scholarly journals No significant differences in rhizosphere bacterial communities between Bt maize cultivar IE09S034 and the near-isogenic non-Bt cultivar Zong31

2018 ◽  
Vol 64 (No. 9) ◽  
pp. 427-434
Author(s):  
Liang Jingang ◽  
Luan Ying ◽  
Jiao Yue ◽  
Xin Longtao ◽  
Song Xinyuan ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Growth Stage ◽  
Bacterial Population ◽  
Rhizosphere Soil ◽  
Potential Effect ◽  
Toxin Gene ◽  
Bt Maize ◽  
Commercial Cultivation ◽  
Rhizosphere Bacterial Community ◽  
Rhizosphere Bacterial Communities

The release of genetically modified (GM) crops has potential to alter the bacterial population within rhizosphere. Here, the potential effect of GM maize cv. IE09S034 containing the Cry1Ie toxin gene from Bacillus thuringiensis (Bt) was investigated under the field conditions. The community composition and the relative abundance of the bacteria in rhizosphere soil were estimated by analysing 16S rRNA PCR amplicons. Our results indicated that Bt maize IE09S034 has no significant effects on the rhizosphere bacterial community. Instead, it was found that factors such as plant growth stage and year have a stronger effect on the bacterial population dynamics. Our findings therefore provide reliable evidence supporting the potential commercial cultivation of the cv. IE09S034.

scholarly journals Variation of rhizosphere bacterial community diversity in the desert ephemeral plant Ferula sinkiangensis across environmental gradients

2020 ◽  
Author(s):  
Zhang tao ◽  
Wang Zhongke ◽  
Lv Xinhua ◽  
Dang Hanli ◽  
Zhuang Li
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Community Diversity ◽  
Bacterial Community Diversity ◽  
Physicochemical Factors ◽  
Rhizosphere Bacterial Community ◽  
Ferula Sinkiangensis ◽  
Rhizosphere Bacterial Communities

Ferula sinkiangensis is a desert short-lived medicinal plant, and its number is rapidly decreasing. Rhizosphere microbial community plays an important role in plant growth and adaptability. However, Ferula sinkiangensis rhizosphere bacterial communities and the soil physicochemical factors that drive the bacterial community distribution are currently unclear. On this study, based on high-throughput sequencing, we explored the diversity, structure and composition of Ferula sinkiangensis rhizosphere bacterial communities at different slope positions and soil depths and their correlation with soil physicochemical properties. Our results revealed the heterogeneity and variation trends of Ferula sinkiangensis rhizosphere bacterial community diversity and abundance on a fine spatial scale (Slope position and soil depth) and Found Actinobacteria (25.5%), Acidobacteria (16.9%), Proteobacteria (16.6%), Gemmatimonadetes (11.5%) and Bacteroidetes (5.8%) were the dominant bacterial phyla in Ferula sinkiangensi s rhizosphere soil. Among all soil physicochemical variables shown in this study, there was a strong positive correlation between phosphorus (AP) and the diversity of rhizosphere bacterial community in Ferula sinkiangensis . In addition, Soil physicochemical factors jointly explained 24.28% of variation in Ferula sinkiangensis rhizosphere bacterial community structure. Among them, pH largely explained the variation of Ferula sinkiangensis rhizosphere bacterial community structure (5.58%), followed by total salt (TS, 5.21%) and phosphorus (TP, 4.90%).

scholarly journals Variation of rhizosphere bacterial community diversity in the desert ephemeral plant Ferula sinkiangensis across environmental gradients

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Tao Zhang ◽  
Zhongke Wang ◽  
Xinhua Lv ◽  
Hanli Dang ◽  
Li Zhuang
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Community Diversity ◽  
Bacterial Community Diversity ◽  
Physicochemical Factors ◽  
Rhizosphere Bacterial Community ◽  
Ferula Sinkiangensis ◽  
Rhizosphere Bacterial Communities

