scholarly journals Effect of rumen-protected methionine, lysine or both on milk production and plasma amino acids of high-yielding dairy cows

2009 ◽  
Vol 54 (No. 6) ◽  
pp. 239-248 ◽  
Author(s):  
J. Třináctý ◽  
L. Křížová ◽  
M. Richter ◽  
V. Černý ◽  
J. Říha
Keyword(s):  
Amino Acids ◽  
Dairy Cows ◽  
Milk Production ◽  
Latin Square ◽  
Experimental Period ◽  
Blood Metabolites ◽  
Plasma Amino Acids ◽  
Maize Silage ◽  
Beta Casein ◽  
Copolymer Coating

The objective of this study was to determine the effect of supplemental lysine (Lys), methionine (Met) or both amino acids added in the form of rumen-protected (RP) tablets with copolymer coating to a diet of dairy cows on yield and composition of milk and concentration of plasma amino acids (AA). The experiment was carried out on four high-yielding lactating Holstein cows with average milk production of 33.5 kg/day in the form of Latin square design. The four treatments were as follows: C – control without AA supplementation, L – control plus supplement of RP Lys, M – control plus supplement of RP Met and ML – control plus supplement of RP Met and Lys. The experiment was divided into 4 periods. Each period (14 days) consisted of 10-day preliminary period and 4-day experimental period. Cows were fed a diet based on maize silage, lucerne hay and supplemental mixture. Average milk yield recorded in ML was 34.18 kg and was higher than that recorded in L or M (32.46 kg and 32.13 kg, respectively <I>P</I> < 0.05) and tended to be higher than in C (33.33 kg, <I>P</I> > 0.05). The content of protein and casein was higher in L and ML in comparison with C (<I>P</I> < 0.05) and tended to be higher than in M (<I>P</I> > 0.05). Protein yield in ML (1 054 g/day) was higher than that found in C, L or M (990, 998 or 968 g/day, respectively, <I>P</I> < 0.05). The same response was found for casein yield (<I>P</I> < 0.05). Although the proportion of individual casein fractions was not affected by the treatment, the yield of &alpha;- and &beta;-casein differed (<I>P</I> < 0.05) while the yield of &kappa;-casein was not affected by the treatment. Concentrations of blood metabolites, except for betahydroxybutyrate (BHB), were not changed. Plasma Met concentrations were increased (<I>P</I> < 0.05) in the M and ML group. Similar, but insignificant increases (<I>P</I> > 0.05) were also observed in plasma Lys in the L and ML group.

scholarly journals Effect of duodenal infusions of leucine on milk yield and plasma amino acids in dairy cows

2010 ◽  
Vol 55 (No. 9) ◽  
pp. 351-358 ◽  
Author(s):  
M. Richter ◽  
J. Svobodová ◽  
L. Křížová ◽  
J. Třináctý ◽  
P. Homolka
Keyword(s):  
Amino Acids ◽  
Dairy Cows ◽  
Milk Yield ◽  
Dry Matter ◽  
Basal Diet ◽  
Experimental Period ◽  
Plasma Amino Acids ◽  
Maize Silage ◽  
Subsequent Period ◽  
Period 2

