scholarly journals Occurrence, isolation, and identification of Acidovorax citrulli from Melon in Turkey

2014 ◽  
Vol 50 (No. 4) ◽  
pp. 179-183 ◽  
Author(s):  
S. Horuz ◽  
R. Cetinkaya-Yildiz ◽  
M. Mirik ◽  
Y. Aysan
Keyword(s):  
Eastern Mediterranean ◽  
Eastern Mediterranean Region ◽  
Isolation And Identification ◽  
New Host ◽  
Causal Organism ◽  
Plant Materials ◽  
Small Water ◽  
Acidovorax Citrulli ◽  
Initial Symptoms ◽  
Production Areas

During February and August of 2010 and 2011, disease symptoms were detected in melon (Cucumis melo cv. Surmeli) fields and commercial nurseries in Adana and Mersin provinces (Eastern Mediterranean Region, Turkey). Lesions on leaves and fruits were observed in nearly 75 and 85 acres production areas of melon in 2010 and 2011, respectively. Initial symptoms were water-soaked irregular spots, light brown to reddish spots advancing through veins on leaves, small water-soaked lesions, greasy, dark olive green colour areas on the surfaces of melon fruit followed by brown lesions, softening, and cracks. Thirty-five non-fluorescent, slow-growing, round, cream and Gram-negative bacterial isolates were isolated from symptomatic plant materials. The pathogenicity of the isolates was proved using melon seedlings and fruits. According to classical, serological, and molecular assays, the causal organism was identified as Acidovorax citrulli. To the best of our knowledge, this is the first report and occurrence of bacterial fruit blotch disease on melon as a new host in Turkey.  

scholarly journals Biological control of watermelon seedling blight caused by Acidovorax citrulli using antagonistic bacteria from the genera Curtobacterium, Microbacterium and Pseudomonas

2018 ◽  
Vol 54 (No. 3) ◽  
pp. 138-146 ◽  
Author(s):  
Horuz Sumer ◽  
Aysan Yesim
Keyword(s):  
Biological Control ◽  
Pseudomonas Fluorescens ◽  
Eastern Mediterranean ◽  
Disease Incidence ◽  
Eastern Mediterranean Region ◽  
Dual Culture ◽  
Antagonistic Bacteria ◽  
Seedling Blight ◽  
Acidovorax Citrulli ◽  
Microbacterium Oxydans

The biological control of the watermelon seedling blight and fruit blotch disease was investigated by screening the potential use of antagonistic bacteria. Between May and August 2012, totally 322 putative antagonistic bacteria were isolated from symptomless melon and watermelon plants grown in Adana, Hatay, and Osmaniye provinces of the Eastern Mediterranean Region of Turkey. In vitro dual culture tests showed that 54 out of 322 strains inhibited the Acidovorax citrulli (Ac) growth with an appearance of clear zones between 2.3 and 27.0 mm in diameter. However, the remaining 268 strains did not exhibit any antagonistic activity against Ac. Seed treatments with fourteen individual antagonistic bacteria resulted in a significant reduction in disease incidence (DI) and severity (DS) ranging between 14.06–79.47% and between 4.57–41.49%, respectively. The bacteria Pseudomonas oryzihabitans (Antg-12), Microbacterium oxydans (Antg-57), Curtobacterium flaccumfaciens (Antg-198), and Pseudomonas fluorescens (Antg-273) were the most potent antagonistic bacterial isolates which reduced DI and DS as compared to the untreated control. This study suggested the potential of bacterial antagonists Curtobacterium flaccumfaciens, Microbacterium oxydans, Pseudomonas oryzihabitans, and Pseudomonas fluorescens for the biocontrol of Ac-induced bacterial fruit blotch (BFB).

scholarly journals Identification of Pomegranate as a New Host of Passiflora Edulis Symptomless Virus (PeSV) and Analysis of PeSV Diversity

Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1821
Author(s):  
Kadriye Caglayan ◽  
Mona Gazel ◽  
Vahid Roumi ◽  
Hamide Deniz Kocabag ◽  
Bahar Tunç ◽  
...  
Keyword(s):  
High Throughput Sequencing ◽  
Eastern Mediterranean ◽  
Eastern Mediterranean Region ◽  
Rna Seq ◽  
New Host ◽  
Passiflora Edulis ◽  
Reference Isolate ◽  
Viral Particles ◽  
The Impact ◽  
India And China

