scholarly journals Effects of dietary manganese on antioxidant status, biochemical parameters and thickness of intestinal mucus in laying hens

2019 ◽  
Vol 64 (No. 3) ◽  
pp. 99-106
Author(s):  
Elena Piešová ◽  
Zita Faixová ◽  
Zuzana Maková ◽  
Katarína Venglovská ◽  
Ľubomíra Grešáková ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Antioxidant Status ◽  
Laying Hens ◽  
Protein Hydrolysate ◽  
Plasma Cholesterol ◽  
Total Antioxidant Status ◽  
Antioxidant Potential ◽  
Cholesterol Concentration ◽  
Control Group ◽  
Intestinal Mucus

The objective of this study was to investigate the effects of different sources of manganese (Mn) on the antioxidant status, some biochemical parameters and thickness of intestinal mucus in laying hens. Ninety-six 20-week-old hens of the Lohman Brown strain were randomly divided into four groups; each group was replicated six times with four hens per replicate. Hens in the control group were fed the basal diet (BD) with natural background Mn level of 45.5 mg/kg feed. For the three experimental groups, the BD was supplemented with 120 mg Mn/kg either from Mn-sulphate or Mn-chelate of protein hydrolysate (Mn-Bioplex) or Mn-chelate of glycine hydrate (Mn-Glycinoplex). After eight weeks of dietary treatment, a tendency towards increasing activity of superoxide dismutase (SOD) in the erythrocytes was observed in all experimental groups. The results showed that inorganic Mn source increased the glutathione peroxidase activity in the hens’ blood (P < 0.05). The intake of Mn-Bioplex resulted in significantly increased total antioxidant status in the plasma of hens in the group (P < 0.05) compared to the control group. The plasma biological antioxidant potential was increased in the treatment groups supplemented with Mn-sulphate (P < 0.01) and Mn-Bioplex (P < 0.05). The total cholesterol concentration was decreased (P < 0.05) in the group fed the diet supplemented with Mn-Glycinoplex, as compared with the control. Moreover, the mucus layer thickness in the jejunum was reduced in the groups of birds fed with the organic form of Mn (P < 0.001, P < 0.05), as well as with the inorganic form of Mn (P < 0.001). These results indicate that the addition of dietary Mn was effective in improving the biological antioxidant potential, decreasing total plasma cholesterol and reducing the thickness of adherent intestinal mucus gel in the jejunum of laying hens.

Effect of Piperine on the Pharmacokinetics and Pharmacodynamics of Glimepiride in Normal and Streptozotocin - Induced Diabetic Rats

2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Ciddi Veeresham ◽  
Samala Sujatha ◽  
Thatipamula Sandya Rani
Keyword(s):  
Biochemical Parameters ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Diabetic Rats ◽  
Pharmacokinetic Parameters ◽  
Control Group ◽  
Diabetic Patients ◽  
Significant Protection ◽  
Pharmacokinetics And Pharmacodynamics ◽  
Total Antioxidant

The effect of piperine on the pharmacokinetics and pharmacodynamics of glimepiride in normal as well as diabetic rats was studied. In normal and streptozotocin induced diabetic rats the combination of glimepiride with piperine increased all the pharmacokinetic parameters, such as Cmax, AUC0-n, AUCtotal, t½, and MRT, and decreased the clearance, Vd, markedly as compared with the control group. In pharmacodynamic studies, the combination of glimepiride with piperine provided significant protection against the diabetes induced alterations in the biochemical parameters. In addition, the combination of glimepiride with piperine also improved the total antioxidant status significantly in diabetic rats compared with piperine and glimepiride treated groups. The results revealed that a combination of glimepiride with piperine led to the enhancement of the bioavailability of glimepiride by inhibiting the CYP2C9 enzyme, which suggested that piperine might be beneficial as an adjuvant to glimepiride in a proper dose, in diabetic patients.

