scholarly journals DNA microsatellite analysis of genetic diversity among Chinese indigenous laying-type ducks (Anas platyrhynchos)

2009 ◽  
Vol 54 (No. 3) ◽  
pp. 128-135 ◽  
Author(s):  
Y. Su ◽  
G.H. Chen

The genetic polymorphisms of 17 microsatellites were investigated in four indigenous laying-type duck breeds in China. The average number of alleles (<I>Na</I>) and average rates of homozygotes of each breed were counted. Accordingly, allele frequencies of the 17 microsatellites, polymorphism information content (<I>PIC</I>), mean heterozygosity (<I>H</I>) and genetic distances (<I>Ds</I>) were also calculated. Moreover, dendrograms using UPGMA and the neighbour-joining method were produced. The four breeds have a high average <I>PIC</I> (0.643) and <I>H</I> (0.682). <I>Ds</I> are between 0.514 and 0.662, the gene differentiation among the four breeds is 14.4%.

2000 ◽  
Vol 9 (4) ◽  
pp. 421-431 ◽  
Author(s):  
Lisette Waits ◽  
Pierre Taberlet ◽  
Jon E. Swenson ◽  
Finn Sandegren ◽  
Robert Franzen

2008 ◽  
Vol 53 (No. 8) ◽  
pp. 315-319 ◽  
Author(s):  
J.Y. Li ◽  
H. Chen ◽  
X.Y. Lan ◽  
X.J. Kong ◽  
L.J. Min

The genetic diversity was studied using microsatellite DNA markers in Laoshan dairy goat (LS), Xinong Saanen dairy goat (SN), Guanzhong dairy goat (GZ), Banjiao goat (BJ) and Guizhou white goat (GW). Within the nine polymorphic loci, allele frequencies, number of effective alleles (Ne), heterozygosity (He), polymorphism information content (PIC), genetic identity (I) and Nei’s standard genetic distance (D) were calculated, and UPGMA phylogenetic tree was constructed based on allele frequencies. The average number of alleles was 9.4, ranging from four to eleven at the nine assessed loci. The average values of Ne, He, PIC of all loci were 4.716, 0.765, 0.732, respectively. The GZ population showed the highest variability (PIC = 0.78, He = 0.80). There was a relatively high level of genetic diversity in these goat breeds. A UPGMA diagram, based on Nei’s standard genetic distances, yielded relationships between populations that agreed with what is known about their origin, history and geographical distribution.


1998 ◽  
Vol 43 (4-5) ◽  
pp. 281-288 ◽  
Author(s):  
Benoît Goossens ◽  
Laurent Graziani ◽  
Lisette P. Waits ◽  
Etienne Farand ◽  
Séverine Magnolon ◽  
...  

Nature ◽  
10.1038/28521 ◽  
1998 ◽  
Vol 394 (6691) ◽  
pp. 329-329 ◽  
Author(s):  
David Ashworth ◽  
Matthew Bishop ◽  
Keith Campbell ◽  
Alan Colman ◽  
Alex Kind ◽  
...  

2013 ◽  
Vol 61 (5) ◽  
pp. 357 ◽  
Author(s):  
Anas M. Khanshour ◽  
Rytis Juras ◽  
E. Gus Cothran

The Waler horse breed is an integral part of Australian history. The purposes of this study were to analyse the genetic variability in Waler horses from Australia and to investigate genetic relationships with other horse breeds. We examined the genetic diversity of 70 Waler horses sampled from seven breeding stations in Australia. Also we analysed the relationships of these horses with 11 other horse breeds. Analysis of the genetic structure was carried out using 15 microsatellite loci, genetic distances, AMOVA, factorial correspondence analysis and a Bayesian method. We found that the genetic diversity in the Waler was greater than the domestic horse mean and exceeded that of all endangered horse breeds. Our findings also revealed moderate population subdivision rather than inbreeding. All genetic similarity measures indicated that the Thoroughbred might be a key ancestor to the Waler. This study indicates that there is no immediate concern for loss of variation in Waler horses. Also, there clearly has been a strong input from the Thoroughbred into the Waler horse breed. However, the genetic evidence suggests that this input was not just direct but also came through other types of horses with a Thoroughbred cross background.


2015 ◽  
Vol 15 (1) ◽  
pp. 64-71 ◽  
Author(s):  
Khaled Mirzaei ◽  
Ghader Mirzaghaderi

The genetic diversity of 39 Iranian black cumin (Nigella sativa L.) landraces was analysed using 14 polymorphic Start Codon Targeted (SCoT) markers. A total of 106 bands ranging from 3 (for SCoT70) to 13 (for SCoT23) were observed. Of them, 33 (31%) bands were polymorphic with a mean of 1.65 bands per primer. Polymorphism information content (PIC) per primer ranged from 0.035 (for SCoT12) to 0.133 (for SCoT70), with an average of 0.078. Besides PIC, Simpson's diversity (D) index was also calculated for each SCoT marker as an indication of discrimination power across population. The D index was used to adjust the PIC of the SCoT markers. As the adjusted PIC (PICD= PIC × D) was calculated based on both the PIC and the rate of band dispersion, this reflected the informativeness of a dominant marker more precisely than PIC. Genetic relationships were estimated using Jaccard's similarity coefficient-generated values between different pairs of genotypes that varied from 80 to 97% with an average of 88%. These coefficients were applied to construct a dendrogram using the UPGMA algorithm. A high genetic similarity was observed that might be due to the fact that N. sativa is a self-pollinated plant not originated from Iran and might have been imported from the Mediterranean regions.


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