Pomegranate is an important crop in the Mediterranean Basin that can be affected by a range of pathogens. With the aim to better understand the impact of viral diseases on pomegranate, two leaf samples from Turkey showing virus-like symptoms such as chlorotic spots and oak-leaf patterns were subjected to high throughput sequencing (HTS). Data analysis indicated the presence of passiflora edulis symptomless virus (PeSV: genus Roymovirus, Potyviridae family) in these two pomegranate samples, consistent with the observation by electron microscopy of flexuous filamentous viral particles 760 to 780 nm long. Further analysis of HTS reads revealed the presence of five PeSV variants in one of the samples and another single variant in the other. PeSV occurrence was also identified from publicly available SRA pomegranate RNA-Seq transcriptomic data from India and China. The genome of these PeSV-pomegranate variants share 78.0–86.8% nucleotide identity with that of the reference isolate from passionfruit (MH379332). The presence of PeSV in pomegranate was confirmed by specific RT-PCR assays targeting either the coat protein (CP) or Nla-Pro genes in 37 cultivated and one ornamental pomegranate out of 133 samples collected from the Eastern Mediterranean region of Turkey. To our knowledge, this is the first application of HTS to assess virus occurrence in pomegranate and the first recognition of pomegranate as a new host for PeSV.

scholarly journals Global Prevalence Estimates of Toxascaris leonina Infection in Dogs and Cats

Pathogens ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 503
Author(s):  
Ali Rostami ◽  
Seyed Mohammad Riahi ◽  
Vahid Fallah Omrani ◽  
Tao Wang ◽  
Andreas Hofmann ◽  
...  
Keyword(s):  
Mediterranean Region ◽  
Eastern Mediterranean ◽  
Meta Analysis ◽  
Eastern Mediterranean Region ◽  
Global Prevalence ◽  
Stray Cats ◽  
Prevalence Estimates ◽  
Meta Regression ◽  
Paratenic Hosts ◽  
Toxascaris Leonina

Toxascaris leonina is an ascaridoid nematode of dogs and cats; this parasite affects the health of these animals. This study estimated the global prevalence of Ta. leonina infection in dogs and cats using random effects meta-analysis as well as subgroup, meta-regression and heterogeneity analyses. The data were stratified according to geographical region, the type of dogs and cats and environmental variables. A quantitative analysis of 135 published studies, involving 119,317 dogs and 25,364 cats, estimated prevalence rates of Ta. leonina in dogs and cats at 2.9% and 3.4%, respectively. Prevalence was highest in the Eastern Mediterranean region (7.2% for dogs and 10.0% for cats) and was significantly higher in stray dogs (7.0% vs. 1.5%) and stray cats (7.5% vs. 1.8%) than in pets. The findings indicate that, worldwide, ~26 million dogs and ~23 million cats are infected with Ta. leonina; these animals would shed substantial numbers of Ta. leonina eggs into the environment each year and might represent reservoirs of infection to other accidental or paratenic hosts. It is important that populations of dogs and cats as well as other canids and felids be monitored and dewormed for Ta. leonina and (other) zoonotic helminths.

scholarly journals Primary care healthcare policy implementation in the Eastern Mediterranean region; experiences of six countries: Part II

2019 ◽  
Vol 26 (1) ◽  
pp. 1-6
Author(s):  
Nagwa Nashat ◽  
Redouane Hadjij ◽  
Abdul Munem Al Dabbagh ◽  
Mohammed Rasoul Tarawneh ◽  
Huda Alduwaisan ◽  
...  
Keyword(s):  
Primary Care ◽  
Policy Implementation ◽  
Mediterranean Region ◽  
Eastern Mediterranean ◽  
Eastern Mediterranean Region ◽  
Healthcare Policy

scholarly journals Correction to: A systematic review on hospital inefficiency in the Eastern Mediterranean Region: sources and solutions

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Hamid Ravaghi ◽  
Mahnaz Afshari ◽  
Parvaneh Isfahani ◽  
Victoria D. Bélorgeot
Keyword(s):  
Systematic Review ◽  
Mediterranean Region ◽  
Eastern Mediterranean ◽  
Eastern Mediterranean Region ◽  
Original Publication

In the original publication of this article [1], one author’s name needs to be revised from Pavaneh Isfahani to Parvaneh Isfahani.

scholarly journals COVID-19 Pandemic Affects the Medical Students’ Learning Process and Assaults Their Psychological Wellbeing

2021 ◽  
Vol 18 (11) ◽  
pp. 5792
Author(s):  
Marium Aftab ◽  
Alsaleem Mohammed Abadi ◽  
Shamsun Nahar ◽  
Razia Aftab Ahmed ◽  
Syed Esam Mahmood ◽  
...  
Keyword(s):  
Medical Students ◽  
Psychiatric Illness ◽  
Eastern Mediterranean ◽  
Learning Difficulties ◽  
Eastern Mediterranean Region ◽  
Female Gender ◽  
Psychological Wellbeing ◽  
Post Traumatic Stress ◽  
African Region ◽  
Cross Sectional