Antioxidant status of blood in patients with vibration disease

2019 ◽  
pp. 978-982 ◽  
Author(s):  
N. N. Malyutina ◽  
A. F. Bolotova ◽  
R. B. Eremeev ◽  
A. Zh. Gilmanov ◽  
D. Yu. Sosnin
Keyword(s):  
Antioxidant Activity ◽  
Blood Serum ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Main Group ◽  
Biologically Active ◽  
Antioxidant Systems ◽  
Control Group ◽  
Vibration Disease ◽  
Total Antioxidant

Introduction. The overwhelming number of publications contains only data on the content of individual antioxidants, but not on the overall antioxidant activity of the blood in patients with vibration disease.The aim of the study was to determine the total antioxidant activity of blood serum in patients with vibration disease.Materials and methods. Th e main group consisted of 30 people diagnosed with “Vibration disease” of 1 degree (n=21) and 2 degrees (n=9). Th e control group consisted of 30 clinically healthy men, comparable in age with the main group (p=0.66). Th e total activity of antioxidant systems of blood plasma was evaluated photometrically using the test system “Total antioxidant status-Novo” (“Vector-best”, Russia).Results. The indicator of the total antioxidant status (TAS) was 1,038±0.232 mmol/l in the examined main group, against 1,456±0.225 mmol/l in the examined control group (p<0.000001). Th e coefficient of variation (CV) in patients with vibration disease was 22.35%, 1.45 times higher than in the control group (15.45%). In the main group there was a positive correlation between age and TAS (R=0.525), in the control group there was no such relationship (R=0.095). Th e degree of decrease depended on the severity of vibration disease.Conclusions. 1. The development of vibration disease is accompanied by a decrease in the antioxidant status of blood serum. 2. Th e degree of decrease in the antioxidant status of blood serum correlates with the severity of vibration disease. 3. Reduction of TAS can serve as a pathogenetic justification of the need to include drugs and/or biologically active additives with antioxidant activity in therapy

scholarly journals Effects of Dietary Inclusion of Dry Hydrastis canadensis on Laying Performance, Egg Quality, Serum Biochemical Parameters and Cecal Microbiota in Laying Hens

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1381
Author(s):  
Tzuen-Rong J Tzeng ◽  
Tzu-Yu Liu ◽  
Chiao-Wei Lin ◽  
Pei-En Chang ◽  
Pei-Xin Liao ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Egg Production ◽  
Laying Hens ◽  
Basal Diet ◽  
Response To Treatment ◽  
Control Group ◽  
Hydrastis Canadensis ◽  
Laying Performance ◽  
Cecal Microbiota ◽  
Dietary Treatments

Alternative growth promoters are able to not only effectively replace the traditional use of antibiotics but also provide additional health benefits for livestock and reduce food safety concerns. This study investigated the effects of dry Hydrastis canadensis on the laying performance and fecal microbial community of laying hens. Twenty-four Lohmann (LSL, white layer strain) hens were reared from 40 to 48 weeks of age and randomly allotted to four dietary treatments (six birds/treatment). The dietary treatments comprised a basal diet with no treatment as control, a basal diet plus 0.6% powder of dry Hydrastis canadensis roots (R) or leaves (L), and a basal diet plus 0.6% powder of a mixture of dry Hydrastis canadensis roots and leaves (1:1, LR). No mortality was observed in the whole experimental period. The results indicated that albumen height in the LR group was significantly greater than that in the control group. The diet supplemented with Hydrastis canadensis had no significant effects on egg production rate, egg weight, eggshell strength, eggshell thickness, Haugh unit, or yolk height during the whole experimental phase. However, principal coordinate analysis, comparative heat map analysis, and cluster dendrogram analysis of cecal microbiota showed distinct clusters among the groups treated with Hydrastis canadensis and the control group. Regarding blood biochemical parameters, serum cholesterol levels were significantly lower in all Hydrastis canadensis-treated groups compared with those in the control group. Moreover, serum low-density lipoprotein levels were lower in hens supplemented with the leaf of Hydrastis canadensis. The abundances of the phyla Fusobacteria and Kiritimatiellaeota were increased (p < 0.05) in laying hens fed with 0.6% Hydrastis canadensis leaves, whereas the abundance of the phylum Firmicutes in cecum digesta decreased in response to treatment with Hydrastis canadensis roots and leaves. The relative abundance of the Fusobacterium genus was higher in the LR group compared with that in the control. On the contrary, we found a different trend in the Synergistes genus. The potential influences of these microbiota on the performance of laying hens were discussed. The results demonstrate that Hydrastis canadensis can improve the egg albumen height and modulate the cecum digesta microbiota composition of laying hens.

