ademar Garcia ◽  
José Eduardo Aguilar-Nascimento ◽  
Eduardo Mulinari Darold ◽  
Rodolfo Edson de Franco Pimentel ◽  
Eloísa Almeida Curvo ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Histological Study ◽  
Control Group ◽  
Hydroxyproline Content ◽  
Tissue Samples ◽  
Significant Difference ◽  
Experimental Group

PURPOSE: To compare the healing of abdominal wall aponeurosis of rats after incision with either cold scalpel or electrocautery. METHODS: Twelve male adults Wistar inbreed rats weightin 258-362g entered the experiment. They were randomly divided in two groups, control group (N= 6) and experimental group (N= 6). All animals underwent anesthesia followed abdominal wall incision with either cold scalpel (controls) or electrocautery (experimental group). The animals were killed on the 10th day and necropsied. Tissue samples containig the abdominal wall aponeurosis were sent for both histological study and hydroxyproline content assay. RESULTS: The histological study showed no significant difference between the two groups. Hydroxiproline content in experimenral group (128,56 [78,98-198,92] µg /100m) was not significantly different from control group (140,27 [116,20-166,59] mg /100ml; p = 0.53). CONCLUSION: Healing of abdominal wall after dieresis with either cold scalpel or electrocautery are equivalent and do not differ.

Prevention of Capsular Contracture with Montelukast After Insertion of Silicone Implants in Rabbits

2016 ◽  
Vol 13 (10) ◽  
pp. 7623-7627
Author(s):  
Zhenyu Jin ◽  
Ki Yong Hong ◽  
Kyung Won Minn ◽  
Hak Chang ◽  
Ung Sik Jin
Keyword(s):  
Breast Implant ◽  
Capsular Contracture ◽  
White Female ◽  
Silicone Implants ◽  
Control Group ◽  
Tissue Samples ◽  
Significant Difference ◽  
Group A ◽  
The Right ◽  
Experimental Group

Capsular contracture is the most common complication after insertion of silicone implants during breast implant surgery. The discovery that myofibroblasts play an important role in the formation of hypertrophic scars led to the development of pharmacological drugs such as zafirlukast, which prevents capsular contracture by resisting the above mechanism. As a result, the author sought to investigate the effect of the anti-leukotriene montelukast on capsular contracture. Ten white female New Zealand rabbits, each weighing approximately 3 kg, were used as subjects. Through bilateral incision of the midback area, the prostheses were inserted on the subpanniculus carnosus plane. Once the silicone prostheses had been inserted, the right implant was injected with 10 mL of montelukast (10 µg/mL), and the left implant was injected with 10 mL of normal saline. Eight weeks after the procedure, the capsular pressure was measured via tonometry using a circular glass piece weighing 42.7 g. The tissue samples were then extracted, and their thicknesses were measured using hematoxylin-eosin stain and Masson trichrome stain. The average pressure was 4.23±0.99 mmHg in the control group and 3.71±0.51 mmHg in the experimental group, a statistically significant difference (p = 0.02). The average capsular thickness was 947.938±300 µm in the control group and 709.672±274 µm in the experimental group, a statistically significant difference (p = 0.028). The author confirmed that montelukast injections during silicone prosthesis insertion decreased the formation of capsular contracture.

scholarly journals The impact of humic acid additives on mineral metabolism of rabbits in the postnatal period of ontogenesis

2020 ◽  
Vol 11 (2) ◽  
pp. 289-293
Author(s):  
M. A. Rybalka ◽  
L. M. Stepchenko ◽  
O. O. Shuleshko ◽  
L. V. Zhorina
Keyword(s):  
Alkaline Phosphatase ◽  
Humic Acid ◽  
Blood Serum ◽  
Bone Tissue ◽  
Mineral Metabolism ◽  
Histological Study ◽  
Postnatal Period ◽  
Control Group ◽  
Copper And Zinc ◽  
Experimental Group