Four high-yielding lactating Holstein cows fitted with duodenal cannulas were used in the experiment. Cows were divided into 2 groups &ndash; control (Control) with leucine deficiency and experimental (Leucine) with a leucine supplement. The experiment was divided into 4 periods of 7 days, each consisting of a 3-day preliminary period followed by a 4-day experimental period. In the first period, 2 cows were assigned to Control and the remaining 2 to Leucine. In the subsequent period the cows were switched to the other treatment. Cows were fed individually twice daily the basal diet based on maize silage, lucerne hay and supplemental mixture. Infusions of amino acids in Leucine consisted of methionine (12.6 g/day), lysine (20.7 g/day), histidine (10.7 g/day) and leucine (19.3 g/day). The composition of amino acid infusate in Control was the same except for leucine that was replaced with monosodium L-glutamate. The intake of dry matter was not affected by the treatment (P &gt; 0.05). No effect of leucine infusion on milk yield and composition was observed (P &gt; 0.05), nevertheless the concentration of protein and casein in milk tended to be higher in Leucine (38.3 and 31.3 g/kg) than in Control (37.4 and 30.4 g/kg, respectively, P &lt; 0.1). The yield of milk components was not affected by the treatment (P &gt; 0.05). Duodenal infusion of leucine resulted in a decreased plasma level of isoleucine in Leucine compared to Control (P &lt; 0.01). Concentrations of leucine, cysteine and citrulline tended to be higher and the concentration of tyrosine tended to be lower in Leucine in comparison with Control (P &lt; 0.10).

scholarly journals Effect of Ruminally-protected leucine supplement on milk yield and plasma amino acids in dairy cows

2008 ◽  
Vol 17 (4) ◽  
pp. 351 ◽  
Author(s):  
L. KRÍZOVÁ ◽  
J. TRINÁCTÝ ◽  
M. RICHTER
Keyword(s):  
Amino Acids ◽  
Milk Yield ◽  
Dry Matter ◽  
Milk Protein ◽  
Experimental Period ◽  
Dry Matter Intake ◽  
Plasma Amino Acids ◽  
Maize Silage ◽  
Milk Protein Content ◽  
Ad Libitum

The aim of this study was to determine the influence of leucine supplement in the form of rumen-protected tablets on milk yield and composition and plasma amino acids in four high-yielding lactating Holstein cows. The experiment was carried out as a cross-over procedure and was divided into 4 periods of 14 d (10 d preliminary period and 4 d experimental period). Cows were fed ad libitum a diet based on maize silage, lucerne hay and a supplemental mixture. The diet, defficient in methionine, lysine, and leucine, was supplemented with methionine+lysine (Control) or methionine+lysine+leucine (Leu) in rumen protected form. The dry matter intake, milk yield and milk yield expressed in energy corrected milk did not differ significantly between the treatments. Milk protein content and yield did not show statistically significant variation. The contents and yield of casein, fat, lactose and urea were unaffected by the treatment. Blood metabolites did not vary between the treatments. The introduction of Leu resulted in higher plasma levels of proline (p

scholarly journals Effect of supplemental rumen-protected lysine, methionine or both added to diet of lactating dairy cows on milk fatty acids profile

2010 ◽  
Vol 58 (1) ◽  
pp. 87-94 ◽  
Author(s):  
Ludmila Křížová ◽  
Jiří Třináctý ◽  
Jarmila Svobodová ◽  
Michal Richter ◽  
Vladimír Černý ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Milk Fat ◽  
Latin Square ◽  
Total Content ◽  
Experimental Period ◽  
Maize Silage ◽  
Milk Fatty Acids ◽  
Fatty Acids Profile ◽  
Lactating Dairy Cows

The objective of this study was to determine the effect of supplemental lysine (Lys), methionine (Met) or both added to diet of dairy cows in the form of rumen-protected (RP) tablets on changes in milk fatty acids (FA) profile. The trial was carried out on four lactating Holstein cows in the form of Latin square design and was divided into 4 periods of 14 d (10-d preliminary period and a 4-d experimental period). The four treatments were as follows: C – control without amino acids (AA) supplementation, L – supplement of RP Lys, M – supplement of RP Met and ML – supplement of RP Met and Lys. Cows were fed on a diet based on maize silage, lucerne hay and supplemental mixture. Milk yield in ML (34.18 kg/d) was higher than in L or M (32.46 kg and 32.13 kg, respectively, P < 0.05) and tended to be higher than in C (33.33 kg/d, P > 0.05). Protein yield in ML (1054 g / d) was higher than that found in C, L or M (990, 998 or 968 g / d, respectively, P < 0.05). Milk fat content and yield in C and ML was higher in comparison to L and M (P < 0.05). Content of short-chain FA (C 4:0–C 12:0) was not affected by the treatment except of L that was lower than in C (P < 0.05). Content of medium-chain FA in M was lower compared to C, L or ML (P < 0.05). The content of long-chain FA in M was significantly higher than in other groups (P < 0.05). The total content of SFA in M was lower than in C or ML (P < 0.05) and tended to be lower than in L. Contents of UFA, MUFA and PUFA in M were higher than in C and ML (P < 0.05).