Abstract Ferula sinkiangensis (F. sinkiangensis) is a desert short-lived medicinal plant, and its number is rapidly decreasing. Rhizosphere microbial community plays an important role in plant growth and adaptability. However, F. sinkiangensis rhizosphere bacterial communities and the soil physicochemical factors that drive the bacterial community distribution are currently unclear. On this study, based on high-throughput sequencing, we explored the diversity, structure and composition of F. sinkiangensis rhizosphere bacterial communities at different slope positions and soil depths and their correlation with soil physicochemical properties. Our results revealed the heterogeneity and changed trend of F. sinkiangensis rhizosphere bacterial community diversity and abundance on slope position and soil depth and found Actinobacteria (25.5%), Acidobacteria (16.9%), Proteobacteria (16.6%), Gemmatimonadetes (11.5%) and Bacteroidetes (5.8%) were the dominant bacterial phyla in F. sinkiangensis rhizosphere soil. Among all soil physicochemical variables shown in this study, there was a strong positive correlation between phosphorus (AP) and the diversity of rhizosphere bacterial community in F. sinkiangensis. In addition, Soil physicochemical factors jointly explained 24.28% of variation in F. sinkiangensis rhizosphere bacterial community structure. Among them, pH largely explained the variation of F. sinkiangensis rhizosphere bacterial community structure (5.58%), followed by total salt (TS, 5.21%) and phosphorus (TP, 4.90%).

scholarly journals Variation of rhizosphere bacterial community diversity in the desert ephemeral plant Ferula sinkiangensis across environmental gradients

2020 ◽  
Author(s):  
Zhang tao ◽  
Wang Zhongke ◽  
Lv Xinhua ◽  
Dang Hanli ◽  
Zhuang Li
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Community Diversity ◽  
Bacterial Community Diversity ◽  
Physicochemical Factors ◽  
Rhizosphere Bacterial Community ◽  
Ferula Sinkiangensis ◽  
Rhizosphere Bacterial Communities

Ferula sinkiangensis is a desert short-lived medicinal plant, and its number is rapidly decreasing. Rhizosphere microbial community plays an important role in plant growth and adaptability. However, Ferula sinkiangensis rhizosphere bacterial communities and the soil physicochemical factors that drive the bacterial community distribution are currently unclear. On this study, based on high-throughput sequencing, we explored the diversity, structure and composition of Ferula sinkiangensis rhizosphere bacterial communities at different slope positions and soil depths and their correlation with soil physicochemical properties. Our results revealed the heterogeneity and variation trends of Ferula sinkiangensis rhizosphere bacterial community diversity and abundance on a fine spatial scale (Slope position and soil depth) and Found Actinobacteria (25.5%), Acidobacteria (16.9%), Proteobacteria (16.6%), Gemmatimonadetes (11.5%) and Bacteroidetes (5.8%) were the dominant bacterial phyla in Ferula sinkiangensi s rhizosphere soil. Among all soil physicochemical variables shown in this study, there was a strong positive correlation between phosphorus (AP) and the diversity of rhizosphere bacterial community in Ferula sinkiangensis . In addition, Soil physicochemical factors jointly explained 24.28% of variation in Ferula sinkiangensis rhizosphere bacterial community structure. Among them, pH largely explained the variation of Ferula sinkiangensis rhizosphere bacterial community structure (5.58%), followed by total salt (TS, 5.21%) and phosphorus (TP, 4.90%).

scholarly journals Dynamic changes in the rhizosphere bacterial community in monoculture and intercropping maize and soybean during various crop growth stages 

2020 ◽  
Author(s):  
Han Li ◽  
Luyun Luo ◽  
Bin Tang ◽  
Huanle Guo ◽  
Zhongyang Cao ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Physicochemical Properties ◽  
Bacterial Communities ◽  
Rhizosphere Soil ◽  
Crop Growth ◽  
Soil Samples ◽  
Growth Stages ◽  
Soil Physicochemical Properties ◽  
Crop Growth Stages ◽  
Rhizosphere Bacterial Communities