Humic acids are characterized by chelating properties due to which they are able to influence the mineral metabolism in animals. Rabbits have species-specific characteristics of mineral metabolism, which distinguishes them from most species of mammals. For the experiment, 16 rabbits of 45-day-old of Hyplus breed were divided into the experimental and control group of 8 rabbits in each. The animals were 15 days in the preparatory period. The rabbits of the experimental group received humic acid additive individually with water in the amount of 5 mg/kg, the rabbits of the control group received pure water in the same amount. The ability of humic acid additive to increase the content of calcium, ionized calcium, iron, to correct the content of copper and zinc, and also to increase the activity of alkaline phosphatase in the blood serum of rabbits of the experimental group has been proved. We observed the early effect of the humic acid additive on increasing the content of iron and calcium ionized and a later effect on the activity of alkaline phosphatase and an increase in the content of copper in the blood serum. The influence of humic acid additive on the distribution of calcium, phosphorus, manganese, copper and zinc in bone tissue is determined. According to a histological study, there was a significant increase in the number of layers of osteons and osteoblasts in the bone tissue of the femur, an increase in the number of osteons and osteoblasts in the bone tissue of the sternum and an increase in the number of columns of chondrocytes and an increase in the number of chondrocytes in the column in the cartilaginous tissue of the sternum. The results of a histological study of bone tissue, together with an increase in structural macroelements in it and redistribution of osteotropic trace elements in the blood and bone tissue suggest the possibility of using a humic acid additive to intensify the growth and mineralization of bone tissue, which can improve the state of bone tissue of rabbits in the postnatal period ontogenesis.

scholarly journals Effect of probiotics and Yucca schidigera extract supplementation on broiler meat quality

2020 ◽  
Vol 42 ◽  
pp. e48066
Author(s):  
Karima Benamirouche ◽  
Djamila Baazize-Ammi ◽  
Nadia Hezil ◽  
Reda Djezzar ◽  
Abdellatif Niar ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Meat Quality ◽  
Ph Value ◽  
Saturated Fatty Acids ◽  
Basal Diet ◽  
Control Group ◽  
Broiler Meat ◽  
Yucca Schidigera ◽  
Thigh Muscles ◽  
Experimental Group

The current study investigated the effect of dietary supplementation with probiotics and Yucca schidigera extract on physicochemical parameters, proximate composition, mineral content and fatty acid profile of broiler breast and thigh muscles. In total, 240 one-day old broilers were randomly allocated into two dietary treatments groups: 1) Control (basal diet), 2) experimental (basal diet with two probiotics Pediococcus acidilactici and Saccharomyces cerevisiae and Yucca schidigera extract). The results showed that the pH value was higher in the experimental group than in the control group (p < 0.05). However, drip, cook and thaw losses were not influenced by dietary treatment (p > 0.05). A significant increase in protein, Fe, Zn, Na, P and a significant decrease in lipid, Cu and Cr contents was exhibited in experimental group relative to control group (p < 0.05). The proportion of stearic acid and saturated fatty acids was significantly (p < 0.05) reduced, whereas linoleic acid and polyunsaturated fatty acids contents were significantly (p < 0.05) increased in breast and thigh muscles of fed the experimental diet. We concluded that additive supplementation of the diet with probiotics and Yucca schidigera extract could improve meat quality.

scholarly journals The Effectiveness of Dietary Byproduct Antioxidants on Induced CYP Genes Expression and Histological Alteration in Piglets Liver and Kidney Fed with Aflatoxin B1 and Ochratoxin A

Toxins ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 148
Author(s):  
Roua Gabriela Popescu ◽  
Cristina Bulgaru ◽  
Arabela Untea ◽  
Mihaela Vlassa ◽  
Miuta Filip ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ochratoxin A ◽  
Aflatoxin B1 ◽  
Basal Diet ◽  
Sea Buckthorn ◽  
Expression Level ◽  
Control Group ◽  
Tissue Samples ◽  
Perform Gene Expression ◽  
Liver And Kidney

