scholarly journals Activities of N-acetyl-β-d-glucosaminidase and glutathione peroxidase in bovine colostrum and milk

2010 ◽  
Vol 55 (No. 11) ◽  
pp. 488-495 ◽  
Author(s):  
M. Kankofer ◽  
E. Albera ◽  
M. Różańska-Boczula

Parturition and post partum period are susceptible for antioxidative/oxidative imbalance as well as inflammatory processes related to either uterus or mammary gland. Fifteen Holstein-Friesian cows were used to examine the relationship between the antioxidant enzyme glutathione peroxidase (GSH-Px) and inflammatory enzyme N-acetyl-glucosaminidase (NAGase) in colostrum and milk during 12 days after parturition. Samples from each udder quarter were collected immediately after parturition, after 24 h and 48 h as well as after 6 and 12 days post partum. The activity of GSH-Px was measured spectrophotometrically and NAGase spectrofluorimetrically. NAGase activity generally remained constant during the examined period confirming no inflammation and healthy udder. Moreover, no significant differences in the activity among quarters were detected. The activity of GSH-Px rose significantly (P < 0.05) in the examined period suggesting the dynamic balance of antioxidative defence. No differences among quarters confirmed that in healthy quarters metabolic processes are on a similar level but positive correlations between the antioxidative and inflammatory enzyme in quarters and with regard to time may suggest a possible relationship during inflammation.

2010 ◽  
Vol 55 (No. 1) ◽  
pp. 488-495
Author(s):  
M. KANKOFER ◽  
E. ALBERA ◽  
M. RÓŻAŃSKA-BOCZULA

Parturition and post partum period are susceptible for antioxidative/oxidative imbalance as well as inflammatory processes related to either uterus or mammary gland. Fifteen Holstein-Friesian cows were used to examine the relationship between the antioxidant enzyme glutathione peroxidase (GSH-Px) and inflammatory enzyme N-acetyl-glucosaminidase (NAGase) in colostrum and milk during 12 days after parturition. Samples from each udder quarter were collected immediately after parturition, after 24 h and 48 h as well as after 6 and 12 days post partum. The activity of GSH-Px was measured spectrophotometrically and NAGase spectrofluorimetrically. NAGase activity generally remained constant during the examined period confirming no inflammation and healthy udder. Moreover, no significant differences in the activity among quarters were detected. The activity of GSH-Px rose significantly (P < 0.05) in the examined period suggesting the dynamic balance of antioxidative defence. No differences among quarters confirmed that in healthy quarters metabolic processes are on a similar level but positive correlations between the antioxidative and inflammatory enzyme in quarters and with regard to time may suggest a possible relationship during inflammation.


Author(s):  
Bartosz Wilczyński ◽  
Jakub Hinca ◽  
Daniel Ślęzak ◽  
Katarzyna Zorena

Rugby is a demanding contact sport. In light of research, poor balance, reduced jumping ability, muscle strength, and incorrect landing patterns might contribute to the increased risk of injury in athletes. Investigating the relationship between tests assessing these abilities might not only allow for the skillful programming of preventive training but also helps in assessing the risk of injury to athletes. Thus, the main purpose of this study was to investigate the relationship between dynamic balance, vertical and horizontal jumps, and jump-landings movement patterns. Thirty-one healthy amateur adolescent rugby players (age: 14.3 ± 1.6 years, height 171.4 ± 9.7 cm, body mass 80 ± 26 kg) participated in the study. Data were collected by the Y-balance Test (YBT), Counter Movement Jump (CMJ), Single Leg Hop for Distance (SLHD), and Landing Error Score System (LESS). Significant positive correlations were found between SLHD both legs (SLHDb) and YBT Composite both legs (COMb) (r = 0.51, p = 0.0037) and between SLHDb and CMJ (r = 0.72, p < 0.0001). A relationship was also observed between the CMJ and YBT COMb test (r = 0.51, p = 0.006). Moderate positive correlations were found between the dominant legs in SLHD and the posterolateral (r = 0.40, p = 0.027), posteromedial (r = 0.43, p = 0.014), and composite (r = 0.48, p = 0.006) directions of the YBT. These results indicate that variables that are dependent on each other can support in the assessment of injury-risk and in enhancing sports performance of young athletes.


