scholarly journals Characterisation of a PR-1: Luciferase transgenic line deployed to uncover novel defence-related Arabidopsis mutants by luciferase imaging

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 615-616
Author(s):  
A. Chini ◽  
S. Murray ◽  
J. Grant ◽  
C. Thomson ◽  
G. Loake
Keyword(s):  
Disease Resistance ◽  
Transgenic Line ◽  
Low Light ◽  
Arabidopsis Mutants ◽  
Signalling Network ◽  
Ethylmethane Sulfonate ◽  
Defence Signalling

In order to identify components of the defence signalling network that may contribute to the establishment of disease resistance, we generated a novel PR-1::Luciferase transgenic line which was deployed in an imaging based screen to uncover novel defence-related mutants. Approximately, 5000 ethylmethane sulfonate (EMS) lines and 30 000 activation tagged lines were generated and screened for enhanced LUC activity via ultra low light imaging.

scholarly journals Dominant‐negative interference with defence signalling by truncation mutations of the tomato Cf‐9 disease resistance gene

2006 ◽  
Vol 46 (3) ◽  
pp. 385-399 ◽  
Author(s):  
Claire L. Barker ◽  
Brett K. Baillie ◽  
Kim E. Hammond‐Kosack ◽  
Jonathan D. G. Jones ◽  
David A. Jones
Keyword(s):  
Disease Resistance ◽  
Resistance Gene ◽  
Dominant Negative ◽  
Disease Resistance Gene ◽  
Negative Interference ◽  
Defence Signalling ◽  
Dominant Negative Interference

scholarly journals Resistance Enhancement of Transgenic Tomato to Bacterial Pathogens by the Heterologous Expression of Sweet Pepper Ferredoxin-I Protein

2007 ◽  
Vol 97 (8) ◽  
pp. 900-906 ◽  
Author(s):  
Hsiang-En Huang ◽  
Chien-An Liu ◽  
Mei-Jiuan Lee ◽  
C.-George Kuo ◽  
Huei-Mei Chen ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Plant Disease Resistance ◽  
Transgenic Line ◽  
Bacterial Pathogens ◽  
Sweet Pepper ◽  
Transgenic Tomato ◽  
Root Tissue ◽  
Expression Levels ◽  
Transgenic Lines ◽  
Ferredoxin I

Expression of a foreign gene to enhance plant disease resistance to bacterial pathogens is a favorable strategy. It has been demonstrated that expressing sweet pepper ferredoxin-I protein (PFLP) in transgenic plants can enhance disease resistance to bacterial pathogens that infect leaf tissue. In this study, PFLP was applied to protect tomato (Lycopersicon esculentum cv. cherry Cln1558a) from the root-infecting pathogen, Ralstonia solanacearum. Independent R. solanacearum resistant T1 lines were selected and bred to produce homozygous T2 generations. Selected T2 transgenic lines 24-18-7 and 26-2-1a, which showed high expression levels of PFLP in root tissue, were resistant to disease caused by R. solanacearum. In contrast, the transgenic line 23-17-1b and nontransgenic tomato, which showed low expression levels of PFLP in root tissue, were not resistant to R. solanacearum infection. The expansion of R. solanacearum populations in stem tissue of transgenic tomato line 24-18-7 was limited compared with the nontransgenic tomato Cln1558a. Using a detached leaf assay, transgenic line 24-18-7 was also resistant to maceration caused by E. carotovora subsp. carotovora; however, resistance to E. carotovora subsp. carotovora was less apparent in transgenic lines 26-2-1a and 23-17-1b. These results demonstrate that PFLP is able to enhance disease resistance at different levels to bacterial pathogens in individual tissue of transgenic tomato.

scholarly journals Heterologous Expression of the Constitutive Disease Resistance 2 and 8 Genes from Poncirus trifoliata Restored the Hypersensitive Response and Resistance of Arabidopsis cdr1 Mutant to Bacterial Pathogen Pseudomonas syringae

Plants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 821
Author(s):  
Xiaobao Ying ◽  
Bryce Redfern ◽  
Frederick G. Gmitter ◽  
Zhanao Deng
Keyword(s):  
Disease Resistance ◽  
Pseudomonas Syringae ◽  
Hypersensitive Response ◽  
Plant Disease Resistance ◽  
Marker Gene ◽  
Bacterial Pathogen ◽  
The United States ◽  
Poncirus Trifoliata ◽  
Close Relative ◽  
Arabidopsis Mutants