Pasture intake and milk production of dairy cows grazing annual ryegrass with or without corn silage supplementation

2014 ◽  
Vol 54 (10) ◽  
pp. 1810 ◽  
Author(s):  
Marcolino Frederico Miguel ◽  
Henrique Mendonça Nunes Ribeiro-Filho ◽  
Ederson Américo de Andrade ◽  
Teresa Cristina Moraes Genro ◽  
Rémy Delagarde
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Substitution Rate ◽  
Lolium Multiflorum ◽  
Latin Square ◽  
Ground Level ◽  
Experimental Period ◽  
Individual Performance ◽  
Corn Silage ◽  
Annual Ryegrass

The use of corn silage to supplement grazing dairy cows can improve milk production and nutrient intake when the substitution rate is low. This enhancement occurs by increasing the total DM intake. The hypothesis tested in this study was that increasing corn silage supplementation level of dairy cows grazing annual ryegrass at medium pasture allowance could increase substitution rate and decrease milk production response. Three supplementation levels (0, 4 and 8 kg DM/day) were compared for dairy cows strip-grazing annual ryegrass (Lolium multiflorum cv. Common). The study was arranged as a 3 × 3 Latin square design, repeated three times using nine Holstein lactating cows. The supplement was a 7 : 1 mixture based on the DM of corn silage and soybean meal and was offered individually twice daily after the morning and afternoon milkings. Each treatment group of cows grazed separately at a common daily pasture allowance of 35 kg DM/cow to ground level. Each experimental period was 12 days, with an 8-day adaptation and a 4-day measurement period. The individual pasture intake was measured from Day 9 to Day 12 using the n-alkane technique. The daily pattern of grazing and ruminating times were measured by visual observations. The supplement DM intake was lower than expected, averaging 3.0 and 4.3 kg DM/day for cows receiving 4 and 8 kg DM/day, respectively. Pasture and supplement energy value were 7.0 and 6.3 MJ of NEL/kg DM, respectively. The substitution rate averaged 0.2 and was unaffected by the supplementation level. The pasture intake was similar for all treatments (14.0 kg of DM/day), and total DM intake and milk production (+0.5 kg of milk/kg DM of supplement) increased linearly with increasing supplementation level. The daily grazing time was shorter by 111 min/day for the supplemented cows than for the unsupplemented cows. Cows grazing the annual ryegrass at medium pasture allowance improved their total DM intake and individual performance when supplemented with corn silage.

scholarly journals Organic supplementation of Cu and Se in the diets of Normande dairy cows

2020 ◽  
Vol 26 (1) ◽  
pp. e1983
Author(s):  
Pedro N. Rodriguez-Hernandez ◽  
Diana Cediel-Devia ◽  
Román David Castañeda-Serrano
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Experimental Period ◽  
Milk Quality ◽  
Blood Metabolites ◽  
Reproductive Parameters ◽  
Mineral Supplementation ◽  
Organic Copper ◽  
Inorganic Mineral ◽  
Organic Minerals

