scholarly journals Characterization and expression of high temperature stress responsive genes in bread wheat (Triticum aestivum L.)

2011 ◽  
Vol 47 (Special Issue) ◽  
pp. S94-S97 ◽  
Author(s):  
P. Khurana ◽  
H. Chauhan ◽  
N. Khurana
Keyword(s):  
Triticum Aestivum ◽  
Heat Stress ◽  
High Temperature ◽  
Temperature Stress ◽  
Abiotic Stresses ◽  
Developmental Stages ◽  
High Temperature Stress ◽  
Homology Search ◽  
Cdna Libraries ◽  
Pcr Analysis

To elucidate the effects of high temperatures, wheat plants (Triticum aestivum cv. CPAN 1676) were given heat shocks at 37°C and 42°C for two hours, and responsive genes were identified through PCR-Select Subtraction technology. Four subtractive cDNA libraries, including three forward and one reverse subtraction, were constructed from three different developmental stages. A total of 5500 ESTs were generated and 3516 high quality ESTs were submitted to Genbank. More than one third of the ESTs generated fall in unknown/no hit categories upon a homology search through BLAST analysis. A large number of high temperature responsive genes have been identified and characterized. Reverse subtraction analysis in developing grains showed extensive transcriptional changes upon heat stress as revealed by comparative analysis with forward subtraction. Differential expression was confirmed by cDNA macroarray and by northern/RT-PCR analysis. Expression analysis of wheat plants subjected to high temperature stress, after one and four days of recovery, showed fast recovery in seedling tissues. However, recovery was small in the developing seed tissue after two hours of heat stress. Ten selected genes were analysed in further detail by quantitative real-time PCR in an array of 35 different wheat tissues representing major developmental stages as well as different abiotic stresses. Tissue specificity was examined along with cross talk with other abiotic stresses and putative signalling molecules. The results obtained contribute towards understanding the regulation of genes at different developmental stages in wheat crucial to withstanding and recovery from heat stress.

High Temperature Stress in Wild Wheats and Spring Wheats

1994 ◽  
Vol 21 (6) ◽  
pp. 705 ◽  
Author(s):  
JG Waines
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Temperature Stress ◽  
Heat Tolerance ◽  
Developmental Stages ◽  
Diploid Species ◽  
Geographic Area ◽  
High Temperature Stress ◽  
Wild Wheats ◽  
Wild Accessions

The effect of high temperature stress on wild and spring wheats is reviewed. Wild wheats include species in the genera Aegilops L. and Triticum L. Species exist in a polyploid series, diploid, tetraploid and hexaploid, based on the genome formula, n = x = 7 chromosomes. Commercial durum wheat is tetraploid with the genome formula BBAA, while bread wheat is hexaploid (BBAADD). Wheats grown at Riverside, California, from June to October exhibit heat stress at the vegetative and reproductive stages. Under high temperatures (28/15�C day/night) during the vegetative stage, many diploid species do not grow well. Wild diploid T. urartu (AA) and T. monococcum ssp. boeoticum (AA) exhibited more effects of heat stress than the goat grasses A. speltoides (SS = BB?) or A. tauschii (DD). Wild tetraploid T. turgidum L. ssp. dicoccoides Korn (BBAA) exhibited more vegetative-phase stress tolerance than the diploid wheats. Modern Mexican cultivars of durum and bread wheats showed good establishment under high field temperatures, but often tiller number was reduced, and the developmental stages were reduced in time. All the spring durum and bread wheats tested flowered and set seed. They produced anthers with fertile pollen, and they had reproductive heat tolerance. Many wild Aegilops and Triticum accessions did not boot for lack of vernalisation, or they showed reproductive heat stress. Ten wild accessions, including A. speltoides, A. longissima and A. searsii, showed normal vegetative and reproductive development and were considered heat tolerant. They came from the same geographic area in Palestine which should be searched for landraces of wheats that show heat tolerance.