2001 ◽  
Vol 26 (2) ◽  
pp. 467-470
Author(s):  
J.F. Cox ◽  
F. Saravia ◽  
O. Torrealba ◽  
A. Zavala ◽  
A. Lobos

AbstractControlled breeding schemes for oestrous detection constitutes a proactive technical response that balances the infrastructural requirement for a profitable dairy operation and the demands for optimal animal performance. The present study compared (a) the reproductive response of a treatment based on a short vs longer-acting PGF2α analogue (tiaprost vs luprostiol), and (b) the reproductive response after a treatment of GnRH-PGF2α vs PGF2α alone for synchronizing dairy cows. Holstein-Friesian cows averaging 9000 kg milk/lactation and fed according to their requirements were used in the study. Cows were cyclic, at least 60 days post partum and were clinically sound before being considered for the experiments. In Experiment 1, animals were synchronised using an i.m. injection of either 15 mg of luprostiol or 0.75 mg of tiaprost, based on ultrasonic diagnosis of a corpus luteum. Animals were inseminated at observed oestrus. In Experiment 2, cows were synchronised, at random, by either an injection of 10pg ofbuserelin (day 0) followed by 0.75 mg of tiaprost at day 7 (GnRH-PGF2α) orjust 0.75 mg of tiaprost (PGF2α). For both treatments only cows with an ultrasonically detected corpus luteum were treated. Animals were inseminated at oestrus. At the time of treatment and again 3 days later, milk samples were collected and assayed for progesterone by RIA. Cows with progesterone concentrations >1 ng/ml were considered to have corpus luteum. Luteolysis was considered to have occurred when concentrations of progesterone were > 1 ng/ml at day 0 and <0.8 ng/ml at day 3. In Experiment 1, both analogues gave similar results in terms of induced luteolysis [luprostiol: 36/39 (92.3%) vs tiaprost: 36/41 (87.8%)], oestrous detection efficiency [luprostiol: 26/36 (72.2%) vs tiaprost: 30/36 (83.3%], oestrous distribution [day 2, 3 and 4, respectively: luprostiol: 26.9%, 50.0%, 19.2% vs tiaprost: 36.7%, 50.0%, 13.3%], and conception rates [luprostiol: 12/25 (48.0%) vs tiaprost: 14/28 (50.0%); P>0.05]. In Experiment 2, oestrous detection efficiency, interval to oestrus and conception rate were similar between treatments [97/149 (65.1%), 71.1 h, 43/95 (45.3%) for PGF2α vs 130/188 (69.1%), 68.2h, 65/126 (51.6%) for GnRH-PGF2α, respectively]. However the oestrous distribution was more concentrated in GnRH-PGF2α treated animals (P<0.01).


2005 ◽  
Vol 2005 ◽  
pp. 53-53
Author(s):  
E. Wall ◽  
I. M. S. White ◽  
M. P. Coffey ◽  
S. Brotherstone

Cattle breeders, farmers and vets believe that the decline in fertility seen in recent years can be partially attributed to changes in rump angle with selection being for more angular cows. This suggests that animals with pin bones that sit above the hip bones (high rump angle) will have poorer fertility. Few studies have shown a significant relationship between fertility and rump traits. This study investigates the popular belief that high rump angle equates to poor fertility by examining the genetic and phenotypic correlation between rump angle and fertility traits. The relationship between rump angle and fertility was also examined to see if there was an intermediate optimum or threshold of rump angle for good/bad fertility. The correlation of other type traits (udder and composite traits) with fertility was also examined to see if they had potential to add information to the estimation of fertility breeding values.


1999 ◽  
Vol 68 (3) ◽  
pp. 527-532 ◽  
Author(s):  
A. O. Darwash ◽  
G. L. Ward ◽  
G. E. Lamming ◽  
J. A. Woolliams