Abstract Although rhizosphere microorganisms have been studied for a long time, rhizosphere microbial communities based on monoculture and intercropping soybean and maize have rarely been studied. To define the effect of crop monoculture and intercropping on soil physicochemical properties and rhizosphere bacterial communities, field experiments were conducted using maize and soybean cultivars at five different crop growth stages, including monoculture maize, monoculture soybean and maize-soybean intercropping. The rhizosphere bacterial communities were analyzed by using the 16S rRNA Illumina sequencing. The pH and soil organic matter (SOM) were the key factors affecting crop rhizosphere soil bacterial communities. The intercropping soybean-maize increased the available phosphorus (AP) content at five different crop growth stages. And the available potassium (AK) content in the intercropping soybean soil samples was higher than corresponding monoculture soil samples. The content of available cadmium (ACd) in monoculture soybean rhizosphere soil samples decreased and then increased, but the intercropping soybean soil samples indicated an opposite trend. Proteobacteria, Chloroflexi, Acidobacteria, Actinobacteria and Firmicutes were the dominant phyla in the soybean and maize rhizosphere soil samples. Crops of the same plant species showed little difference in the bacterial community diversity under the two planting modes. The results indicated the intercropping planting pattern altered the absorption of ACd in the maize and soybean soil since the S2 stage and showed a different change in different crop growth stages. And the maize-soybean intercropping system also changed the bacterial community and soil physicochemical properties.

scholarly journals Rhizosphere Bacterial Community Characteristics over Different Years of Sugarcane Ratooning in Consecutive Monoculture

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Xiaoning Gao ◽  
Zilin Wu ◽  
Rui Liu ◽  
Jiayun Wu ◽  
Qiaoying Zeng ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Bacterial Communities ◽  
Illumina Miseq ◽  
Miseq Sequencing ◽  
Community Characteristics ◽  
Dynamic Changes ◽  
Operational Taxonomic Units ◽  
Total Bacterial Community ◽  
Rhizosphere Bacterial Community ◽  
Rhizosphere Bacterial Communities

To understand dynamic changes in rhizosphere microbial community in consecutive monoculture, Illumina MiSeq sequencing was performed to evaluate the V3-V4 region of 16S rRNA in the rhizosphere of newly planted and three-year ratooning sugarcane and to analyze the rhizosphere bacterial communities. A total of 126,581 and 119,914 valid sequences were obtained from newly planted and ratooning sugarcane and annotated with 4445 and 4620 operational taxonomic units (OTUs), respectively. Increased bacterial community abundance was found in the rhizosphere of ratooning sugarcane when compared with the newly planted sugarcane. The dominant bacterial taxa phyla were similar in both sugarcane groups. Proteobacteria accounted for more than 40% of the total bacterial community, followed by Acidobacteria and Actinobacteria. The abundance of Actinobacteria was higher in the newly planted sugarcane, whereas the abundance of Acidobacteria was higher in the ratooning sugarcane. Our study showed that Sphingomonas, Bradyrhizobium, Bryobacter, and Gemmatimonas were dominant genera. Moreover, the richness and diversity of the rhizosphere bacterial communities slightly increased and the abundance of beneficial microbes, such as Bacillus, Pseudomonas, and Streptacidiphilus, in ratooning sugarcane were more enriched. With the consecutive monoculture of sugarcane, the relative abundance of functional groups related to energy metabolism, glycan biosynthesis, metabolism, and transcription were overrepresented in ratooning sugarcane. These findings could provide the way for promoting the ratooning ability of sugarcane by improving the soil bacterial community.

scholarly journals Analysis of Tea Rhizosphere Bacterial Community at the Seedling Stage Using Terminal Restriction Fragment Length Polymorphism (TRFLP) Techniques

2017 ◽  
Vol 19 (2) ◽  
Author(s):  
Dwi Ningsih Susilowati ◽  
Fani Fauziah ◽  
Eko Pranoto ◽  
Ernin Hidayat ◽  
Mamik Setyowati ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Bacterial Community ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Bacterial Communities ◽  
Fragment Length Polymorphism ◽  
Rhizosphere Bacterial Community ◽  
Tea Plants ◽  
Rhizosphere Bacterial Communities ◽  
Restriction Fragment Length