Assessment of wheat (Triticum aestivum L.) genotypes for high temperature stress tolerance using physico-chemical analysis

2020 ◽  
Vol 53 (2) ◽  
Author(s):  
Khalil Ahmed Laghari ◽  
Abdul Jabbar Pirzada ◽  
Mahboob Ali Sial ◽  
Muhammad Athar Khan ◽  
Jamal Uddin Mangi
Keyword(s):  
Triticum Aestivum ◽  
High Temperature ◽  
Chemical Analysis ◽  
Stress Tolerance ◽  
Temperature Stress ◽  
Triticum Aestivum L ◽  
High Temperature Stress ◽  
Physico Chemical

scholarly journals Wheat (Triticum aestivum L.) TaHMW1D Transcript Variants Are Highly Expressed in Response to Heat Stress and in Grains Located in Distal Part of the Spike

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 687
Author(s):  
Chan Seop Ko ◽  
Jin-Baek Kim ◽  
Min Jeong Hong ◽  
Yong Weon Seo
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Temperature Stress ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Grain Filling ◽  
High Temperature Stress ◽  
Two Dimensional Gel Electrophoresis ◽  
Transcript Variants

High-temperature stress during the grain filling stage has a deleterious effect on grain yield and end-use quality. Plants undergo various transcriptional events of protein complexity as defensive responses to various stressors. The “Keumgang” wheat cultivar was subjected to high-temperature stress for 6 and 10 days beginning 9 days after anthesis, then two-dimensional gel electrophoresis (2DE) and peptide analyses were performed. Spots showing decreased contents in stressed plants were shown to have strong similarities with a high-molecular glutenin gene, TraesCS1D02G317301 (TaHMW1D). QRT-PCR results confirmed that TaHMW1D was expressed in its full form and in the form of four different transcript variants. These events always occurred between repetitive regions at specific deletion sites (5′-CAA (Glutamine) GG/TG (Glycine) or (Valine)-3′, 5′-GGG (Glycine) CAA (Glutamine) -3′) in an exonic region. Heat stress led to a significant increase in the expression of the transcript variants. This was most evident in the distal parts of the spike. Considering the importance of high-molecular weight glutenin subunits of seed storage proteins, stressed plants might choose shorter polypeptides while retaining glutenin function, thus maintaining the expression of glutenin motifs and conserved sites.

Emergence of seedlings from different depths following high temperature stress

1975 ◽  
Vol 84 (3) ◽  
pp. 525-528 ◽  
Author(s):  
I. C. Onwueme ◽  
S. A. Adegoroye
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Temperature Stress ◽  
Seedling Emergence ◽  
High Temperature Stress ◽  
Moist Soil ◽  
Different Depths

SUMMARYSeeds of Amaranthus, melon, cowpea and tomato were planted in moist soil at 1, 4 or 7·5 cm depth and subjected to a heat stress of 45 °C for 10 h on the day of sowing (day 0), 1 day after sowing or 2 days after sowing. Seedling emergence was retarded by heat stress, the most drastic retardation being due to heat stress on day 1 for cowpea and tomato, day 2 for melon, and day 0 for Amaranthus. Emergence also decreased with increasing depth of sowing. The interaction of depth and heat stress was also significant in all cases, such that the delay in emergence due to heat stress tended to be greater with increasing depth of sowing. The agronomic significance of the results is discussed.

scholarly journals Differential modulation of heat inducible genes across diverse genotypes and molecular cloning of a sHSP from Pearl millet [Pennisetum glaucum (L.) R. Br.]

2020 ◽  
Author(s):  
S MukeshSankar ◽  
C. Tara Satyavathi ◽  
Sharmistha Barthakur ◽  
S.P Singh ◽  
Roshan Kumar ◽  
...  
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Pearl Millet ◽  
Temperature Stress ◽  
Expression Profiling ◽  
Stability Index ◽  
High Temperature Stress ◽  
Seedling Stage ◽  
Transcript Expression ◽  
Membrane Stability Index

AbstractEnvironmental stresses negatively influence survival, biomass and grain yield of most crops. Towards functionally clarifying the role of heat responsive genes in Pearl millet under high temperature stress, the present study were carried out using semi quantitative RT- PCR for transcript expression profiling of hsf and hsps in 8 different inbred lines at seedling stage, which was earlier identified as thermo tolerant/susceptible lines through initial screening for thermo tolerance using membrane stability index among 38 elite genotypes. Transcript expression pattern suggested existence of differential response among different genotypes in response to heat stress in the form of accumulation of heat shock responsive gene transcripts. Genotypes WGI 126, TT-1 and MS 841B responded positively towards high temperature stress for transcript accumulation for both Pgcp 70 and Pghsf and also had better growth under heat stress, whereas PPMI 69 showed the least responsiveness to transcript induction supporting the membrane stability index data for scoring thermotolerance, suggesting the efficacy of transcript expression profiling as a molecular based screening technique for identification of thermotolerant genes and genotypes at particular crop growth stages. As to demonstrate this, a full length cDNA of Pghsp 16.97 was cloned from the thermotolerant cultivar, WGI 126 and characterized for thermotolerance. The results of demonstration set forth the transcript profiling for heat tolerant genes can be a very useful technique for high throughput screening of tolerant genotypes at molecular level from large cultivar collections at seedling stage.

scholarly journals Phenotypic diversity and marker-trait association studies under heat stress in tomato (Solanum lycopersicum L.)