The purpose of this study was to investigate the potential of a byproduct mixture derived from grapeseed and sea buckthorn oil industry to mitigate the harmful damage produced by ochratoxin A and aflatoxin B1 at hepatic and renal level in piglets after weaning. Forty cross-bred TOPIGS-40 hybrid piglets after weaning were assigned to three experimental groups (E1, E2, E3) and one control group (C), and fed with experimental diets for 30 days. The basal diet was served as a control and contained normal compound feed for starter piglets without mycotoxins. The experimental groups were fed as follows: E1—basal diet plus a mixture (1:1) of two byproducts (grapeseed and sea buckthorn meal); E2—the basal diet experimentally contaminated with mycotoxins (479 ppb OTA and 62ppb AFB1); and E3—basal diet containing 5% of the mixture (1:1) of grapeseed and sea buckthorn meal and contaminated with the mix of OTA and AFB1. After 4 weeks, the animals were slaughtered, and tissue samples were taken from liver and kidney in order to perform gene expression and histological analysis. The gene expression analysis showed that when weaned piglets were fed with contaminated diet, the expression of most analyzed genes was downregulated. Among the CYP450 family, CYP1A2 was the gene with the highest downregulation. According to these results, in liver, we found that mycotoxins induced histomorphological alterations in liver and kidney and had an effect on the expression level of CYP1A2, CYP2A19, CYP2E1, and CYP3A29, but we did not detect important changes in the expression level of CY4A24, MRP2 and GSTA1 genes.

scholarly journals Absorption of iodotyrosine from iodized milk protein in animals

2020 ◽  
Vol 8 (1) ◽  
pp. 60-66
Author(s):  
Larisa Bolshakova ◽  
Dmitry Lukin
Keyword(s):  
Blood Plasma ◽  
Muscle Tissue ◽  
Potassium Iodide ◽  
Milk Protein ◽  
Control Group ◽  
Animal Group ◽  
Rat Blood ◽  
Organic Iodine ◽  
Muscle Tissues ◽  
Experimental Group

Introduction. One of the ways to the solve iodine deficiency problem is the addition of iodine to farm animal feed. It allows producing iodized livestock products. Promising sources of organic iodine are iodotyrosine-containing iodized milk proteins. Organic iodine accumulation in organs and tissues has not been sufficiently studied. Study objects and methods. We determined iodotyrosine content in rat blood plasma and in pig muscle tissue. For this purpose, high performance liquid chromatography with mass spectrometric detection and cathodic stripping voltammetry were used. Results and discussion. At the first stage of the study, we examined iodotyrosines in rat blood plasma after a single administration of iodized milk protein or potassium iodide (30 μg I/kg weight) at specific time intervals. A significant increase in the concentration of monoiodotyrosine and diiodotyrosine was recorded 4 and 24 h after the administration. At the second stage, we studied the accumulation of iodotyrosines in the muscle tissue of pigs during their fattening period (104 days). The diet of the control animal group included potassium iodide (0.6 mg I/kg of feed). The experimental groups A and B got iodized milk protein (0.3 and 0.6 mg I/kg of feed, respectively). Monoiodotyrosin content in the muscle tissue of pigs of the experimental groups was 3.0 and 5.2 times higher than that in the control group. Diiodotyrosine content was 4.9 and 8.2 times higher. In the experimental group A, iodine content in muscle tissues was 26% higher than that in the control group, in the experimental group B it was 72% higher. Calculations of iodine intake balance and its accumulation in muscle tissues showed that in animals whose diet included iodized milk protein, the iodine assimilation was much higher (0.70 and 0.53%) than in the control group (0.21%). Conclusion. Iodotyrosines from iodized milk protein are absorbed by the gastrointestinal tract in an unchanged form and accumulate in muscle tissues. The findings give more clear understanding of physiological and biochemical mechanisms of organic iodine absorption in animals.

scholarly journals The impact of humic acid additives on mineral metabolism of rabbits in the postnatal period of ontogenesis

2020 ◽  
Vol 11 (2) ◽  
pp. 289-293
Author(s):  
M. A. Rybalka ◽  
L. M. Stepchenko ◽  
O. O. Shuleshko ◽  
L. V. Zhorina
Keyword(s):  
Alkaline Phosphatase ◽  
Humic Acid ◽  
Blood Serum ◽  
Bone Tissue ◽  
Mineral Metabolism ◽  
Histological Study ◽  
Postnatal Period ◽  
Control Group ◽  
Copper And Zinc ◽  
Experimental Group