Objective: to evaluate the effects of organic copper (Cu) and selenium (Se) on milk production, milk quality and reproductive parameters of Normande dairy cows. Materials and methods: 36 multiparous cows (BW = 600 ± 32 kg) were used, distributed in completely randomized blocks. Treatments were: T0: without mineral supplementation, T1: inorganic mineral supplementation with Cu and Se, T2: 50% Inorganic supplementation + 50% organic supplementation, with an experimental period of 150 days (30 days before calving and 120 days of lactation). The variables evaluated were milk production and quality (total solids, proteins, fat, somatic cells and MUN), blood metabolites (glucose, betahydroxybutyrate and BUN) and reproductive parameters (open days, days to first service and services per conception). Results: No differences were observed in milk production and quality (P> 0.05). However, open days and services per conception were shorter when cows were supplemented with organic minerals (P <0.05). Conclusions: In this study milk production, milk quality and blood metabolites were not affected by supplementation with Se and Cu. However, the reproductive parameters of the cows improved when supplemented with organic Se and Cu.

scholarly journals Milk production and concentrations of blood metabolites as influenced by the level of wet distiller’s solubles in dairy cows receiving grass silage-based diet

1992 ◽  
Vol 1 (3) ◽  
pp. 279-290
Author(s):  
Pekka Huhtanen ◽  
Harri Miettinen
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Feed Intake ◽  
Dry Matter ◽  
Latin Square ◽  
Blood Metabolites ◽  
Blood Constituents ◽  
Grass Silage ◽  
Ad Libitum ◽  
Dietary Treatments

Twelve Finnish Ayrshire cows were used in a 4 x 4 Latin square design with 4-week experimental periods to study the effects of replacing increasing amount of barley with wet distiller’s solubles (WDS) on feed intake, milk production, digestibility and blood constituents. The four dietary treatments consisted of grass silage ad libitum and 7.8 kg dry matter (DM)/d of barley, of which 0 (WDSO), 1 (WDS1), 2 (WDS2) and 3 kg DM/d (WDS3) was replaced with WDS. Mainly because of a greater intake of concentrate with WDS containing diets silage DM intake varied quadratically (P

scholarly journals Metabolic and Productive Response and Grazing Behavior of Lactating Dairy Cows Supplemented with High Moisture Maize or Cracked Wheat Grazing at Two Herbage Allowances in Spring

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 919
Author(s):  
Verónica M. Merino ◽  
Lorena Leichtle ◽  
Oscar A. Balocchi ◽  
Francisco Lanuza ◽  
Julián Parga ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Fat ◽  
Dry Matter ◽  
Body Condition Score ◽  
Latin Square ◽  
Ruminal Fermentation ◽  
High Moisture ◽  
Plasma Urea ◽  
Grazing Behavior

The aim was to determine the effect of the herbage allowance (HA) and supplement type (ST) on dry matter intake (DMI), milk production and composition, grazing behavior, rumen function, and blood metabolites of grazing dairy cows in the spring season. Experiment I: 64 Holstein Friesian dairy cows were distributed in a factorial design that tested two levels of daily HA (20 and 30 kg of dry matter (DM) per cow) and two ST (high moisture maize (HMM) and cracked wheat (CW)) distributed in two daily rations (3.5 kg DM/cow/day). Experiment II: four mid-lactation rumen cannulated cows, supplemented with either HMM or CW and managed with the two HAs, were distributed in a Latin square design of 4 × 4, for four 14-d periods to assess ruminal fermentation parameters. HA had no effect on milk production (averaging 23.6 kg/day) or milk fat and protein production (823 g/day and 800 g/day, respectively). Cows supplemented with CW had greater protein concentration (+1.2 g/kg). Herbage DMI averaged 14.17 kg DM/cow.day and total DMI averaged 17.67 kg DM/cow.day and did not differ between treatments. Grazing behavior activities (grazing, rumination, and idling times) and body condition score (BCS) were not affected by HA or ST. Milk and plasma urea concentration increased under the high HA (+0.68 mmol/L and +0.90 mmol/L, respectively). Cows supplemented with HMM had lower milk and plasma urea concentrations (0.72 mmol/L and 0.76 mmol/L less, respectively) and tended (p = 0.054) to have higher plasma β-hydroxybutyrate. Ruminal parameters did not differ between treatments.