scholarly journals Dietary Plant Extracts Improve the Antioxidant Reserves in Weaned Piglets

Antioxidants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 702
Author(s):  
Carlo Corino ◽  
Michel Prost ◽  
Barbara Pizzi ◽  
Raffaella Rossi
Keyword(s):  
Antioxidant Activity ◽  
Plant Extracts ◽  
Whole Blood ◽  
Antioxidant Status ◽  
Biological Activities ◽  
Total Antioxidant Status ◽  
Dietary Treatment ◽  
Control Group ◽  
Commercial Diet ◽  
Total Antioxidant

Reducing the use of antibiotics in livestock in order to contain antibiotic resistance and studying natural substance additives are key to sustainability. Among the various biological activities of plant extracts, antioxidant activity plays an important role. The present study assesses the total antioxidant activity and antioxidant reserves using the Kit Radicaux Libres test (KRL™ Kirial International, Couternon, France). One hundred and sixty piglets (Topics × Tempo) weaned at 28 days of age were divided into four dietary treatment groups that were fed a commercial diet (the control group, C); 500 mg/kg Boswellia extract (BOS); 200 and 50 mg/kg Uncaria and Tanacetum extracts (UT) respectively; and 225 mg/kg of an antioxidant plant extract mixture (AOX). The blood antioxidant activity of the piglets was measured using the KRL test and the reserves were analyzed on whole blood samples after hydrolysis with glucosidase, sulfatase and glucuronidase. No significant differences were observed in growth performance. The delta KRL values of the whole blood showed a significantly higher total antioxidant status of the piglets from the BOS and AOX groups than the UT and C groups (+30.7 BOS; +27.7 AOX vs. +17.81 UT +13.30 C; p = 0.002) between 18 and 28 days post-weaning. The delta KRL values of red blood cells (RBCs) showed a significantly higher total antioxidant status of the piglets from the AOX groups than the UT and BOS groups (+22.2 AOX; vs. +9.90 UT +9.4 BOS; p = 0.016) between the two sampling times. Reserves of UT and AOX were higher than C and BOS for all enzymes, glucosides, sulphates, and glucuronides. The biological KRL test proved to be an extremely sensitive tool to evaluate the piglets’ antioxidant status. Determining the antioxidant reserve also provides a better understanding of the real antioxidant status of pigs.

scholarly journals Serum Homocysteine and Reactive Species Levels in Fragility Fractures of the Pelvis

2019 ◽  
Vol 70 (9) ◽  
pp. 3216-3219 ◽  
Author(s):  
Alexandru Filip ◽  
Oana Viola Badulescu ◽  
Paul Dan Sirbu ◽  
Elena Cojocaru ◽  
Nina Filip ◽  
...  
Keyword(s):  
Bone Quality ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Fragility Fractures ◽  
Reactive Species ◽  
Control Group ◽  
Serum Homocysteine ◽  
Total Antioxidant ◽  
The Relationship

Although there are studies showing the link between homocysteine and bone quality, the relationship remains controversial. The purpose of the study was to investigate homocysteine levels in patients with fragility fractures of the pelvis and their association with serum Total Antioxidant Status.The study included a group of 60 patients, aged ]65years, with pelvic fragility fractures and osteoporosis admitted in our trauma department between January 2015 and January 2018 and a control group of 30 patients, aged ]65years without fragility fractures or osteoporosis.The total antioxidant status (TAS) and homocysteine (Hcy) concentrations were determined in serum of all the patients. TAS levels had a significantly lower values in the group with pelvic fragility fractures compared with the control group (p[0.001).We noticed a significant higher mean of Hcy levels (p[0.001) in patients with pelvic fragility fractures compared with the control group. As conclusion this study shows that patients with fragility fractures of the pelvis have moderately elevated levels of homocysteine and reduced serum Total Antioxidant Status.