<em>Bio-imunizer</em><em> contains an active compound of  </em>Chryseobacterium<em> sp. and </em>Bacillus<em> sp. has been developed by PPTK Gambung. This formula has positive effect on the growth of tea plants also potentially increasing resistance of the plant. The purpose of this study was to determine the effects of bacteria in Bio-imunizer to the rhizosphere bacterial communities as well as the consistency of its existence after application on tea plants at the nursery stage. The technique used in this research is </em>Terminal Restriction Fragment Length Polymorphism <em>based on metagenomic and culture dependent approaches. The value of relative abundance, Shannon diversity index, Pielou's evenness index, and Simpson dominance index were calculated. Based on the T-RF profiles of rhizosphere bacterial communities show that </em>Chryseobacterium<em> sp. and </em>Bacillus<em> sp. which is the active compound of  Bio-imunizer consistently found in the tea plant rhizosphere. Application of Bio-imunizer can increase the diversity of rhizosphere bacterial community without affecting the communities that already exist.</em>

scholarly journals Differential Assembly and Shifts of the Rhizosphere Bacterial Community by a Dual Transgenic Glyphosate-Tolerant Soybean Line with and without Glyphosate Application

Horticulturae ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. 374
Author(s):  
Minkai Yang ◽  
Zhongling Wen ◽  
Chenyu Hao ◽  
Aliya Fazal ◽  
Yonghui Liao ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Bacterial Communities ◽  
High Throughput Sequencing ◽  
Economic Benefits ◽  
Rrna Gene ◽  
Soybean Line ◽  
Seed Filling ◽  
Alpha And Beta Diversity ◽  
Rhizosphere Bacterial Community ◽  
Rhizosphere Bacterial Communities

Modern agriculture has gained significant economic benefits worldwide with the use of genetically modified (GM) technologies. While GM crops provide convenience to humans, their biosafety has attracted increasing concern. In this study, the Illumina MiSeq was used to perform a high-throughput sequencing of the V3-V4 hypervariable regions of 16S rRNA gene (16S rDNA) amplicons to compare the rhizosphere bacterial communities of the EPSPS/GAT dual transgenic glyphosate-tolerant soybean line Z106, its recipient variety ZH10, and Z106 with glyphosate application (Z106G) during flowering, seed filling, and maturing stages under field settings. At each of the three stages, the alpha and beta diversity of rhizosphere bacterial communities revealed no significant differences between ZH10, Z106, and Z106G. However, some bacterial taxa demonstrated a greater proportional contribution, particularly the nitrogen-fixing rhizobium Ensifer fredii, in the rhizospheric soil of Z106 at the seed filling and maturing stages, when compared to ZH10 and Z106G. The present study therefore suggests that the EPSPS/GAT dual transgenic line Z106 and exogenous glyphosate application have a minimal effect on the composition of the soybean rhizosphere bacterial community but have no impact on the structure of the rhizosphere microbial community during a single planting season.

scholarly journals Variation of rhizosphere bacterial community diversity in the desert ephemeral plant Ferula sinkiangensis across environmental gradients

2020 ◽  
Author(s):  
Zhang Tao ◽  
Dang Han Li ◽  
Wang Zhong Ke ◽  
Lv Xin Hua ◽  
Zhuang Li
Keyword(s):  
Bacterial Community ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Environmental Gradients ◽  
Soil Depth ◽  
Community Diversity ◽  
Slope Position ◽  
Rhizosphere Bacterial Community ◽  
Ferula Sinkiangensis ◽  
Rhizosphere Bacterial Communities