The Effect of Level of Supplement Feeding and Source of Protein on Milk Production and Nutrient Flows of Dairy Cows

1994 ◽  
Vol 1994 ◽  
pp. 142-142
Author(s):  
F.P. O'Mara ◽  
J.J. Murphy ◽  
M. Rath
Keyword(s):  
Protein Synthesis ◽  
Dairy Cows ◽  
Milk Production ◽  
Milk Protein ◽  
Solid Phase ◽  
Latin Square ◽  
Nutrient Flows ◽  
Grass Silage ◽  
Bacterial Marker ◽  
Friesian Cows

Milk protein synthesis may be limited by amino acid (AA) flow to the duodenum. This can be increased by increasing the flow of microbial AA's or undegraded feed AA's. This experiment was carried out to determine the effect on milk production and nutrient flows at the duodenum of including fishmeal (120g/kg) in the supplement to grass silage at two levels of supplement feeding.The treatments, arranged in a 2x2 factorial, were 1.) 3.5 kg/day of 0% fishmeal supplement (L-UDP), 2.) 7 kg/day of L-UDP, 3.) 3.5 kg/day of 12% fishmeal supplement (H-UDP), and 4.) 7 kg/day of H-UDP. Supplements were fed to 3 6 Friesian cows in a 4x4 multiple Latin-square trial with three week periods to determine production responses, and to four ruminally and duodenally cannulated cows to determine rumen fermentation and nutrient flows. Flows were determined by the dual marker technique of Faichney (1975) using cobalt-EDTA and ytterbium acetate as liquid and solid phase markers respectively. Purines were used as the bacterial marker (Zinn and Owens, 1986). Degradability of the feeds was measured in 3 other cows using the small bag technique described by De Boer et al. (1987).

Effect of feeding hay vs. silages of various types to dairy cows on feed intake, milk composition and coagulation properties

2020 ◽  
Vol 87 (3) ◽  
pp. 334-340
Author(s):  
Elisa Manzocchi ◽  
Werner Hengartner ◽  
Michael Kreuzer ◽  
Katrin Giller
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Feed Intake ◽  
Milk Fat ◽  
Fixed Effects ◽  
Experimental Period ◽  
Milk Composition ◽  
Processing Technology ◽  
Maize Silage ◽  
Rennet Coagulation

AbstractThis research paper addresses the hypotheses (1) that milk produced from hay-fed cows differs from that of silage-fed cows and (2) that silage type has an important impact, too. Four diets differing in forage type but with equal estimated milk production potential and a forage:concentrate ratio of 0.85 : 0.15 were compared regarding their effect on feed intake, milk yield and milk properties. The forages tested were hay, grass silage, conventional short-chopped and long-chopped maize silage subjected to a novel processing technology (Shredlage®). Twenty-four dairy cows were fed two of the four diets in two consecutive runs in an incomplete (4 × 2) Latin-square design (n = 12 per diet). Each experimental period lasted 22 d, with 12 d of adaptation and 10 d of sampling. During sampling, feed intake and milk yield were recorded daily, milk composition and coagulation properties were determined four times. The composition of the diet ingredients was analysed weekly. Data were analysed with a mixed model considering feed, period and their interaction as fixed effects. Stage of lactation, milk yield and milk composition from the pre-experimental period were used as covariates in the model. Dry matter intake was lower with the long-chopped processed maize silage compared to the other three groups. There were some diet differences in intakes of net energy for lactation and absorbable protein in the duodenum, but this did not result in changes in milk yield. The milk fat content was higher with the grassland-based diets compared to the maize silage diets. No treatment effect on milk acidity and rennet coagulation properties was observed. In conclusion, there were no indications for specific physico-chemical properties of milk from a hay-based diet, and maize processing technology was not of large effect either. Future investigations should focus on sensory differentiation of the milk produced with different forages.

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