Huanglongbing (HLB), also known as citrus greening, is the most destructive disease of citrus worldwide. In the United States, this disease is associated with a phloem-restricted bacterium, Candidatus Liberibacter asiaticus. Commercial citrus cultivars are susceptible to HLB, but Poncirus trifoliata, a close relative of Citrus, is highly tolerant of HLB. Isolating P. trifoliata gene(s) controlling its HLB tolerance followed by expressing the gene(s) in citrus is considered a potential cisgenic approach to engineering citrus for tolerance to HLB. Previous gene expression studies indicated that the constitutive disease resistance (CDR) genes in P. trifoliata (PtCDRs) may play a vital role in its HLB tolerance. This study was designed to use Arabidopsis mutants as a model system to confirm the function of PtCDRs in plant disease resistance. PtCDR2 and PtCDR8 were amplified from P. trifoliata cDNA and transferred into the Arabidopsis cdr1 mutant, whose resident CDR1 gene was disrupted by T-DNA insertion. The PtCDR2 and PtCDR8 transgenic Arabidopsis cdr1 mutant restored its hypersensitive response to the bacterial pathogen Pseudomonas syringae pv. tomato strain DC3000 (Pst DC3000) expressing avrRpt2. The defense marker gene PATHOGENESIS RELATED 1 (PR1) expressed at much higher levels in the PtCDR2 or PtCDR8 transgenic cdr1 mutant than in the non-transgenic cdr1 mutant with or without pathogen infection. Multiplication of Pst DC3000 bacteria in Arabidopsis was inhibited by the expression of PtCDR2 and PtCDR8. Our results showed that PtCDR2 and PtCDR8 were functional in Arabidopsis and played a positive role in disease resistance and demonstrated that Arabidopsis mutants can be a useful alternate system for screening Poncirus genes before making the time-consuming effort to transfer them into citrus, a perennial woody plant that is highly recalcitrant for Agrobacterium or biolistic-mediated transformation.

Constitutive disease resistance requires EDS1 in the Arabidopsis mutants cpr1 and cpr6 and is partially EDS1 -dependent in cpr5

2001 ◽  
Vol 26 (4) ◽  
pp. 409-420 ◽  
Author(s):  
Joseph D. Clarke ◽  
Nicole Aarts ◽  
Bart J. Feys ◽  
Xinnian Dong ◽  
Jane E. Parker
Keyword(s):  
Disease Resistance ◽  
Arabidopsis Mutants

Arabidopsis mutants simulating disease resistance response

Cell ◽  
1994 ◽  
Vol 77 (4) ◽  
pp. 565-577 ◽  
Author(s):  
Robert A. Dietrich ◽  
Terrence P. Delaney ◽  
Scott J. Uknes ◽  
Eric R. Ward ◽  
John A. Ryals ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Resistance Response ◽  
Arabidopsis Mutants

scholarly journals Understanding and Exploiting Post-Translational Modifications for Plant Disease Resistance

Biomolecules ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 1122
Author(s):  
Catherine Gough ◽  
Ari Sadanandom
Keyword(s):  
Disease Resistance ◽  
Immune Responses ◽  
Plant Disease Resistance ◽  
Plant Immunity ◽  
Dynamic Responses ◽  
Post Translational Modifications ◽  
Pathogen Effectors ◽  
Trade Offs ◽  
Defence Signalling ◽  
Fine Tune

Plants are constantly threatened by pathogens, so have evolved complex defence signalling networks to overcome pathogen attacks. Post-translational modifications (PTMs) are fundamental to plant immunity, allowing rapid and dynamic responses at the appropriate time. PTM regulation is essential; pathogen effectors often disrupt PTMs in an attempt to evade immune responses. Here, we cover the mechanisms of disease resistance to pathogens, and how growth is balanced with defence, with a focus on the essential roles of PTMs. Alteration of defence-related PTMs has the potential to fine-tune molecular interactions to produce disease-resistant crops, without trade-offs in growth and fitness.

scholarly journals In-Silico Characterization and Expression Analysis of NB-ARC Genes in Response to Erwinia mallotivora in Carica papaya