Abstract Background Ferula sinkiangensis is a desert short-lived medicinal plant, and its number is rapidly decreasing. Rhizosphere microbial community plays an important role in regulating global biogeochemical cycle, plant growth and adaptability. However, the Ferula sinkiangensis bacterial community and the processes that drive its assembly remain unclear. Results In this study, based on Illumina HiSeq high-throughput sequencing, we explored the diversity, structure and composition of Ferula sinkiangensis rhizosphere bacterial communities at different slope positions (upper, middle and bottom) and soil depths (0-10 cm, 10-25 cm, 25-40 cm) and their correlation with soil physicochemical properties. Actinobacteria (22.7%), Proteobacteria (18.6%), Acidobacteria (14.0%), Gemmatimonadetes (10.1%), Cyanobacteria (7.9%), Bacteroidetes (6.9%), Planctomycetes (3.9%), Verrucomicrobia (3.5%), Firmicutes (3.4%) and Chloroflexi (3.2%) were the dominant bacterial phyla in Ferula sinkiangensis rhizosphere soil. Variance analysis showed that the diversity and abundance of rhizosphere bacterial community in Ferula sinkiangensis were significantly different at various slope positions and soil depths. Specifically, the diversity of bacterial community was significantly higher at the top than the bottom of the slope, and the diversity and richness of bacterial community were significantly greater in the 0-10cm than the 25-40cm soil layer. Linear discriminant effect size (LEfSe) analysis showed the specific phyla and genera of bacteria affected by slope position and soil depth. For example, Planctomycetes, Sphingomonas , Rubrobacter and Adhaeribacter by slope position and significant impact on soil depth. In addition, distance-based redundancy analysis (db-RDA) and variance analysis showed that soil physicochemical factors jointly explained 29.81% of variation in Ferula sinkiangensis rhizosphere bacterial community structure. There was a significant positive correlation between available phosphorus（AP）and the diversity of Ferula sinkiangensis rhizosphere bacterial community ( p < 0.01), whereas pH largely explained the variation of Ferula sinkiangensis rhizosphere bacterial community structure (5.58%, p < 0.01), followed by altitude (5.53%), total salt (TS, 5.21%) and total phosphorus (TP, 4.90%). Conclusion Our results revealed the heterogeneity and variation trends of Ferula sinkiangensis rhizosphere bacterial community diversity and abundance on a fine spatial scale (slope position and depth) and shed new light on the mechanisms of coevolution and interaction between Ferula sinkiangensis and their rhizosphere bacterial communities across environmental gradients.

scholarly journals Variation of rhizosphere bacterial community diversity in the desert ephemeral plant Ferula sinkiangensis across environmental gradients

2020 ◽  
Author(s):  
Zhang tao ◽  
Wang Zhongke ◽  
Lv Xinhua ◽  
Dang Hanli ◽  
Zhuang Li
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Community Diversity ◽  
Bacterial Community Diversity ◽  
Physicochemical Factors ◽  
Rhizosphere Bacterial Community ◽  
Ferula Sinkiangensis ◽  
Rhizosphere Bacterial Communities

Ferula sinkiangensis ( F.sinkiangensis ) is a desert short-lived medicinal plant, and its number is rapidly decreasing. Rhizosphere microbial community plays an important role in plant growth and adaptability. However, F.sinkiangensis rhizosphere bacterial communities and the soil physicochemical factors that drive the bacterial community distribution are currently unclear. On this study, based on high-throughput sequencing, we explored the diversity, structure and composition of F.sinkiangensis rhizosphere bacterial communities at different slope positions and soil depths and their correlation with soil physicochemical properties. Our results revealed the heterogeneity and changed trend of F.sinkiangensis rhizosphere bacterial community diversity and abundance on slope position and soil depth and found Actinobacteria (25.5%), Acidobacteria (16.9%), Proteobacteria (16.6%), Gemmatimonadetes (11.5%) and Bacteroidetes (5.8%) were the dominant bacterial phyla in F.sinkiangensis rhizosphere soil. Among all soil physicochemical variables shown in this study, there was a strong positive correlation between phosphorus (AP) and the diversity of rhizosphere bacterial community in F.sinkiangensis . In addition, Soil physicochemical factors jointly explained 24.28% of variation in F.sinkiangensis rhizosphere bacterial community structure. Among them, pH largely explained the variation of F.sinkiangensis rhizosphere bacterial community structure (5.58%), followed by total salt (TS, 5.21%) and phosphorus (TP, 4.90%).

Isolation of Rhizosphere Bacterial Communities from Soil

BIO-PROTOCOL ◽  
2015 ◽  
Vol 5 (16) ◽  
Author(s):  
Laura White ◽  
Volker Br�zel ◽  
Senthil Subramanian
Keyword(s):  
Bacterial Communities ◽  
Rhizosphere Bacterial Communities
Sign in / Sign up

Export Citation Format

Share Document