Humic acids are characterized by chelating properties due to which they are able to influence the mineral metabolism in animals. Rabbits have species-specific characteristics of mineral metabolism, which distinguishes them from most species of mammals. For the experiment, 16 rabbits of 45-day-old of Hyplus breed were divided into the experimental and control group of 8 rabbits in each. The animals were 15 days in the preparatory period. The rabbits of the experimental group received humic acid additive individually with water in the amount of 5 mg/kg, the rabbits of the control group received pure water in the same amount. The ability of humic acid additive to increase the content of calcium, ionized calcium, iron, to correct the content of copper and zinc, and also to increase the activity of alkaline phosphatase in the blood serum of rabbits of the experimental group has been proved. We observed the early effect of the humic acid additive on increasing the content of iron and calcium ionized and a later effect on the activity of alkaline phosphatase and an increase in the content of copper in the blood serum. The influence of humic acid additive on the distribution of calcium, phosphorus, manganese, copper and zinc in bone tissue is determined. According to a histological study, there was a significant increase in the number of layers of osteons and osteoblasts in the bone tissue of the femur, an increase in the number of osteons and osteoblasts in the bone tissue of the sternum and an increase in the number of columns of chondrocytes and an increase in the number of chondrocytes in the column in the cartilaginous tissue of the sternum. The results of a histological study of bone tissue, together with an increase in structural macroelements in it and redistribution of osteotropic trace elements in the blood and bone tissue suggest the possibility of using a humic acid additive to intensify the growth and mineralization of bone tissue, which can improve the state of bone tissue of rabbits in the postnatal period ontogenesis.

scholarly journals Effects of Dietary Tea Polyphenols on Epigallocatechin Gallate, Catechin, Egg Quality and Production of Gallus domestiaus

2021 ◽  
Vol 25 (01) ◽  
pp. 139-145
Author(s):  
Zhenzhen Fan
Keyword(s):  
Egg Production ◽  
Egg Quality ◽  
Epigallocatechin Gallate ◽  
Basal Diet ◽  
Egg Yolk ◽  
Control Group ◽  
Egg Weight ◽  
Quality Testing ◽  
High Concentrations ◽  
Experimental Group

Effect of dietary tea polyphenol (TP) on the content of epigallocatechin gallate (EGCG), catechin (C) in egg yolk, and impact on egg quality and production was investigated. For this purpose, 315 healthy Gallus domestiaus aged 42 weeks were selected and randomly divided into 7 groups. The experimental group added 0.02, 0.05, 0.09, 0.14, 0.19 and 0.24% TP to the basic diet. The control group (CK) was fed basal diet. After 10, 20 and 30 days, 15 eggs were randomly selected from each group for egg quality testing, and 20 eggs were selected for EGCG and C content test in the yolk. The results showed that in the 0.24% TP group, the content of EGCG in egg yolk was significantly higher than that of CK group and the 0.02, 0.05, 0.09 and 0.14% TP groups (P < 0.05), and reached the highest on the 30th day (19.171μg/g). The content of C in egg yolk was significantly higher than that in CK group and the 0.02, 0.05, 0.09 and 0.14% TP groups (P < 0.05); and reached the highest on 30th day (7.071 μg/g). The egg weight of the 0.14% TP group on the 10th day and of the 0.19% TP group on the 20th day were significantly lower than that of the CK group (P < 0.05); the egg yolk weight of 0.14% TP on the 10th and 20th days were significantly lower than that of the CK group (P < 0.05). On the 30th day, compared with the control group, the egg weight of the 0.14% TP group was significantly reduced (P < 0.05), the egg yolk color of 0.05 and 0.14% TP groups was significantly increased (P < 0.05). During the whole experiment, the egg production of 0.24% TP group was significantly higher than that of control group (P < 0.05). In conclusion, adding high concentrations of TP can increase the content of EGCG and C in the yolk; however, it has a certain impact on egg weight, yolk color and eggshell thickness. Adding high concentrations of TP may increase egg production. © 2021 Friends Science Publishers

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