AbstractIn order to investigate the rationale of manipulating post-ovulation progesterone (P4) concentrations, luteal activity was measured in 10 Holstein-Friesian cows treated with a progesterone-releasing device (CIDR, 1·9 g P4) inserted on the morning of day 2 post-oestrus for a period of 7 days and compared with 10 untreated control animals. Milk samples were collected daily during afternoon milking from 7 days pre- to 24 days post-oestrus and P4 concentrations were measured using enzyme-linked immuno-sorbent assay (ELISA). The milk P4 profiles were used to assess the effects of early post-oestrus P4 elevation on five intervals of the luteal phase. In the treated (T) and control (C) animals, the mean interval from oestrus to P4 rise (≥3 μg/l), was 2·38 (s.e. 0·18) and 4·90 (s.e. 0·28) days, respectively, (P < 0·01). The mean interval from oestrus to the attainment of peak P4 concentrations was not significantly different (P > 0·05) between the T (14·75 (s.e. 1-62) days) and С (14·30 (s.e. 0·70) days) animals, with cows in the T group showing a more variable (F7 9 = 4-30, P < 0·05) interval to the occurrence of the peak. The interval from peak P4 to corpus luteum (CL) regression (when P4 fell below ≥3 μg/l) was not significantly different between the T (4·13 (s.e. 1·30 days) and С (5·60 (s.e. 0·88) days) groups. Furthermore, CIDR insertion did not alter luteal phase length, number of days with P4 concentrations ≥3 μg/l (T = 16·50 (s.e. 0·80) v.C = 15·00 (s.e. 0·47) days) or the interval from oestrus to CL regression (T = 18·88 (s.e. 0·79) v.C = 19·90 (s.e. 0·41) days). We conclude that treatment with CIDR 2 days after oestrus successfully increased the availability of P4 to cows without significantly affecting luteal phase characteristics.


2001 ◽  
Vol 72 (3) ◽  
pp. 539-546 ◽  
Author(s):  
A. O. Darwash ◽  
G. E. Lamming ◽  
M. D. Royal

AbstractThe objective of the present study was to evaluate the efficacy of a prostaglandin F2α(PGF2α) and progesterone (P4) treatment in initiating oestrus and ovulation post partum (PP) in Holstein-Friesian cows. Using four herds, the treatment protocol consisted of a single intra-muscular injection of PGF2α(Estrumate) between days 12 to 14 PP followed 48 h later by progesterone treatment via intra-vaginal CIDR insertion for a period of 7 days. Milk samples for progesterone determination were collected three times weekly from 7 to 65 days PP. The ovarian activity and reproductive performance of treated (T, no. = 153) animals and untreated control cows (C, no. = 315) were compared. Treatment was effective (P< 0·001) in reducing the mean interval to PP commencement of luteal activity from 29·62 (s.e. 0·82) days to 22·09 (s.e. 0·70) days. The mean interval to first PP oestrus in the T animals was significantly reduced (P< 0·001) from 55·62 (s.e. 1·58) days to 44·91 (s.e. 1·44) days and the incidence of silent ovulation in cycles between days 21 to 65 PP was reduced (P< 0·001) from 56·28% to 42·27%. In two herds under one management regime and with a similar block-calving pattern (no. = 280 animals), the treatment protocol was beneficial to the overall reproductive performance as there was a significant shortening in the mean interval to first PP service (75·82 (s.e. 1·93) v. 80·86 (s.e. 1·32) days) and in the interval to PP conception (83·07 (s.e. 2·49) v. 88·90 (s.e. 1·95) days), bothP< 0·05.


1993 ◽  
Vol 56 (2) ◽  
pp. 281-283 ◽  
Author(s):  
M. J. Gibb ◽  
W. E. Ivings

The relationships between body composition, live weight (LW) and subjective condition score (CS) were examined in an experiment in which 54, second to fourth parity, lactating autumn-calving Holstein-Friesian dairy cows were slaughtered at 0, 2, 5, 8, 11, 14, 19, 24 and 29 weeks post partum. Multiple regression analyses showed the weight of fat and the quantity of energy within the body were significantly correlated with both LW and CS. The weight of crude protein showed a linear correlation with LW. These results are discussed and compared with previously published investigations with non-lactating dairy cows.


2013 ◽  
Vol 63 (5-6) ◽  
pp. 487-498 ◽  
Author(s):  
Milan Maletic ◽  
Slobodanka Kanjac ◽  
N. Djelic ◽  
Nada Lakic ◽  
M. Pavlovic ◽  
...  

2017 ◽  
Vol 16 (4) ◽  
pp. 33-38
Author(s):  
Weronika Kulpa ◽  
◽  
Anna Sawa ◽  
Małgorzata Jankowska ◽  
◽  
...  

2019 ◽  
Vol 102 (2) ◽  
pp. 1702-1711
Author(s):  
M. Gobikrushanth ◽  
D.C. Purfield ◽  
J. Kenneally ◽  
R.C. Doyle ◽  
S.A. Holden ◽  
...  

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