2019 ◽  
Vol 13 ((04) 2019) ◽  
pp. 578-587 ◽  
Author(s):  
Muhammed Alsamir ◽  
Nabil Ahmad ◽  
Vivi Arief ◽  
Tariq Mahmood ◽  
Richard Trethowan
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Temperature Stress ◽  
Phenotypic Diversity ◽  
Association Studies ◽  
High Temperature Stress ◽  
Control Treatment ◽  
Genome Wide Association Studies ◽  
Phenotypic Data ◽  
Tomato Genotypes

Tomato is a mild season crop and high temperature stress impacts productivity negatively. However, the development of cultivars with improved heat tolerance is possible as genetic variability has been consistently reported. This study aimed to identify candidate genes that impact various traits under heat stress. Genome-wide association studies (GWAS) were conducted on a diverse set of 144 tomato genotypes collected from various germplasm centers and breeding programs. The genotypes were grown under control and heat stress in poly tunnels having mean temperatures of 30°C and 45°C for two seasons and phenotypic data were collected on seven agro-physiological traits. All individuals were genotyped withthe80K DArTseq platform using 31237 SNP markers. Data were analysed using a mixed model based on restricted maximum likelihood (REML). Pattern analysis of the phenotypic data showed five primary clusters each with genotypes from multiple origins. Based on the genotypic data, three wild tomato genotypes showed a degree of un-relatedness with the other materials as they were distantly located from the rest of the genotypes in the scatter plot. Control treatment data were used to ascertain markers that are exclusively important under high temperature stress. A large number of markers were significantly associated with various traits under heat stress. These included strong marker associations for number of inflorescence/plant (IPP), number of flowers/inflorescence (FPI), fresh fruit weight (FFrW), and electrolyte leakage (EL). High association with EL was found due to two SNPs 7858523|F|0-25:G>A-25:G>A and 4705224|F|0-60:C>G-60:C>G located on Chr 6. Other less pronounced marker-trait associations were observed for plant dry weight (PDW), and number of fruit/plant (FrPP).

Agronomic, physiological and molecular characterisation of rice mutants revealed the key role of reactive oxygen species and catalase in high-temperature stress tolerance

2020 ◽  
Vol 47 (5) ◽  
pp. 440 ◽  
Author(s):  
Syed Adeel Zafar ◽  
Amjad Hameed ◽  
Muhammad Ashraf ◽  
Abdus Salam Khan ◽  
Zia-ul- Qamar ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Heat Stress ◽  
High Temperature ◽  
Grain Yield ◽  
Temperature Stress ◽  
Heat Tolerance ◽  
High Temperature Stress ◽  
Oxygen Species ◽  
Selection Indices ◽  
Heat Tolerant

Climatic variations have increased the occurrence of heat stress during critical growth stages, which negatively affects grain yield in rice. Plants adapt to harsh environments, and particularly high-temperature stress, by regulating their physiological and biochemical processes, which are key tolerance mechanisms. The identification of heat-tolerant rice genotypes and reliable selection indices are crucial for rice improvement programs. Here, we evaluated the response of a rice mutant population for high-temperature stress at the seedling and reproductive stages based on agronomic, physiological and molecular indices. Estimates of variance components revealed significant differences (P < 0.001) among genotypes, treatments and their interactions for almost all traits. The principal component analysis showed significant diversity among genotypes and traits under high-temperature stress. The mutant HTT-121 was identified as the most heat-tolerant mutant with higher grain yield, panicle fertility, cell membrane thermo-stability (CMTS) and antioxidant enzyme levels under heat stress. Various seedling-based morpho-physiological traits (leaf fresh weight, relative water contents, malondialdehyde, CMTS) and biochemical traits (superoxide dismutase, catalase and hydrogen peroxide) explained variations in grain yield that could be used as selection indices for heat tolerance in rice during early growth. Notably, heat-sensitive mutants accumulated reactive oxygen species, reduced catalase activity and upregulated OsSRFP1 expression under heat stress, suggesting their key roles in regulating heat tolerance in rice. The heat-tolerant mutants identified in this study could be used in breeding programs and to develop mapping populations to unravel the underlying genetic architecture for heat-stress adaptability.

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