2021 ◽  
Vol 50 (9) ◽  
pp. 2591-2602
Author(s):  
Nur Syazana Abu Bakar ◽  
Noor Baity Saidi ◽  
Lina Rozano ◽  
Mohd Puad Abdullah ◽  
Suhaina Supian
Keyword(s):  
Disease Resistance ◽  
Plant Disease Resistance ◽  
Carica Papaya ◽  
R Genes ◽  
Binding Surface ◽  
Transcriptome Database ◽  
Defence Signalling ◽  
In Silico Characterization ◽  
Lrr Domain

Disease resistance in plants is commonly associated with resistance (R) genes that encode nucleotide binding site-leucine rich repeat (NBS-LRR) domains that are essential for pathogen recognition and defence signalling. In this study, we identified and analyzed the sequence of putative pathogen-responsive NB-ARC transcripts from Carica papaya transcriptome database, carried out the structural and phylogenetic analysis, and determined the expression profile of the transcripts in C. papaya challenged with Erwinia mallotivora. The findings indicate CpNBS1, the only pathogen-responsive NB-ARC protein identified in this study belongs to the CC-NBS-LRR group. Semi-quantitative PCR showed CpNBS1 was differentially expressed in response to E. mallotivora. Structural analysis of the 4993-Eksotika and 4993-Viorica translated proteins showed striking differences in terms of the number of β-sheets and α-helixes as well their ligand-binding surface, suggesting the role of the LRR domain in determining the specificity of recognition of E. mallotivora effector. Collectively, this study provides new insights into the role of NBS-LRR genes in C. papaya and its implications for enhancing of plant disease resistance through genetic engineering.

scholarly journals Transcriptomic Insights into the Responses in Leaves Storing Lipid Organelles under Different Irradiances

2021 ◽  
Author(s):  
Somrutai Winichayakul ◽  
Richard C. Macknight ◽  
Zac Beechey-Gradwell ◽  
Robyn Lee ◽  
Hong Xue ◽  
...  
Keyword(s):  
Redox Homeostasis ◽  
Co2 Assimilation ◽  
Metabolizable Energy ◽  
Sink Strength ◽  
Low Light ◽  
Signalling Network ◽  
Sugar Signalling ◽  
Biochemical Mechanisms ◽  
Molecular And Biochemical Mechanisms ◽  
Transgenic Ryegrass

To increase the nutritional value of forage, transgenic ryegrass known as High Metabolizable Energy (HME) were previously generated that co-express cysteine-oleosin and diacylglycerol O-acyltransferase. HME not only accumulate lipids in the leaf but also has elevated CO2 assimilation and increased biomass. Shading is one of the most influencing factors for ryegrass growth environments particularly in swards. The aim of this study, therefore, was to determine the influence of irradiance levels on photosynthesis and gene expression in the HME leaves when compared with their corresponding non-transformant (NT). Under low light (150-250 μmol m-2 s-1) and standard light (600-1000 μmol m-2 s-1), the HME accumulated more lipid than NT. The previously reported elevated photosynthesis and increased biomass was observed when the HME were grown under standard light but not under low light. Under both light conditions, compared to NT, the HME had upregulated a number of transcripts involved in lipid metabolism, light capturing, photosynthesis, and sugar signalling network while downregulated genes participated in sugar and fructan biosynthesis. We further discuss how the HME differentially manipulated several genes other metabolic pathways including maintenance of redox homeostasis. Combined, the data suggests that the increased photosynthesis capacity in the HME likely corresponds to an increase of micro-lipid sink strength; these are influenced by available light energy and may be related to diffusional and biochemical activities of stomata. Overall, this work provides a clearly understanding of the changes in molecular and biochemical mechanisms underlying the carbon storing as leaf lipid sink of the HME ryegrass.

A constitutivePR-1::luciferaseexpression screen identifies Arabidopsis mutants with differential disease resistance to both biotrophic and necrotrophic pathogens

2005 ◽  
Vol 6 (1) ◽  
pp. 31-41 ◽  
Author(s):  
SHANE L. MURRAY ◽  
NICOLETTE ADAMS ◽  
DANIEL J. KLIEBENSTEIN ◽  
GARY J. LOAKE ◽  
KATHERINE J. DENBY
Keyword(s):  
Disease Resistance ◽  
Arabidopsis Mutants
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