scholarly journals Relationship between diet, oxidative stress, and inflammation in ankylosing spondylitis

2021 ◽  
Author(s):  
Kübra Tel Adıgüzel ◽  
Fatma Gül Yurdakul ◽  
Nilgün Seremet Kürklü ◽  
Evren Yaşar ◽  
Hatice Bodur
Keyword(s):  
Oxidative Stress ◽  
Fatty Acid ◽  
Ankylosing Spondylitis ◽  
Vitamin C ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Stress Index ◽  
Control Group ◽  
Dietary Record ◽  
Significant Difference

Objectives: This study aims to investigate the relationship between disease activity, dietary phytochemical index (DPI), and serum total oxidant status (TOS) and total antioxidant status (TAS) in patients with ankylosing spondylitis (AS). Patients and methods: Between August 2020 and January 2021, a total of 37 patients (23 males, 14 females; mean age: 39.3±9.4 years; range, 21 to 61 years) with AS and 36 age-, sex-, and body mass index-matched healthy individuals (24 males, 12 females; mean age: 37.9±8.9 years; range, 20 to 60 years) were included. Serum TAS (μmoLTroloxEq/L) and TOS (μmoL H2O2Eq/L) measurements were performed and the oxidative stress index (OSI) was calculated. Dietary evaluation was made from a one-day dietary record and DPI was calculated. Results: Serum TAS level in AS patients was significantly lower than the healthy group (p=0.003). Serum TOS level was similar in both groups. The OSI of patients was significantly higher than the controls (p=0.035). The mean DPI, polyunsaturated fatty acid, n-3 fatty acid, and vitamin C intake of patients were significantly lower than controls (p=0.042, p=0.033, and p=0.022, respectively). A moderate positive correlation was found between the TAS level and DPI of the control group (r=0.352, p=0.035). According to medications, no significant difference was seen between the groups in terms of patients’ characteristics, DPI, and laboratory tests and there was no correlation between DPI, TAS, TOS, and OSI. Conclusion: Lower DPI and lower n-3 fatty acid and vitamin C intake in patient group demonstrated that patients with AS should pay more attention to their diet to increase serum antioxidant status.

scholarly journals Effects of feed supplementation with manganese from its different sources on performance and egg parameters of laying hens

2014 ◽  
Vol 59 (No. 4) ◽  
pp. 147-155 ◽  
Author(s):  
K. Venglovská ◽  
Ľ. Grešáková ◽  
I. Plachá ◽  
M. Ryzner ◽  
K. Čobanová
Keyword(s):  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Protein Hydrolysate ◽  
Basal Diet ◽  
Egg Yolk ◽  
Control Group ◽  
Feed Supplementation ◽  
Positive Effects ◽  
Dietary Treatments

The objective of this study was to compare the effects of feed supplementation of laying hens with manganese from its inorganic and organic sources on performance and some parameters of egg quality. Ninety-six hens at 20 weeks of age were randomly allocated to 4 dietary treatments, each consisting of 6 replicates (4 birds per replicate). The control group was fed unsupplemented basal diet (BD) with only natural background Mn level of 46.4 mg/kg feed. For the three experimental treatments, the BD was supplemented with 120 mg Mn/kg either from Mn-sulphate or Mn-chelate of protein hydrolysate (Mn-Pro) or Mn-chelate of glycine hydrate (Mn-Gly). After 8 weeks of dietary treatments the egg production, egg weight, feed intake, and feed efficiency were not affected by dietary treatments. Regardless of the sources, Mn supplementation to feed resulted in significantly decreased percentages of cracked eggs compared to the unsupplemented control group. The thickness, weight, proportion, and index of eggshell were significantly elevated in all groups supplemented with Mn. The intake of Mn-Gly resulted in considerably increased Mn deposition in egg yolk compared to the control eggs. In the control and Mn-sulphate groups yolk malondialdehyde (MDA) started to increase after 20 and 30 days of egg storage respectively, whereas in eggs from hens given organic Mn-sources this parameter was not affected up to 40 days. Although there were no significant differences in MDA values between the treatments until 20 days of storage, the Mn-sulphate group showed significantly higher MDA concentration in yolks compared to the control group after 30 days of storage. These results demonstrate that supplementation of hens&rsquo; diet with Mn has positive effects on eggshell quality. Feed supplementation with Mn from organic sources appears to be more effective in preventing yolk lipid oxidation during cold storage of eggs than that from Mn-sulphate. &nbsp;