Background: With the emergence of the COVID-19 pandemic, people are living within a milieu of stress, anxiety, and fear. Medical students are susceptible to these emotional injuries, but their psychological wellbeing and learning may further be assaulted by future uncertainties and altered teaching and training programs. Our objective was to find the extent of the psychological impact of the pandemic and the learning difficulties they are experiencing; Methodology: This cross-sectional study included 418 undergraduate and postgraduate medical students from all over the world. A questionnaire was uploaded in Google survey form. It included background characteristics, questions for psychiatric impact like PHQ-9, GAD-7, ZF-OCS, and questions for learning difficulties perceived in comparison to the pre-pandemic time. Results: Among participants, 34.9% of students were male and 65.1% female. Around 46.4% belonged to the WHO, Eastern Mediterranean region, 26.8% from South East Asia region, 17.5% from the region of America, 5.5% from the European region,2.2%from the Western Pacific region, and 1.7% from the African region. Symptoms due to psychiatric illness were noticed in 393 (93.1%); depression in 386 (92.3%), anxiety in 158 (37.8%), obsessive compulsion disorder in 225 (53.8%), and post-traumatic stress syndrome in 129 (39.9%). Female gender, geographical region, and history of previous psychiatric illness were significantly related to almost all the psychiatric illnesses. Regarding learning difficulty, 96% of students faced problems: trouble with memorizing in 54.0%, concentration problems in 67.0%, about 55.5% of students made more mistakes, while 44.5% noted an increase in reaction time for solving questions. In addition, 90% experienced greater difficulty in overall learning during the pandemic in comparison to the pre-pandemic time. Conclusion: Assault on psychological wellbeing, struggling to memorize, inattention and difficulty in concentration on studies, along with perceived overall trouble with learning, have emerged as collateral damage from the COVID-19 pandemic with respect to medical students.

scholarly journals Effectiveness of Community Nutrition-Specific Interventions on Improving Malnutrition of Children under 5 Years of Age in the Eastern Mediterranean Region: A Systematic Review and Meta-Analysis

2021 ◽  
Vol 18 (15) ◽  
pp. 7844
Author(s):  
Delaram Ghodsi ◽  
Nasrin Omidvar ◽  
Bahareh Nikooyeh ◽  
Roshanak Roustaee ◽  
Elham Shakibazadeh ◽  
...  
Keyword(s):  
Cost Effectiveness ◽  
Mediterranean Region ◽  
Eastern Mediterranean ◽  
Meta Analysis ◽  
Eastern Mediterranean Region ◽  
Child Malnutrition ◽  
Z Score ◽  
Community Based ◽  
Nutritional Status Of Children ◽  
Children Under 5

Childhood malnutrition remains an important public health and development problem in low- and middle-income countries. This study aimed to systematically review the community-based nutrition-specific interventions and their effectiveness and/or cost-effectiveness on the nutritional status of children under 5 years of age in the Eastern Mediterranean Region (EMR). A systematic literature search of the English electronic databases, including PubMed, Scopus, ISI Web of Knowledge, Ovid, EMBASE, as well as Persian databases (SID and Magiran) was performed up to May 2019. Studies regarding the effectiveness/cost-effectiveness of the community-based nutrition-specific programs and interventions targeted at under-five-year children in EMR countries were selected. The primary outcomes were mean of Weight-for-age z-score (WAZ), Height-for-Age z-score (HAZ), and Weight-for-Height z-score (WHZ) of children or prevalence of wasting, stunting, and/or underweight among the children. Meta-analysis was also performed on the selected articles and intervention effects (mean differences) were calculated for each outcome for each study and pooled using a weighted random effects model. Risk of bias (ROB) of each included study was assessed based on the Cochrane Handbook for Systematic Reviews. The study protocol was registered in PROSPERO (CRD42020172643). Of 1036 identified studies, eight met the inclusion criteria. Amongst these, seven were from Pakistan and one from Iran. Only one study conducted in Pakistan reported the cost-effectiveness of nutrition-specific interventions in the region. Nutrition education/consultation and cash-based interventions were the most common nutrition-specific strategies used for management of child malnutrition in the EMR countries. Out of these eight studies, four were included in the meta-analysis. When different interventions were pooled, they had resulted in a significant improvement in WHZ of children (MD: 0.26; 95% CI: 0.07 to 0.46, three studies, I2 82.40%). Considering the high prevalence of child malnutrition in a number of countries in the region, capacity building and investigation regarding the implementation of new approaches to improve nutritional status of children and their effect(s) and cost-effectiveness assessment are highly recommended.

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