​A Study on Alteration in Serum Biochemical Parameters in Colibacillosis Affected Diarrhoeic Cattle Calves

2021 ◽  
Author(s):  
T.C. Nayak ◽  
A.P. Singh ◽  
A. Chahar ◽  
Savita . ◽  
R. Yadav ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Total Antioxidant Status ◽  
Stress Index ◽  
Control Group ◽  
Serum Biochemical Parameters ◽  
Serum Samples ◽  
Clinically Normal ◽  
Serum Biochemical ◽  
Result Analysis ◽  
G Ratio

Background: The objective of the present study was to evaluate the changes in serum biochemical parameters in colibacillosis affected diarrhoeic cattle calves below one month of age. Methods: The study was undertaken at Veterinary Clinical Complex, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, Bikaner, Rajasthan, India, during the period from September 2018 to March 2019. A total of 32 calves affected with colibacillosis confirmed on the basis of Multiplex PCR (for presence of K99, Stx1 and eaeA genes of E. coli) and eight clinically normal healthy calves (as control) were taken to investigate the serum biochemical profiles. Result: Analysis of serum samples of calves affected with colibacillosis revealed significant increase in the values of total protein, albumin, globulin, A/G ratio, blood urea nitogen, creatinine, potassium, total oxidant status andoxidative stress index while there is a significant decrease in the values of glucose, sodium, chloride, bicarbonate, IgG, IgM and total antioxidant status. There were no significant changes observed in the values of ALP, AST and ALT in affected calves as compared to healthy control group of calves.

Oxidative stress increases in carbon monoxide poisoning patients

2010 ◽  
Vol 30 (2) ◽  
pp. 160-164 ◽  
Author(s):  
Havva Sahin Kavakli ◽  
Ozcan Erel ◽  
Orhan Delice ◽  
Gizem Gormez ◽  
Semra Isikoglu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Carbon Monoxide ◽  
Antioxidant Status ◽  
Carbon Monoxide Poisoning ◽  
Total Antioxidant Status ◽  
Control Group ◽  
Total Oxidant Status ◽  
Oxidative Stress Parameters ◽  
Total Antioxidant ◽  
Oxidant Status

Objective: Carbon monoxide poisoning (COP) is one of the important causes of morbidity and mortality in toxicological cases. In this study, we aimed to find out more about the pathophysiology of COP by investigating the effects of COP on oxidative stress parameters such as total oxidant status (TOS) and total antioxidant status (TAS). Methods: Eighty-eight patients admitted to the emergency department of our hospital with acute COP and 35 healthy adults as control group were included in this study. Blood samples were collected from all COP patients at the time of initial emergency department evaluation to determine the oxidative stress parameters. Then, serum levels of total antioxidant status and total oxidant status levels were measured. Results: A total of 88 patients poisoned by carbon monoxide (CO; mean age 37.1 ± 18.2 years; 54’% women) were enrolled. TOS and carboxyhemoglobin (COHb) levels in COP patients were increased when compared to control group (p = 0.001). TOS, oxidative stress index (OSI) and COHb levels in COP patients were significantly lower after the treatment. (respectively, p = 0.016; p = 0.023; p = 0.001). On the other hand, no statistical differences were observed in TAS levels of study and control group as well as there were no changes with treatment. Conclusion: Measurements of TOS, TAS and OSI levels may be useful markers to find out the pathophsiology of COP.

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